Difference between revisions of "Team:Gifu/Improve"
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| + | <p>As proof of improvement of previous composite part of iGEM, <a href="http://parts.igem.org/Part:BBa_K1491033">BBa_K1491033</a> was provided by iGEM HQ. Typical alignment of T7 promoter is used in this part. However, in PURE system, the enhancer of the stem-loop structure need to be located in the downstream of T7 promoter to make an expression of protein intense. Thus we also design the part (Seq.2) below to show the improvement and this may contribute to the development of synthetic biology and iGEM competition. In Fig.1 we showed the detail of T7 promoter we used this year. Translation enhancer upstream of the SD sequence of mRNA, enhance the biosynthesis of protein. The enhancer may contribute to a direct interaction with ribosome protein S1. Fig.1 shows the detail of the enhancer.</p> | ||
| + | <p>*Caution: BBa_K1332002 contains histidine tag. This time we excluded histidine tag. The function of the coding of this sequence is exactly the same as <a href="http://parts.igem.org/Part:BBa_E1010">BBa_E1010</a>. In Seq.2 it has s stop codon.</p> | ||
| + | <figure> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/f/f7/T--Gifu--improve3.png" width="600px"><br> | ||
| + | <center><legend><b>Seq.2 T7p--SD-RFP-T7t</b></legend></center> | ||
| + | </figure> | ||
| + | <figure> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/0/09/T--Gifu--promoter.png" width="600px"><br> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/6/6e/T--Gifu--BASE.png" width="600px"><br> | ||
| + | <center><legend><b>Fig.1 Explanation of enhancer of T7 promoter</b></legend></center> | ||
| + | </figure> | ||
</div> | </div> | ||
| − | + | http://parts.igem.org/Part:BBa_K1491033 | |
</html> | </html> | ||
Revision as of 23:07, 17 October 2018
As proof of improvement of previous composite part of iGEM, BBa_K1491033 was provided by iGEM HQ. Typical alignment of T7 promoter is used in this part. However, in PURE system, the enhancer of the stem-loop structure need to be located in the downstream of T7 promoter to make an expression of protein intense. Thus we also design the part (Seq.2) below to show the improvement and this may contribute to the development of synthetic biology and iGEM competition. In Fig.1 we showed the detail of T7 promoter we used this year. Translation enhancer upstream of the SD sequence of mRNA, enhance the biosynthesis of protein. The enhancer may contribute to a direct interaction with ribosome protein S1. Fig.1 shows the detail of the enhancer.
*Caution: BBa_K1332002 contains histidine tag. This time we excluded histidine tag. The function of the coding of this sequence is exactly the same as BBa_E1010. In Seq.2 it has s stop codon.
