Difference between revisions of "Template:BOKU-Vienna/Attributions"

 
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<h1 id="attributons">Attributons</h1>
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<h1 id="attributions">Attributions</h1>
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<h2 id="project-timeline">Project timeline</h2>
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<p>The majority of support we received in the planning and implementation of our project came of course from our PIs and instructors. There was an iGEM course held at BOKU, which ran over the course of the summer term. The team members were selected at the beginning of January, right after Christmas break and the course started properly in late February of 2018. In the course each of our instructors gave a lecture on one of the topics we would need for the work on our project. In parallel to the lectures from our instructors the iGEM course was also the setting in which we did most of our brainstorming as a group and decided on a project to pursue. We settled on a project around mid-March and started our lab-work at the beginning of July.</p>
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<h2 id="planning-support">Planning Support</h2>
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<p><strong>Keith Kozminski</strong> has been our amazing advisor guiding our research and keeping us on track for the iGEM Jamboree. From teaching a prepatory iGEM course in the spring to helping us with various aspects of our project, Professor Kozminski&#39;s wise words and advice is an important part of our team. </p>
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<p>Hans Marx, PhD introduced us to golden-gate cloning and showed us how to design parts ourselves. Prof. Brigitte Gasser taught us about the possibilities of genetic logic gates and genetic circuits. Dr. Jürgen Zanghellini showed us how to go about creating a model and helped us with refining ours. Prof. Michael Sauer did a lecture on the many considerations that make up human practices, from bioethics over safety considerations and risk analysis to identifying stakeholders and their concerns. Prof. Diethard Mattanovich introduced us to the basics of project management and made some suggestions on how we could organize ourselves. Prof. Alexandra Graf was our contact for the Wiki and any other informatics questions, but since we have a seasoned programmer and web-designer on our team we didn’t really need any help in that regard. In general all of these lectures were meant to give us the tools to be able to plan and design our project by ourselves, though of course our instructors were always available to us when we had questions and gave us further advice during both the planning and lab-work stages of our project.
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<p><img src="/images/Sponsors/Kozminski.jpg" alt="KeithKozminski">
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One source of support from outside our team of instructors was Prof. Iris Eisenberger who provided support in legal questions and identified potential legal problems our system would encounter in a real world application.</p>
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Keith Kozminski (<em>Photo taken from the Department of Biology at UVA</em>)</p>
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<h2 id="lab-support">Lab support</h2>
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<p><strong>Jason Papin</strong> is our modeling advisor; with his help, we have developed several models that represent various aspects of our project. We are thankful for all the advice and encouragement that Professor Papin has given us throughout our research! </p>
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<p>In our  work with plants we were supported by Dr. Sascha Waidmann a postdoc at BOKU who works in the field of plant biotechnology and with whom we had our first meeting at the end of April. He provided us with Arabidopsis seeds and Agrobacterium and showed us how to grow and transform our plants. His working group, which is led by Prof. Jürgen Kleine-Vehn provided the incubation room for us to grow them.
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<p><img src="/images/Sponsosr/Papin.jpg" alt="JasonPapin">
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During our lab work we were mainly supported by our PIs Prof. Diethard Mattanovich and Hans Marx,PhD who regularly checked on us and made suggestions when we ran into problems. Stefanie Wiesauer ordered our lab materials for us and was very nice about lending us things when we failed to order them in time and also showed us how to use the Nanodrop for measuring DNA concentrations.Prof. Brigitte Gasser showed us how to use the plate reader and Thomas Gaßler, MSc showed us how to use the fluorescence microscope and gave us the protocols we used when working with Pichia pastoris. Dominik Jeschek, Dipl.-Ing. helped us with producing liposomes. For our cooperation with the iGEM Team Stockholm Bernhard Schmelzer, Dipl.-Ing. provided backbones, primers and the template for the alpha factor.  Franziska Doleschal and Birgit Marckhgott were responsible for handling our money. Prof. Michael Sauer also helped us establish communication with the ÖGMBT (Austrian Association of Molecular Life Sciences and Biotechnology), which helped us realise our crowdfunding campaign and allowed us to present our project with a poster at their annual meeting.
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Jason Papin (<em>Photo taken from UVA Today</em>)</p>
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Additionally we would like to thank the whole Sauer working group who occupied the lab next to ours and were always helpful when we had minor questions. They also invited us along to play laser tag with them, which provided a nice break from our lab work. Another group without whom our work in the lab would not have been possible is the cleaning staff responsible for the labs of the Sauer working group who were responsible for deactivating our contaminated waste and cleaning our glassware.</p>
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<h2 id="attributions">Attributions</h2>
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<p><strong>William E. Bentley</strong> is a professor in the Chemical and Biomolecular Engineering department at the University of Maryland and the Distinguished Professor and Chair of the Fischell Department of Bioengineering. Since his research focuses on the expression of biologically active proteins, we have referenced many of his papers within the context of our research. </p>
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<p><img src="/images/Sponsors/Bentley.png" alt="WilliamBentley">
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William E. Bentley (<em>Photo taken from UMD faculty website</em>)</p>
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<p><strong>Drew Endy</strong> is a prominent figure in the synthetic biology community and the professor of bioengineering at Stanford University in California. We are thankful for his help in shipping us a plasmid for our project. </p>
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          <div class="col-4"><a href="https://2018.igem.org/Team:BOKU-Vienna#1"><img src="https://static.igem.org/mediawiki/2018/8/8d/T--BOKU-Vienna--2018_AlexandraGraf.jpg" class="img-responsive"></a></div>
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<p><img src="/images/Sponsors/Endy.jpg" alt="DrewEndy">
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          <div class="col-4"><a href="https://2018.igem.org/Team:BOKU-Vienna#1"><img src="https://static.igem.org/mediawiki/2018/6/65/T--BOKU-Vienna--2018_BernhardSchmelzer.jpg" class="img-responsive"></a></div>
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Drew Endy (<em>Photo taken from indiebio.co</em>)</p>
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          <div class="col-4"><a href="https://2018.igem.org/Team:BOKU-Vienna#1"><img src="https://static.igem.org/mediawiki/2018/d/dd/T--BOKU-Vienna--2018_DiethardMattanovich.jpg" class="img-responsive"></a></div>
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<p><strong>Steven Graff&#39;s</strong> paper, <em>&quot;A Mathematical Model to Study the Role of the Lsr Intergenic Region in Mediation of Autoinducer-2 Quorum Sensing in</em> Escherichia Coli&quot; has been an important reference with regards to our project. After reaching out, Steven Graff has graciously allowed us to use his models and data and given us invaluable advice for our project.</p>
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<p><img src="/images/Sponsors/Graff.jpg" alt="StevenGraff">
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Steven Graff (<em>Photo taken from LinkedIn</em>)</p>
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<p><strong>Dr. Sara Hooshangi</strong> is an associate professor at George Washington University. Her paper, &quot;LsrR Quorum Sensing &#39;Switch&#39; Is Revealed by a Bottom-Up Approach&quot; has been notable reference for modeling our system. </p>
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<p><img src="/images/Sponsors/Hooshangi.png" alt="SaraHooshangi">
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          <div class="col-4"><a href="https://2018.igem.org/Team:BOKU-Vienna#1"><img src="https://static.igem.org/mediawiki/2018/b/b1/T--BOKU-Vienna--2018_ThomasGassler.jpg" class="img-responsive"></a></div>
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Dr. Sara Hooshangi (<em>Photo taken from cps.gwu.edu</em>)</p>
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          <div class="col-4"><a href="https://2018.igem.org/Team:BOKU-Vienna#1"><img src="https://static.igem.org/mediawiki/2018/d/d0/T--BOKU-Vienna--2018_GasserBrigitte.jpg" class="img-responsive"></a></div>
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<p><strong>Shaun Moshasha</strong> is the executive director of <a href="https://www.facebook.com/openbiolabs/">Open Bio Labs</a> in Charlottesville, VA. He is also the Chief Executive Officer at <a href="/Public_Engagement.html">Kombucha Biomaterials LLC</a>. He has been a huge help in guiding our project and has been helping plan our forum this upcoming fall. </p>
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          <div class="col-4"><a href="https://2018.igem.org/Team:BOKU-Vienna#1"><img src="https://static.igem.org/mediawiki/2018/2/26/T--BOKU-Vienna--2018_HansMarx.jpg" class="img-responsive"></a></div>
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<p><img src="/images/Sponsors/Moshasha.jpg" alt="ShaunMoshasha">
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Shaun Moshasha (<em>Photo taken from bio.science.com</em>)</p>
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<p><strong>Charles Richardson</strong> is a postdoctoral student studying Chemical Engineering at the University of Virginia. He is also the Director of Education at Open Bio Labs and has given us valuable advice on the applications, feasibility, and scope of our project. </p>
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<p><img src="/images/Sponsors/Richardson.jpg" alt="CharlesRichardson">
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Charles Richardson (<em>Photo taken from openbiolabs.org</em>)</p>
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          <div class="col-4"><a href="https://2018.igem.org/Team:BOKU-Vienna#1"><img src="https://static.igem.org/mediawiki/2018/c/c3/T--BOKU-Vienna--2018_IrisEisenberger.jpg" class="img-responsive"></a></div>
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<p><strong>Amin Zargar&#39;s</strong> paper, <em>&quot;Enhancing Intercellular Coordination: Rewiring Quorum Sensing Networks for Increased Protein Expression through Autonomous Induction&quot;</em> significantly helped us guide our project. We were fortunate enough to be able to contact him through Skype and ask him for advice on various aspects of our project.</p>
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          <div class="col-4"><a href="https://2018.igem.org/Team:BOKU-Vienna#1"><img src="https://static.igem.org/mediawiki/2018/b/b3/T--BOKU-Vienna--2018_Jurgen-Kleine-Vehn.jpg" class="img-responsive"></a></div>
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<p><img src="/images/Sponsors/Zargar.jpg" alt="AminZargar">
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          <div class="col-4"><a href="https://2018.igem.org/Team:BOKU-Vienna#1"><img src="https://static.igem.org/mediawiki/2018/a/ae/T--BOKU-Vienna--2018_JurgenZanghellini.jpg" class="img-responsive"></a></div>
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Amin Zargar (<em>Photo taken from LinkedIn</em>)</p>
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<p>We would like to thank our sponsors for their support:</p>
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          <div class="col-4"><a href="https://2018.igem.org/Team:BOKU-Vienna#1"><img src="https://static.igem.org/mediawiki/2018/f/fc/T--BOKU-Vienna--2018_DominikJechek.jpg" class="img-responsive"></a></div>
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<h3 id="academic-sponsors">Academic Sponsors</h3>
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          <div class="col-4"><a href="https://2018.igem.org/Team:BOKU-Vienna#1"><img src="https://static.igem.org/mediawiki/2018/0/0a/T--BOKU-Vienna--2018_SaschaWaidmann.jpg" class="img-responsive"></a></div>
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<p><img src="/images/Sponsors/University_Of_Virginia_Logo_transparent.png" alt="UVA"> </p>
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          <div class="col-4"><a href="https://2018.igem.org/Team:BOKU-Vienna#1"><img src="https://static.igem.org/mediawiki/2018/9/99/T--BOKU-Vienna--2018_SauerMichael.jpg" class="img-responsive"></a></div>
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<p>We would like to thank the <strong>University of Virginia</strong> and the <strong>University of Virginia Experiential Learning Fund</strong> for their support. Without them, we would not have the resources to complete our project.</p>
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<p><img src="/images/Sponsors/VPR.jpg" alt="VPR"></p>
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<p><strong>Melur K. Ramasurbramanian</strong>, University of Virginia Vice President of Research has given the Virginia iGEM team a generous grant to allow us to do research over the summer.  </p>
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<p><img src="/images/Sponsors/Genscript_logo_transparent.png" alt="GenScript"> </p>
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Latest revision as of 23:56, 17 October 2018

Attributions

Project timeline

The majority of support we received in the planning and implementation of our project came of course from our PIs and instructors. There was an iGEM course held at BOKU, which ran over the course of the summer term. The team members were selected at the beginning of January, right after Christmas break and the course started properly in late February of 2018. In the course each of our instructors gave a lecture on one of the topics we would need for the work on our project. In parallel to the lectures from our instructors the iGEM course was also the setting in which we did most of our brainstorming as a group and decided on a project to pursue. We settled on a project around mid-March and started our lab-work at the beginning of July.

Planning Support

Hans Marx, PhD introduced us to golden-gate cloning and showed us how to design parts ourselves. Prof. Brigitte Gasser taught us about the possibilities of genetic logic gates and genetic circuits. Dr. Jürgen Zanghellini showed us how to go about creating a model and helped us with refining ours. Prof. Michael Sauer did a lecture on the many considerations that make up human practices, from bioethics over safety considerations and risk analysis to identifying stakeholders and their concerns. Prof. Diethard Mattanovich introduced us to the basics of project management and made some suggestions on how we could organize ourselves. Prof. Alexandra Graf was our contact for the Wiki and any other informatics questions, but since we have a seasoned programmer and web-designer on our team we didn’t really need any help in that regard. In general all of these lectures were meant to give us the tools to be able to plan and design our project by ourselves, though of course our instructors were always available to us when we had questions and gave us further advice during both the planning and lab-work stages of our project. One source of support from outside our team of instructors was Prof. Iris Eisenberger who provided support in legal questions and identified potential legal problems our system would encounter in a real world application.

Lab support

In our work with plants we were supported by Dr. Sascha Waidmann a postdoc at BOKU who works in the field of plant biotechnology and with whom we had our first meeting at the end of April. He provided us with Arabidopsis seeds and Agrobacterium and showed us how to grow and transform our plants. His working group, which is led by Prof. Jürgen Kleine-Vehn provided the incubation room for us to grow them. During our lab work we were mainly supported by our PIs Prof. Diethard Mattanovich and Hans Marx,PhD who regularly checked on us and made suggestions when we ran into problems. Stefanie Wiesauer ordered our lab materials for us and was very nice about lending us things when we failed to order them in time and also showed us how to use the Nanodrop for measuring DNA concentrations.Prof. Brigitte Gasser showed us how to use the plate reader and Thomas Gaßler, MSc showed us how to use the fluorescence microscope and gave us the protocols we used when working with Pichia pastoris. Dominik Jeschek, Dipl.-Ing. helped us with producing liposomes. For our cooperation with the iGEM Team Stockholm Bernhard Schmelzer, Dipl.-Ing. provided backbones, primers and the template for the alpha factor. Franziska Doleschal and Birgit Marckhgott were responsible for handling our money. Prof. Michael Sauer also helped us establish communication with the ÖGMBT (Austrian Association of Molecular Life Sciences and Biotechnology), which helped us realise our crowdfunding campaign and allowed us to present our project with a poster at their annual meeting. Additionally we would like to thank the whole Sauer working group who occupied the lab next to ours and were always helpful when we had minor questions. They also invited us along to play laser tag with them, which provided a nice break from our lab work. Another group without whom our work in the lab would not have been possible is the cleaning staff responsible for the labs of the Sauer working group who were responsible for deactivating our contaminated waste and cleaning our glassware.