Difference between revisions of "Team:Kyoto/Composite Parts"

 
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    <h1>Composite part(未)</h1><br><p>締め切り：　、原稿担当：</p>
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<img src="https://static.igem.org/mediawiki/2018/1/11/T--Kyoto--upbotton.jpg"></a></div>
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<div id="BACKGROUND">
  
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  <p>In this section, we nominate our Best Composite Part: T7 promoter-RRE ( <a href="http://parts.igem.org/Part:BBa_K2403002"> BBa_K2403002</a>). Expression of RNA with an RRE (Rev Response Element), provides a way to achieve nuclear export of any RNA, especially those with complex structure. </p>
 
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  <h5>Best Composite Part</h5>
 
  
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  <P>Construction
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<div style='padding-top: 100px;'><h1 id="wrapper"><img src="https://static.igem.org/mediawiki/2018/7/78/T--Kyoto--compositeparts.png" width="30%"></div></h1>
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We combined our RRE part (<a href="http://parts.igem.org/Part:BBa_K2403001">BBa_K2403001</a>) along with a T7 promoter (<a href="http://parts.igem.org/Part:BBa_K1614000">BBa_K1614000</a>) for RNA expression in the high copy BioBrick assembly plasmid pSB1C3.</p>
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<br>       
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<h5>Composite Parts</h5>
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<table>
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<tr><th>Number
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</th><th width=30%>parts
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</th><th>Remark
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</th><th width=8%>Designer
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</th><th>Experimental　data
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</th><th>Subimittion
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</th></tr>
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<tr><td><a href="http://parts.igem.org/Part:BBa_K2665011"><font color="#007081"><b>BBa_K2665011
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</b></font></a></td><td>TDH3-Mangrin-6xHis-CYC
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</td><td>－
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</td><td>Tong
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</td><td>Spot Assay<br> and Boil method
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</td><td>"submitted １A"
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</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665012"><font color="#007081"><b>BBa_K2665012
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</b></font></a></td><td>TDH3-ZrGPD1-6xHis-CYC
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</td><td>－
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</td><td>Tong
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</td><td>〃
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</td><td>"submitted １B"
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</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665013"><font color="#007081"><b>BBa_K2665013
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</b></font></a></td><td>TDH3-AtNHXS1-6xHis-CYC
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</td><td>－
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</td><td>Tong
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</td><td>〃
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</td><td>"submitted １C"
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</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665014"><font color="#007081"><b>BBa_K2665014
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</b></font></a></td><td>TDH3-SseNHX1-6xHis-CYC
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</td><td>codon optimized
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</td><td>Tong
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</td><td>〃
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</td><td>"submitted １E"
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</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665015"><font color="#007081"><b>BBa_K2665015
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</b></font></a></td><td>TDH3-AVP1-6xHis-CYC
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</td><td>not excluded the stop codon
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</td><td>Tong
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</td><td>〃
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</td><td>"submitted １D"
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</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665016"><font color="#007081"><b>BBa_K2665016
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</b></font></a></td><td>TDH3-AtHKT1-6xHis-CYC
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</td><td>－
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</td><td>Tong
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</td><td>〃
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</td><td>"submitted１F"
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</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665017"><font color="#007081"><b>BBa_K2665017
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</b></font></a></td><td>TDH3-ZrFPS1-6xHis-CYC
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</td><td>－
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</td><td>Tong
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</td><td>〃
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</td><td>not submitted
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</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665020"><font color="#007081"><b>BBa_K2665020
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</b></font></a></td><td>Fgb-stalk
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</td><td>－
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</td><td>Tong
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</td><td>〃
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</td><td>"submitted 2C"
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</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665021"><font color="#007081"><b>BBa_K2665021
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</b></font></a></td><td>SdrG-stalk
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</td><td>－
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</td><td>Tong
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</td><td>〃
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</td><td>not submitted
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</td></tr>
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  <p> iGEMers can simply digest our composite part <a href="http://parts.igem.org/Part:BBa_K2403002">BBa_K2403002</a> with the restriction enzymes <i>XbaI</i> and <i>SpeI</i>, and ligate their custom sequence which will be expressed as an RNA that includes an RRE. Along with expression of Rev protein, they can easily export their RNA from the nucleus.</p>
 
  
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  <p>This part makes it possible for complex RNA to be transported to the cytoplasm by CRM1.<a href="https://2017.igem.org/Team:Kyoto/Results">Please see our result for more detailed information.</a>
 
 
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Latest revision as of 00:53, 18 October 2018


Composite Parts
Number parts Remark Designer Experimental　data Subimittion
BBa_K2665011 TDH3-Mangrin-6xHis-CYC － Tong Spot Assay
and Boil method 		"submitted １A"
BBa_K2665012 TDH3-ZrGPD1-6xHis-CYC － Tong 〃 "submitted １B"
BBa_K2665013 TDH3-AtNHXS1-6xHis-CYC － Tong 〃 "submitted １C"
BBa_K2665014 TDH3-SseNHX1-6xHis-CYC codon optimized Tong 〃 "submitted １E"
BBa_K2665015 TDH3-AVP1-6xHis-CYC not excluded the stop codon Tong 〃 "submitted １D"
BBa_K2665016 TDH3-AtHKT1-6xHis-CYC － Tong 〃 "submitted１F"
BBa_K2665017 TDH3-ZrFPS1-6xHis-CYC － Tong 〃 not submitted
BBa_K2665020 Fgb-stalk － Tong 〃 "submitted 2C"
BBa_K2665021 SdrG-stalk － Tong 〃 not submitted