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<h3>Safe Project Design</h3> | <h3>Safe Project Design</h3> | ||
− | <p> | + | <p>Since our team had never before competed in iGEM, we decided to stick to very safe species: horseshoe crabs, E. coli K12 strains, and the industrial yeast Pichia pastoris for our project. </p> |
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− | <li> | + | <li>For all cloning we chose non-pathogenic chassis organisms</li> |
− | <li> | + | <li>We chose to express single proteins rather than pathways and focus on diagnostics rather than therapeutics</li> |
− | <li> | + | <li>For our proof-of-concept experiment we used E.coli K12 rather than native E. coli as a source of endotoxins</li> |
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<h3>Safe Lab Work</h3> | <h3>Safe Lab Work</h3> | ||
− | <p> | + | <p>We wore personal protective gear appropriate to the organisms and chemicals we worked with, and were careful to clean up after experiments with 70% ethanol on the benchtops. when we had to transport our Pichia pastoris to Columbia University for electroporation, we carried it in capped tubes sealed in a styrofoam box. </p> |
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Latest revision as of 00:03, 18 October 2018
>
Safe Project Design
Since our team had never before competed in iGEM, we decided to stick to very safe species: horseshoe crabs, E. coli K12 strains, and the industrial yeast Pichia pastoris for our project.
- For all cloning we chose non-pathogenic chassis organisms
- We chose to express single proteins rather than pathways and focus on diagnostics rather than therapeutics
- For our proof-of-concept experiment we used E.coli K12 rather than native E. coli as a source of endotoxins
Safe Lab Work
We wore personal protective gear appropriate to the organisms and chemicals we worked with, and were careful to clean up after experiments with 70% ethanol on the benchtops. when we had to transport our Pichia pastoris to Columbia University for electroporation, we carried it in capped tubes sealed in a styrofoam box.
</html>