Difference between revisions of "Team:Bio Without Borders/Safety"

(Prototype team page)
 
 
(4 intermediate revisions by one other user not shown)
Line 5: Line 5:
 
<div class="column full_size">
 
<div class="column full_size">
  
−
<h1> Safety </h1>
 
−
<p>Please visit the <a href="https://2018.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
 
−
 
−
<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
 
  
 
</div>
 
</div>
Line 14: Line 10:
  
 
<div class="column two_thirds_size">
 
<div class="column two_thirds_size">
 +
>
 +
 
<h3>Safe Project Design</h3>
 
<h3>Safe Project Design</h3>
  
−
<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
+
<p>Since our team had never before competed in iGEM, we decided to stick to very safe species: horseshoe crabs, E. coli K12 strains, and the industrial yeast Pichia pastoris for our project. </p>
  
 
<ul>
 
<ul>
−
<li>Choosing a non-pathogenic chassis</li>
+
<li>For all cloning we chose non-pathogenic chassis organisms</li>
−
<li>Choosing parts that will not harm humans / animals / plants</li>
+
<li>We chose to express single proteins rather than pathways and focus on diagnostics rather than therapeutics</li>
−
<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
+
<li>For our proof-of-concept experiment we used E.coli K12 rather than native E. coli as a source of endotoxins</li>
−
<li>Including an "induced lethality" or "kill-switch" device</li>
+
 
 
</ul>
 
</ul>
  
Line 30: Line 28:
 
<h3>Safe Lab Work</h3>
 
<h3>Safe Lab Work</h3>
  
−
<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
+
<p>We wore personal protective gear appropriate to the organisms and chemicals we worked with, and were careful to clean up after experiments with 70% ethanol on the benchtops. when we had to transport our Pichia pastoris to Columbia University for electroporation, we carried it in capped tubes sealed in a styrofoam box. </p>
  
  
−
<h3>Safe Shipment</h3>
 
−
 
−
<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
 
</div>
 
</div>
 +
 +
 +
</html>
  
  
 
</html>
 
</html>

Latest revision as of 00:03, 18 October 2018

>

Safe Project Design

Since our team had never before competed in iGEM, we decided to stick to very safe species: horseshoe crabs, E. coli K12 strains, and the industrial yeast Pichia pastoris for our project.

  • For all cloning we chose non-pathogenic chassis organisms
  • We chose to express single proteins rather than pathways and focus on diagnostics rather than therapeutics
  • For our proof-of-concept experiment we used E.coli K12 rather than native E. coli as a source of endotoxins

Safe Lab Work

We wore personal protective gear appropriate to the organisms and chemicals we worked with, and were careful to clean up after experiments with 70% ethanol on the benchtops. when we had to transport our Pichia pastoris to Columbia University for electroporation, we carried it in capped tubes sealed in a styrofoam box.


</html>