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| − | <h2 id="title" class="retroshadow">Attributions</h2>
| + | $("#title").text("Safety") |
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| − | | + | <div class="body"> |
| − | | + | <p><span style="font-weight: 400;"> |
| − | | + | Laboratory equipment can be dangerous if users are unaware of the potential safety hazard. Thus, we strive to make sure our team members have a solid understanding before we operate. |
| − | <div class="column full_size"> | + | |
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| − | <p>This page is your opportunity to explain what parts of your project you did and what was done by technicians, advisers, etc. This requirement is not about literature references - these can and should be displayed throughout your wiki. | + | |
| | </p> | | </p> |
| − | | + | <h1><strong> |
| − | <h3> Bronze Medal Criterion #3</h3> | + | Safety Considerations of our Project Design |
| − | <p> All of the work done in your project must be attributed correctly on this page. You must clearly state the work that was done by the students on your team and note any work that was done by people outside of your team, including the host labs, advisors, instructors, and individuals not on the team roster.
| + | </strong></h1> |
| − | <br><br> | + | <p><span style="font-weight: 400;"> |
| − | Please see the <a href="https://2018.igem.org/Judging/Medals">Medals requirements page</a> for more details.</p>
| + | <u><strong>Organisms:</strong></u> |
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| − | <h3> What should this page contain?</h3> | + | |
| − | | + | |
| | <ul> | | <ul> |
| − | <li>Clearly state what the team accomplished</li> | + | <li> |
| − | <li>General Support</li> | + | We did not use any living horseshoe crabs for our project as our work has been completed using solely bacteria: <i>E. Coli</i> and <i>Bacillus subtilis 3NA</i>. |
| − | <p1>Thank you to our advisers Alan Bohn, Yoshitaka Goto, Zach Mueller, Anastasia Nicolov for giving us tips, feedback, and guidance on wetlab, outreach, and more! </p1>
| + | </li> |
| − | <li>Project support and advice</li>
| + | <li> |
| − | <p1>Jay Yung and the Washington iGEM for letting us use their plate readers for the InterLab study!</p1>
| + | Some strains of <i>E. Coli</i> can sometimes cause food poisoning. Considering the potential detrimental health effect, we used the <i>E. Coli </i>strain <i>DH5-alpha</i>, which is unharmful for humans. |
| − | <li>Fundraising help and advice</li>
| + | </li> |
| − | <li>Lab support</li> | + | <li> |
| − | <p1>Dr. Dan Ziegler from Ohio State University for kindly giving us Bacillus Strains and workable integration vectors.</p1> | + | The other strain of bacteria that we frequently work with is <i>Bacillus Subtilis 3NA</i>, which is also not considered pathogenic to humans. |
| − | <li>Difficult technique support</li> | + | </li> |
| − | <p1>Judy Nguyen from Tacoma RAINmakers! Angel Wong from Washington iGEM!</p1> | + | <li> |
| − | <li>Project advisor support</li>
| + | All of our used plates are disposed in appropriate bio-hazard containers, and our cell cultures are bleached to avoid the spread of laboratory anti-biotics and bacteria. |
| − | <li>Wiki support</li> | + | </li> |
| − | <p1>William Kwok from the Washington iGEM team!</p1> | + | |
| − | <li>Presentation coaching</li> | + | |
| − | <p1>Angel Wong and Andrew Hu from the Washington Team. And everyone who attended the PNW iGEM meetup to critique us!</p1>
| + | |
| − | <li>Human Practices support</li>
| + | |
| − | <li> Thanks and acknowledgements for all other people involved in helping make a successful iGEM team</li>
| + | |
| − | <p1>Dr. Thomas Novitsky!</p1>
| + | |
| | </ul> | | </ul> |
| − | </div> | + | </p> |
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| − | <div class="column third_size"> | + | <p><span style="font-weight: 400;"> |
| − | <p>Tell us if your institution teaches an iGEM or synthetic biology class and when you started your project:</p> | + | <u><strong>Lab Safety:</strong></u> |
| − | <ul>
| + | <br> |
| − | <li>Does your institution teach an iGEM or synthetic biology course?</li>
| + | Although we do not wear lab coats and goggles for most of the time, we have strict rules on attire. We wear closed-toed shoes, wear gloves, and wash our hands thoroughly before we leave the lab. In addition, we never eat/drink on the bench, and ensure that we clean up our lab space after each day. |
| − | <li>When did you start this course?</li> | + | </p> |
| − | <li>Are the syllabus and course materials freely available online?</li>
| + | |
| − | <li>When did you start your brainstorming?</li>
| + | |
| − | <li>When did you start in the lab?</li>
| + | |
| − | <li>When did you start working on your project?</li>
| + | |
| − | </ul> | + | |
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| − | <h3>Inspiration</h3>
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| − | <p>Take a look at what other teams have done:</p>
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| − | <ul>
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| − | <li><a href="https://2011.igem.org/Team:Imperial_College_London/Team">2011 Imperial College London</a> (scroll to the bottom)</li>
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| − | <li><a href="https://2014.igem.org/Team:Exeter/Attributions">2014 Exeter </a></li>
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| − | <li><a href="https://2014.igem.org/Team:Melbourne/Attributions">2014 Melbourne </a></li>
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| − | <li><a href="https://2014.igem.org/Team:Valencia_Biocampus/Attributions">2014 Valencia Biocampus</a></li>
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| − | </ul>
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| − | <h3> Why is this page needed? </h3>
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| − | <p>The Attribution requirement helps the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, but you must report what work your team did and what work was done by others.</p>
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| − | <p>
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| − | For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.</p>
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| − | <h3> Can we base our project on a previous one? </h3>
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| − | <p>Yes! You can have a project based on a previous team, or based on someone else's idea, <b>as long as you state this fact very clearly and give credit for the original project.</b> </p>
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| − | <p1>Our project can be considered a continuation of the GSU iGEM project with LAL and Human chorionic gonadotropin (hCG) beta subunit.</p1>
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Laboratory equipment can be dangerous if users are unaware of the potential safety hazard. Thus, we strive to make sure our team members have a solid understanding before we operate.
Safety Considerations of our Project Design
Organisms:
-
We did not use any living horseshoe crabs for our project as our work has been completed using solely bacteria: E. Coli and Bacillus subtilis 3NA.
-
Some strains of E. Coli can sometimes cause food poisoning. Considering the potential detrimental health effect, we used the E. Coli strain DH5-alpha, which is unharmful for humans.
-
The other strain of bacteria that we frequently work with is Bacillus Subtilis 3NA, which is also not considered pathogenic to humans.
-
All of our used plates are disposed in appropriate bio-hazard containers, and our cell cultures are bleached to avoid the spread of laboratory anti-biotics and bacteria.
Lab Safety:
Although we do not wear lab coats and goggles for most of the time, we have strict rules on attire. We wear closed-toed shoes, wear gloves, and wash our hands thoroughly before we leave the lab. In addition, we never eat/drink on the bench, and ensure that we clean up our lab space after each day.
