Difference between revisions of "Team:Munich/growth.html"

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<html>
 
<div class="event-info">
 
<div class="event-info">
  
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<h4>Growth of msbB and RecBCD-Nuklease Deficient E.Coli Mutant Strains</h4>
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<em>2018/08/28</em>
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<table class="table table-borderless">
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    <tr>
 +
 +
      <td>Participants:</td>
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      <td>Katja Neishsalo</td>
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    </tr>
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    <tr>
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      <td>Protocol:</td>
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      <td>Measuring optical density</td>
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    </tr>
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    <tr>
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      <td>Notes:</td>
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      <td> Strains tested: deltamsbB; DeltaRecB, DeltaRecD and WT (MG1655); inoculation of 100 ml LB medium with 1 ml preculture. Measure OD every 30 – 60 minutes.
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 +
</td>
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    </tr>
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<tr>
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      <td>Results:</td>
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      <td><figure class="figure">
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  <img src="https://static.igem.org/mediawiki/2018/3/31/T--munich--Growth_curves.jpg " class="figure-img img-fluid rounded" alt=" ">
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    <figcaption class="figure-caption"></figcaption>
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    </figure>
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</td>
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    </tr>
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</table>
  
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<h3>8/28</h3>
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<h4>Preparing Electro-Competent Cells of WT RecBCD und dRecB</h4>
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<h4>growth of msbB and Recombinase deficient E.Coli mutant strains</h4>
+
<em>2018/08/31</em>
 
<table class="table table-borderless">
 
<table class="table table-borderless">
 
     <tr>
 
     <tr>
  
 
       <td>Participants:</td>
 
       <td>Participants:</td>
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       <td>katja</td>
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       <td>Katja Neishsalo</td>
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    </tr>
 +
    <tr>
 +
      <td>Protocol:</td>
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      <td>
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<a href="https://www.neb.com/protocols/2012/06/21/making-your-own-electrocompetent-cells" target="_blank">Preparation electrocompetent cells</a>
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</td>
 +
    </tr>
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<tr>
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      <td>Results:</td>
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      <td> PICcells are still viable after preparation, dRecB grows significantly slower than others</td>
 +
    </tr>
 +
</table>
 +
 
 +
<h4>Transforming E. Coli dRecB with pSB1C3_RecBCD(WT)</h4>
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<em>2018/09/03</em>
 +
<table class="table table-borderless">
 +
    <tr>
 +
 
 +
      <td>Participants:</td>
 +
      <td>Katja Neishsalo</td>
 +
    </tr>
 +
    <tr>
 +
      <td>Protocol:</td>
 +
      <td>
 +
<a href="https://static.igem.org/mediawiki/2018/c/ca/T--Munich--WL1_Electrocompetent_transformation.pdf" target="_blank">Electrocompetent transformation</a>
 +
</td>
 +
    </tr>
 +
<tr>
 +
      <td>Results:</td>
 +
      <td>Colonies</td>
 +
    </tr>
 +
</table>
 +
 
 +
<h4>DNA Midi Prep of RecBCD(WT) Plasmid from Dh5a</h4>
 +
<em>2018/09/03</em>
 +
<table class="table table-borderless">
 +
    <tr>
 +
 
 +
      <td>Participants:</td>
 +
      <td>Katja Neishsalo</td>
 +
    </tr>
 +
    <tr>
 +
      <td>Protocol:</td>
 +
      <td>Midiprep</td>
 +
    </tr>
 +
</table>
 +
 
 +
<h4>Growth of RecB, RecD, RecBCD, msb�-B Deficient E.Coli Strains</h4>
 +
<em>2018/09/14</em>
 +
<table class="table table-borderless">
 +
    <tr>
 +
      <td>Participants:</td>
 +
      <td>Katja Neishsalo</td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
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  <tr>
 
  <tr>
 
       <td>Results:</td>
 
       <td>Results:</td>
−
       <td>PIC</td>
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       <td><figure class="figure">
 +
  <img src="https://static.igem.org/mediawiki/2018/c/c6/T--munich--growth_curves_katja-2.jpg  " class="figure-img img-fluid rounded" alt=" ">
 +
    <figcaption class="figure-caption">The experiment shows no significant difference between growth curves of KO strains compared to those with RecBCDwt-Plasmid. The reference curves (strains transformed with mRFP) have the highest OD values. We suppose that mRFP falsified the OD values since it also emits fluorescence in the wavelength of the OD measurement.</figcaption>
 +
    </figure>
 +
</td>
 
     </tr>
 
     </tr>
 
</table>
 
</table>
  
 
</div>
 
</div>
 +
</html>

Latest revision as of 00:43, 18 October 2018

Growth of msbB and RecBCD-Nuklease Deficient E.Coli Mutant Strains

2018/08/28
Participants: Katja Neishsalo
Protocol: Measuring optical density
Notes: Strains tested: deltamsbB; DeltaRecB, DeltaRecD and WT (MG1655); inoculation of 100 ml LB medium with 1 ml preculture. Measure OD every 30 – 60 minutes.
Results:

Preparing Electro-Competent Cells of WT RecBCD und dRecB

2018/08/31
Participants: Katja Neishsalo
Protocol: Preparation electrocompetent cells
Results: PICcells are still viable after preparation, dRecB grows significantly slower than others

Transforming E. Coli dRecB with pSB1C3_RecBCD(WT)

2018/09/03
Participants: Katja Neishsalo
Protocol: Electrocompetent transformation
Results: Colonies

DNA Midi Prep of RecBCD(WT) Plasmid from Dh5a

2018/09/03
Participants: Katja Neishsalo
Protocol: Midiprep

Growth of RecB, RecD, RecBCD, msb�-B Deficient E.Coli Strains

2018/09/14
Participants: Katja Neishsalo
Protocol: Measuring optical density
Notes:
Results:
The experiment shows no significant difference between growth curves of KO strains compared to those with RecBCDwt-Plasmid. The reference curves (strains transformed with mRFP) have the highest OD values. We suppose that mRFP falsified the OD values since it also emits fluorescence in the wavelength of the OD measurement.