Difference between revisions of "Team:East Chapel Hill/Results"

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<h1>Results</h1>
 
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<p>Here you can describe the results of your project and your future plans. </p>
 
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<h3>What should this page contain?</h3>
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<li> Clearly and objectively describe the results of your work.</li>
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<li> Future plans for the project. </li>
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<li> Considerations for replicating the experiments. </li>
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<h3>Describe what your results mean </h3>
 
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
 
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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
 
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
 
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<h1>Results</h1>
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<p2> Due to experimental errors and lack of reproducibility, there is some uncertainty in several of our plating assays. However, the results from August 22nd, 2018 appear to be the most accurate due to the lack of growth of the fluoride-binding mutant. Upon looking at these results, we can reasonably infer that the promoter J23_102 and the riboswitch variation ‘FRS1’ are most effective in allowing for dynamic bacterial growth across varied levels of fluoride. </p2>
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<img src="https://static.igem.org/mediawiki/2018/9/98/T--East_Chapel_Hill--RESULTSPAGE1.png" </img>
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<img src="https://static.igem.org/mediawiki/2018/0/08/T--East_Chapel_Hill--RESULTSPAGE2.png" </img>
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<br> <br>
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<h1> Future Directions </h1>
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<p2>
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<br>Short Term:
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<br><br> - We hope to repeat our plating assays to gain more conclusive results in terms of ensuring a direct correlation between the fluoride concentration and bacterial growth
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<br><br> - The CHOP system can be used to screen and select fluoride riboswitches with a higher affinity for fluoride, leading to more growth at lower levels of fluoride and more effective detection. This would also make the system more valuable for implementation in communities where a fluoride detection system is most needed.
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<br><br> - We hope to test the effectiveness of a liquid culture for our construct. Different riboswitch promoter pairs can be inserted into CHOP and response to varying levels of fluoride can be measured.
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<h3> Project Achievements </h3>
 
  
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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Long Term:
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<br> <br>  - In order to bioremediate excess levels of fluoride, we envision using fluoride riboswitches to express genes that will allow for enzymes to metabolize fluoride in the body.  
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<br> <br>  - To sequester fluoride we hope to identify fluoride riboswitches with a higher affinity to fluoride and attach multiple copies of the riboswitches to nanoparticles
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<br> <br>  - For easier implementation, we hope to allow riboswitches to regulate a reporter for a more obvious sign when unhealthy levels of fluoride are present in drinking water.  
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<br> <br>  - CHOP can be used to evaluate other transcriptional riboswitches that bind different metal ions, such as cobalt or nickel. 
  
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<li>A list of linked bullet points of the successful results during your project</li>
 
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
 
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<h3>Inspiration</h3>
 
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<p>See how other teams presented their results.</p>
 
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
 
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
 
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
 
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Latest revision as of 00:42, 18 October 2018

East Chapel Hill

Results

Due to experimental errors and lack of reproducibility, there is some uncertainty in several of our plating assays. However, the results from August 22nd, 2018 appear to be the most accurate due to the lack of growth of the fluoride-binding mutant. Upon looking at these results, we can reasonably infer that the promoter J23_102 and the riboswitch variation ‘FRS1’ are most effective in allowing for dynamic bacterial growth across varied levels of fluoride.


Future Directions


Short Term:

- We hope to repeat our plating assays to gain more conclusive results in terms of ensuring a direct correlation between the fluoride concentration and bacterial growth

- The CHOP system can be used to screen and select fluoride riboswitches with a higher affinity for fluoride, leading to more growth at lower levels of fluoride and more effective detection. This would also make the system more valuable for implementation in communities where a fluoride detection system is most needed.

- We hope to test the effectiveness of a liquid culture for our construct. Different riboswitch promoter pairs can be inserted into CHOP and response to varying levels of fluoride can be measured.

Long Term:

- In order to bioremediate excess levels of fluoride, we envision using fluoride riboswitches to express genes that will allow for enzymes to metabolize fluoride in the body.

- To sequester fluoride we hope to identify fluoride riboswitches with a higher affinity to fluoride and attach multiple copies of the riboswitches to nanoparticles

- For easier implementation, we hope to allow riboswitches to regulate a reporter for a more obvious sign when unhealthy levels of fluoride are present in drinking water.

- CHOP can be used to evaluate other transcriptional riboswitches that bind different metal ions, such as cobalt or nickel.