Difference between revisions of "Team:IIT Kanpur/InterLab"

 
(28 intermediate revisions by 2 users not shown)
Line 1: Line 1:
−
{{IIT_Kanpur}}
+
{{Template:IIT_Kanpur}}
 +
<div id="whiteBox" style="width: auto;  padding: 10px; height: auto; margin-left: 0px;
 +
    margin-right: 0px; position: absolute; top: 0px; left: 10%; right: 10%; z-index:-1;">
 
<html>
 
<html>
 +
<body>
 +
<h1>InterLab<br></h1>
 +
<p style="font-size:120%;">Complying with the trend of InterLab Study, iGEM conducts every year, to achieve the full potential of synthetic biology, invariably making every lab on earth build upon others’ work. <br>
 +
The question of iGEM 2018 InterLab Study was, ‘Can we reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of OD?’<br><br>
 +
Approach 1:<br>
 +
Converting absorbance of cells to absorbance of a known concentration of beads.<br>
 +
Approach 2:<br>
 +
Counting colony-forming units (CFUs) from the sample.<br><br>
 +
</p>
 +
</body>
 +
<body>
 +
<!-------------------------->
  
 +
<meta name="viewport" content="width=device-width, initial-scale=1">
 +
<head>
 +
<style>
 +
.collapsible {
 +
  background-color: #777;
 +
  color: white;
 +
  cursor: pointer;
 +
  padding: 18px;
 +
  width: 100%;
 +
  align: right;
 +
  border: none;
 +
  text-align: left;
 +
  outline: none;
 +
  font-size: 18px;
 +
}
  
 +
.active, .collapsible:hover {
 +
  background-color: #555;
 +
}
  
 +
.contentcollap {
 +
  padding: 0 18px;
 +
  max-height: 0;
 +
  overflow: hidden;
 +
  transition: max-height 0.2s ease-out;
 +
  background-color: #f1f1f1;
 +
}
 +
</style>
 +
</head>
 +
<body>
  
 +
<button class="collapsible">The Protocol</button>
 +
<div class="contentcollap">
 +
<embed src="https://static.igem.org/mediawiki/2018/0/09/2018_InterLab_Plate_Reader_Protocol.pdf" width="100%" height="400px" style="float:left"
 +
type='application/pdf'/>
 +
</div>
  
−
<div class="clear"></div>
+
<h3>Here are the summarised results of our interlab:<br></h3>
 +
<button class="collapsible">Particle standard curve (Microsphere protocol)
 +
</button>
 +
<div class="contentcollap">
 +
<img src = "https://static.igem.org/mediawiki/2018/f/f6/T--IIT_Kanpur--Interlab01.png" width="48%" style = "margin-left: auto;margin-right: auto;padding:0% 0% 0% 0%;border:5px solid black"></img>
 +
<img src = "https://static.igem.org/mediawiki/2018/3/3a/T--IIT_Kanpur--Interlab02.png" width="48%" style = "margin-left: auto;margin-right: auto;padding:0% 0% 0% 0%;border:5px solid black"></img>
 +
</div>
  
 +
<button class="collapsible">Fluorescein Standard Curve</button>
 +
<div class="contentcollap">
 +
<img src = "https://static.igem.org/mediawiki/2018/3/39/T--IIT_Kanpur--Interlab03.png" width="48%" style = "margin-left: auto;margin-right: auto;padding:0% 0% 0% 0%;border:5px solid black"></img>
 +
<img src = "https://static.igem.org/mediawiki/2018/c/ca/T--IIT_Kanpur--Interlab04.png" width="48%" style = "margin-left: auto;margin-right: auto;padding:0% 0% 0% 0%;border:5px solid black"></img>
 +
</div>
  
−
<div class="column full_size">
+
<button class="collapsible">Cell measurement protocol</button>
−
<h1>InterLab</h1>
+
<div class="contentcollap">
−
<h3>Bronze Medal Criterion #4</h3>
+
  <p style="font-size:120%;">
−
<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or obtain new, high quality experimental characterization data for an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2018 part number range.
+
The first column represents the fluorescence reading of two replicates for every device
−
<br><br>
+
The second column represents the OD reading of two replicates for every device.</p><br>
−
For teams participating in the <a href="https://2018.igem.org/Measurement/InterLab">InterLab study</a>, all work must be shown on this page.
+
<img src = "https://static.igem.org/mediawiki/2018/6/65/T--IIT_Kanpur--Interlab05.png" width="48%" style = "margin-left: auto;margin-right: auto;padding:0% 0% 0% 0%;border:5px solid black"></img>
−
 
+
<img src = "https://static.igem.org/mediawiki/2018/8/8e/T--IIT_Kanpur--Interlab06.png" width="48%" style = "margin-left: auto;margin-right: auto;padding:0% 0% 0% 0%;border:5px solid black"></img>
 +
<img src = "https://static.igem.org/mediawiki/2018/3/34/T--IIT_Kanpur--Interlab07.png" width="48%" style = "margin-left: auto;margin-right: auto;padding:0% 0% 0% 0%;border:5px solid black"></img>
 +
<img src = "https://static.igem.org/mediawiki/2018/6/66/T--IIT_Kanpur--Interlab08.png" width="48%" style = "margin-left: auto;margin-right: auto;padding:0% 0% 0% 0%;border:5px solid black"></img>
 +
<br>
 
</p>
 
</p>
−
</div>
 
−
 
−
 
  
 +
</div>
  
 +
<script>
 +
var coll = document.getElementsByClassName("collapsible");
 +
var i;
  
 +
for (i = 0; i < coll.length; i++) {
 +
  coll[i].addEventListener("click", function() {
 +
    this.classList.toggle("active");
 +
    var content = this.nextElementSibling;
 +
    if (content.style.maxHeight){
 +
      content.style.maxHeight = null;
 +
    } else {
 +
      content.style.maxHeight = content.scrollHeight + "px";
 +
    }
 +
  });
 +
}
 +
</script>
  
 +
</body>
 +
<!----------->
 +
</body>
 +
<body>
  
 +
</body>
 
</html>
 
</html>
 +
{{Template:IIT_Kanpur/Footer}}

Latest revision as of 22:45, 17 October 2018

InterLab

Complying with the trend of InterLab Study, iGEM conducts every year, to achieve the full potential of synthetic biology, invariably making every lab on earth build upon others’ work.
The question of iGEM 2018 InterLab Study was, ‘Can we reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of OD?’

Approach 1:
Converting absorbance of cells to absorbance of a known concentration of beads.
Approach 2:
Counting colony-forming units (CFUs) from the sample.

Here are the summarised results of our interlab:

The first column represents the fluorescence reading of two replicates for every device The second column represents the OD reading of two replicates for every device.