Difference between revisions of "Team:IISER-Bhopal-India"
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| − | <img src="https://static.igem.org/mediawiki/2018/ | + | |
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| + | <img src="https://static.igem.org/mediawiki/2018/8/8a/T--IISER-Bhopal-India--slidermethfinal1.png" alt="" /> | ||
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| − | < | + | <li><a href="https://2018.igem.org/Team:IISER-Bhopal-India/Idea"> |
| − | + | <img src="https://static.igem.org/mediawiki/2018/a/a8/T--IISER-Bhopal-India--methslider2final.png" alt="" /> | |
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| − | </ | + | <li><a href="https://2018.igem.org/Team:IISER-Bhopal-India/approach"> |
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| + | <li><a href="https://2018.igem.org/Team:IISER-Bhopal-India/Results"> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/6/66/T--IISER-Bhopal-India--slidermethfinal4.png" alt="" /> | ||
| + | </a> | ||
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| − | <li> | + | <li><a href="https://2018.igem.org/Team:IISER-Bhopal-India/Prospects"> |
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| − | <p align="justify" style="font-family:'Roboto', sans-serif; font-size:16px; font-weight:300; line-height:1.6em; color:#656565;">Methane is a | + | <p align="justify" style="font-family:'Roboto', sans-serif; font-size:16px; font-weight:300; line-height:1.6em; color:#656565;">Methane is a growing concern in today's scenario and green methods are desired for its real-time monitoring. |
Thus, we have developed the prototype of a robust field-applicable methane biosensor, | Thus, we have developed the prototype of a robust field-applicable methane biosensor, | ||
| − | MethNote. We found an enzyme-complex methane monooxygenase(MMO) from Methylococcus capsulatus, | + | <b>MethNote</b>. We found an enzyme-complex methane monooxygenase(MMO) from <i>Methylococcus capsulatus</i>, |
a methanotrophic bacterium, that converts methane to methanol. We expressed soluble-MMO in the methylotrophic yeast, | a methanotrophic bacterium, that converts methane to methanol. We expressed soluble-MMO in the methylotrophic yeast, | ||
| − | Pichia pastoris, which harbors a plasmid expressing the reporter gene under a methanol inducible promoter AOX. | + | <i>Pichia pastoris</i>, which harbors a plasmid expressing the reporter gene under a methanol inducible promoter AOX. |
Thus, linking methane uptake to a reporter gene expression generates the proposed methane biosensor. The inclusion of | Thus, linking methane uptake to a reporter gene expression generates the proposed methane biosensor. The inclusion of | ||
sMMO pathway was also checked by metabolic modeling. The constructed part will be a useful contribution to the iGEM repository. | sMMO pathway was also checked by metabolic modeling. The constructed part will be a useful contribution to the iGEM repository. | ||
A commercial design of MethNote will find widespread applications in environmental monitoring of methane. In future studies, | A commercial design of MethNote will find widespread applications in environmental monitoring of methane. In future studies, | ||
| − | we also anticipate an additional application of Mut- strain of P. pastoris expressing sMMO in biofuel production through | + | we also anticipate an additional application of Mut- strain of <i>P. pastoris</i> expressing sMMO in biofuel production through |
methanol sequestration.</p> | methanol sequestration.</p> | ||
</div> | </div> | ||
Latest revision as of 00:37, 18 October 2018
Overview
Methane is a growing concern in today's scenario and green methods are desired for its real-time monitoring. Thus, we have developed the prototype of a robust field-applicable methane biosensor, MethNote. We found an enzyme-complex methane monooxygenase(MMO) from Methylococcus capsulatus, a methanotrophic bacterium, that converts methane to methanol. We expressed soluble-MMO in the methylotrophic yeast, Pichia pastoris, which harbors a plasmid expressing the reporter gene under a methanol inducible promoter AOX. Thus, linking methane uptake to a reporter gene expression generates the proposed methane biosensor. The inclusion of sMMO pathway was also checked by metabolic modeling. The constructed part will be a useful contribution to the iGEM repository. A commercial design of MethNote will find widespread applications in environmental monitoring of methane. In future studies, we also anticipate an additional application of Mut- strain of P. pastoris expressing sMMO in biofuel production through methanol sequestration.