Difference between revisions of "Team:TecCEM/Experiments"
| Line 144: | Line 144: | ||
<ul class="collapse list-unstyled" id="celltransformation"> | <ul class="collapse list-unstyled" id="celltransformation"> | ||
<li> | <li> | ||
| − | <a data-target="# | + | <a data-target="#chemocompetentcells">Chemocompetent cells transformation protocol</a> |
| + | </li> | ||
| + | <li> | ||
| + | <a data-target="#pcc">Protocol for chemocompetent cells</a> | ||
| + | </li> | ||
| + | <li> | ||
| + | <a data-target="#tsc">Thermal Shock cell transformation protocol</a> | ||
</li> | </li> | ||
</ul> | </ul> | ||
| Line 155: | Line 161: | ||
<ul class="collapse list-unstyled" id="enzymerestriction"> | <ul class="collapse list-unstyled" id="enzymerestriction"> | ||
<li> | <li> | ||
| − | <a data-target="# | + | <a data-target="#ligacion">Protocol for enzyme digestion/alkaline phosphatase treatment of iGEM plasmids and ligation</a> |
| + | </li> | ||
| + | <li> | ||
| + | <a data-target="#enzymelig">Protocol for enzyme ligation of iGEM parts</a> | ||
| + | </li> | ||
| + | <li> | ||
| + | <a data-target="#enzres">Protocol for enzyme restriction of IDT parts and iGEM plasmid</a> | ||
</li> | </li> | ||
</ul> | </ul> | ||
| Line 166: | Line 178: | ||
<ul class="collapse list-unstyled" id="pcr"> | <ul class="collapse list-unstyled" id="pcr"> | ||
<li> | <li> | ||
| − | <a data-target="# | + | <a data-target="#protocolforcolonypcr">Protocol for Colony PCR</a> |
| + | </li> | ||
| + | <li> | ||
| + | <a data-target="#protocolforpcrvrvf2primers">Protocol for PCR using VR and VF2 primers</a> | ||
</li> | </li> | ||
</ul> | </ul> | ||
| Line 177: | Line 192: | ||
<ul class="collapse list-unstyled" id="plasmidextraction"> | <ul class="collapse list-unstyled" id="plasmidextraction"> | ||
<li> | <li> | ||
| − | <a data-target="# | + | <a data-target="#agarosebandpurification">Agarose Band purification (GenElute - Sigma)</a> |
| + | </li> | ||
| + | <li> | ||
| + | <a data-target="#alkalineplasmidpurification">Alkaline plasmid purification protocol</a> | ||
| + | </li> | ||
| + | <li> | ||
| + | <a data-target="#miniprepsigma">MiniPrep Sigma extraction protocol</a> | ||
</li> | </li> | ||
</ul> | </ul> | ||
Revision as of 22:37, 17 October 2018
Experiments
This is our experiment section. Here we compile important protocols for the development of TecTissue, ranging from our bacterial transformation procedures to our cell proliferation assays. We also address cell culture maintenance and protein loaded chitosan nanoparticles. Here you may find the protocol for our growth factor delivery to damaged cells and how much harm can be inflicted in vitro.