Difference between revisions of "Team:Munich/growth.html"
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<div class="event-info"> | <div class="event-info"> | ||
| − | + | <h4>Growth of msbB and RecBCD-Nuklease Deficient E.Coli Mutant Strains</h4> | |
| − | + | <em>2018/08/28</em> | |
| − | <h4> | + | |
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
<tr> | <tr> | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td> | + | <td>Katja Neishsalo</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 16: | Line 16: | ||
<tr> | <tr> | ||
<td>Notes:</td> | <td>Notes:</td> | ||
| − | <td> | + | <td> Strains tested: deltamsbB; DeltaRecB, DeltaRecD and WT (MG1655); inoculation of 100 ml LB medium with 1 ml preculture. Measure OD every 30 – 60 minutes. |
| + | |||
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Results:</td> | <td>Results:</td> | ||
| − | <td> | + | <td><figure class="figure"> |
| + | <img src="https://static.igem.org/mediawiki/2018/3/31/T--munich--Growth_curves.jpg " class="figure-img img-fluid rounded" alt=" "> | ||
| + | <figcaption class="figure-caption"></figcaption> | ||
| + | </figure> | ||
| + | </td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
| − | + | <h4>Preparing Electro-Competent Cells of WT RecBCD und dRecB</h4> | |
| − | <h4> | + | <em>2018/08/31</em> |
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
<tr> | <tr> | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td> | + | <td>Katja Neishsalo</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td> | + | <td> |
| + | <a href="https://www.neb.com/protocols/2012/06/21/making-your-own-electrocompetent-cells" target="_blank">Preparation electrocompetent cells</a> | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Results:</td> | <td>Results:</td> | ||
| − | <td> | + | <td> PICcells are still viable after preparation, dRecB grows significantly slower than others</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| − | + | <h4>Transforming E. Coli dRecB with pSB1C3_RecBCD(WT)</h4> | |
| − | <h4>Transforming E.Coli dRecB with pSB1C3_RecBCD(WT)</h4> | + | <em>2018/09/03</em> |
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
<tr> | <tr> | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td> | + | <td>Katja Neishsalo</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | |||
| − | |||
| − | |||
| − | |||
<td> | <td> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/c/ca/T--Munich--WL1_Electrocompetent_transformation.pdf" target="_blank">Electrocompetent transformation</a> | ||
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Results:</td> | <td>Results:</td> | ||
| − | <td> | + | <td>Colonies</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| − | + | <h4>DNA Midi Prep of RecBCD(WT) Plasmid from Dh5a</h4> | |
| − | <h4>DNA | + | <em>2018/09/03</em> |
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
<tr> | <tr> | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td> | + | <td>Katja Neishsalo</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td> | + | <td>Midiprep</td> |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</tr> | </tr> | ||
</table> | </table> | ||
| − | + | <h4>Growth of RecB, RecD, RecBCD, msb�-B Deficient E.Coli Strains</h4> | |
| − | <h4> | + | <em>2018/09/14</em> |
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
<tr> | <tr> | ||
| − | |||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td> | + | <td>Katja Neishsalo</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 108: | Line 102: | ||
<tr> | <tr> | ||
<td>Results:</td> | <td>Results:</td> | ||
| − | <td> | + | <td><figure class="figure"> |
| + | <img src="https://static.igem.org/mediawiki/2018/c/c6/T--munich--growth_curves_katja-2.jpg " class="figure-img img-fluid rounded" alt=" "> | ||
| + | <figcaption class="figure-caption">The experiment shows no significant difference between growth curves of KO strains compared to those with RecBCDwt-Plasmid. The reference curves (strains transformed with mRFP) have the highest OD values. We suppose that mRFP falsified the OD values since it also emits fluorescence in the wavelength of the OD measurement.</figcaption> | ||
| + | </figure> | ||
| + | </td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
</div> | </div> | ||
| + | </html> | ||
Latest revision as of 00:43, 18 October 2018
Growth of msbB and RecBCD-Nuklease Deficient E.Coli Mutant Strains
2018/08/28| Participants: | Katja Neishsalo |
| Protocol: | Measuring optical density |
| Notes: | Strains tested: deltamsbB; DeltaRecB, DeltaRecD and WT (MG1655); inoculation of 100 ml LB medium with 1 ml preculture. Measure OD every 30 – 60 minutes. |
| Results: | 
|
Preparing Electro-Competent Cells of WT RecBCD und dRecB
2018/08/31| Participants: | Katja Neishsalo |
| Protocol: | Preparation electrocompetent cells |
| Results: | PICcells are still viable after preparation, dRecB grows significantly slower than others |
Transforming E. Coli dRecB with pSB1C3_RecBCD(WT)
2018/09/03| Participants: | Katja Neishsalo |
| Protocol: | Electrocompetent transformation |
| Results: | Colonies |
DNA Midi Prep of RecBCD(WT) Plasmid from Dh5a
2018/09/03| Participants: | Katja Neishsalo |
| Protocol: | Midiprep |
Growth of RecB, RecD, RecBCD, msb�-B Deficient E.Coli Strains
2018/09/14| Participants: | Katja Neishsalo |
| Protocol: | Measuring optical density |
| Notes: | |
| Results: | 
|