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− | <div class=" | + | <section id="two" class="wrapper alt style2"> |
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− | <p> | + | <div class="image"><img src="https://static.igem.org/mediawiki/2018/5/55/T--Bio_Without_Borders--HorseshoeCrabs.png" alt="" /></div><div class="content"> |
− | + | <h2>Creating Factor C<br /></h2> | |
− | </p> | + | <p>We used an NEBuilder kit to combine two sequence that IDT sent us.</p> |
+ | </div> | ||
+ | </section> | ||
+ | <section class="spotlight"> | ||
+ | <div class="image"><img src="https://static.igem.org/mediawiki/parts/6/6b/T--Bio_Without_Borders--Outreach_1.png" alt="" /></div><div class="content"> | ||
+ | <h2>Creating a linker protein of the cellulose-binding domain (CBD) and GFP | ||
+ | </h2> | ||
+ | <p> This functions as the reporter protein which would demonstrate Factor C's ability to cleave.</p> | ||
+ | </div> | ||
+ | </section> | ||
+ | <section class="spotlight"> | ||
+ | <div class="image"><img src="https://static.igem.org/mediawiki/2018/7/72/T--Bio_Without_Borders--Team.png" alt="" /></div><div class="content"> | ||
+ | <h2>Expressing Factor C through Pichia pastoris</h2> | ||
+ | <p> After making our parts, we wanted to express the parts in an easy model organism like pichia. </p> | ||
+ | </div> | ||
+ | </section> | ||
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Revision as of 00:08, 18 October 2018
Creating Factor C
We used an NEBuilder kit to combine two sequence that IDT sent us.
Creating a linker protein of the cellulose-binding domain (CBD) and GFP
This functions as the reporter protein which would demonstrate Factor C's ability to cleave.
Expressing Factor C through Pichia pastoris
After making our parts, we wanted to express the parts in an easy model organism like pichia.