Difference between revisions of "Team:Newcastle/Part Collection"

m
 
(21 intermediate revisions by 5 users not shown)
Line 10: Line 10:
 
     <!-- home
 
     <!-- home
 
     ================================================== -->
 
     ================================================== -->
−
     <section id="home" class="s-home target-section" data-parallax="scroll" data-image-src="https://static.igem.org/mediawiki/2018/2/2d/T--Newcastle--ResultsHeader.jpeg">
+
     <section id="home" class="s-home target-section" data-parallax="scroll" data-image-src="https://static.igem.org/mediawiki/2018/f/f9/T--Newcastle--RFP.png">
  
 
         <div class="overlay"></div>
 
         <div class="overlay"></div>
Line 22: Line 22:
  
 
                 <h1>
 
                 <h1>
−
                     Part Collection <br>
+
                     Parts Collection <br>
 
                     <br>
 
                     <br>
 
                    
 
                    
Line 35: Line 35:
 
             </div>
 
             </div>
  
−
            <div class="home-content__scroll">
 
−
                <a href="#protocol" class="scroll-link smoothscroll">
 
−
                    <span>Scroll Down</span>
 
−
                </a>
 
−
            </div>
 
−
 
−
            <div class="home-content__line"></div>
 
  
 
         </div> <!-- end home-content -->
 
         </div> <!-- end home-content -->
Line 54: Line 47:
 
         <div class="row section-header has-bottom-sep" data-aos="fade-up">
 
         <div class="row section-header has-bottom-sep" data-aos="fade-up">
 
                 <div class="col-full">
 
                 <div class="col-full">
−
                            <h3 class="subhead">EXPERIMENTS</h3>
+
             
−
                 <h1 class="display-2">Designed Parts</h1>
+
                 <h1 class="display-2">New Interlab Parts Collection</h1>
 
             </div>
 
             </div>
 +
<p style="font-size:21px;">We developed a collection of parts that enable an alternative measurement approach to both the InterLab study and assessing promoter ‘strength’ for other projects. Our parts collection includes an RFP device inserted into the pSB1C3 backbone that acts as an internal standard (IS) for the device being assessed. Four of the Interlab test devices have been returned with this modification. Second, we designed and deposited a new mNeonGreen fluorescent reporter part (<a href="http://parts.igem.org/Part:BBa_K2797003" class='black'>BBa_K2797003</a>) into the registry. This part demonstrated an improvement in the reproducibility over the existing GFPmut3b reporter currently in use in each test device. Five of the Interlab devices were returned with this modification.</p><br></br>
 
         </div> <!-- end section-header -->
 
         </div> <!-- end section-header -->
 
<table style=''width:0%>
 
<table style=''width:0%>
Line 67: Line 61:
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
−
       <td><a href="http://parts.igem.org/Part:BBa_K2797002">BBa_K2797002</a></td>
+
       <td><a href="http://parts.igem.org/Part:BBa_K2797003" class='black'>BBa_K2797003</a></td>
−
      <td>Composite</td>
+
−
      <td>Composite part for streptomycin resistance</td>
+
−
      <td>Frank Eardley</td>
+
−
      <td>908</td>
+
−
  </tr>
+
−
  <tr>
+
−
      <td><a href="http://parts.igem.org/Part:BBa_K2797003">BBa_K2797003</a></td>
+
 
       <td>Basic</td>
 
       <td>Basic</td>
−
       <td>mNeonGreen fluorescent protein gene codon optimised for expression in E. coli</td>
+
       <td>mNeonGreen fluorescent protein gene codon optimised for expression in <i>E. coli</i></td>
 
       <td>Kyle Stanforth</td>
 
       <td>Kyle Stanforth</td>
 
       <td>711</td>
 
       <td>711</td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
−
       <td><a href="http://parts.igem.org/Part:BBa_K2797004">BBa_K2797004</a></td>
+
       <td><a href="http://parts.igem.org/Part:BBa_K2797004" class='black'>BBa_K2797004</a></td>
 
       <td>Composite</td>
 
       <td>Composite</td>
 
       <td>  Test device 1 (originally BBa_J364000) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter </td>
 
       <td>  Test device 1 (originally BBa_J364000) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter </td>
Line 88: Line 75:
 
   </tr>  
 
   </tr>  
 
   <tr>
 
   <tr>
−
       <td><a href="http://parts.igem.org/Part:BBa_K2797005">BBa_K2797005</a></td>
+
       <td><a href="http://parts.igem.org/Part:BBa_K2797005" class='black'>BBa_K2797005</a></td>
 
       <td>Composite</td>
 
       <td>Composite</td>
 
       <td>  Test device 2 (originally BBa_J364001) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter </td>
 
       <td>  Test device 2 (originally BBa_J364001) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter </td>
Line 95: Line 82:
 
   </tr>  
 
   </tr>  
 
   <tr>
 
   <tr>
−
       <td><a href="http://parts.igem.org/Part:BBa_K2797006">BBa_K2797006</a></td>
+
       <td><a href="http://parts.igem.org/Part:BBa_K2797006" class='black'>BBa_K2797006</a></td>
 
       <td>Composite</td>
 
       <td>Composite</td>
 
       <td>  Test device 3 (originally BBa_J364002) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter </td>
 
       <td>  Test device 3 (originally BBa_J364002) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter </td>
Line 102: Line 89:
 
   </tr>  
 
   </tr>  
 
   <tr>
 
   <tr>
−
       <td><a href="http://parts.igem.org/Part:BBa_K2797007">BBa_K2797007</a></td>
+
       <td><a href="http://parts.igem.org/Part:BBa_K2797007" class='black'>BBa_K2797007</a></td>
 
       <td>Composite</td>
 
       <td>Composite</td>
 
       <td>  Test device 4 (originally BBa_J364007) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter </td>
 
       <td>  Test device 4 (originally BBa_J364007) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter </td>
Line 109: Line 96:
 
   </tr>  
 
   </tr>  
 
   <tr>
 
   <tr>
−
       <td><a href="http://parts.igem.org/Part:BBa_K2797008">BBa_K2797008</a></td>
+
       <td><a href="http://parts.igem.org/Part:BBa_K2797008" class='black'>BBa_K2797008</a></td>
 
       <td>Composite</td>
 
       <td>Composite</td>
 
       <td>  Positive control (originally BBa_I20270) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter </td>
 
       <td>  Positive control (originally BBa_I20270) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter </td>
Line 116: Line 103:
 
   </tr>  
 
   </tr>  
 
   <tr>
 
   <tr>
−
       <td><a href="http://parts.igem.org/Part:BBa_K2797013">BBa_K2797013</a></td>
+
       <td><a href="http://parts.igem.org/Part:BBa_K2797013" class='black'>BBa_K2797013</a></td>
 
       <td>Plasmid vector</td>
 
       <td>Plasmid vector</td>
 
       <td> High copy BioBrick assembly plasmid pSB1C3 with RFP internal standard</td>
 
       <td> High copy BioBrick assembly plasmid pSB1C3 with RFP internal standard</td>
Line 128: Line 115:
  
 
<table id="protocols">
 
<table id="protocols">
−
      <groupparts>iGEM18 Newcastle</groupparts>
+
 
 
     </table>
 
     </table>
  
Line 175: Line 162:
  
 
     </div> <!-- end photoSwipe background -->
 
     </div> <!-- end photoSwipe background -->
 +
<section id='team' class="s-services">
 +
 +
 +
        <div class="row section-header has-bottom-sep" data-aos="fade-up">
 +
                <div class="col-full">
 +
                            <br>
 +
<br>
 +
<br>
 +
<br>
 +
<h3 class="subhead"></h3>
 +
                <h1 class="display-2">References & Attributions</h1>
 +
            </div>
 +
 +
        </div>
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
<button class="collapsible">Click for References & Attributions</button>
 +
<div class="content">
 +
              <div class="row about-desc" data-aos="fade-up">
 +
                <div class="col-full">
 +
 +
<p class="about-para"><font size="2"><strong>Attributions: Kyle Stanforth
 +
</strong><font></p>
 +
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</section>
  
 
    
 
    
Line 185: Line 211:
 
     <script type="text/javascript" src="https://2018.igem.org/Template:Newcastle/JSmain?
 
     <script type="text/javascript" src="https://2018.igem.org/Template:Newcastle/JSmain?
 
     action=raw&ctype=text/javascript"></script>   
 
     action=raw&ctype=text/javascript"></script>   
 +
    <script>
 +
var coll = document.getElementsByClassName("collapsible");
 +
var i;
  
 +
for (i = 0; i < coll.length; i++) {
 +
  coll[i].addEventListener("click", function() {
 +
    this.classList.toggle("active");
 +
    var content = this.nextElementSibling;
 +
    if (content.style.maxHeight){
 +
      content.style.maxHeight = null;
 +
    } else {
 +
      content.style.maxHeight = content.scrollHeight + "px";
 +
    }
 +
  });
 +
}
 +
</script>
 
</body>
 
</body>
  
 
</html>
 
</html>
 
{{Newcastle/footer}}
 
{{Newcastle/footer}}

Latest revision as of 23:57, 17 October 2018

Alternative Roots/Protocols

Alternative Roots

Parts Collection

Overview

New Interlab Parts Collection

We developed a collection of parts that enable an alternative measurement approach to both the InterLab study and assessing promoter ‘strength’ for other projects. Our parts collection includes an RFP device inserted into the pSB1C3 backbone that acts as an internal standard (IS) for the device being assessed. Four of the Interlab test devices have been returned with this modification. Second, we designed and deposited a new mNeonGreen fluorescent reporter part (BBa_K2797003) into the registry. This part demonstrated an improvement in the reproducibility over the existing GFPmut3b reporter currently in use in each test device. Five of the Interlab devices were returned with this modification.



Name Type Description Designer Length(bp)
BBa_K2797003 Basic mNeonGreen fluorescent protein gene codon optimised for expression in E. coli Kyle Stanforth 711
BBa_K2797004 Composite Test device 1 (originally BBa_J364000) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter Kyle Stanforth 909
BBa_K2797005 Composite Test device 2 (originally BBa_J364001) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter Kyle Stanforth 909
BBa_K2797006 Composite Test device 3 (originally BBa_J364002) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter Kyle Stanforth 909
BBa_K2797007 Composite Test device 4 (originally BBa_J364007) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter Kyle Stanforth 909
BBa_K2797008 Composite Positive control (originally BBa_I20270) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter Kyle Stanforth 910
BBa_K2797013 Plasmid vector High copy BioBrick assembly plasmid pSB1C3 with RFP internal standard Kyle Stanforth 3073




References & Attributions

Attributions: Kyle Stanforth