Difference between revisions of "Team:Bio Without Borders/Notebook"

 
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<h1>Notebook</h1>
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<p> This page contains the following links to our <b><i>protocols</i></b> page, which highlights all the protocols we used in our experimentation, as well the <b><i>labbook</i></b> page, which documents what we performed in the lab. </p>
 
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<a href="https://2018.igem.org/Team:Bio_Without_Borders/Protocols">Protocols</a>
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<dt><b><font color="#009999">Week 1 (June 4-8)</font></b></dt>
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<a href="https://2018.igem.org/Team:Bio_Without_Borders/LabBook">Lab Book</a>
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<dd>•Made competent cells</dd>
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<dt><b><font color="#009999">Week 2 (June 11-15)</font></b></dt>
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<dd>•Testing competent cells using iGem DNA transformation samples.</dd>
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<dd>• Tested to see if they grew better on a freezer block versus an ice bath.</dd>
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<dd>• Ran colony PCR and plated bacteria on antimicrobial plate. </dd>
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<dd>• cPCR, chloramphenicol, and culturing protocols, made agarose gel </dd>  
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<dd>• gel electrophoresis for cPCR, nucleic acid purification plasmid </dd>
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<dt><b><font color="#009999">Week 3 (June 18-22)</font></b></dt>
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<dd>•Made chloramphenicol LB plates</dd>
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<dd>•Restriction enzyme digest of iGem plasmid using EcoRI and PstI</dd>
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<dd>•Amplification of iGem backbones</dd>
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Latest revision as of 21:30, 17 October 2018

Notebook

This page contains the following links to our protocols page, which highlights all the protocols we used in our experimentation, as well the labbook page, which documents what we performed in the lab.

Protocols Lab Book