Difference between revisions of "Team:HZAU-China/Notebook"
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| − | + | <div class="h1">Processing of experiment results</div> | |
| − | + | <h2><b>1.</b> Verification of Poisson distribution</h2> | |
| − | + | <p> | |
| − | + | We infect tumor cells with <i>Salmonella</i> carrying the GFP gene for a period of time and then | |
| − | + | count, and the result is that the distribution of <i>Salmonella</i> in the tumor cells | |
| − | + | is consistent with the Poisson distribution. | |
| − | + | </p> | |
| − | <br><br> | + | <br><br> |
| − | + | <div style="width: 80%; margin: 20px auto"> | |
| − | + | <img src="https://static.igem.org/mediawiki/2018/8/86/T--HZAU-China--Notebook-fig1.png" width="100%" alt=""> | |
| − | + | </div> | |
| − | + | <p style="width: 100%; text-align: center !important;"><b>Figure 1.</b> Infection of <i>Salmonella</i> | |
| − | + | and the result and result of analysis.</p><br> | |
| − | + | <p>The amount of cells and bacteria is obtained through experiments. | |
| − | + | </p><br> | |
| − | + | </div> | |
| − | + | <div id="float02"> | |
| − | + | <p>The concentration of bacteria is converted according to MOI and cell density.<br> | |
| − | <br> | + | We adjusted As to match the curve to the results of the Salmonella infection experiment. When As is |
| − | The ‘Nsal’ and ‘Tumor’ is already known before the experiment. When the parameter ‘As’ is 0.0003, the model results fit the experimental results. The red points are corresponding experimental results. </p> | + | equal to 0.0003, the curve simulated by the mathematical model matches the experimental results. |
| + | </p> | ||
| + | <div style="width: 80%; margin: 20px auto"> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/8/84/T--HZAU-China--Notebook-fig2.png" width="100%" alt=""> | ||
| + | </div> | ||
| + | <p style="width: 100%; text-align: center !important;"><b>Figure 2.</b> Parameter fitting of the tumor | ||
| + | cells infection experiments. | ||
| + | </p><br> | ||
| + | <p> | ||
| + | The ‘Nsal’ and ‘Tumor’ is already known before the experiment. When the parameter ‘As’ is 0.0003, | ||
| + | the model results fit the experimental results. The red points are corresponding experimental | ||
| + | results. </p> | ||
</div> | </div> | ||
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<span class="biaoti">Improve</span> | <span class="biaoti">Improve</span> | ||
| + | <span class="xsjPic"><img src="https://static.igem.org/mediawiki/2018/7/7c/T--HZAU-China--ysj.svg" alt=""></span> | ||
| + | </div> | ||
| + | <a class="daohangyi" href="https://2018.igem.org/Team:HZAU-China/InterLab"> | ||
| + | <span class="biaoti">InterLab</span> | ||
| + | <span class="xsjPic"><img src="https://static.igem.org/mediawiki/2018/7/7c/T--HZAU-China--ysj.svg" alt=""></span> | ||
| + | </a> | ||
| + | <div class="daohangyi" href="#"> | ||
| + | <span class="biaoti">Notebook</span> | ||
<span class="xsjPic"><img src="https://static.igem.org/mediawiki/2018/8/8d/T--HZAU-China--xjt.svg" alt=""></span> | <span class="xsjPic"><img src="https://static.igem.org/mediawiki/2018/8/8d/T--HZAU-China--xjt.svg" alt=""></span> | ||
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Revision as of 21:08, 17 October 2018
Processing of experiment results
1. Verification of Poisson distribution
We infect tumor cells with Salmonella carrying the GFP gene for a period of time and then count, and the result is that the distribution of Salmonella in the tumor cells is consistent with the Poisson distribution.
Figure 1. Infection of Salmonella and the result and result of analysis.
The amount of cells and bacteria is obtained through experiments.
The concentration of bacteria is converted according to MOI and cell density.
We adjusted As to match the curve to the results of the Salmonella infection experiment. When As is
equal to 0.0003, the curve simulated by the mathematical model matches the experimental results.
Figure 2. Parameter fitting of the tumor cells infection experiments.
The ‘Nsal’ and ‘Tumor’ is already known before the experiment. When the parameter ‘As’ is 0.0003, the model results fit the experimental results. The red points are corresponding experimental results.
