Difference between revisions of "Team:GO Paris-Saclay/labnotebook"

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This is the lab notebook portal. From here you can access our complete written notes regarding our experimental procedures. It is divided by general goal, like establishing a bistable population. While we aimed to be as clear as possible, do not hesitate to contact us if you have any question
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This is the lab notebook portal. From here you can access our complete written notes regarding our experimental procedures.  
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It is divided by general goal, like establishing a bistable population.
  
 
== General procedures ==
 
== General procedures ==
 
The protocols used for the day to day stuff, like what PCR program, or how our cells were made competent.
 
The protocols used for the day to day stuff, like what PCR program, or how our cells were made competent.
  
 
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[[Team:GO_Paris-Saclay/labnotebook-protocols | Standard protocols]]
[[labnotebook/Heat_Shock_Transformation | Heat Shock Transformation of DH5alpha]]
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== Bistable population ==
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The making of the bistable, bimodal distribution of bacteria in our bioreactor.
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Cloning of the import, transformation and degradation enzymes, and their characterization.
 
Cloning of the import, transformation and degradation enzymes, and their characterization.
  
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[[Team:GO_Paris-Saclay/labnotebook-cpg2 | Cloning cpg2]]
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[[Team:GO_Paris-Saclay/labnotebook-folc | Cloning folC]]
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[[Team:GO_Paris-Saclay/labnotebook-cpg2-folc | Cloning cpg2 and folC together]]
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== HeteroGenious population ==
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The making of the bimodal distribution of bacteria in our bioreactor.
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[[Team:GO_Paris-Saclay/labnotebook-ler | Cloning ler]]
  
== Auxilliary goals ==
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[[Team:GO_Paris-Saclay/labnotebook-lee5 | Cloning LEE5]]
Various other entries...
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Latest revision as of 21:40, 17 October 2018

This is the lab notebook portal. From here you can access our complete written notes regarding our experimental procedures.

It is divided by general goal, like establishing a bistable population.

General procedures

The protocols used for the day to day stuff, like what PCR program, or how our cells were made competent.

Standard protocols


MTX degradation

Cloning of the import, transformation and degradation enzymes, and their characterization.

Cloning cpg2

Cloning folC

Cloning cpg2 and folC together

HeteroGenious population

The making of the bimodal distribution of bacteria in our bioreactor.

Cloning ler

Cloning LEE5