Difference between revisions of "Team:OUC-China/Description"

 
(42 intermediate revisions by 5 users not shown)
Line 16: Line 16:
 
position:absolute;
 
position:absolute;
 
top:0 !important;
 
top:0 !important;
−
 
+
width:100%;
 
}
 
}
  
Line 35: Line 35:
 
}
 
}
  
−
 
+
 +
.nb{
 +
background-color:#F7F7F7;
 +
}
 +
 +
 +
.sk{
 +
width:1000px;
 +
padding:0px 50px 0px 50px;
 +
    margin-left: auto;
 +
    margin-right: auto;
 +
background-color:white;
 +
}
 +
  
 
#myBtn {
 
#myBtn {
 
   display: none;
 
   display: none;
 
   position: fixed;
 
   position: fixed;
−
   bottom: 20px;
+
   bottom: 5px;
 
   right: 30px;
 
   right: 30px;
 
   z-index: 99;
 
   z-index: 99;
Line 102: Line 115:
 
</ul>
 
</ul>
 
</li>
 
</li>
−
<li><a href="#">Human_Practices</a>
+
<li><a href="#">HP</a>
 
<ul>
 
<ul>
−
<li><a href="https://2018.igem.org/Team:OUC-China/Human_Practices">Human_Practices</a></li>
+
<li><a href="https://2018.igem.org/Team:OUC-China/Human_Practices">Human Practices</a></li>
−
<li><a href="https://2018.igem.org/Team:OUC-China/Public_Engagement">Public_Engagement</a></li>
+
<li><a href="https://2018.igem.org/Team:OUC-China/Public_Engagement">Public Engagement</a></li>
 
</ul>
 
</ul>
 
</li>
 
</li>
 
<li><a href="#">Team</a>
 
<li><a href="#">Team</a>
 
<ul>
 
<ul>
−
<li><a href="https://2018.igem.org/Team:OUC-China/Team">Team_Member</a></li>
+
<li><a href="https://2018.igem.org/Team:OUC-China/Team">Team Member</a></li>
 
<li><a href="https://2018.igem.org/Team:OUC-China/Collaborations">Collaborations</a></li>
 
<li><a href="https://2018.igem.org/Team:OUC-China/Collaborations">Collaborations</a></li>
 
<li><a href="https://2018.igem.org/Team:OUC-China/Attributions">Attributions</a></li>
 
<li><a href="https://2018.igem.org/Team:OUC-China/Attributions">Attributions</a></li>
Line 122: Line 135:
 
<!-- Banner -->
 
<!-- Banner -->
 
 
−
<img src="https://static.igem.org/mediawiki/2018/3/3a/T--OUC-China--overvierw.jpg"alt="banner"width="100%">
+
<img src="https://static.igem.org/mediawiki/2018/8/88/T--OUC-China--descri.jpg"alt="banner"width="100%">
 
 
 
</div>
 
</div>
 
<!-- Main -->
 
<!-- Main -->
−
<div id="main" class="container">
+
<div class="nb">
 
 
 
 
 
 
−
<div class="12u">
+
<div class="sk">
  
 
<!-- Features -->
 
<!-- Features -->
Line 138: Line 151:
 
<p align="justify">
 
<p align="justify">
  
−
<h3>When we started</h3>
+
<div align="center"><img src="https://static.igem.org/mediawiki/2018/0/0a/T--OUC-China--project-_overview-upupup2.jpg"
−
<br />&emsp;&emsp;The genome contains commands which help the cell to realize some basic functions like eat, reproduce, communicate, move and excrete. Cells also can be programmed by exogenous DNA which have new commands that guide the cell to perform new artificial tasks and aims in parallel. As we all know, synthetic biology developed very fast in recent years. One of the most important things that helps us to build our fantastic dream in synthetic biology is to develop small modular genetic parts. By doing, the whole circuit will become more flexible and easy to operate. Genetic engineering is entering a new era, where microorganisms can be programmed by using synthetic constructs. More and more toolbox of modular genetic parts is designed to interface with the control of cellular processes. As we all know, the well-designed modular parts need to be assembled into biological circuits or pathways easily and different parts usually have different functions. The part created to regulate downstream gene expression is common and useful.  
+
 
 +
width="800"></div>
 +
 
 +
<h3><font color="#008B45">When we started</font></h3>
 +
<br />The genome of organisms contains commands for basic biological functions like assimilation, reproduction, communication, motility and dissimilation. Meanwhile, cells can also be reprogrammed by exogenous DNA with new genetic information for artificial behaviours and functions. As we know, synthetic biology developed very fast in recent years. One of the most important mission that helps us to build our fantastic dream in synthetic biology is to develop standardized gene regulatory parts. By doing this, the function of artificial circuits will become more predictable and operable. More and more toolbox of modular genetic parts were designed to regulate cellular processes. The gene regulatory toolbox for controlling and remodelling genetic circuits is essential and valuable.
 
</p>
 
</p>
 
<p>
 
<p>
−
<h3>The existing strategies</h3>
+
<div align="center"><img src="https://static.igem.org/mediawiki/2018/f/ff/T--OUC-China--project-_overview.png"
−
<br />&emsp;&emsp;To meet different needs, scientists have already created a lot of genetic parts based on three levels, DNA, RNA and protein. Among those three levels, post- transcriptional regulation plays a main role of gene expression. Post-translational regulation refers to the control of the levels of active protein. There are several forms. It is performed either by means of reversible events (post-translational modifications, such as phosphorylation or sequestration) or by means of irreversible events (proteolysis). To solve some current problems, more and more methods have been created based on post-translational regulation because of flexibility and practicability. The more useful toolkits we design, the more possibilities we can have.
+
 
 +
width="800"></div>
 +
<br /><br />
 +
<h3><font color="#008B45">The existing strategies</font></h3>
 +
<br />To satisfy various requirements, scientists have already created a lot of gene regulation tools based on three levels: DNA, RNA and protein. Among above three levels, post-transcriptional regulation plays a critical role for gene expression control. Post-transcriptional regulation generally refers to the control on RNA molecules. There are several advantages of post-transcriptional regulation comparing with traditional genetic regulation methods on DNA, like faster response time, simpler regulatory mechanism and diverse design strategies. More and more methods have been created based on post-transcriptional regulation because of its flexibility and practicability [1][2][3].  
 
<br />
 
<br />
−
<br />&emsp;&emsp;Some methods have been created like riboswitch. A riboswitch is a regulatory segment of a messenger RNA molecule that binds a small molecule, resulting in a change in production of the proteins encoded by the mRNA. Thus, a mRNA that contains a riboswitch is directly involved in regulating its own activity, in response to the concentrations of its effector molecule. It's not only a test system, but also a way to open or close the expression of downstream genes.  
+
<br />A typical case is riboswitch which can bind to a small molecule and result in a secondary structure change of mRNA with translation rate tuning of corresponding protein. It is not only a potential biosensor design platform, but also a powerful tool to control the expression level of downstream genes. 
 +
<br />
 +
<br />RNA switch responsive to small RNA is another type. Small RNA could also be engineered as an activator, which is able to regulate the cis-repressive RNA (crRNA) element that masks the ribosome binding site (RBS) of the transcript of particular gene through base pairing [4]. A well-known case named toehold switch has been applied on synthetic biology.
 +
<br /><br />
 +
Recently, a CRISPR-Csy4 mediated activation method based on cis-repressive RNA cleavage has been achieved in bacteria [5]. This system significantly improved the feasibility of post-transcriptional regulation.
 
<br />
 
<br />
−
<br />&emsp;&emsp;Small RNA based repressor or activator is also an example. They employed a cis-repressive RNA (crRNA) element that masks the ribosome binding site (RBS) of a reporter gene through base pairing. An ectopically expressed trans-activating RNA was then designed to compete the binding with crRNA and thus to unmask RBS and restore translation.
 
 
</p>
 
</p>
 
<p>
 
<p>
−
<h3>Some problems of current methods</h3>
+
<div align="center"><img src="https://static.igem.org/mediawiki/2018/f/ff/T--OUC-China--project-_overview.png"
−
<br />&emsp;&emsp;But some of them may have weaknesses in design. For small RNA, if the crRNA is perfectly paired with RBS, the transactivating RNA cannot compete the binding with crRNA. This may cause the low efficiency of opening this kind of switches. As for toehold switch, it is not hard to find out that each switch needs to be individually designed. At the same time, this may cause insertion of undesirable foreign sequences.
+
 
 +
width="800"></div>
 +
<br /><br />
 +
 
 +
<h3><font color="#008B45">OUR INNOVATION AND ADVANTAGES</font></h3>
 +
<br />By reviewing existing methods and strategies, we are aware of some aspects worth optimizing. For general small RNA-mediated riboswitch, if the crRNA is perfectly paired with RBS, the trans-activating RNA will be hard to compete for the binding with crRNA. This may cause low activation efficiency for opening this type of switch. As for toehold switch, each switch needs to be individually designed. At the same time, introducing long homologous sequence for regulation may increase undesired recombination risks of foreign fragment insertion. [6].  
 
<br />
 
<br />
−
<br />&emsp;&emsp;This year, our new alternative regulatory method is the ‘MiniToe Family’, which is a new toolkit based on csy4 produced by OUC-China.
+
<br />This year, we designed and achieved a new alternative regulatory tool named "MiniToe Family", which is a new toolkit based on Csy4 produced by OUC-China.
 
</p>
 
</p>
 
<p>
 
<p>
−
<h3>Our journey</h3>
+
<div align="center"><img src="https://static.igem.org/mediawiki/2018/f/ff/T--OUC-China--project-_overview.png"
 +
 
 +
width="800"></div> 
 +
<br /><br />
 +
 
 +
<h3><font color="#008B45">OUR JOURNEY</font></h3>
 
<br />
 
<br />
−
  <div align="center"><img src="https://static.igem.org/mediawiki/2018/5/59/T--OUC-China--liucheng.png"  ></div>  
+
  <div align="center"><img src="https://static.igem.org/mediawiki/2018/5/59/T--OUC-China--liucheng.png"width="70%"  ></div>  
 
<br />
 
<br />
 
</p>
 
</p>
 
<p>
 
<p>
−
<h3>Some problems of current methods</h3>
+
 
 +
<div align="center"><img src="https://static.igem.org/mediawiki/2018/f/ff/T--OUC-China--project-_overview.png"
 +
 
 +
width="800"></div>
 +
<br /><br />
 +
 
 +
<h3><font color="#008B45">WE HAVE DONE</font></h3>
 
<br /><br />★We successfully registered our team for iGEM at February 8th.
 
<br /><br />★We successfully registered our team for iGEM at February 8th.
 
<br /><br />★We met all deliverables on the Competition Deliverables page.
 
<br /><br />★We met all deliverables on the Competition Deliverables page.
−
<br /><br />★We made a detailed description on what is done by ourselves and what supported by others with precise attribution.(<a href="https://2018.igem.org/Team:OUC-China/Attributions"> https://2018.igem.org/Team:OUC-China/Attributions</a>)
+
<br /><br />★We made a detailed description on what is done by ourselves and what is supported by others with precise attribution.<a href="https://2018.igem.org/Team:OUC-China/Attributions"> Click here!</a>
−
<br /><br />★We participated in the Interlab Measurement and submitted our result which was accepted by committee.(<a href="https://2018.igem.org/Team:OUC-China/InterLab">https://2018.igem.org/Team:OUC-China/InterLab</a>)
+
<br /><br />★We participated in the Interlab Measurement and submitted our result which was accepted by committee.<a href="https://2018.igem.org/Team:OUC-China/InterLab"> Click here!</a>
−
<br /><br />★We submitted 20 new Biobrick Parts designed by ourselves which play significant roles in our project and we send 12 new Biobrick Parts among them.(<a href="https://2018.igem.org/Team:OUC-China/Parts">https://2018.igem.org/Team:OUC-China/Parts</a>)
+
<br /><br />★We submitted 20 new Biobrick Parts designed by ourselves which play significant roles in our project and we sent 12 new Biobrick Parts among them.<a href="https://2018.igem.org/Team:OUC-China/Parts"> Click here!</a>
−
<br /><br />★We have many kinds of collaborations related to our project! More than twelve teams contact us and help us translate our comic book into other fourteen languages. More than fifteen teams take our comic book——《E.coli SPACESHIP》to local areas for Science education. Four team help us test the function of our parts and we also help them test their system. Four other teams and OUC-china form the basic research results transformation team. We share survey experience and form a document, providing future iGEMers a reference. A new team is formed successful with our help.(<a href="https://2018.igem.org/Team:OUC-China/Collaborations">https://2018.igem.org/Team:OUC-China/Collaborations</a>)
+
<br /><br />★We have many kinds of collaborations related to our project! Foreign iGEM teams contacted us and helped us translate our comic book into 13 languages. More than 14 teams take our comic book——《<i>E. coli SPACESHIP</i>》to local areas for Science education. 4 team helped us test the function of our parts and we also helped them test their system. Three other teams and OUC-China formed the fundamental research results transformation group. We shared survey experience and formed a document, providing future iGEMers a reference. A new team was formed successfully with our help.<a href="https://2018.igem.org/Team:OUC-China/Collaborations"> Click here!</a>
−
<br /><br />★We carefully confirmed that our work is safe and do not harm to the environment and society!(<a href="https://2018.igem.org/Team:OUC-China/Safety">https://2018.igem.org/Team:OUC-China/Safety</a>)
+
<br /><br />★We carefully confirmed that our work is safe and do not harm to the environment and society!<a href="https://2018.igem.org/Team:OUC-China/Safety"> Click here!</a>
−
<br /><br />★We spread iGEM spirits and promoted the development of synthetic biology in China through popular science comic book——《E.coli SPACESHIP》! And we translate the book into several languages and spread it to many areas with the help of other iGEM teams! We also spread iGEM spirits by synthetic biology lecture, summer camp, and social media.(<a href="https://2018.igem.org/Team:OUC-China/Public_Engagement"> https://2018.igem.org/Team:OUC-China/Public_Engagement</a>)
+
<br /><br />★We spread iGEM spirits and promoted the development of synthetic biology in China through popular science comic book——《<i>E. coli SPACESHIP</i>》! And we translated the book into several languages and spread it to many areas with the help of other iGEM teams! We also spread iGEM spirits by synthetic biology lecture, summer camp, and social media.<a href="https://2018.igem.org/Team:OUC-China/Public_Engagement"> Click here!</a>
−
<br /><br />★We participated in synthetic biology forums —— Conference of China iGEMer Community (CCiC) as the co-organizer. (<a href="https://2018.igem.org/Team:OUC-China/Human_Practices">https://2018.igem.org/Team:OUC-China/Human_Practices</a>)
+
<br /><br />★We participated in synthetic biology forums —— Conference of China iGEMer Community (CCiC) as the co-organizer. <a href="https://2018.igem.org/Team:OUC-China/Human_Practices"> Click here!</a>
−
<br /><br />★We improved Part BBa_K1062004（此part链接）by point mutant it into several mutations and have tested them by data support. They have functional improvement and both of them are the main roles of our system! See more details in this page! (<a href="https://2018.igem.org/Team:OUC-China/Improve"> https://2018.igem.org/Team:OUC-China/Improve</a>) BBa_K2615004, BBa_K2615005, BBa_K2615006, BBa_K2615007.
+
<br /><br />★We improved Part（<a href="http://parts.igem.org/Part:BBa_K1062004">BBa_K1062004</a>）by point mutation and tested them by data support. They have functional improvement and both of them are the main roles of our system! The new parts we improved are BBa_K2615004, BBa_K2615005, BBa_K2615006, BBa_K2615007.  See more details in this page! <a href="https://2018.igem.org/Team:OUC-China/Improve"> Click here!</a>
−
<br /><br />★The models play an important role on our project and influence our project deeply this year. For the first system, we built an ODE model and then the modeler helps us select mutants for second system. In miniToe polycistron, the model also be used to build a frame of whole structure. (<a href="https://2018.igem.org/Team:OUC-China/Model">https://2018.igem.org/Team:OUC-China/Model</a>)
+
<br /><br />★The models play an important role in our project and influenced our project deeply this year. For the first system, we built an ODE model and then the modeler helped us select mutants for second system. In miniToe polycistron, the model was also used to build a frame of whole structure. <a href="https://2018.igem.org/Team:OUC-China/Model"> Click here!</a>
−
<br /><br />★We successfully apply our system to regulation of gene, motA!(<a href="https://2018.igem.org/Team:OUC-China/Demonstrate"> https://2018.igem.org/Team:OUC-China/Demonstrate</a>)
+
<br /><br />★We successfully applied our system to the regulation of gene, motA!<a href="https://2018.igem.org/Team:OUC-China/Demonstrate"> Click here!</a>
 
<br />
 
<br />
  
 
</p>
 
</p>
  
 +
<p>
  
 +
<div align="center"><img src="https://static.igem.org/mediawiki/2018/f/ff/T--OUC-China--project-_overview.png"
 +
 +
width="800"></div>
 +
<br /><br />
 +
 +
<h3>reference</h3>
 +
[1]. Glisovic T, Bachorik J L, Yong J, et al. <i>RNA‐binding proteins and post‐transcriptional gene regulation</i>[J]. Febs Letters, 2008, 582(14):1977-1986.
 +
<br /><br />
 +
[2]. Bifulco P, Cesarelli M, Cerciello T, et al. <i>Mechanisms of miRNA-mediated post-transcriptional regulation in animal cells</i>[J]. Current Opinion in Cell Biology, 2009, 21(3):452-460.
 +
<br /><br />
 +
[3]. Lai E C.<i> Micro RNAs are complementary to 3' UTR sequence motifs that mediate negative post-transcriptional regulation.</i>[J]. Nature Genetics, 2002, 30(4):363-364.
 +
<br /><br />
 +
[4]. Karagiannis, P., Y. Fujita, and H. Saito.,<i>RNA-based gene circuits for cell regulation.</i> Proc Jpn Acad Ser B Phys Biol Sci, 2016. 92(9): p. 412-422.
 +
<br /><br />
 +
[5]. Du, P., et al.,<i> Engineering Translational Activators with CRISPR-Cas System.</i> ACS Synth Biol, 2016. 5(1): p. 74-80.
 +
<br /><br />
 +
[6]. Green A, Silver P, Collins J, et al.<i> Toehold switches: de-novo-designed regulators of gene expression.</i>[J]. Cell, 2014, 159(4):925-939.
 +
<br /><br /><br /><br /><br /><br />
 +
</p>
 
</section>
 
</section>
  
Line 205: Line 265:
  
  
−
   <div class="copyright1">Contact Us : oucigem@163.com  | &copy;2018 OUC IGEM.All Rights Reserved.  |  ………… </div>
+
   <div class="copyright1">Contact Us : oucigem@163.com  | &copy;2018 OUC IGEM.All Rights Reserved. <br />
 +
<img src="https://static.igem.org/mediawiki/2017/b/b4/T--OUC-China--foot1.jpeg"alt="banner"width="80px">
 +
<img src="https://static.igem.org/mediawiki/2017/6/62/T--OUC-China--foot2.jpeg"alt="banner"width="80px">
 +
<img src="https://static.igem.org/mediawiki/2018/f/f3/T--OUC-China--lalala.png"alt="banner"width="80px">
 +
<img src="https://static.igem.org/mediawiki/2017/5/51/T--OUC-China--NSG.png"alt="banner"height="65px">
 +
<img src="https://static.igem.org/mediawiki/2017/2/2a/T--OUC-China--ML.png"alt="banner"height="65px">&emsp;
 +
  </div>
 
 
  

Latest revision as of 21:47, 17 October 2018

Team OUC-China: Main
banner

Overview

When we started


The genome of organisms contains commands for basic biological functions like assimilation, reproduction, communication, motility and dissimilation. Meanwhile, cells can also be reprogrammed by exogenous DNA with new genetic information for artificial behaviours and functions. As we know, synthetic biology developed very fast in recent years. One of the most important mission that helps us to build our fantastic dream in synthetic biology is to develop standardized gene regulatory parts. By doing this, the function of artificial circuits will become more predictable and operable. More and more toolbox of modular genetic parts were designed to regulate cellular processes. The gene regulatory toolbox for controlling and remodelling genetic circuits is essential and valuable.



The existing strategies


To satisfy various requirements, scientists have already created a lot of gene regulation tools based on three levels: DNA, RNA and protein. Among above three levels, post-transcriptional regulation plays a critical role for gene expression control. Post-transcriptional regulation generally refers to the control on RNA molecules. There are several advantages of post-transcriptional regulation comparing with traditional genetic regulation methods on DNA, like faster response time, simpler regulatory mechanism and diverse design strategies. More and more methods have been created based on post-transcriptional regulation because of its flexibility and practicability [1][2][3].

A typical case is riboswitch which can bind to a small molecule and result in a secondary structure change of mRNA with translation rate tuning of corresponding protein. It is not only a potential biosensor design platform, but also a powerful tool to control the expression level of downstream genes.

RNA switch responsive to small RNA is another type. Small RNA could also be engineered as an activator, which is able to regulate the cis-repressive RNA (crRNA) element that masks the ribosome binding site (RBS) of the transcript of particular gene through base pairing [4]. A well-known case named toehold switch has been applied on synthetic biology.

Recently, a CRISPR-Csy4 mediated activation method based on cis-repressive RNA cleavage has been achieved in bacteria [5]. This system significantly improved the feasibility of post-transcriptional regulation.



OUR INNOVATION AND ADVANTAGES


By reviewing existing methods and strategies, we are aware of some aspects worth optimizing. For general small RNA-mediated riboswitch, if the crRNA is perfectly paired with RBS, the trans-activating RNA will be hard to compete for the binding with crRNA. This may cause low activation efficiency for opening this type of switch. As for toehold switch, each switch needs to be individually designed. At the same time, introducing long homologous sequence for regulation may increase undesired recombination risks of foreign fragment insertion. [6].

This year, we designed and achieved a new alternative regulatory tool named "MiniToe Family", which is a new toolkit based on Csy4 produced by OUC-China.



OUR JOURNEY





WE HAVE DONE



★We successfully registered our team for iGEM at February 8th.

★We met all deliverables on the Competition Deliverables page.

★We made a detailed description on what is done by ourselves and what is supported by others with precise attribution. Click here!

★We participated in the Interlab Measurement and submitted our result which was accepted by committee. Click here!

★We submitted 20 new Biobrick Parts designed by ourselves which play significant roles in our project and we sent 12 new Biobrick Parts among them. Click here!

★We have many kinds of collaborations related to our project! Foreign iGEM teams contacted us and helped us translate our comic book into 13 languages. More than 14 teams take our comic book——《E. coli SPACESHIP》to local areas for Science education. 4 team helped us test the function of our parts and we also helped them test their system. Three other teams and OUC-China formed the fundamental research results transformation group. We shared survey experience and formed a document, providing future iGEMers a reference. A new team was formed successfully with our help. Click here!

★We carefully confirmed that our work is safe and do not harm to the environment and society! Click here!

★We spread iGEM spirits and promoted the development of synthetic biology in China through popular science comic book——《E. coli SPACESHIP》! And we translated the book into several languages and spread it to many areas with the help of other iGEM teams! We also spread iGEM spirits by synthetic biology lecture, summer camp, and social media. Click here!

★We participated in synthetic biology forums —— Conference of China iGEMer Community (CCiC) as the co-organizer. Click here!

★We improved Part（BBa_K1062004）by point mutation and tested them by data support. They have functional improvement and both of them are the main roles of our system! The new parts we improved are BBa_K2615004, BBa_K2615005, BBa_K2615006, BBa_K2615007. See more details in this page! Click here!

★The models play an important role in our project and influenced our project deeply this year. For the first system, we built an ODE model and then the modeler helped us select mutants for second system. In miniToe polycistron, the model was also used to build a frame of whole structure. Click here!

★We successfully applied our system to the regulation of gene, motA! Click here!



reference

[1]. Glisovic T, Bachorik J L, Yong J, et al. RNA‐binding proteins and post‐transcriptional gene regulation[J]. Febs Letters, 2008, 582(14):1977-1986.

[2]. Bifulco P, Cesarelli M, Cerciello T, et al. Mechanisms of miRNA-mediated post-transcriptional regulation in animal cells[J]. Current Opinion in Cell Biology, 2009, 21(3):452-460.

[3]. Lai E C. Micro RNAs are complementary to 3' UTR sequence motifs that mediate negative post-transcriptional regulation.[J]. Nature Genetics, 2002, 30(4):363-364.

[4]. Karagiannis, P., Y. Fujita, and H. Saito.,RNA-based gene circuits for cell regulation. Proc Jpn Acad Ser B Phys Biol Sci, 2016. 92(9): p. 412-422.

[5]. Du, P., et al., Engineering Translational Activators with CRISPR-Cas System. ACS Synth Biol, 2016. 5(1): p. 74-80.

[6]. Green A, Silver P, Collins J, et al. Toehold switches: de-novo-designed regulators of gene expression.[J]. Cell, 2014, 159(4):925-939.





Contact Us : oucigem@163.com | ©2018 OUC IGEM.All Rights Reserved.
banner banner banner banner banner