Line 54: | Line 54: | ||
<li> | <li> | ||
<a data-target="#stdpolyacrilamidegeel">Standard polyacrylamide gel</a> | <a data-target="#stdpolyacrilamidegeel">Standard polyacrylamide gel</a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="#productpurification" data-toggle="collapse" aria-expanded="false" data-change="sidemenu"> | ||
+ | <span class="d-inline-bock open" data-change="el"></span> | ||
+ | Production/purification | ||
+ | </a> | ||
+ | <ul class="collapse list-unstyled" id="productpurification"> | ||
+ | <li> | ||
+ | <a data-target="#criofracture">Criofracture</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a data-target="#fluorescentproteinproduction">Fluorescent protein production</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a data-target="#proteinproductionpurificationprotocol">Protein production/purification | ||
+ | protocol</a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="#proteicquantification" data-toggle="collapse" aria-expanded="false" data-change="sidemenu"> | ||
+ | <span class="d-inline-bock open" data-change="el"></span> | ||
+ | Proteic quantification | ||
+ | </a> | ||
+ | <ul class="collapse list-unstyled" id="proteicquantification"> | ||
+ | <li> | ||
+ | <a data-target="#bradford">Bradford</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a data-target="#lowry">Lowry</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a data-target="#piercebca">Pierce BCA</a> | ||
</li> | </li> | ||
</ul> | </ul> | ||
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<div class="collapse" id="stdpolyacrilamidegeel"> | <div class="collapse" id="stdpolyacrilamidegeel"> | ||
<object type="application/pdf" data="https://static.igem.org/mediawiki/2018/2/2c/T--TecCEM--Experiments-Proteins5.pdf" | <object type="application/pdf" data="https://static.igem.org/mediawiki/2018/2/2c/T--TecCEM--Experiments-Proteins5.pdf" | ||
+ | width="100%" height="900px"> | ||
+ | </object> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <h2>Production/purification</h2> | ||
+ | <div class="row"> | ||
+ | <div class="col"> | ||
+ | <a class="btn-link text-notebook" data-toggle="collapse" href="#criofracture" role="button" | ||
+ | aria-expanded="false" aria-controls="criofracture"> | ||
+ | <h3>Criofracture</h3> | ||
+ | </a> | ||
+ | <div class="collapse" id="criofracture"> | ||
+ | <object type="application/pdf" data="https://static.igem.org/mediawiki/2018/7/79/T--TecCEM--Experiments-Proteins6.pdf" | ||
+ | width="100%" height="900px"> | ||
+ | </object> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="row"> | ||
+ | <div class="col"> | ||
+ | <a class="btn-link text-notebook" data-toggle="collapse" href="#fluorescentproteinproduction" role="button" | ||
+ | aria-expanded="false" aria-controls="fluorescentproteinproduction"> | ||
+ | <h3>Fluorescent protein production</h3> | ||
+ | </a> | ||
+ | <div class="collapse" id="fluorescentproteinproduction"> | ||
+ | <object type="application/pdf" data="https://static.igem.org/mediawiki/2018/7/74/T--TecCEM--Experiments-Proteins7.pdf" | ||
+ | width="100%" height="900px"> | ||
+ | </object> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="row"> | ||
+ | <div class="col"> | ||
+ | <a class="btn-link text-notebook" data-toggle="collapse" href="#proteinproductionpurificationprotocol" | ||
+ | role="button" aria-expanded="false" aria-controls="proteinproductionpurificationprotocol"> | ||
+ | <h3>Protein production/purification | ||
+ | protocol</h3> | ||
+ | </a> | ||
+ | <div class="collapse" id="proteinproductionpurificationprotocol"> | ||
+ | <object type="application/pdf" data="https://static.igem.org/mediawiki/2018/e/e9/T--TecCEM--Experiments-Proteins8.pdf" | ||
+ | width="100%" height="900px"> | ||
+ | </object> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <h2>Proteic quantification</h2> | ||
+ | <div class="row"> | ||
+ | <div class="col"> | ||
+ | <a class="btn-link text-notebook" data-toggle="collapse" href="#bradford" role="button" | ||
+ | aria-expanded="false" aria-controls="bradford"> | ||
+ | <h3>Bradford</h3> | ||
+ | </a> | ||
+ | <div class="collapse" id="bradford"> | ||
+ | <object type="application/pdf" data="https://static.igem.org/mediawiki/2018/f/f9/T--TecCEM--Experiments-Proteins9.pdf" | ||
+ | width="100%" height="900px"> | ||
+ | </object> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="row"> | ||
+ | <div class="col"> | ||
+ | <a class="btn-link text-notebook" data-toggle="collapse" href="#lowry" role="button" aria-expanded="false" | ||
+ | aria-controls="lowry"> | ||
+ | <h3>Lowry</h3> | ||
+ | </a> | ||
+ | <div class="collapse" id="lowry"> | ||
+ | <object type="application/pdf" data="https://static.igem.org/mediawiki/2018/9/90/T--TecCEM--Experiments-Proteins10.pdf" | ||
+ | width="100%" height="900px"> | ||
+ | </object> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="row"> | ||
+ | <div class="col"> | ||
+ | <a class="btn-link text-notebook" data-toggle="collapse" href="#piercebca" role="button" | ||
+ | aria-expanded="false" aria-controls="piercebca"> | ||
+ | <h3>Pierce BCA</h3> | ||
+ | </a> | ||
+ | <div class="collapse" id="piercebca"> | ||
+ | <object type="application/pdf" data="https://static.igem.org/mediawiki/2018/a/af/T--TecCEM--Experiments-Proteins11.pdf" | ||
width="100%" height="900px"> | width="100%" height="900px"> | ||
</object> | </object> |
Revision as of 21:51, 17 October 2018
Experiments
This is our experiment section. Here we compile important protocols for the development of TecTissue, ranging from our bacterial transformation procedures to our cell proliferation assays. We also address cell culture maintenance and protein loaded chitosan nanoparticles. Here you may find the protocol for our growth factor delivery to damaged cells and how much harm can be inflicted in vitro.