Difference between revisions of "Team:UPF CRG Barcelona/Composite"
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<link rel="stylesheet" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/styles?action=raw&ctype=text/css" type='text/css'> | <link rel="stylesheet" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/styles?action=raw&ctype=text/css" type='text/css'> | ||
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<body> | <body> | ||
<div class="sticky-top"> | <div class="sticky-top"> | ||
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<div class="navbar-brand"> | <div class="navbar-brand"> | ||
| − | <a class="navbar-logo" href=" | + | <a class="navbar-logo" href="https://2018.igem.org/Team:UPF_CRG_Barcelona"> |
| − | <img src=" | + | <img src="https://static.igem.org/mediawiki/2018/3/37/T--UPF_CRG_Barcelona--logosensebarcelona.svg" alt="iGEM Barcelona team 2018"> |
</a> | </a> | ||
<div class="navbar-burger" data-target="navbar-menu"> | <div class="navbar-burger" data-target="navbar-menu"> | ||
| Line 23: | Line 24: | ||
<div class="navbar-grid"> | <div class="navbar-grid"> | ||
<!--class is-active for showing active menu--> | <!--class is-active for showing active menu--> | ||
| − | <a class="navbar-item" href=" | + | <a class="navbar-item" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Description">Project</a> |
| − | <a class="navbar-item" href="DryLab.html">DryLab</a> | + | <a class="navbar-item" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/DryLab.html">DryLab</a> |
| − | <a class="navbar-item is-active" href=" | + | <a class="navbar-item is-active" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Parts">Parts</a> |
| − | <a class="navbar-item" href=" | + | <a class="navbar-item" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Deliverables">Deliverables</a> |
| − | <a class="navbar-item" href=" | + | <a class="navbar-item" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/WetLab">WetLab</a> |
| − | <a class="navbar-item" href=" | + | <a class="navbar-item" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Intro_HP">Human Practices</a> |
| − | <a class="navbar-item" href=" | + | <a class="navbar-item" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Aboutteam">Team</a> |
</div> | </div> | ||
</div> | </div> | ||
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<div class="swiper-container" id="sw-container"> | <div class="swiper-container" id="sw-container"> | ||
<div class="swiper-wrapper"> | <div class="swiper-wrapper"> | ||
| − | <a class="swiper-slide swiper-slide-menu" href=" | + | <a class="swiper-slide swiper-slide-menu" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Basic"> |
| − | <embed src=" | + | <embed src="https://static.igem.org/mediawiki/2018/2/25/T--UPF_CRG_Barcelona--basicpart.svg" alt=""> |
<p>Basic Parts</p> | <p>Basic Parts</p> | ||
</a> | </a> | ||
| − | <a class="swiper-slide swiper-slide-menu" href=" | + | <a class="swiper-slide swiper-slide-menu" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Intermediate"> |
| − | < | + | <embed src="https://2018.igem.org//wiki/images/e/e8/T--UPF_CRG_Barcelona--partsdeliverables_navbar.svg" alt=""> |
<p>Construction<br>Intermediates</p> | <p>Construction<br>Intermediates</p> | ||
</a> | </a> | ||
| − | <a class="swiper-slide swiper-slide-menu" href=" | + | <a class="swiper-slide swiper-slide-menu" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Composite"> |
| − | <embed src=" | + | <embed src="https://static.igem.org/mediawiki/2018/e/ed/T--UPF_CRG_Barcelona--compositepart.svg" alt=""> |
<p>Composite Parts</p> | <p>Composite Parts</p> | ||
</a> | </a> | ||
| − | <a class="swiper-slide swiper-slide-menu" href="Improve | + | <a class="swiper-slide swiper-slide-menu" href="Improve"> |
| − | <embed src=" | + | <embed src="https://static.igem.org/mediawiki/2018/9/96/T--UPF_CRG_Barcelona--improvedpart.svg" alt=""> |
<p>Improved Parts</p> | <p>Improved Parts</p> | ||
</a> | </a> | ||
| − | <a class="swiper-slide swiper-slide-menu" href=" | + | <a class="swiper-slide swiper-slide-menu" href="https://2018.igem.org/Team:UPF_CRG_Barcelona/Registry"> |
| − | <embed src=" | + | <embed src="https://static.igem.org/mediawiki/2018/f/f5/T--UPF_CRG_Barcelona--registryparts.svg" alt=""> |
<p>Parts Registry</p> | <p>Parts Registry</p> | ||
</a> | </a> | ||
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<div> | <div> | ||
<p class="page-title">COMPOSITE PARTS</p> | <p class="page-title">COMPOSITE PARTS</p> | ||
| − | <p><b>BBa_K2581004: | + | <p><b>BBa_K2581004: Fatty acid acyl-CoA biosensor with weak RBS</b></p> |
<p>The UPF_CRG_Barcelona iGEM team 2018 has created this part as an intracellular biosensor of fatty acid | <p>The UPF_CRG_Barcelona iGEM team 2018 has created this part as an intracellular biosensor of fatty acid | ||
uptake. It consists of the assembly of a double terminator which allows forward and reverse terminator | uptake. It consists of the assembly of a double terminator which allows forward and reverse terminator | ||
| Line 81: | Line 82: | ||
fluorescent protein from <i>Discosoma striata</i> (BBa_E1010). </p> | fluorescent protein from <i>Discosoma striata</i> (BBa_E1010). </p> | ||
<center><img src="https://static.igem.org/mediawiki/2018/c/c1/T--UPF_CRG_Barcelona--partpFadBA32.svg" style="width: 45%;"></center> | <center><img src="https://static.igem.org/mediawiki/2018/c/c1/T--UPF_CRG_Barcelona--partpFadBA32.svg" style="width: 45%;"></center> | ||
| − | <p><b>BBa_K2581005: | + | <p><b>BBa_K2581005: Fatty acid acyl-CoA biosensor with weak RBS</b></p> |
<p>The UPF_CRG_Barcelona iGEM team 2018 has created this part as an intracellular biosensor of fatty acid | <p>The UPF_CRG_Barcelona iGEM team 2018 has created this part as an intracellular biosensor of fatty acid | ||
uptake. It consists of the assembly of a double terminator which allows forward and reverse terminator | uptake. It consists of the assembly of a double terminator which allows forward and reverse terminator | ||
| Line 88: | Line 89: | ||
fluorescent protein from <i>Discosoma striata</i> (BBa_E1010). </p> | fluorescent protein from <i>Discosoma striata</i> (BBa_E1010). </p> | ||
<center><img src="https://static.igem.org/mediawiki/2018/6/6a/T--UPF_CRG_Barcelona--partpFadBA33.svg" style="width: 45%;"></center> | <center><img src="https://static.igem.org/mediawiki/2018/6/6a/T--UPF_CRG_Barcelona--partpFadBA33.svg" style="width: 45%;"></center> | ||
| − | <p><b>BBa_K2581006: | + | <p><b>BBa_K2581006: Fatty acid acyl-CoA biosensor with strong RBS</b></p> |
<p>The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid | <p>The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid | ||
uptake. It consists of the assembly of a double terminator which allows forward and reverse terminator | uptake. It consists of the assembly of a double terminator which allows forward and reverse terminator | ||
| Line 95: | Line 96: | ||
fluorescent protein from <i>Discosoma striata</i> (BBa_E1010). </p> | fluorescent protein from <i>Discosoma striata</i> (BBa_E1010). </p> | ||
<center><img src="https://static.igem.org/mediawiki/2018/b/bc/T--UPF_CRG_Barcelona--partpFadBA34.svg" style="width: 45%;"></center> | <center><img src="https://static.igem.org/mediawiki/2018/b/bc/T--UPF_CRG_Barcelona--partpFadBA34.svg" style="width: 45%;"></center> | ||
| − | <p><b>BBa_K2581007: | + | <p><b>BBa_K2581007: Inducible LuxR-pLux engineered device with weak RBS</b></p> |
<p>The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid | <p>The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid | ||
uptake. Considering the leakage of the promoter being used a LuxR/pluxR lactone inducible system was coupled | uptake. Considering the leakage of the promoter being used a LuxR/pluxR lactone inducible system was coupled | ||
| Line 108: | Line 109: | ||
<center><img src="https://static.igem.org/mediawiki/2018/3/34/T--UPF_CRG_Barcelona--partlux34.svg" style="width: 50%;"></center> | <center><img src="https://static.igem.org/mediawiki/2018/3/34/T--UPF_CRG_Barcelona--partlux34.svg" style="width: 50%;"></center> | ||
| − | <p><b>BBa_K2581008: | + | <p><b>BBa_K2581008: Inducible LuxR-pLux engineered device with strong RBS</b></p> |
<p>The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid | <p>The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid | ||
uptake. Considering the leakage of the promoter being used a LuxR/pluxR lactone inducible system was coupled | uptake. Considering the leakage of the promoter being used a LuxR/pluxR lactone inducible system was coupled | ||
| Line 119: | Line 120: | ||
<center><img src="https://static.igem.org/mediawiki/2018/7/76/T--UPF_CRG_Barcelona--partlux3434.svg" style="width: 50%;"></center> | <center><img src="https://static.igem.org/mediawiki/2018/7/76/T--UPF_CRG_Barcelona--partlux3434.svg" style="width: 50%;"></center> | ||
| − | <p><b>BBa_K2581009: | + | <p><b>BBa_K2581009: FadD gene with ATc inducible promoter with weak RBS</b></p> |
<p>The UPF_CRG_Barcelona iGEM team 2018 has created this part which consist of the expression of the own <i>E. | <p>The UPF_CRG_Barcelona iGEM team 2018 has created this part which consist of the expression of the own <i>E. | ||
coli</i> acyl-CoA Synthetase gene, FadD. This gene has an essential role in the fatty acid transportation | coli</i> acyl-CoA Synthetase gene, FadD. This gene has an essential role in the fatty acid transportation | ||
| Line 128: | Line 129: | ||
<center><img src="https://static.igem.org/mediawiki/2018/9/94/T--UPF_CRG_Barcelona--partfadDcomposite.svg" style="width: 45%;"></center> | <center><img src="https://static.igem.org/mediawiki/2018/9/94/T--UPF_CRG_Barcelona--partfadDcomposite.svg" style="width: 45%;"></center> | ||
| − | <p><b>BBa_K2581010: | + | <p><b>BBa_K2581010: FadL gene with ATc inducible promoter with weak RBS</b></p> |
<p>The UPF_CRG_Barcelona iGEM team 2018 has created this part which consist in the expression of the own <i>E. | <p>The UPF_CRG_Barcelona iGEM team 2018 has created this part which consist in the expression of the own <i>E. | ||
coli</i> outer membrane transporter FadL. This gene has an essential role in the fatty acid transportation | coli</i> outer membrane transporter FadL. This gene has an essential role in the fatty acid transportation | ||
| Line 136: | Line 137: | ||
obtained of the own <i>E. coli</i> DH5-alpha strain genome.</p> | obtained of the own <i>E. coli</i> DH5-alpha strain genome.</p> | ||
<center><img src="https://static.igem.org/mediawiki/2018/b/b3/T--UPF_CRG_Barcelona--partfadLcomposite.svg" style="width: 45%;"></center> | <center><img src="https://static.igem.org/mediawiki/2018/b/b3/T--UPF_CRG_Barcelona--partfadLcomposite.svg" style="width: 45%;"></center> | ||
| − | <p><b>BBa_K2581019: | + | <p><b>BBa_K2581019: FadE gene with ATc inducible promoter with weak RBS</b></p> |
<p>The UPF_CRG_Barcelona iGEM team 2018 has created this part which consist in the expression of the acyl-coA | <p>The UPF_CRG_Barcelona iGEM team 2018 has created this part which consist in the expression of the acyl-coA | ||
dehydrogenase (FadE) gene which converts acyl-coA into enoyl-coA.</p> | dehydrogenase (FadE) gene which converts acyl-coA into enoyl-coA.</p> | ||
Latest revision as of 00:43, 18 October 2018
COMPOSITE PARTS
BBa_K2581004: Fatty acid acyl-CoA biosensor with weak RBS
The UPF_CRG_Barcelona iGEM team 2018 has created this part as an intracellular biosensor of fatty acid uptake. It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_k817002), a weak RBS (BBa_B0032) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).
BBa_K2581005: Fatty acid acyl-CoA biosensor with weak RBS
The UPF_CRG_Barcelona iGEM team 2018 has created this part as an intracellular biosensor of fatty acid uptake. It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_k817002), a weak RBS (BBa_B0032) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).
BBa_K2581006: Fatty acid acyl-CoA biosensor with strong RBS
The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid uptake. It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_K817002), a strong RBS (BBa_B0034) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).
BBa_K2581007: Inducible LuxR-pLux engineered device with weak RBS
The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid uptake. Considering the leakage of the promoter being used a LuxR/pluxR lactone inducible system was coupled in order to reduce high basal activity.
It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_k817002), a strong RBS (BBa_B0034), luxR repressor/activator (BBa_C0062), the double terminator again (BBa_B0015), pLux promoter (BBa_R0062) with a weak RBS (BBa_B0032) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).
BBa_K2581008: Inducible LuxR-pLux engineered device with strong RBS
The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid uptake. Considering the leakage of the promoter being used a LuxR/pluxR lactone inducible system was coupled in order to reduce high basal activity.
It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_k817002), a strong RBS (BBa_B0034), luxR repressor/activator (BBa_C0062) the double terminator again (BBa_B0015), pLux promoter (BBa_R0062) with a weak RBS (BBa_B0034) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).
BBa_K2581009: FadD gene with ATc inducible promoter with weak RBS
The UPF_CRG_Barcelona iGEM team 2018 has created this part which consist of the expression of the own E. coli acyl-CoA Synthetase gene, FadD. This gene has an essential role in the fatty acid transportation inside the cell, as well as fatty acid activation. This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a tetR repressible promoter (BBa_R0040), a weak RBS (BBa_B0032) and the the FadD gene (BBa_K258101) obtained of the own E. coli DH5-alpha strain genome.
BBa_K2581010: FadL gene with ATc inducible promoter with weak RBS
The UPF_CRG_Barcelona iGEM team 2018 has created this part which consist in the expression of the own E. coli outer membrane transporter FadL. This gene has an essential role in the fatty acid transportation across the cell membrane via a transportation/acyl-activation mechanism. This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a tetR repressible promoter (BBa_R0040), a weak RBS (BBa_B0032) and the FadL gene (BBa_K2581015) obtained of the own E. coli DH5-alpha strain genome.
BBa_K2581019: FadE gene with ATc inducible promoter with weak RBS
The UPF_CRG_Barcelona iGEM team 2018 has created this part which consist in the expression of the acyl-coA dehydrogenase (FadE) gene which converts acyl-coA into enoyl-coA.
This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a tetR repressible promoter (BBa_R0040), a weak RBS (BBa_B0032) and the FadE gene obtained of the own E. coli DH5-alpha strain genome (BBa_K2581014).
