Difference between revisions of "Team:Newcastle/Part Collection"

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                     Part Collection <br>
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                     Parts Collection <br>
 
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                            <h3 class="subhead">EXPERIMENTS</h3>
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                 <h1 class="display-2">PARTS</h1>
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                 <h1 class="display-2">New Interlab Parts Collection</h1>
 
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<p style="font-size:21px;">We developed a collection of parts that enable an alternative measurement approach to both the InterLab study and assessing promoter ‘strength’ for other projects. Our parts collection includes an RFP device inserted into the pSB1C3 backbone that acts as an internal standard (IS) for the device being assessed. Four of the Interlab test devices have been returned with this modification. Second, we designed and deposited a new mNeonGreen fluorescent reporter part (<a href="http://parts.igem.org/Part:BBa_K2797003" class='black'>BBa_K2797003</a>) into the registry. This part demonstrated an improvement in the reproducibility over the existing GFPmut3b reporter currently in use in each test device. Five of the Interlab devices were returned with this modification.</p><br></br>
 
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       <td>BBa_K2797002</td>
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       <td><a href="http://parts.igem.org/Part:BBa_K2797003" class='black'>BBa_K2797003</a></td>
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       <td>Composite</td>
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       <td>Basic</td>
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       <td>Composite part for streptomycin resistance</td>
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       <td>mNeonGreen fluorescent protein gene codon optimised for expression in <i>E. coli</i></td>
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       <td>Frank Eardley</td>
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       <td>Kyle Stanforth</td>
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       <td>908</td>
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       <td>711</td>
 
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      <td><a href="http://parts.igem.org/Part:BBa_K2797004" class='black'>BBa_K2797004</a></td>
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      <td>Composite</td>
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      <td>  Test device 1 (originally BBa_J364000) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter </td>
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      <td>Kyle Stanforth</td>
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      <td>909</td>
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      <td><a href="http://parts.igem.org/Part:BBa_K2797005" class='black'>BBa_K2797005</a></td>
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      <td>Composite</td>
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      <td>  Test device 2 (originally BBa_J364001) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter </td>
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      <td>Kyle Stanforth</td>
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      <td>909</td>
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      <td><a href="http://parts.igem.org/Part:BBa_K2797006" class='black'>BBa_K2797006</a></td>
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      <td>Composite</td>
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      <td>  Test device 3 (originally BBa_J364002) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter </td>
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      <td>Kyle Stanforth</td>
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      <td>909</td>
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      <td><a href="http://parts.igem.org/Part:BBa_K2797007" class='black'>BBa_K2797007</a></td>
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      <td>Composite</td>
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      <td>  Test device 4 (originally BBa_J364007) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter </td>
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      <td>Kyle Stanforth</td>
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      <td>909</td>
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      <td><a href="http://parts.igem.org/Part:BBa_K2797008" class='black'>BBa_K2797008</a></td>
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      <td>Composite</td>
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      <td>  Positive control (originally BBa_I20270) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter </td>
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      <td>Kyle Stanforth</td>
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      <td>910</td>
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      <td><a href="http://parts.igem.org/Part:BBa_K2797013" class='black'>BBa_K2797013</a></td>
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      <td>Plasmid vector</td>
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      <td> High copy BioBrick assembly plasmid pSB1C3 with RFP internal standard</td>
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      <td>Kyle Stanforth</td>
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      <td>3073</td>
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      <groupparts>iGEM18 Newcastle</groupparts>
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                <h1 class="display-2">References & Attributions</h1>
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<p class="about-para"><font size="2"><strong>Attributions: Kyle Stanforth
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Latest revision as of 23:57, 17 October 2018

Alternative Roots/Protocols

Alternative Roots

Parts Collection

Overview

New Interlab Parts Collection

We developed a collection of parts that enable an alternative measurement approach to both the InterLab study and assessing promoter ‘strength’ for other projects. Our parts collection includes an RFP device inserted into the pSB1C3 backbone that acts as an internal standard (IS) for the device being assessed. Four of the Interlab test devices have been returned with this modification. Second, we designed and deposited a new mNeonGreen fluorescent reporter part (BBa_K2797003) into the registry. This part demonstrated an improvement in the reproducibility over the existing GFPmut3b reporter currently in use in each test device. Five of the Interlab devices were returned with this modification.



Name Type Description Designer Length(bp)
BBa_K2797003 Basic mNeonGreen fluorescent protein gene codon optimised for expression in E. coli Kyle Stanforth 711
BBa_K2797004 Composite Test device 1 (originally BBa_J364000) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter Kyle Stanforth 909
BBa_K2797005 Composite Test device 2 (originally BBa_J364001) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter Kyle Stanforth 909
BBa_K2797006 Composite Test device 3 (originally BBa_J364002) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter Kyle Stanforth 909
BBa_K2797007 Composite Test device 4 (originally BBa_J364007) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter Kyle Stanforth 909
BBa_K2797008 Composite Positive control (originally BBa_I20270) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter Kyle Stanforth 910
BBa_K2797013 Plasmid vector High copy BioBrick assembly plasmid pSB1C3 with RFP internal standard Kyle Stanforth 3073




References & Attributions

Attributions: Kyle Stanforth