Difference between revisions of "Team:SHSBNU China/Hardware"

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<h6 id="menu_intro">Parts</h6>
 
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<a class="snd_class" href="https://2018.igem.org/Team:SHSBNU_China/Project">Project</a>
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<a class="snd_class" href="https://2018.igem.org/Team:SHSBNU_China/Parts#O">Parts Overview</a>
 
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Our team designed a water filter for sewage treatment named Filter. The filter bead filled with bi-odegradable materials polyhydroxyalkanoates (PHA) as a solid platform as well as carbon source for our Biofilm x Laccase, so it can decompose azo dyes while letting the liquid flow through the filter.
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        <p>We have finished constructing all the parts(<a href="http://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2018&group=SHSBNU_China">BBa_K2684000- BBa_K2684006</a>) which have all been sequenced and submitted to the iGEM parts registry</p>
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We find out that the Filter benefits the practical applications for several aspects. First, the  con-sumable filter bead is degradable and environmental friendly. Second, Biofilm x Laccase system accelerates the disposal by catalyzing azo dyes degradation in the concentrated filter bead, in turn, saving time and increasing the efficiency. Third, living bacteria in Biofilm x Laccase system sustain longer than other filtration materials such as activated carbon which is easily to wear off by time and needed to change frequently.
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<td color="#FFFFF0">Name</td>
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<td color="#FFFFF0">Description</td>
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<h3>a. Sample</h3>
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<td>BBa_K52684000</td>
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<td>CotA laccase of <em>B. subtilis</em></td>
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<td>CotA of <em>B. subtilis</em> is a copper-dependet laccase, which can catalyze the oxidation of board range of synthetic dyes. </td>
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<td>BBa_K52684001</td>
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<td>PelB-CotA</td>
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<td>PelB signal peptide linking with CotA to transfer CotA out of the bacteria. </td>
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<td>BBa_K52684002</td>
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<td>PhoA-CotA</td>
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<td>PhoA signal peptide linking with CotA to transfer CotA out of the bacteria. </td>
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<td>BBa_K52684003</td>
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<td>OmpA-CotA</td>
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<td>OmpA signal peptide linking with CotA to transfer CotA out of the bacteria.</td>
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<td>BBa_K52684004</td>
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<td>SpyCatcher-sfGFP</td>
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<td>SfGFP fused to the SpyCatcher domain can be covalently attached onto the biofilm displaying SpyTag.This part is used to test our immobilization strategy of CotA.</td>
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<td>BBa_K52684005</td>
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<td>SpyCatcher-CotA</td>
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<td>CotA fused to the SpyCatcher domain can be covalently attached onto the biofilm displaying SpyTag.</td>
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<td>BBa_K52684006</td>
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<td>CsgA-SpyTag</td>
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<td>CsgA is a major subunit of the biofilm of E. coli(1)(2). A SpyTag is fused to CsgA so that CotA laccase can be fixed on biofilm through SpyTag-SpyCatcher chemistry.</td>
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<h3 id="IPP">a. Improve Previous Parts</h3>
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<p>We improved part<a href="http://parts.igem.org/Part:BBa_K1583000">BBa_K1583000</a> by adding a sequence of SpyTag in order to fix CotA laccase onto the biofilm. </p>
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<p>We improved part<a href="http://parts.igem.org/Part:BBa_K1336002">BBa_K1336002</a> by eliminating its EcoRI cutting site. </p>
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(1). Wang, X, et al. “Programming Cells for Dynamic Assembly of Inorganic Nano-Objects with Spatiotemporal Control.” <em>Advanced Materials (Deerfield Beach, Fla.).,</em> U.S. National Library of Medicine, Apr. 2018, www.ncbi.nlm.nih.gov/pubmed/29516606.
 
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(2). Chen, Allen Y., et al. “Synthesis and Patterning of Tunable Multiscale Materials with Engineered Cells .” <em>Nature Materials,</em> U.S. National Library of Medicine, May 2014, www.ncbi.nlm.nih.gov/pmc/articles/PMC4063449/.
 
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Revision as of 22:55, 17 October 2018

Parts

Parts

I. Overview

We have finished constructing all the parts(BBa_K2684000- BBa_K2684006) which have all been sequenced and submitted to the iGEM parts registry

Name Description Function
BBa_K52684000 CotA laccase of B. subtilis CotA of B. subtilis is a copper-dependet laccase, which can catalyze the oxidation of board range of synthetic dyes.
BBa_K52684001 PelB-CotA PelB signal peptide linking with CotA to transfer CotA out of the bacteria.
BBa_K52684002 PhoA-CotA PhoA signal peptide linking with CotA to transfer CotA out of the bacteria.
BBa_K52684003 OmpA-CotA OmpA signal peptide linking with CotA to transfer CotA out of the bacteria.
BBa_K52684004 SpyCatcher-sfGFP SfGFP fused to the SpyCatcher domain can be covalently attached onto the biofilm displaying SpyTag.This part is used to test our immobilization strategy of CotA.
BBa_K52684005 SpyCatcher-CotA CotA fused to the SpyCatcher domain can be covalently attached onto the biofilm displaying SpyTag.
BBa_K52684006 CsgA-SpyTag CsgA is a major subunit of the biofilm of E. coli(1)(2). A SpyTag is fused to CsgA so that CotA laccase can be fixed on biofilm through SpyTag-SpyCatcher chemistry.

a. Improve Previous Parts

We improved partBBa_K1583000 by adding a sequence of SpyTag in order to fix CotA laccase onto the biofilm.

We improved partBBa_K1336002 by eliminating its EcoRI cutting site.

II. References

(1). Wang, X, et al. “Programming Cells for Dynamic Assembly of Inorganic Nano-Objects with Spatiotemporal Control.” Advanced Materials (Deerfield Beach, Fla.)., U.S. National Library of Medicine, Apr. 2018, www.ncbi.nlm.nih.gov/pubmed/29516606.

(2). Chen, Allen Y., et al. “Synthesis and Patterning of Tunable Multiscale Materials with Engineered Cells .” Nature Materials, U.S. National Library of Medicine, May 2014, www.ncbi.nlm.nih.gov/pmc/articles/PMC4063449/.