Difference between revisions of "Team:Kyoto/Basic Parts"

 
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<body>
 
<body>
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<div class="clear"></div>
 
<div id="jump">
 
<div id="jump">
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<a href="#wrapper"><img src="https://static.igem.org/mediawiki/2018/b/b5/T--Kyoto--UpButton.png"></a></div>
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  <div id="BACKGROUND">
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<a href="#wrapper">
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  <div id="wrapper"><br><br><br>
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<img src=""></a></div>
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  <h1><font face="Segoe UI">Basic Parts</font></h1>
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<div id="BACKGROUND">
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<div style='padding-top: 100px;'><h1 id="wrapper"><img src="https://static.igem.org/mediawiki/2018/1/1d/T--Kyoto--basicparts.png" width="30%"></div></h1>
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<!-- Table Generated by TableMaker.py Author: OHAD -->
 
<!-- Table Generated by TableMaker.py Author: OHAD -->
−
<h5><font face="Segoe UI" font color=#757575>Basic Parts</font></h5>
+
<h5 id="Basic_Parts">Basic Parts</h5>
 +
 
 +
 
 
<table>
 
<table>
  
 
<tr><th>Number
 
<tr><th>Number
−
</th><th>Parts
+
</th><th width=30%>parts
−
</th><th>Designer
+
</th><th>Remark
−
</th><th>Links
+
</th><th width=8%>Designer
−
</th><th>用意すべき実験データ
+
</th><th>Remark2
−
</th><th>提出
+
</th><th>Subimittion
−
</th><th>ページ編集
+
−
</th><th>備考
+
 
</th></tr>
 
</th></tr>
  
−
<tr><td>BBa_K2665011
+
<tr><td><a href="http://parts.igem.org/Part:BBa_K2665000"><font color="#007081"><b>BBa_K2665000
−
</td><td>TDH3-Mangrin-6xHis-CYC
+
</b></font></a></td><td>TDH3 promoter
 +
</td><td>－
 
</td><td>Tong
 
</td><td>Tong
−
</td><td><a href="">http://parts.igem.org/Part:BBa_K2665011</a>
+
</td><td>－
−
</td><td>スポットアッセイとボイル法
+
</td><td>not submitted
−
</td><td>する、１A
+
 
−
</td><td>あとは実験データのせるだけ
+
</td></tr><tr><td><a href=" http://parts.igem.org/Part:BBa_K2665001"><font color="#007081"><b>BBa_K2665001
−
</td><td>なし
+
</b></font></a></td><td>CYCterm fused with 6xHis tag and stop codon
−
</td></tr><tr><td>BBa_K2665012
+
</td><td>－
−
</td><td>TDH3-ZrGPD1-6xHis-CYC
+
 
</td><td>Tong
 
</td><td>Tong
−
</td><td><a href="">http://parts.igem.org/Part:BBa_K2665012</a>
+
</td><td>－
−
</td><td>〃
+
</td><td>not submitted
−
</td><td>する１B
+
 
−
</td><td>〃
+
</td></tr><tr><td><a href=" http://parts.igem.org/Part:BBa_K2665002"><font color="#007081"><b>BBa_K2665002
−
</td><td>なし
+
</b></font></a></td><td>Mangrin ORF
−
</td></tr><tr><td>BBa_K2665013
+
</td><td>－
−
</td><td>TDH3-AtNHXS1-6xHis-CYC
+
 
</td><td>Tong
 
</td><td>Tong
−
</td><td><a href="">http://parts.igem.org/Part:BBa_K2665013</a>
+
</td><td>－
−
</td><td>〃
+
</td><td>not submitted
−
</td><td>する１C
+
 
−
</td><td>〃
+
</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665003"><font color="#007081"><b>BBa_K2665003
−
</td><td>なし
+
</b></font></a></td><td>ZrGPD1 ORF
−
</td></tr><tr><td>BBa_K2665014
+
</td><td>－
−
</td><td>TDH3-SseNHX1-6xHis-CYC
+
 
</td><td>Tong
 
</td><td>Tong
−
</td><td><a href="">http://parts.igem.org/Part:BBa_K2665014</a>
+
</td><td>－
−
</td><td>〃
+
</td><td>not submitted
−
</td><td>する１E
+
 
−
</td><td>未完
+
 
−
</td><td>codon optimized
+
</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665005"><font color="#007081"><b>BBa_K2665005
−
</td></tr><tr><td>BBa_K2665015
+
</b></font></a></td><td>SseNHX1 ORF
−
</td><td>TDH3-AVP1-6xHis-CYC
+
</td><td>－
 
</td><td>Tong
 
</td><td>Tong
−
</td><td><a href="">http://parts.igem.org/Part:BBa_K2665015</a>
+
</td><td>－
−
</td><td>〃
+
</td><td>not submitted
−
</td><td>する１D
+
 
−
</td><td>未完
+
</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665006"><font color="#007081"><b>BBa_K2665006
−
</td><td>終止コドン消してないことを書く
+
</b></font></a></td><td>AVP1 ORF
−
</td></tr><tr><td>BBa_K2665016
+
</td><td>including the stop codon
−
</td><td>TDH3-AtHKT1-6xHis-CYC
+
 
</td><td>Tong
 
</td><td>Tong
−
</td><td><a href="">http://parts.igem.org/Part:BBa_K2665016</a>
+
</td><td>－
−
</td><td>〃
+
</td><td>not submitted
−
</td><td>する１F
+
 
−
</td><td>未完
+
</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665007"><font color="#007081"><b>BBa_K2665007
−
</td><td>なし
+
</b></font></a></td><td>AtHKT1 ORF
−
</td></tr><tr><td>BBa_K2665017
+
</td><td>－
−
</td><td>TDH3-ZrFPS1-6xHis-CYC
+
 
</td><td>Tong
 
</td><td>Tong
−
</td><td><a href="">http://parts.igem.org/Part:BBa_K2665017</a>
+
</td><td>－
−
</td><td>〃
+
</td><td>not submitted
−
</td><td>しない
+
 
−
</td><td>未完
+
</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665008"><font color="#007081"><b>BBa_K2665008
−
</td><td>なし
+
</b></font></a></td><td>ZrFPS1 ORF
−
</td></tr><tr><td>
+
</td><td>－
−
</td><td>
+
−
</td><td>
+
−
</td><td><a href=""></a>
+
−
</td><td>
+
−
</td><td>
+
−
</td><td>
+
−
</td><td>
+
−
</td></tr><tr><td>BBa_K2665018
+
−
</td><td>Fgβ
+
 
</td><td>Tong
 
</td><td>Tong
−
</td><td><a href="">http://parts.igem.org/Part:BBa_K2665018</a>
+
</td><td>－
−
</td><td>蛍光顕微鏡
+
</td><td>not submitted
−
</td><td>する2A
+
 
−
</td><td>未完
+
 
−
</td><td>なし
+
</td></tr><tr><td><a href=" http://parts.igem.org/Part:BBa_K2665018"><font color="#007081"><b>BBa_K2665018
−
</td></tr><tr><td>BBa_K2665019
+
</b></font></a></td><td>Fgb
−
</td><td>SdrG
+
</td><td>－
 
</td><td>Tong
 
</td><td>Tong
−
</td><td><a href=""></a>
+
</td><td>Fluorescence Microscope
−
</td><td>〃
+
</td><td>"submitted 2A"
−
</td><td>する2B
+
 
−
</td><td>未完
+
</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665019"><font color="#007081"><b>BBa_K2665019
−
</td><td>なし
+
</b></font></a></td><td>SdrG
−
</td></tr><tr><td>BBa_K2665022
+
</td><td>－
−
</td><td>Stalk
+
 
</td><td>Tong
 
</td><td>Tong
−
</td><td><a href=""></a>
+
</td><td>Fluorescence Microscope
−
</td><td>〃
+
</td><td>"submitted 2B"
−
</td><td>しない
+
 
−
</td><td>未完
+
</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665022"><font color="#007081"><b>BBa_K2665022
−
</td><td>なし
+
</b></font></a></td><td>Stalk
−
</td></tr><tr><td>BBa_K2665023
+
</td><td>－
−
</td><td>ybbr-tag
+
 
</td><td>Tong
 
</td><td>Tong
−
</td><td><a href=""></a>
+
</td><td>Fluorescence Microscope
−
</td><td>K2665018と2665019を参照しろと書く
+
</td><td>not submitted
−
</td><td>しない
+
 
−
</td><td>未完
+
</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665023"><font color="#007081"><b>BBa_K2665023
−
</td><td>なし
+
</b></font></a></td><td>ybbr-tag
−
</td></tr><tr><td>BBa_K2665024
+
</td><td>Refer to the following<br>
−
</td><td>ddFLN4
+
<a href="http://parts.igem.org/Part:BBa_K2665018"><font color="#007081">K2665018</font></a>, <a href="http://parts.igem.org/Part:BBa_K2665019"><font color="#007081">K2665019
 +
</font></a>
 
</td><td>Tong
 
</td><td>Tong
−
</td><td><a href=""></a>
+
</td><td>－
−
</td><td>K2665018と2665020を参照しろと書く
+
</td><td>not submitted
−
</td><td>しない
+
−
</td><td>未完
+
−
</td><td>なし
+
−
</td></tr>
+
  
 +
</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665024"><font color="#007081"><b>BBa_K2665024
 +
</b></font></a></td><td>ddFLN4
 +
</td><td>Refer to the following<br>
 +
<a href="http://parts.igem.org/Part:BBa_K2665018"><font color="#007081">K2665018</font></a>, <a href="http://parts.igem.org/Part:BBa_K2665020"><font color="#007081">K2665020
 +
</font></a>
 +
</td><td>Tong
 +
</td><td>－
 +
</td><td>not submitted
 +
 +
 +
</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665025"><font color="#007081"><b>BBa_K2665025
 +
</b></font></a></td><td>SED1 secretion signal
 +
</td><td>－
 +
</td><td>Tong
 +
</td><td>－
 +
</td><td>not submitted
 +
 +
</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665026"><font color="#007081"><b>BBa_K2665026
 +
</b></font></a></td><td>SED1 anchoring domain
 +
</td><td>－
 +
</td><td>Tong
 +
</td><td>－
 +
</td><td>not submitted
 +
</td></tr>
  
  
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<!-- Table end -->
 
<!-- Table end -->
  
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<h5 id="methods"><font face="Segoe UI">4) Methods</font></h5>
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<br>
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<!------------------------------------------------------BLOCK Methods PY---------------------------------------------------------------->
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<br>
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<!-- ##############The below part of block is Generated by Methods.py Author: OHAD################# -->
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<br>
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<h6 id = "Miniprep"><font face="Segoe UI">4-1 Miniprep</font></h6>
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<br>
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<p>
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<br>
−
Minipreps were performed using FastGene™Plasmid Mini Kit according to the manufacturer's protocols.
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<br>
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</p>
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−
<!-- ~~~~~~~~~~~~~Part END~~~~~~~~~~~~~~ -->
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<br>
 
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Latest revision as of 00:52, 18 October 2018

Basic Parts
Number parts Remark Designer Remark2 Subimittion
BBa_K2665000 TDH3 promoter － Tong － not submitted
BBa_K2665001 CYCterm fused with 6xHis tag and stop codon － Tong － not submitted
BBa_K2665002 Mangrin ORF － Tong － not submitted
BBa_K2665003 ZrGPD1 ORF － Tong － not submitted
BBa_K2665005 SseNHX1 ORF － Tong － not submitted
BBa_K2665006 AVP1 ORF including the stop codon Tong － not submitted
BBa_K2665007 AtHKT1 ORF － Tong － not submitted
BBa_K2665008 ZrFPS1 ORF － Tong － not submitted
BBa_K2665018 Fgb － Tong Fluorescence Microscope "submitted 2A"
BBa_K2665019 SdrG － Tong Fluorescence Microscope "submitted 2B"
BBa_K2665022 Stalk － Tong Fluorescence Microscope not submitted
BBa_K2665023 ybbr-tag Refer to the following
K2665018, K2665019 		Tong － not submitted
BBa_K2665024 ddFLN4 Refer to the following
K2665018, K2665020 		Tong － not submitted
BBa_K2665025 SED1 secretion signal － Tong － not submitted
BBa_K2665026 SED1 anchoring domain － Tong － not submitted