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| | <h1 style="font-family:helvetica;">Notebook</h1> | | <h1 style="font-family:helvetica;">Notebook</h1> |
| − | | + | <p> This page contains the following links to our <b><i>protocols</i></b> page, which highlights all the protocols we used in our experimentation, as well the <b><i>labbook</i></b> page, which documents what we performed in the lab. </p> |
| | </div> | | </div> |
| | <div class="clear"></div> | | <div class="clear"></div> |
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| | <body> | | <body> |
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| − | <dl> | + | <a href="https://2018.igem.org/Team:Bio_Without_Borders/Protocols">Protocols</a> |
| − | <dt><b><font color="#009999">Week 1 (June 4-8)</font></b></dt>
| + | <a href="https://2018.igem.org/Team:Bio_Without_Borders/LabBook">Lab Book</a> |
| − | <dd>•Made competent cells</dd>
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| − | <dt><b><font color="#009999">Week 2 (June 11-15)</font></b></dt>
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| − | <dd>•Testing competent cells using iGem DNA transformation samples.</dd> | + | |
| − | <dd>• Tested to see if they grew better on a freezer block versus an ice bath.</dd>
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| − | <dd>• Ran colony PCR and plated bacteria on antimicrobial plate. </dd>
| + | |
| − | <dd>• cPCR, chloramphenicol, and culturing protocols, made agarose gel </dd>
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| − | <dd>• gel electrophoresis for cPCR, nucleic acid purification plasmid </dd>
| + | |
| − | <dt><b><font color="#009999">Week 3 (June 18-22)</font></b></dt>
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| − | <dd>•Made chloramphenicol LB plates</dd>
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| − | <dd>•Restriction enzyme digest of iGem plasmid pSB1C3 using EcoR1 and Pst1</dd>
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| − | <dd>•Made digest master mix; ran an ezyme digest of iGem plasmids pSB1A3 and pSB1K3
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| − | Performed ligation of </dd>
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| − | <dd>pSB1A3 and pSB1K3; transformed pSB1A3 in competent cells on ampicillin plate</dd>
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| − | <dd>•Created a 50mg/mL kanamycin solution and then alloquoted it to about 80 1.5mL tubes. </dd>
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| − | <dd>•Inoculated LB with ampicillin colonies (with insert).</dd>
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| − | <dd>•Streaked ampicillin (1A3) colony onto new plates.</dd>
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| − | <dd>•Conducted cPCR for 1A3 colonies with insert.</dd>
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| − | <dt><b><font color="#009999">Week 4 (June 25-29)</font></b></dt>
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| − | <dd>•Conducted cPCR for PSB1K3.</dd>
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| − | <dd>•Ran 1% agarose gel of pSB1A3 with the insert (colonies that were re-streaked).</dd>
| + | |
| − | <dd>•Ran 1% agarose gel of pSB1K3 with insert.</dd>
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| − | <dd>•Cleaned laboratory material.
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| − | <dd>•Set up PCR using Q5 mastermix.</dd>
| + | |
| − | <dd>•Amplification of pSB1C3 plasmid backbone.</dd>
| + | |
| − | <dd>•Set up phusion PCR and gel electrophoresis of PSB1A3.
| + | |
| − | <dd>•TMR!!! Amplification of pSB1C3 and pSB1A3, ran it through PCR cleanup and digested with EcoR1 and Pst1.</dd>
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| | </dl> | | </dl> |
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