Difference between revisions of "Team:IIT Kanpur/InterLab"

 
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<h1>InterLab<br></h1>
 
<h1>InterLab<br></h1>
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<p style="font-size:120%;">Complying with the trend of InterLab Study, iGEM conducts every year, to achieve the full potential of synthetic biology, invariably making every lab on earth build upon others’ work. <br>
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The question of iGEM 2018 InterLab Study was, ‘Can we reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of OD?’<br><br>
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<p style="font-size:120;">Complying with the trend of InterLab Study, iGEM conducts every year, to achieve the full potential of synthetic biology, invariably making every lab on earth build upon others’ work. <br>
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The question of iGEM 2018 InterLab Study was, ‘Can we reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of OD?’<br>
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Approach 1:<br>
 
Approach 1:<br>
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Converting absorbance of cells to absorbance of a known concentration of beads.<br><br>
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Converting absorbance of cells to absorbance of a known concentration of beads.<br>
 
Approach 2:<br>
 
Approach 2:<br>
 
Counting colony-forming units (CFUs) from the sample.<br><br>
 
Counting colony-forming units (CFUs) from the sample.<br><br>
 
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<h3>Here are the summarised results of our interlab:<br></h3>
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<button class="collapsible">Particle standard curve (Microsphere protocol)
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<img src = "https://static.igem.org/mediawiki/2018/f/f6/T--IIT_Kanpur--Interlab01.png" width="48%" style = "margin-left: auto;margin-right: auto;padding:0% 0% 0% 0%;border:5px solid black"></img>
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<img src = "https://static.igem.org/mediawiki/2018/3/3a/T--IIT_Kanpur--Interlab02.png" width="48%" style = "margin-left: auto;margin-right: auto;padding:0% 0% 0% 0%;border:5px solid black"></img>
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The first column represents the fluorescence reading of two replicates for every device
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The second column represents the OD reading of two replicates for every device.</p><br>
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<img src = "https://static.igem.org/mediawiki/2018/6/65/T--IIT_Kanpur--Interlab05.png" width="48%" style = "margin-left: auto;margin-right: auto;padding:0% 0% 0% 0%;border:5px solid black"></img>
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<img src = "https://static.igem.org/mediawiki/2018/3/34/T--IIT_Kanpur--Interlab07.png" width="48%" style = "margin-left: auto;margin-right: auto;padding:0% 0% 0% 0%;border:5px solid black"></img>
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<img src = "https://static.igem.org/mediawiki/2018/6/66/T--IIT_Kanpur--Interlab08.png" width="48%" style = "margin-left: auto;margin-right: auto;padding:0% 0% 0% 0%;border:5px solid black"></img>
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Latest revision as of 22:45, 17 October 2018

InterLab

Complying with the trend of InterLab Study, iGEM conducts every year, to achieve the full potential of synthetic biology, invariably making every lab on earth build upon others’ work.
The question of iGEM 2018 InterLab Study was, ‘Can we reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of OD?’

Approach 1:
Converting absorbance of cells to absorbance of a known concentration of beads.
Approach 2:
Counting colony-forming units (CFUs) from the sample.

Here are the summarised results of our interlab:

The first column represents the fluorescence reading of two replicates for every device The second column represents the OD reading of two replicates for every device.