Difference between revisions of "Team:Kyoto/SpecialMethods"

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     <span class="box-title"><font face="Segoe UI">Table of contents</font></span>
 
     <span class="box-title"><font face="Segoe UI">Table of contents</font></span>
 
           <ul class="index1">
 
           <ul class="index1">
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             <li><a href="#Boil Method">1)Boil Method</a></li>
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             <li><a href="#Boil Method">1) Boil Method</a></li>
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             <li><a href="#"><font color="#fffafa"><font face="Segoe UI">2)</font></a></li>
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             <li><a href="#SLiCE">2) SLiCE</font></a></li>
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            <li><a href="#"><font color="#fffafa"><font face="Segoe UI">3)</font></a></li>
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          </ul>
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            <li><a href="#"><font color="#fffafa"><font face="Segoe UI">4)</font></a></li>
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</div>
 
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Revision as of 22:07, 17 October 2018

Team:Kyoto/Project - 2018.igem.org

Table of contents
1)Boil Method

1.Monitor OD of yeast and incubate until it becomes OD≒1.
2.Centrifuge yeast at 3500 rpm for 5 min
3.Discard the supernatant
4.Pipette the culture residual substance and transfer 1 ml of it to an Eppendorf tube
5.Centrifuge on FLASH and then decantate it
6.Add 1 ml of distilled water
7.Vortex
8.Centrifuge yeast at FLASH
9.Discard the supernatant (wash 1st time)
10.Repeat steps 6-9 (wash 2nd)
11.Repeat steps 6-9 (wash 3rd)
12.Cryopreservation
13.Add 1 ml of distilled water and vortex
14.Boil it with hot water for 10 min
· Fit the tube in the sponge and put it in boiling water
(· Keep the fire between low to medium heat)
15.Centrifuge at 10000 rpm for 5 min, and remove 900μl of the supernatant
16.Measure Na+ concentration(Atomic Absorption Spectrometry)


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2)

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3)

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4)


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