Difference between revisions of "Team:HUST-China/Notebook"

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<h1>Notebook</h1>
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th{
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<p> Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.</p>
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                      <li class="HillSide"><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China">Home</a></li>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/project/background">Background</a></li>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Description">Description</a></li>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Experiments">Experiments</a></li>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/wetlab/protocols">Protocols</a></li>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/InterLab">Interlab</a></li>
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                          <a href="#" data-toggle="dropdown" class="dropdown-toggle waves-effect waves-dark">Model<b class="caret"></b></a>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Modeling overview">Modeling overview</a></li>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/model_of_systems">Model of systems</a></li>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Sort of three genes">Sort of three genes</a></li>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Parts Overview">Parts Overview</a></li>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Composite part">Composite part</a></li>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Part collection">Part collection</a></li>
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                          <a href="#" data-toggle="dropdown" class="dropdown-toggle waves-effect waves-dark">HP<b class="caret"></b></a>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Education_Engagement">Education&Engagement</a></li>
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                          </ul>
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                          <a href="#" data-toggle="dropdown" class="dropdown-toggle waves-effect waves-dark">TEAM<b class="caret"></b></a>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Team">Team Members</a></li>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Collaborations">Collaborations</a></li>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Attributions">Attributions</a></li>
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                          </ul>
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                      </li>
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                    </ul>
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                </div>
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  <div class="container">
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      <div class="col-lg-12">
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        <h2 class="pageTitle">NoteBook</h2>
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      </div>
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    </div>
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  </div>
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  </section>
  
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<div class="column two_thirds_size">
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  <section class="content">
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<h3>What should this page have?</h3>
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  <div class="container">
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<ul>
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        <div class="row">  
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<li>Chronological notes of what your team is doing.</li>
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            <div class="col-md-12">  
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<li> Brief descriptions of daily important events.</li>
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              <!-- Accordion starts -->
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<li>Pictures of your progress. </li>
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              <ul class="collapsible" id="accordionSection" data-collapsible="accordion">
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<li>Mention who participated in what task.</li>
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                <li>
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</ul>
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                  <div class="collapsible-header HillSide"><i><img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/89/T--HUST-China--2018-coin04.png"></i>April 1-30</div>
 +
                  <div class="collapsible-body"><p><span class="red-content">Ziyang Xiao, Junhao Xiong, Jingyu Ren, Yan chen, Qian Li</span><br>Pre-experiment of electrogenesis: tested and optimized the electrogenesis condition for MFC (Microbial Fuel Cell)</p></div>
 +
                </li>
  
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<div class="column third_size">
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                <li>
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<div class="highlight decoration_A_full">
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                  <div class="collapsible-header"><i><img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/89/T--HUST-China--2018-coin04.png"></i>May 1-31</div>
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<h3>Inspiration</h3>
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                  <div class="collapsible-body"><p><span class="red-content">Yiyan Yu, Ziyi Li, Junhao Xiong</span><br>
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<p>You can see what others teams have done to organize their notes:</p>
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                    Basic design of molecular experiments: design sequences, primers and the construction of Shewanella
 +
                  </p></div>
 +
                </li>
  
−
<ul>  
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                <li>
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<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
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                  <div class="collapsible-header"><i><img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/89/T--HUST-China--2018-coin04.png"></i>July 1-7</div>
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<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
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                  <div class="collapsible-body"><p><span class="red-content">Junhao Xiong, Ziqi Yin, Jiayi Liu</span><br>
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<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
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                    Preparation for following experiments including preparing LB culture medium, antibiotics and optimizing culture condition of Shewanella oneidensis MR-1.<br>
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<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
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                    <p><span class="red-content">Bo Peng</span><br>
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</ul>
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                    Construction of pSB1C3-RBS-mleS
−
</div>
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                    </p>
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</div>
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                    <div class="col-md-4 col-md-offset-1">
 +
                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/7/77/T--HUST-China--2018-notebook01.png">
 +
                    </div>
 +
                    <div class="col-md-12">
 +
                      <p><span class="red-content">Ziyang Xiao, Haotian Ren, Yan Chen, Hao Qiu</span><br>
 +
                      synechocystis Group took the responsibility of doing the Interlab. Preparation for the experiment.
 +
                      </p>
 +
                    </div>
 +
                  </div>
 +
                </li>
  
 +
 +
                <li>
 +
                  <div class="collapsible-header"><i><img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/89/T--HUST-China--2018-coin04.png"></i>July 8-21</div>
 +
                  <div class="collapsible-body"><p><span class="red-content">Junhao Xiong, Ziqi Yin, Jiayi Liu</span><br>
 +
                  Construction of pYYDT-pflB: PCR, double enzyme digestion (with NdeI & SalI), ligation, transformation (into E.coli TOP10).
 +
                  </p>
 +
                    <div class="col-md-6 col-md-offset-1">
 +
                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/1/12/T--HUST-China--2018-notebook02.png">
 +
                    </div>
 +
                    <div class="col-md-12">
 +
                      <p><span class="red-content">Qian Li</span><br>
 +
                        Construction of pSB1C3-RBS-lldp-RBS-ldhA-TT
 +
                      </p>
 +
                    </div>
 +
                    <div class="col-md-4 col-md-offset-1">
 +
                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/2/2b/T--HUST-China--2018-notebook03.png">
 +
                    </div>
 +
                    <div class="col-md-12">
 +
                      <p><span class="red-content">Ziyang Xiao, Haotian Ren, Yan Chen, Hao Qiu</span><br>
 +
                        Finishing the cell cultures cultivation and using some machines to measure some related data.
 +
                      </p>
 +
                    </div>
 +
                  </div>
 +
                </li>
 +
 +
 +
                <li>
 +
                  <div class="collapsible-header"><i><img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/89/T--HUST-China--2018-coin04.png"></i>July 22-29</div>
 +
                  <div class="collapsible-body"><p><span class="red-content">Junhao Xiong, Ziqi Yin, Jiayi Liu</span><br>
 +
                  Construction of pYYDT-gapA-mdh, pYYDT-lldEFG: PCR, double enzyme digestion (with NdeI & SalI), ligation, transformation (into E.coli TOP10).
 +
                  </p>
 +
                    <div class="col-md-6 col-md-offset-1">
 +
                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/3/37/T--HUST-China--2018-notebook04.png">
 +
                    </div>
 +
                    <div class="col-md-12">
 +
                      <p><span class="red-content">Haibo Huang</span><br>
 +
                        Construction of pSB1C3-RBS-lldP and pSB1C3-RBS-ldhA.
 +
                      </p>
 +
                    </div>
 +
                    <div class="col-md-4 col-md-offset-1">
 +
                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/a/a3/T--HUST-China--2018-notebook05.png">
 +
                    </div>
 +
                    <div class="col-md-12">
 +
                      <p><span class="red-content">Haotian Ren</span><br>
 +
                        Restriction enzyme digestion of psb1c3<br>
 +
                        <span class="red-content">Ziyang Xiao, Yan Chen, Haotian Ren</span><br>
 +
                        Testing the cultivation ways of synechocystis PCC6803
 +
                      </p>
 +
                    </div>
 +
                  </div>
 +
                </li>
 +
                <li>
 +
                  <div class="collapsible-header"><i><img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/89/T--HUST-China--2018-coin04.png"></i>July 30 - August 5</div>
 +
                  <div class="collapsible-body">
 +
                    <p><span class="red-content">Ziyi Li, Jiayi Liu, Yizhe Zeng</span><br>
 +
                    Construction of pYYDT-gapA, pYYDT-mdh<br>
 +
                    Standardization of gapA, mdh
 +
                    </p>
 +
                  <div class="col-md-12">
 +
                  <div class="col-md-6 col-md-offset-1">
 +
                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/e/e6/T--HUST-China--2018-notebook06.png">
 +
                    </div>
 +
                  </div>
 +
                  <div class="col-md-12">
 +
                  <div class="col-md-6 col-md-offset-1">
 +
                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/0/08/T--HUST-China--2018-notebook07.png">
 +
                    </div>
 +
                  </div>
 +
                  <div class="col-md-12">
 +
                  <div class="col-md-6 col-md-offset-1">
 +
                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/8c/T--HUST-China--2018-notebook08.png">
 +
                    </div>
 +
                  </div>
 +
                  <div class="col-md-12">
 +
                      <p><span class="red-content">Kaiwen Liu</span><br>
 +
                        Construction of pmg105-PpckA-RBS-lldP-RBS-ldhA-TT
 +
                      </p>
 +
                  </div>
 +
                  <div class="col-md-12">
 +
                  <div class="col-md-4 col-md-offset-1">
 +
                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/4/4a/T--HUST-China--2018-notebook09.png">
 +
                    </div>
 +
                  </div>
 +
                  <div class="col-md-12">
 +
                      <p><span class="red-content">Yan Chen, Sijie Xu</span><br>
 +
                        Interlab Experiment 4
 +
                      </p>
 +
                  </div>
 +
                </div>
 +
                </li>
 +
 +
 +
 +
                <li>
 +
                  <div class="collapsible-header"><i><img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/89/T--HUST-China--2018-coin04.png"></i>August 6-12</div>
 +
                  <div class="collapsible-body"><p><span class="red-content">Ziyi Li, Jiayi Liu, Yizhe Zeng</span><br>
 +
                  Construction of pYYDT-dld<br>
 +
                  Standardization of dld, lldEFG
 +
                  </p>
 +
                    <div class="col-md-6 col-md-offset-1">
 +
                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/2/20/T--HUST-China--2018-notebook10.png">
 +
                    </div>
 +
                    <div class="col-md-12">
 +
                      <p><span class="red-content">Haibo Huang</span><br>
 +
                        Construction of pSB1C3-RBS-ldhA-RBS-lldP-TT
 +
                      </p>
 +
                    </div>
 +
                    <div class="col-md-6 col-md-offset-1">
 +
                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/b/b2/T--HUST-China--2018-notebook11.png">
 +
                    </div>
 +
                    <div class="col-md-12">
 +
                      <p><span class="red-content">Yan Chen, Yuanda Huang</span><br>
 +
                        Restriction enzyme digestion of psb-lldEFG <br>
 +
                        <span class="red-content">Yan Chen, Sijie Xu, Yuanda Huang, Ziyang Xiao</span><br>
 +
                        Constructing ldhDc-lldP, ldhDARSDR-lldP, ldhDnARSDR-lldPT
 +
                      </p>
 +
                    </div>
 +
                  </div>
 +
                </li>
 +
 +
 +
                <li>
 +
                  <div class="collapsible-header"><i><img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/89/T--HUST-China--2018-coin04.png"></i>August 13-19</div>
 +
                  <div class="collapsible-body"><p><span class="red-content">Ziyi Li, Yizhe Zeng</span><br>
 +
                  Overlap extension PCR of dld and lldEFG: We got the right band but the sequencing result revealed a mutation of it.
 +
                  </p>
 +
                    <div class="col-md-6 col-md-offset-1">
 +
                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/9/91/T--HUST-China--2018-notebook12.png">
 +
                    </div>
 +
                    <div class="col-md-12">
 +
                      <p>Making competent cells of E.coli WM3064: we need the deficiency type WM3064 to conjugate with Shewanella oneidensis MR-1.<br>
 +
                      <span class="red-content">Bo Peng</span><br>
 +
                      Construction of pmg105-PpckA-RBS-lldP-TT, pmg105-PpckA-RBS-ldhA-RBS-lldP-TT and pmg105-PpckA-RBS-mleS-TT.
 +
                      </p>
 +
                    </div>
 +
                    <div class="col-md-6 col-md-offset-1">
 +
                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/0/0e/T--HUST-China--2018-notebook13.png">
 +
                    </div>
 +
                    <div class="col-md-12">
 +
                      <p><span class="red-content">Yuanda Huang, Yan Chen, Sijie Xu, Tao Jian, Ziyang Xiao</span><br>
 +
                        PCR of ldhDnARSdR, PCR of ldhDARSdR,<br>
 +
                        Overlap extension PCR of ldhDnARSdR and lldp (Dn-L) <br>
 +
                        Overlap extension PCR of ldhDARSdR and lldp (DA-L)<br>
 +
                        Overlap extension PCR of ldhDC and lldp (DC-L)
 +
                      </p>
 +
                    </div>
 +
                  </div>
 +
                </li>
 +
 +
                <li>
 +
                  <div class="collapsible-header"><i><img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/89/T--HUST-China--2018-coin04.png"></i>August 20-26</div>
 +
                  <div class="collapsible-body"><p><span class="red-content">Ziyi Li, Junhao Xiong, Yizhe Zeng </span><br>
 +
                  Conjugation of E.coli WM3064 and Shewanella oneidensis MR-1: we transferred the targeted plasmid into E.coli WM3064 and conjugated WM3064 and Shewanella oneidensis MR-1 so that the Shewanella oneidensis MR-1 could finally get the targetted plasmid.<br>
 +
                    Conjugation pYYDT and pYYDT-lldEFG this week.
 +
                  </p>
 +
                    <div class="col-md-6 col-md-offset-1">
 +
                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/f/f4/T--HUST-China--2018-notebook14.png">
 +
                    </div>
 +
                    <div class="col-md-12">
 +
                      <p>Using device to produce electricity with Shewanella. We proved that Shewanella could produce more electricity with the existence of lactate.
 +
                      </p>
 +
                    </div>
 +
                    <div class="col-md-6 col-md-offset-1">
 +
                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/4/4a/T--HUST-China--2018-notebook15.png">
 +
                    </div>
 +
                    <div class="col-md-12">
 +
                      <p><span class="red-content">Yuanda Huang, Haotian Ren, Ziyang Xiao</span><br>
 +
                        PCR of ldhDnARSdR, PCR of ldhDARSdR,<br>
 +
                        PCR of ldhDnARSdR, extraction enzyme digestion of ldhDnARSdR,<br>
 +
                        Ligation of ldhDnARSdR and psb1c3, transformation<br>
 +
                        <span class="red-content">Hao Qiu</span><br>
 +
                        Constructing the pathway of TH-glad-lldp.<br>
 +
                        Insert sequence TH-glad-lldp to vector psb1c3.
 +
                      </p>
 +
                    </div>
 +
                  </div>
 +
                </li>
 +
 +
 +
                <li>
 +
                  <div class="collapsible-header"><i><img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/89/T--HUST-China--2018-coin04.png"></i>August 27 – September 2</div>
 +
                  <div class="collapsible-body"><p><span class="red-content">Ziyi Li, Junhao Xiong, Yizhe Zeng</span><br>
 +
                  Redesigning about connecting dld and lldEFG: We decided to drop the idea about overlap extension PCR to connect dld and lldEFG and started to use isocaudamer (XbaI and SpeI) to connect these genes.<br>
 +
                  </p>
 +
                    <div class="col-md-6 col-md-offset-1">
 +
                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/7/7a/T--HUST-China--2018-notebook16.png">
 +
                    </div>
 +
                    <div class="col-md-12">
 +
                      <p>Conjugation of pYYDT-gapA and pYYDT-mdh.<br>
 +
                        By constructing Rhodopseudomonas palustris-Shewanella oneidensis mutualism system, we found that Shewanella could produce more electricity if Rhodopseudomonas palustris exists.<br>
 +
                        <span class="red-content">Qian Li</span><br>
 +
                        Construction of pSB1C3-RBS-mleS-RBS-lldp.
 +
                      </p>
 +
                    </div>
 +
                    <div class="col-md-6 col-md-offset-1">
 +
                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/c/c0/T--HUST-China--2018-notebook17.png">
 +
                    </div>
 +
                    <div class="col-md-12">
 +
                      <p><span class="red-content">Yuanda Huang, Ziyang Xiao</span><br>
 +
                        Overlap extension PCR of ldhDnARSdR-lldp
 +
                      </p>
 +
                    </div>
 +
                    <div class="col-md-6 col-md-offset-1">
 +
                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/e/e2/T--HUST-China--2018-notebook18.png">
 +
                    </div>
 +
                    <div class="col-md-12">
 +
                      <p><span class="red-content">Tao Jian</span><br>
 +
                        Adding Flag tag to ldhD-lldP.<br>
 +
                        <span class="red-content">Yan Chen</span><br>
 +
                        Adding Flag tag to ldhDc-lldP
 +
                      </p>
 +
                    </div>
 +
 +
                  </div>
 +
                </li>
 +
 +
                <li>
 +
                  <div class="collapsible-header"><i><img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/89/T--HUST-China--2018-coin04.png"></i>September 3-9</div>
 +
                  <div class="collapsible-body"><p><span class="red-content">Ziyi Li, Junhao Xiong, Yizhe Zeng, Ziqi Yin</span><br>
 +
                  Conjugation of pYYDT-dld and pYYDT-Ptac-pflB.
 +
                  </p>
 +
                    <div class="col-md-6 col-md-offset-1">
 +
                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/1/1a/T--HUST-China--2018-notebook19.png">
 +
                    </div>
 +
                    <div class="col-md-12">
 +
                      <p>New method to connect dld and lldEFG. However, we failed at the construction of pSB1C3-ptac-lldEFG because of the biobrick prefix on pYYDT.<br>
 +
                      We proved that Shewanella could produce more electricty under anaerobic condition.<br>
 +
                      <span class="red-content">Yuanda Huang</span><br>
 +
                        Adding tags to ldhDnARSdR-lldp<br>
 +
                        <span class="red-content">Yan Chen</span><br>
 +
                      Adding his tag to ldhD-lldP and ldhDc-lldP<br>
 +
                      <span class="red-content">Hao Qiu</span><br>
 +
                      Amplifying the gene and transforming in E. coli.<br>
 +
                      <span class="red-content">Ziyang Xiao</span><br>
 +
                      synechocystis culture.
 +
                      </p>
 +
                    </div>
 +
                  </div>
 +
                </li>
 +
 +
                <li>
 +
                  <div class="collapsible-header"><i><img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/89/T--HUST-China--2018-coin04.png"></i>September 10-16</div>
 +
                  <div class="collapsible-body"><p><span class="red-content">Ziyi Li, Junhao Xiong, Yizhe Zeng, Ziqi Yin</span><br>
 +
                  Conjugation of pYYDT-gapA-mdh
 +
                  </p>
 +
                    <div class="col-md-6 col-md-offset-1">
 +
                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/9/9f/T--HUST-China--2018-notebook20.png">
 +
                    </div>
 +
                    <div class="col-md-12">
 +
                      <p>
 +
                      <span class="red-content">Haibo Huang</span><br>
 +
                        Adding tags to ldhDnARSdR-lldp<br>
 +
                        <span class="red-content">Yan Chen</span><br>
 +
                      Construction of pmg105-PpckA-RBS-ldhA-TT.
 +
                      </p>
 +
                    </div>
 +
                    <div class="col-md-6 col-md-offset-1">
 +
                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/e/e2/T--HUST-China--2018-notebook21.png">
 +
                    </div>
 +
                    <div class="col-md-12">
 +
                      <p>
 +
                      <span class="red-content">Yuanda Huang</span><br>
 +
                        Adding tags to ldhDARSdR-lldp<br>
 +
                        <span class="red-content">Yan Chen</span><br>
 +
                      Recombination of ldhD-lldP and ldhDc-lldP to shuttle plasmid PCK306<br>
 +
                      <span class="red-content">Hao Qiu</span><br>
 +
                      Checking the finial consequence by gene sequencing.<br>
 +
                      <span class="red-content">Ziyang Xiao</span><br>
 +
                      synechocystis culture.
 +
                      </p>
 +
                    </div>
 +
                  </div>
 +
                </li>
 +
 +
 +
                <li>
 +
                  <div class="collapsible-header"><i><img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/89/T--HUST-China--2018-coin04.png"></i>September 17-23</div>
 +
                  <div class="collapsible-body">
 +
                  <p><span class="red-content">Ziyi Li, Junhao Xiong, Yizhe Zeng, Ziqi Yin</span><br>
 +
                  Application of the electrogenesis: we used the device to charge an LED (1.7V), a cell phone (5V) and a small engine (5V). The success proved that we can put our device into practice.<br>
 +
                  We proved that carbon cloth has better effect than carbon rods.<br>
 +
                  <span class="red-content">Kaiwen Liu</span><br>
 +
                    Construction of pmg105-PpckA-RBS-mleS-RBS-lldP-TT
 +
                  </p>
 +
                    <div class="col-md-4 col-md-offset-1">
 +
                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/3/39/T--HUST-China--2018-notebook22.png">
 +
                    </div>
 +
                    <div class="col-md-12">
 +
                      <p><span class="red-content">Haibo Huang</span><br>
 +
                        Inserting ldhDnARSdR-lldp biaoda into the plasmid called pck306 through recombinase,<br>
 +
                        Inserting ldhDARSdR-lldp biaoda into the plasmid called pck306 through recombinase
 +
                      </p>
 +
                    </div>
 +
                    <div class="col-md-6 col-md-offset-1">
 +
                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/1/1f/T--HUST-China--2018-notebook23.png">
 +
                    </div>
 +
                    <div class="col-md-12">
 +
                      <p>
 +
                      <span class="red-content">Yan Chen</span><br>
 +
                        Transforming recombinant shuttle plasmid into synechocystis<br>
 +
                        <span class="red-content">Hao Qiu</span><br>
 +
                        Transforming ldhd sequence into synechocystis.<br>
 +
                      <span class="red-content">Ziyang Xiao</span><br>
 +
                        synechocystis culture.
 +
                      </p>
 +
                    </div>
 +
                  </div>
 +
                </li>
 +
 +
                <li>
 +
                  <div class="collapsible-header"><i><img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/89/T--HUST-China--2018-coin04.png"></i>September 24-30</div>
 +
                  <div class="collapsible-body">
 +
                  <p><span class="red-content">Ziyi Li, Junhao Xiong, Yizhe Zeng, Ziqi Yin</span><br>
 +
                    Construction of pSB1C3-pflB-fdh:we found that there was no tac promotor before.
 +
                  </p>
 +
                    <div class="col-md-6 col-md-offset-1">
 +
                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/b/bd/T--HUST-China--2018-notebook24.png">
 +
                    </div>
 +
                    <div class="col-md-12">
 +
                      <p>We proved that the electricity produced by Rhodopseudomonas palustrls could be ignored in the electrogenesis system.<br>
 +
                      At the same time, it was demonstrated that the lactate could be the only carbon source in the culture medium.
 +
                      <br>
 +
                      <span class="red-content">Qian Li</span><br>
 +
                      Construction of pSB1C3- RBS-mleS-RBS-lldP-RBS-ldhA-TT
 +
                      </p>
 +
                    </div>
 +
                    <div class="col-md-6 col-md-offset-1">
 +
                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/1/1a/T--HUST-China--2018-notebook25.png">
 +
                    </div>
 +
                    <div class="col-md-12">
 +
                      <p>
 +
                      <span class="red-content">Hao Qiu</span><br>
 +
                        Using DNS to detect the condition of producing lactate by synechocystis.<br>
 +
                      <span class="red-content">Yuanda Huang, Yan Chen</span><br>
 +
                        Transforming recombinant shuttle plasmid into synechocystis.<br>
 +
                      <span class="red-content">Ziyang Xiao</span><br>
 +
                        synechocystis culture.
 +
                      </p>
 +
                    </div>
 +
                  </div>
 +
                </li>
 +
 +
 +
                <li>
 +
                  <div class="collapsible-header"><i><img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/89/T--HUST-China--2018-coin04.png"></i>October 1-7</div>
 +
                  <div class="collapsible-body">
 +
                  <p><span class="red-content">Ziyi Li, Junhao Xiong, Yizhe Zeng, Ziqi Yin</span><br>
 +
                  Construction of pYYDT-pflB-fdh
 +
                  </p>
 +
                    <div class="col-md-6 col-md-offset-1">
 +
                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/5/5c/T--HUST-China--2018-notebook26.png">
 +
                    </div>
 +
                    <div class="col-md-12">
 +
                      <p>Conjugation of pYYDT-pflB-fdh<br>
 +
                          We proved that the engineering bacteria with pYYDT-gapA-mdh could produce more electricity than the bacteria with pYYDT.<br>
 +
                      <span class="red-content">Bo Peng</span><br>
 +
                      Construction of RBS-mleS-RBS-lldP-TT pmg105-PpckA-RBS-mleS-RBS-lldP-RBS-ldhA-tt.
 +
                      </p>
 +
                    </div>
 +
                    <div class="col-md-6 col-md-offset-1">
 +
                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/5/57/T--HUST-China--2018-notebook27.png">
 +
                    </div>
 +
                    <div class="col-md-12">
 +
                      <p>
 +
                      <span class="red-content">Yuanda Huang</span><br>
 +
                        Overlap extension PCR of ldhDnARSdR and lldp. <br>
 +
                      <span class="red-content">Hao Qiu</span><br>
 +
                        Expansion culture of synechocystis. DNS detection of sugar production of synechocystis, YFP fluorescent protein detection of synechocystis.<br>
 +
                      <span class="red-content">Yan Chen</span><br>
 +
                      Transforming recombinant shuttle plasmid into synechocystis.
 +
                      </p>
 +
                    </div>
 +
                  </div>
 +
                </li>
 +
 +
 +
                <li>
 +
                  <div class="collapsible-header"><i><img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/89/T--HUST-China--2018-coin04.png"></i>October 8-14</div>
 +
                  <div class="collapsible-body">
 +
                  <p><span class="red-content">Ziyi Li, Junhao Xiong, Yizhe Zeng, Ziqi Yin</span><br>
 +
                  1. Construction of pSB1C3-Ptac-pflB-fdh<br>
 +
                  2. Reconjugation of pYYDT-lldEFG<br>
 +
                  3. We did two groups of electrogenesis experiments. One of them included the engineering synechocystis and Shewanella, the other included the engineering Rhodopseudomonas palustris and Shewanella. It turned out that the later one worked better than the former one.<br>
 +
                      <span class="red-content">Yuanda Huang</span><br>
 +
                        Add the first tag to ldhDnARSdR-lldp /ldhDARSdR-lldp. <br>
 +
                      <span class="red-content">Hao Qiu</span><br>
 +
                      Detection of YFP fluorescent protein of synechocystis.<br>
 +
                      <span class="red-content">Yan Chen</span><br>
 +
                      Detection of YFP fluorescent protein of synechocystis. Detection of Flag tag.<br>
 +
                      <span class="red-content">Ziyang Xiao</span><br>
 +
                      Detection of YFP fluorescent protein of synechocystis. Detection of 6× His tag and lactate.<br>
 +
                      Conjugation of pYYDT-pflB-fdh<br>
 +
                      We proved that the engineering bacteria with pYYDT-gapA-mdh could produce more electricity than the bacteria with pYYDT.<br>
 +
                      <span class="red-content">Yuanda Huang</span><br>
 +
                        Overlap extension PCR of ldhDnARSdR and lldp. <br>
 +
                      <span class="red-content">Hao Qiu</span><br>
 +
                        Expansion culture of synechocystis. DNS detection of sugar production of synechocystis, YFP fluorescent protein detection of synechocystis.<br>
 +
                      <span class="red-content">Yan Chen</span><br>
 +
                      Transforming recombinant shuttle plasmid into synechocystis.
 +
                      </p>
 +
                    </div>
 +
                  </div>
 +
                </li>
 +
              </ul>
 +
                          <!-- Accordion ends -->
 +
             
 +
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                College of Life Science & Technology<br>
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                Huazhong University of Science and Technology<br>
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                Add: 1037 Luoyu Road, Wuhan, Hubei, China; P.C: 430074<br>
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Latest revision as of 23:02, 17 October 2018

iGEM 2018 HUST-China

NoteBook

  • April 1-30

    Ziyang Xiao, Junhao Xiong, Jingyu Ren, Yan chen, Qian Li
    Pre-experiment of electrogenesis: tested and optimized the electrogenesis condition for MFC (Microbial Fuel Cell)

  • May 1-31

    Yiyan Yu, Ziyi Li, Junhao Xiong
    Basic design of molecular experiments: design sequences, primers and the construction of Shewanella

  • July 1-7

    Junhao Xiong, Ziqi Yin, Jiayi Liu
    Preparation for following experiments including preparing LB culture medium, antibiotics and optimizing culture condition of Shewanella oneidensis MR-1.

    Bo Peng
    Construction of pSB1C3-RBS-mleS

    Ziyang Xiao, Haotian Ren, Yan Chen, Hao Qiu
    synechocystis Group took the responsibility of doing the Interlab. Preparation for the experiment.

  • July 8-21

    Junhao Xiong, Ziqi Yin, Jiayi Liu
    Construction of pYYDT-pflB: PCR, double enzyme digestion (with NdeI & SalI), ligation, transformation (into E.coli TOP10).

    Qian Li
    Construction of pSB1C3-RBS-lldp-RBS-ldhA-TT

    Ziyang Xiao, Haotian Ren, Yan Chen, Hao Qiu
    Finishing the cell cultures cultivation and using some machines to measure some related data.

  • July 22-29

    Junhao Xiong, Ziqi Yin, Jiayi Liu
    Construction of pYYDT-gapA-mdh, pYYDT-lldEFG: PCR, double enzyme digestion (with NdeI & SalI), ligation, transformation (into E.coli TOP10).

    Haibo Huang
    Construction of pSB1C3-RBS-lldP and pSB1C3-RBS-ldhA.

    Haotian Ren
    Restriction enzyme digestion of psb1c3
    Ziyang Xiao, Yan Chen, Haotian Ren
    Testing the cultivation ways of synechocystis PCC6803

  • July 30 - August 5

    Ziyi Li, Jiayi Liu, Yizhe Zeng
    Construction of pYYDT-gapA, pYYDT-mdh
    Standardization of gapA, mdh

    Kaiwen Liu
    Construction of pmg105-PpckA-RBS-lldP-RBS-ldhA-TT

    Yan Chen, Sijie Xu
    Interlab Experiment 4

  • August 6-12

    Ziyi Li, Jiayi Liu, Yizhe Zeng
    Construction of pYYDT-dld
    Standardization of dld, lldEFG

    Haibo Huang
    Construction of pSB1C3-RBS-ldhA-RBS-lldP-TT

    Yan Chen, Yuanda Huang
    Restriction enzyme digestion of psb-lldEFG
    Yan Chen, Sijie Xu, Yuanda Huang, Ziyang Xiao
    Constructing ldhDc-lldP, ldhDARSDR-lldP, ldhDnARSDR-lldPT

  • August 13-19

    Ziyi Li, Yizhe Zeng
    Overlap extension PCR of dld and lldEFG: We got the right band but the sequencing result revealed a mutation of it.

    Making competent cells of E.coli WM3064: we need the deficiency type WM3064 to conjugate with Shewanella oneidensis MR-1.
    Bo Peng
    Construction of pmg105-PpckA-RBS-lldP-TT, pmg105-PpckA-RBS-ldhA-RBS-lldP-TT and pmg105-PpckA-RBS-mleS-TT.

    Yuanda Huang, Yan Chen, Sijie Xu, Tao Jian, Ziyang Xiao
    PCR of ldhDnARSdR, PCR of ldhDARSdR,
    Overlap extension PCR of ldhDnARSdR and lldp (Dn-L)
    Overlap extension PCR of ldhDARSdR and lldp (DA-L)
    Overlap extension PCR of ldhDC and lldp (DC-L)

  • August 20-26

    Ziyi Li, Junhao Xiong, Yizhe Zeng
    Conjugation of E.coli WM3064 and Shewanella oneidensis MR-1: we transferred the targeted plasmid into E.coli WM3064 and conjugated WM3064 and Shewanella oneidensis MR-1 so that the Shewanella oneidensis MR-1 could finally get the targetted plasmid.
    Conjugation pYYDT and pYYDT-lldEFG this week.

    Using device to produce electricity with Shewanella. We proved that Shewanella could produce more electricity with the existence of lactate.

    Yuanda Huang, Haotian Ren, Ziyang Xiao
    PCR of ldhDnARSdR, PCR of ldhDARSdR,
    PCR of ldhDnARSdR, extraction enzyme digestion of ldhDnARSdR,
    Ligation of ldhDnARSdR and psb1c3, transformation
    Hao Qiu
    Constructing the pathway of TH-glad-lldp.
    Insert sequence TH-glad-lldp to vector psb1c3.

  • August 27 – September 2

    Ziyi Li, Junhao Xiong, Yizhe Zeng
    Redesigning about connecting dld and lldEFG: We decided to drop the idea about overlap extension PCR to connect dld and lldEFG and started to use isocaudamer (XbaI and SpeI) to connect these genes.

    Conjugation of pYYDT-gapA and pYYDT-mdh.
    By constructing Rhodopseudomonas palustris-Shewanella oneidensis mutualism system, we found that Shewanella could produce more electricity if Rhodopseudomonas palustris exists.
    Qian Li
    Construction of pSB1C3-RBS-mleS-RBS-lldp.

    Yuanda Huang, Ziyang Xiao
    Overlap extension PCR of ldhDnARSdR-lldp

    Tao Jian
    Adding Flag tag to ldhD-lldP.
    Yan Chen
    Adding Flag tag to ldhDc-lldP

  • September 3-9

    Ziyi Li, Junhao Xiong, Yizhe Zeng, Ziqi Yin
    Conjugation of pYYDT-dld and pYYDT-Ptac-pflB.

    New method to connect dld and lldEFG. However, we failed at the construction of pSB1C3-ptac-lldEFG because of the biobrick prefix on pYYDT.
    We proved that Shewanella could produce more electricty under anaerobic condition.
    Yuanda Huang
    Adding tags to ldhDnARSdR-lldp
    Yan Chen
    Adding his tag to ldhD-lldP and ldhDc-lldP
    Hao Qiu
    Amplifying the gene and transforming in E. coli.
    Ziyang Xiao
    synechocystis culture.

  • September 10-16

    Ziyi Li, Junhao Xiong, Yizhe Zeng, Ziqi Yin
    Conjugation of pYYDT-gapA-mdh

    Haibo Huang
    Adding tags to ldhDnARSdR-lldp
    Yan Chen
    Construction of pmg105-PpckA-RBS-ldhA-TT.

    Yuanda Huang
    Adding tags to ldhDARSdR-lldp
    Yan Chen
    Recombination of ldhD-lldP and ldhDc-lldP to shuttle plasmid PCK306
    Hao Qiu
    Checking the finial consequence by gene sequencing.
    Ziyang Xiao
    synechocystis culture.

  • September 17-23

    Ziyi Li, Junhao Xiong, Yizhe Zeng, Ziqi Yin
    Application of the electrogenesis: we used the device to charge an LED (1.7V), a cell phone (5V) and a small engine (5V). The success proved that we can put our device into practice.
    We proved that carbon cloth has better effect than carbon rods.
    Kaiwen Liu
    Construction of pmg105-PpckA-RBS-mleS-RBS-lldP-TT

    Haibo Huang
    Inserting ldhDnARSdR-lldp biaoda into the plasmid called pck306 through recombinase,
    Inserting ldhDARSdR-lldp biaoda into the plasmid called pck306 through recombinase

    Yan Chen
    Transforming recombinant shuttle plasmid into synechocystis
    Hao Qiu
    Transforming ldhd sequence into synechocystis.
    Ziyang Xiao
    synechocystis culture.

  • September 24-30

    Ziyi Li, Junhao Xiong, Yizhe Zeng, Ziqi Yin
    Construction of pSB1C3-pflB-fdh:we found that there was no tac promotor before.

    We proved that the electricity produced by Rhodopseudomonas palustrls could be ignored in the electrogenesis system.
    At the same time, it was demonstrated that the lactate could be the only carbon source in the culture medium.
    Qian Li
    Construction of pSB1C3- RBS-mleS-RBS-lldP-RBS-ldhA-TT

    Hao Qiu
    Using DNS to detect the condition of producing lactate by synechocystis.
    Yuanda Huang, Yan Chen
    Transforming recombinant shuttle plasmid into synechocystis.
    Ziyang Xiao
    synechocystis culture.

  • October 1-7

    Ziyi Li, Junhao Xiong, Yizhe Zeng, Ziqi Yin
    Construction of pYYDT-pflB-fdh

    Conjugation of pYYDT-pflB-fdh
    We proved that the engineering bacteria with pYYDT-gapA-mdh could produce more electricity than the bacteria with pYYDT.
    Bo Peng
    Construction of RBS-mleS-RBS-lldP-TT pmg105-PpckA-RBS-mleS-RBS-lldP-RBS-ldhA-tt.

    Yuanda Huang
    Overlap extension PCR of ldhDnARSdR and lldp.
    Hao Qiu
    Expansion culture of synechocystis. DNS detection of sugar production of synechocystis, YFP fluorescent protein detection of synechocystis.
    Yan Chen
    Transforming recombinant shuttle plasmid into synechocystis.

  • October 8-14

    Ziyi Li, Junhao Xiong, Yizhe Zeng, Ziqi Yin
    1. Construction of pSB1C3-Ptac-pflB-fdh
    2. Reconjugation of pYYDT-lldEFG
    3. We did two groups of electrogenesis experiments. One of them included the engineering synechocystis and Shewanella, the other included the engineering Rhodopseudomonas palustris and Shewanella. It turned out that the later one worked better than the former one.
    Yuanda Huang
    Add the first tag to ldhDnARSdR-lldp /ldhDARSdR-lldp.
    Hao Qiu
    Detection of YFP fluorescent protein of synechocystis.
    Yan Chen
    Detection of YFP fluorescent protein of synechocystis. Detection of Flag tag.
    Ziyang Xiao
    Detection of YFP fluorescent protein of synechocystis. Detection of 6× His tag and lactate.
    Conjugation of pYYDT-pflB-fdh
    We proved that the engineering bacteria with pYYDT-gapA-mdh could produce more electricity than the bacteria with pYYDT.
    Yuanda Huang
    Overlap extension PCR of ldhDnARSdR and lldp.
    Hao Qiu
    Expansion culture of synechocystis. DNS detection of sugar production of synechocystis, YFP fluorescent protein detection of synechocystis.
    Yan Chen
    Transforming recombinant shuttle plasmid into synechocystis.

College of Life Science & Technology
Huazhong University of Science and Technology
Add: 1037 Luoyu Road, Wuhan, Hubei, China; P.C: 430074
Copyright ©2018 Huazhong University of Science & Technology. Produced By HUST-China