Difference between revisions of "Team:UPF CRG Barcelona/Intermediate"

(Created page with "<html> <link rel="stylesheet" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/styles?action=raw&ctype=text/css" type='text/css'> <body> <div class="sticky-top"> <n...")
 
 
(7 intermediate revisions by 2 users not shown)
Line 2: Line 2:
  
 
  <link rel="stylesheet" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/styles?action=raw&ctype=text/css" type='text/css'>
 
  <link rel="stylesheet" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/styles?action=raw&ctype=text/css" type='text/css'>
−
 
  
 
<body>
 
<body>
Line 10: Line 9:
  
 
         <div class="navbar-brand">
 
         <div class="navbar-brand">
−
           <a class="navbar-logo" href="#">
+
           <a class="navbar-logo" href="https://2018.igem.org/Team:UPF_CRG_Barcelona">
−
             <img src="assets/T--UPF_CRG_Barcelona--logosensebarcelona.svg" alt="iGEM Barcelona team 2018">
+
             <img src="https://static.igem.org/mediawiki/2018/3/37/T--UPF_CRG_Barcelona--logosensebarcelona.svg" alt="iGEM Barcelona team 2018">
 
           </a>
 
           </a>
 
           <div class="navbar-burger" data-target="navbar-menu">
 
           <div class="navbar-burger" data-target="navbar-menu">
Line 25: Line 24:
 
           <div class="navbar-grid">
 
           <div class="navbar-grid">
 
             <!--class is-active for showing active menu-->
 
             <!--class is-active for showing active menu-->
−
             <a class="navbar-item" href="Description.html">Project</a>
+
             <a class="navbar-item" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Description">Project</a>
−
             <a class="navbar-item" href="DryLab.html">DryLab</a>
+
             <a class="navbar-item" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/DryLab.html">DryLab</a>
−
             <a class="navbar-item is-active" href="Parts.html">Parts</a>
+
             <a class="navbar-item is-active" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Parts">Parts</a>
−
             <a class="navbar-item" href="Deliverables.html">Deliverables</a>
+
             <a class="navbar-item" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Deliverables">Deliverables</a>
−
             <a class="navbar-item" href="WetLab.html">WetLab</a>
+
             <a class="navbar-item" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/WetLab">WetLab</a>
−
             <a class="navbar-item" href="Intro_HP.html">Human Practices</a>
+
             <a class="navbar-item" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Intro_HP">Human Practices</a>
−
             <a class="navbar-item" href="Aboutteam.html">Team</a>
+
             <a class="navbar-item" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Aboutteam">Team</a>
 
           </div>
 
           </div>
 
         </div>
 
         </div>
Line 42: Line 41:
 
       <div class="swiper-container" id="sw-container">
 
       <div class="swiper-container" id="sw-container">
 
         <div class="swiper-wrapper">
 
         <div class="swiper-wrapper">
−
           <a class="swiper-slide swiper-slide-menu" href="Basic.html">
+
           <a class="swiper-slide swiper-slide-menu" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Basic">
−
             <embed style="width: 50px" src="assets/T--UPF_CRG_Barcelona--basicpart.svg" alt="">
+
             <embed src="https://static.igem.org/mediawiki/2018/2/25/T--UPF_CRG_Barcelona--basicpart.svg" alt="">
 
             <p>Basic Parts</p>
 
             <p>Basic Parts</p>
 
           </a>
 
           </a>
−
           <a class="swiper-slide swiper-slide-menu" href="Intermediate.html">
+
           <a class="swiper-slide swiper-slide-menu" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Intermediate">
−
             <img style="height: 50px" src="assets/T--UPF_CRG_Barcelona--compositepart.svg" alt="">
+
             <embed src="https://2018.igem.org//wiki/images/e/e8/T--UPF_CRG_Barcelona--partsdeliverables_navbar.svg" alt="">
 
             <p>Construction<br>Intermediates</p>
 
             <p>Construction<br>Intermediates</p>
 
           </a>
 
           </a>
−
           <a class="swiper-slide swiper-slide-menu" href="Composite.html">
+
           <a class="swiper-slide swiper-slide-menu" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Composite">
−
             <embed style="width: 50px" src="assets/T--UPF_CRG_Barcelona--compositepart.svg" alt="">
+
             <embed src="https://static.igem.org/mediawiki/2018/e/ed/T--UPF_CRG_Barcelona--compositepart.svg" alt="">
 
             <p>Composite Parts</p>
 
             <p>Composite Parts</p>
 
           </a>
 
           </a>
−
           <a class="swiper-slide swiper-slide-menu" href="Improve.html">
+
           <a class="swiper-slide swiper-slide-menu" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Improve">
−
             <embed style="width: 50px" src="assets--UPF_CRG_Barcelona--improvedpart_1.svg" alt="">
+
             <embed src="https://static.igem.org/mediawiki/2018/9/96/T--UPF_CRG_Barcelona--improvedpart.svg" alt="">
 
             <p>Improved Parts</p>
 
             <p>Improved Parts</p>
 
           </a>
 
           </a>
−
           <a class="swiper-slide swiper-slide-menu" href="Registry.html">
+
           <a class="swiper-slide swiper-slide-menu" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Registry">
−
             <embed style="width: 50px" src="assets/T--UPF_CRG_Barcelona--registryparts.svg" alt="">
+
             <embed src="https://static.igem.org/mediawiki/2018/f/f5/T--UPF_CRG_Barcelona--registryparts.svg" alt="">
 
             <p>Parts Registry</p>
 
             <p>Parts Registry</p>
 
           </a>
 
           </a>
Line 76: Line 75:
 
       <div>
 
       <div>
 
         <p class="page-title">CONSTRUCTION INTERMEDIATES</p>
 
         <p class="page-title">CONSTRUCTION INTERMEDIATES</p>
−
         <p><b>Bba_K2581000: T14_pfadBA2</b></p>
+
         <p><b>Bba_K2581000: pFadBA with weak RBS and double terminator</b></p>
 
         <p>It is the construction intermediate used in the BBa_K2581004 part. It consists of the assembly of a double
 
         <p>It is the construction intermediate used in the BBa_K2581004 part. It consists of the assembly of a double
 
           terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter
 
           terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter
 
           which expresses the downstream gene in the presence of fatty acids (BBa_K817002), and a weak RBS (BBa_B0032).
 
           which expresses the downstream gene in the presence of fatty acids (BBa_K817002), and a weak RBS (BBa_B0032).
 
         </p>
 
         </p>
−
         <center><img src="/assets/T--UPF_CRG_Barcelona--intermediateconstruction_1.svg" style="width: 35%;"></center>
+
         <center><img src="https://static.igem.org/mediawiki/2018/7/75/T--UPF_CRG_Barcelona--intermediateconstruction_1.svg" style="width: 35%;"></center>
−
         <p><b>BBa_K2581001: T14_pfadBA3</b></p>
+
         <p><b>BBa_K2581001: pFadBA with weak RBS and double terminator</b></p>
 
         <p>This is the construction intermediate used in the BBa_K2581005 part. It consists of the assembly of a double
 
         <p>This is the construction intermediate used in the BBa_K2581005 part. It consists of the assembly of a double
 
           terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter
 
           terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter
 
           which expresses the downstream gene in the presence of fatty acids (BBa_K817002), and a weak RBS (BBa_B0033).</p>
 
           which expresses the downstream gene in the presence of fatty acids (BBa_K817002), and a weak RBS (BBa_B0033).</p>
−
         <center><img src="/assets/T--UPF_CRG_Barcelona--intermediateconstruction.svg" style="width: 35%;"></center>
+
         <center><img src="https://static.igem.org/mediawiki/2018/b/bf/T--UPF_CRG_Barcelona--intermediateconstruction_2.svg" style="width: 35%;"></center>
−
         <p><b>BBa_K2581002: T14_pfadBA4</b></p>
+
         <p><b>BBa_K2581002: pFadBA with strong RBS and double terminator</b></p>
 
         <p>This is the construction intermediate used in the BBa_K2581006 part. It consists of the assembly of a double
 
         <p>This is the construction intermediate used in the BBa_K2581006 part. It consists of the assembly of a double
 
           terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter
 
           terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter
Line 93: Line 92:
 
           (BBa_B0034).
 
           (BBa_B0034).
 
         </p>
 
         </p>
−
         <center><img src="/assets/T--UPF_CRG_Barcelona--intermediateconstruction.svg" style="width: 35%;"></center>
+
         <center><img src="https://static.igem.org/mediawiki/2018/b/bd/T--UPF_CRG_Barcelona--intermediateconstruction_3.svg" style="width: 35%;"></center>
−
         <p><b>BBa_K2581003: 32_fadD</b></p>
+
         <p><b>BBa_K2581003: FadD with weak RBS</b></p>
 
         <p>This is the construction intermediate used in the BBa_K2581009 part. It consists in the assembly of the weak
 
         <p>This is the construction intermediate used in the BBa_K2581009 part. It consists in the assembly of the weak
 
           RBS 32 (BBa_B0032) and the FadD gene (BBa_K2581016) obtained of the own <i>E. coli</i> DH5-alpha strain
 
           RBS 32 (BBa_B0032) and the FadD gene (BBa_K2581016) obtained of the own <i>E. coli</i> DH5-alpha strain
 
           genome.
 
           genome.
 
         </p>
 
         </p>
−
         <center><img src="/assets/T--UPF_CRG_Barcelona--intermediateconstruction_4.svg" style="width: 35%;"></center>
+
         <center><img src="https://static.igem.org/mediawiki/2018/f/fc/T--UPF_CRG_Barcelona--intermediateconstruction_4.svg" style="width: 35%;"></center>
−
         <p><b>BBa_K2581013: T14_pAR</b></p>
+
         <p><b>BBa_K2581013: pAR: Improved fatty acyl-CoA responsive promoter</b></p>
 
         <p>The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty
 
         <p>The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty
 
           acid intracellular promoter pFadBA (BBa_k817002). This new promoter has two binding sites for FadR in
 
           acid intracellular promoter pFadBA (BBa_k817002). This new promoter has two binding sites for FadR in
Line 108: Line 107:
 
           promoter based on the previous pFadBA DNA sequence.
 
           promoter based on the previous pFadBA DNA sequence.
 
         </p>
 
         </p>
−
         <center><img src="/assets/T--UPF_CRG_Barcelona--t14par.svg" alt="T14_pAR part" style="width: 35%"></center>
+
         <center><img src="https://static.igem.org/mediawiki/2018/1/15/T--UPF_CRG_Barcelona--t14par.svg" alt="T14_pAR part" style="width: 35%"></center>
−
         <p><b>BBa_K2581017: T14_pFadBA4_CI_T14_prm4_RFP or Intermediate fatty acid biosensor with sharp response
+
         <p><b>BBa_K2581017: Intermediate fatty acid biosensor with sharp response
 
           </b></p>
 
           </b></p>
 
         <p>The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid uptake
 
         <p>The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid uptake
Line 120: Line 119:
 
           (BBa_E1010).
 
           (BBa_E1010).
 
         </p>
 
         </p>
−
         <center><img src="/assets/T--UPF_CRG_Barcelona--highpassfilter34.svg" alt="T14_pAR part" style="width: 55%"></center>
+
         <center><img src="https://static.igem.org/mediawiki/2018/1/14/T--UPF_CRG_Barcelona--highpassfilter34.svg" alt="T14_pAR part" style="width: 55%"></center>
−
         <p><b>BBa_K2581018: T14_pFadBA2_CI_T14_prm2_RFP or Intermediate fatty acid biosensor with sharp response
+
         <p><b>BBa_K2581018: Intermediate fatty acid biosensor with sharp response
 
           </b></p>
 
           </b></p>
 
         <p>The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid uptake
 
         <p>The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid uptake
Line 132: Line 131:
 
           (BBa_B0032), and an engineered mutant of red fluorescent protein from <i>Discosoma striata</i> (BBa_E1010).
 
           (BBa_B0032), and an engineered mutant of red fluorescent protein from <i>Discosoma striata</i> (BBa_E1010).
 
         </p>
 
         </p>
−
         <center><img src="/assets/T--UPF_CRG_Barcelona--highpassfilter32.svg" alt="T14_pAR part" style="width: 55%"></center>
+
         <center><img src="https://static.igem.org/mediawiki/2018/d/de/T--UPF_CRG_Barcelona--highpassfilter32.svg" alt="T14_pAR part" style="width: 55%"></center>
  
−
         <br>
+
         <div class="spacer"></div>
−
        <br>
+
−
        <br>
+
−
        <br>
+
 
       </div>
 
       </div>
  

Latest revision as of 00:41, 18 October 2018

CONSTRUCTION INTERMEDIATES

Bba_K2581000: pFadBA with weak RBS and double terminator

It is the construction intermediate used in the BBa_K2581004 part. It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_K817002), and a weak RBS (BBa_B0032).

BBa_K2581001: pFadBA with weak RBS and double terminator

This is the construction intermediate used in the BBa_K2581005 part. It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_K817002), and a weak RBS (BBa_B0033).

BBa_K2581002: pFadBA with strong RBS and double terminator

This is the construction intermediate used in the BBa_K2581006 part. It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_K817002), and a strong RBS (BBa_B0034).

BBa_K2581003: FadD with weak RBS

This is the construction intermediate used in the BBa_K2581009 part. It consists in the assembly of the weak RBS 32 (BBa_B0032) and the FadD gene (BBa_K2581016) obtained of the own E. coli DH5-alpha strain genome.

BBa_K2581013: pAR: Improved fatty acyl-CoA responsive promoter

The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty acid intracellular promoter pFadBA (BBa_k817002). This new promoter has two binding sites for FadR in comparison with the existing part. Therefore, we have demonstrate an enhanced function of this promoter resulting in a disminution of basility and hence, an improvement of this part. This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), and the improved promoter based on the previous pFadBA DNA sequence.

T14_pAR part

BBa_K2581017: Intermediate fatty acid biosensor with sharp response

The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid uptake consisting in a high pass filter in order to reduce the high basal activity of the promoter in another efficient way. It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_K817002), a strong RBS (BBa_B0034), a CI repressor from E. coli phage lambda (+ LVA) (BBa_C0051), again the double terminator (BBa_B0014), a modified lambda Prm promoter (BBa_I12036), again the strong RBS (BBa_B0034) and an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).

T14_pAR part

BBa_K2581018: Intermediate fatty acid biosensor with sharp response

The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid uptake consisting in a high pass filter in order to reduce the high basal activity of the promoter in another efficient way. It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_K817002), a weak RBS (BBa_B0032), a CI repressor from E. coli phage lambda (+LVA) (BBa_C0051), again the double terminator (BBa_B0014), a modified lambda Prm promoter (BBa_I12036), again the weak RBS (BBa_B0032), and an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).

T14_pAR part

Wiki