Difference between revisions of "Team:UPF CRG Barcelona/Intermediate"
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<img src="https://static.igem.org/mediawiki/2018/3/37/T--UPF_CRG_Barcelona--logosensebarcelona.svg" alt="iGEM Barcelona team 2018"> | <img src="https://static.igem.org/mediawiki/2018/3/37/T--UPF_CRG_Barcelona--logosensebarcelona.svg" alt="iGEM Barcelona team 2018"> | ||
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<div class="swiper-wrapper"> | <div class="swiper-wrapper"> | ||
| − | <a class="swiper-slide swiper-slide-menu" href=" | + | <a class="swiper-slide swiper-slide-menu" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Basic"> |
<embed src="https://static.igem.org/mediawiki/2018/2/25/T--UPF_CRG_Barcelona--basicpart.svg" alt=""> | <embed src="https://static.igem.org/mediawiki/2018/2/25/T--UPF_CRG_Barcelona--basicpart.svg" alt=""> | ||
<p>Basic Parts</p> | <p>Basic Parts</p> | ||
</a> | </a> | ||
| − | <a class="swiper-slide swiper-slide-menu" href=" | + | <a class="swiper-slide swiper-slide-menu" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Intermediate"> |
<embed src="https://2018.igem.org//wiki/images/e/e8/T--UPF_CRG_Barcelona--partsdeliverables_navbar.svg" alt=""> | <embed src="https://2018.igem.org//wiki/images/e/e8/T--UPF_CRG_Barcelona--partsdeliverables_navbar.svg" alt=""> | ||
<p>Construction<br>Intermediates</p> | <p>Construction<br>Intermediates</p> | ||
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<embed src="https://static.igem.org/mediawiki/2018/e/ed/T--UPF_CRG_Barcelona--compositepart.svg" alt=""> | <embed src="https://static.igem.org/mediawiki/2018/e/ed/T--UPF_CRG_Barcelona--compositepart.svg" alt=""> | ||
<p>Composite Parts</p> | <p>Composite Parts</p> | ||
</a> | </a> | ||
| − | <a class="swiper-slide swiper-slide-menu" href=" | + | <a class="swiper-slide swiper-slide-menu" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Improve"> |
<embed src="https://static.igem.org/mediawiki/2018/9/96/T--UPF_CRG_Barcelona--improvedpart.svg" alt=""> | <embed src="https://static.igem.org/mediawiki/2018/9/96/T--UPF_CRG_Barcelona--improvedpart.svg" alt=""> | ||
<p>Improved Parts</p> | <p>Improved Parts</p> | ||
</a> | </a> | ||
| − | <a class="swiper-slide swiper-slide-menu" href=" | + | <a class="swiper-slide swiper-slide-menu" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Registry"> |
<embed src="https://static.igem.org/mediawiki/2018/f/f5/T--UPF_CRG_Barcelona--registryparts.svg" alt=""> | <embed src="https://static.igem.org/mediawiki/2018/f/f5/T--UPF_CRG_Barcelona--registryparts.svg" alt=""> | ||
<p>Parts Registry</p> | <p>Parts Registry</p> | ||
Latest revision as of 00:41, 18 October 2018
CONSTRUCTION INTERMEDIATES
Bba_K2581000: pFadBA with weak RBS and double terminator
It is the construction intermediate used in the BBa_K2581004 part. It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_K817002), and a weak RBS (BBa_B0032).
BBa_K2581001: pFadBA with weak RBS and double terminator
This is the construction intermediate used in the BBa_K2581005 part. It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_K817002), and a weak RBS (BBa_B0033).
BBa_K2581002: pFadBA with strong RBS and double terminator
This is the construction intermediate used in the BBa_K2581006 part. It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_K817002), and a strong RBS (BBa_B0034).
BBa_K2581003: FadD with weak RBS
This is the construction intermediate used in the BBa_K2581009 part. It consists in the assembly of the weak RBS 32 (BBa_B0032) and the FadD gene (BBa_K2581016) obtained of the own E. coli DH5-alpha strain genome.
BBa_K2581013: pAR: Improved fatty acyl-CoA responsive promoter
The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty acid intracellular promoter pFadBA (BBa_k817002). This new promoter has two binding sites for FadR in comparison with the existing part. Therefore, we have demonstrate an enhanced function of this promoter resulting in a disminution of basility and hence, an improvement of this part. This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), and the improved promoter based on the previous pFadBA DNA sequence.
BBa_K2581017: Intermediate fatty acid biosensor with sharp response
The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid uptake consisting in a high pass filter in order to reduce the high basal activity of the promoter in another efficient way. It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_K817002), a strong RBS (BBa_B0034), a CI repressor from E. coli phage lambda (+ LVA) (BBa_C0051), again the double terminator (BBa_B0014), a modified lambda Prm promoter (BBa_I12036), again the strong RBS (BBa_B0034) and an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).
BBa_K2581018: Intermediate fatty acid biosensor with sharp response
The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid uptake consisting in a high pass filter in order to reduce the high basal activity of the promoter in another efficient way. It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_K817002), a weak RBS (BBa_B0032), a CI repressor from E. coli phage lambda (+LVA) (BBa_C0051), again the double terminator (BBa_B0014), a modified lambda Prm promoter (BBa_I12036), again the weak RBS (BBa_B0032), and an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).
