Difference between revisions of "Team:East Chapel Hill/Measurement"

 
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<h1>Measurement</h1>
 
<h1>Measurement</h1>
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<p2 style="font-size:18px;"> Our CHOP II system is an operon that is valuable in measuring the responsiveness of fluoride riboswitches as well as qualitatively measuring fluoride concentrations in water.
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One may easily determine the responsiveness of a fluoride riboswitch to fluoride by simply cloning the riboswitch of interest into the operon using the Gibson homology overhangs included in the sequence of the part. To determine the responsiveness of the riboswitch of interest, one may plate the operon containing <i>Δ</i>-<i>crcB</i> (fluoride efflux pump) <i>E. coli</i> onto agar plates with varying levels of fluoride. This technique can be applied to test at which levels of fluoride other riboswitches are most responsive.
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Additionally, our operon is valuable in qualitatively measuring amounts of fluoride in water. To qualitatively determine the amount of fluoride in a water sample, one may make agar plates with the water being tested and grow <I>Δ</I>-<i>crcB E. coli</i> containing BBa_K2843000 with the <i>B. cereus</i> riboswitch. This allows for a relatively simple and replicable device to qualitatively measure fluoride levels in water.
  
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<p>There are a lot of exciting parts in the Registry, but many parts have still not been characterized. Synthetic Biology needs great measurement approaches for characterizing new parts, and efficient new methods for characterizing many parts at once. If you've done something exciting in the area of Measurement, describe it here!</p>
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<br></br>
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In the images below you are able to see growth of <I>Δ</I>-<i>crcB E. coli</i> increase with increasing levels of fluoride, demonstrating its value as a device for qualitative measurement of excess fluoride in water.
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<img src="https://static.igem.org/mediawiki/2018/9/98/T--East_Chapel_Hill--RESULTSPAGE1.png" </img>
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<img src="https://static.igem.org/mediawiki/2018/0/08/T--East_Chapel_Hill--RESULTSPAGE2.png" </img>
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<h3>Best Innovation in Measurement Special Prize</h3>
 
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<p>If you've done excellent work in measurement, you should consider nominating your team for this special prize. Designing great measurement approaches for characterizing new parts or developing and implementing an efficient new method for characterizing thousands of parts are good examples.
 
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To compete for the <a href="https://2018.igem.org/Judging/Awards">Best Innovation in Measurement prize</a>, please describe your work on this page and also fill out the description on the <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a>.
 
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You must also delete the message box on the top of this page to be eligible for this prize.
 
  
 
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<h3>Inspiration</h3>
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<p>You can look at what other teams did to get some inspiration! <br />
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Here are a few examples:</p>
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<li><a href="https://2016.igem.org/Team:Stanford-Brown">2016 Stanford-Brown</a></li>
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<li><a href="https://2016.igem.org/Team:Genspace">2016 Genspace</a></li>
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<li><a href="https://2015.igem.org/Team:William_and_Mary">2015 William and Mary</a></li>
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<li><a href="https://2014.igem.org/Team:Aachen">2014 Aachen  </a></li>
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Latest revision as of 21:36, 17 October 2018

East Chapel Hill

Measurement

Our CHOP II system is an operon that is valuable in measuring the responsiveness of fluoride riboswitches as well as qualitatively measuring fluoride concentrations in water.

One may easily determine the responsiveness of a fluoride riboswitch to fluoride by simply cloning the riboswitch of interest into the operon using the Gibson homology overhangs included in the sequence of the part. To determine the responsiveness of the riboswitch of interest, one may plate the operon containing Δ-crcB (fluoride efflux pump) E. coli onto agar plates with varying levels of fluoride. This technique can be applied to test at which levels of fluoride other riboswitches are most responsive.

Additionally, our operon is valuable in qualitatively measuring amounts of fluoride in water. To qualitatively determine the amount of fluoride in a water sample, one may make agar plates with the water being tested and grow Δ-crcB E. coli containing BBa_K2843000 with the B. cereus riboswitch. This allows for a relatively simple and replicable device to qualitatively measure fluoride levels in water.

In the images below you are able to see growth of Δ-crcB E. coli increase with increasing levels of fluoride, demonstrating its value as a device for qualitative measurement of excess fluoride in water.