Difference between revisions of "Team:UPF CRG Barcelona/Basic"
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<div class="navbar-brand"> | <div class="navbar-brand"> | ||
| − | <a class="navbar-logo" href=" | + | <a class="navbar-logo" href="https://2018.igem.org/Team:UPF_CRG_Barcelona"> |
<img src="https://static.igem.org/mediawiki/2018/3/37/T--UPF_CRG_Barcelona--logosensebarcelona.svg" alt="iGEM Barcelona team 2018"> | <img src="https://static.igem.org/mediawiki/2018/3/37/T--UPF_CRG_Barcelona--logosensebarcelona.svg" alt="iGEM Barcelona team 2018"> | ||
</a> | </a> | ||
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<div> | <div> | ||
<p class="page-title">BASIC PARTS</p> | <p class="page-title">BASIC PARTS</p> | ||
| − | <p><b>BBa_K2581014: FadE</b></p> | + | <p><b>BBa_K2581014: FadE gene</b></p> |
<p>The UPF_CRG_Barcelona iGEM team 2018 has synthesized this part as a result of a pcr amplification from the | <p>The UPF_CRG_Barcelona iGEM team 2018 has synthesized this part as a result of a pcr amplification from the | ||
DH5-alpha <i>E. coli</i> strain. It consists of the coding sequence of the acyl-coA dehydrogenase (FadE) gene | DH5-alpha <i>E. coli</i> strain. It consists of the coding sequence of the acyl-coA dehydrogenase (FadE) gene | ||
which converts acyl-coA into enoyl-coA. This part is a twin of the BBa_K861020). </p> | which converts acyl-coA into enoyl-coA. This part is a twin of the BBa_K861020). </p> | ||
<center><img src="https://static.igem.org/mediawiki/2018/8/8d/T--UPF_CRG_Barcelona--partFadE.svg" style="width: 35%;"></center> | <center><img src="https://static.igem.org/mediawiki/2018/8/8d/T--UPF_CRG_Barcelona--partFadE.svg" style="width: 35%;"></center> | ||
| − | <p><b>BBa_K2581015: FadL</b></p> | + | <p><b>BBa_K2581015: FadL gene</b></p> |
<p>The UPF_CRG_Barcelona iGEM team 2018 has synthesized this part as a result of a pcr amplification from the | <p>The UPF_CRG_Barcelona iGEM team 2018 has synthesized this part as a result of a pcr amplification from the | ||
DH5-alpha <i>E. coli</i> strain. It consists of the coding sequence of the transmembrane protein FadL. This | DH5-alpha <i>E. coli</i> strain. It consists of the coding sequence of the transmembrane protein FadL. This | ||
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<center><img src="https://static.igem.org/mediawiki/2018/d/d8/T--UPF_CRG_Barcelona--partFadL.svg" style="width: 35%;"></center> | <center><img src="https://static.igem.org/mediawiki/2018/d/d8/T--UPF_CRG_Barcelona--partFadL.svg" style="width: 35%;"></center> | ||
| − | <p><b>Bba_K2581016: FadD</b></p> | + | <p><b>Bba_K2581016: FadD gene</b></p> |
<p>The UPF_CRG_Barcelona iGEM team 2018 has synthesized this part as a result of a pcr amplification from the | <p>The UPF_CRG_Barcelona iGEM team 2018 has synthesized this part as a result of a pcr amplification from the | ||
DH5-alpha <i>E. coli</i> strain. It consists of the coding sequence of the acyl-coA synthetase gene FadD. | DH5-alpha <i>E. coli</i> strain. It consists of the coding sequence of the acyl-coA synthetase gene FadD. | ||
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<center><img src="https://static.igem.org/mediawiki/2018/3/36/T--UPF_CRG_Barcelona--partFadD.svg" style="width: 35%;"></center> | <center><img src="https://static.igem.org/mediawiki/2018/3/36/T--UPF_CRG_Barcelona--partFadD.svg" style="width: 35%;"></center> | ||
| − | <p><b>BBa_K2581020: | + | <p><b>BBa_K2581020: Modified fatty acyl-CoA responsive promoter</b></p> |
<p>The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty | <p>The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty | ||
acid intracellular promoter pFadBA (BBa_K817002). This new promoter has two binding sites for FadR in | acid intracellular promoter pFadBA (BBa_K817002). This new promoter has two binding sites for FadR in | ||
Latest revision as of 00:44, 18 October 2018
BASIC PARTS
BBa_K2581014: FadE gene
The UPF_CRG_Barcelona iGEM team 2018 has synthesized this part as a result of a pcr amplification from the DH5-alpha E. coli strain. It consists of the coding sequence of the acyl-coA dehydrogenase (FadE) gene which converts acyl-coA into enoyl-coA. This part is a twin of the BBa_K861020).
BBa_K2581015: FadL gene
The UPF_CRG_Barcelona iGEM team 2018 has synthesized this part as a result of a pcr amplification from the DH5-alpha E. coli strain. It consists of the coding sequence of the transmembrane protein FadL. This gene has an essential role in the fatty acid transportation across the cell membrane via a transportation.
Bba_K2581016: FadD gene
The UPF_CRG_Barcelona iGEM team 2018 has synthesized this part as a result of a pcr amplification from the DH5-alpha E. coli strain. It consists of the coding sequence of the acyl-coA synthetase gene FadD. This gene has an essential role in the fatty acid transportation inside the cell and in acyl-activation mechanism.
BBa_K2581020: Modified fatty acyl-CoA responsive promoter
The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty acid intracellular promoter pFadBA (BBa_K817002). This new promoter has two binding sites for FadR in comparison with the existing part.
