Difference between revisions of "Team:BOKU-Vienna"

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{{BOKU-Vienna}}
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<h1> Welcome to iGEM 2018! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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                    <a href="https://2018.igem.org/Team:BOKU-Vienna">Home <span class="sr-only">(current)</span></a>
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                    <img src="https://static.igem.org/mediawiki/2018/b/b5/T--BOKU-Vienna--2018_Home1.jpg">
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                    <a href="https://2018.igem.org/Team:BOKU-Vienna/Team">Team</a>
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                    <a href="https://2018.igem.org/Team:BOKU-Vienna/Description">Project</a>
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<h3>Before you start</h3>
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<p> Please read the following pages:</p>
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                    <a href="https://2018.igem.org/Team:BOKU-Vienna/Parts">Parts</a>
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<li> <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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                    <img src="https://static.igem.org/mediawiki/2018/2/2e/T--BOKU-Vienna--2018_Parts3.jpg">
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<li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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                    <a href="https://2018.igem.org/Team:BOKU-Vienna/Safety">Safety</a>
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                    <a href="https://2018.igem.org/Team:BOKU-Vienna/Human_Practices">Human Practices</a>
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                    <a href="https://2018.igem.org/Team:BOKU-Vienna/Applied_Design">Awards</a>
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<h3> Styling your wiki </h3>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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                    <a href="https://igem.org/2018_Judging_Form?team=BOKU-Vienna">Judging Form</a>
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            <h1>Team Vienna 2018</h1>
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            <h2>We are presenting Robocrop</h2>
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<p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
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                <a class="h1 h1-green" href="https://2018.igem.org/Team:BOKU-Vienna#collapseWhy" data-toggle="collapse" data-target="#collapseWhy" aria-expanded="false" aria-controls="collapseWhy">
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                    Why
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<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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UPLOAD FILES
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                    <p>We want to establish a way of communicating with eukaryotes and creating a human-organism interaction. We would like to tell an organism when it should perform a certain task, be it the production of a pesticide or the induction of the gene which induces flowering. What all these applications have in common is that we are not forced to change the organism’s environment to get the desired reaction, but instead we tell it how and when to do a certain task. Such a communication platform can create countless new applications in agriculture, research and many other fields.</p>
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<h3> Wiki template information </h3>
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                How
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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                <p>The goal of our project is to design a genetic mechanism which enables us to switch a gene of interest ON or OFF with a single dose of a signal molecule, similar to switching a lightbulb ON or OFF.</p>
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                <p>This enables us to define which genes should be expressed at what point in time. Our toggle switch is designed in a way, that any gene of interest can be cloned into the system and toggled with the corresponding signal molecules. There are countless possibilities for genes that could be cloned into such a system, starting from genes which induce the flowering of plants or the production of a certain resistance gene. In our example, the production of a short lived species of GFP is induced or repressed as soon as either the ON or OFF signal molecule enters the cell.</p>
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                <a type="button" class="btn btn-secondary" href="https://2018.igem.org/Team:BOKU-Vienna/Design" data-toggle="tooltip" data-placement="top" title="If you want to find out more about how we found our project and why we chose to get into the information processing track, click on this link.">
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<h3> Editing your wiki </h3>
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                    Design
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>  
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<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
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<a href="https://2018.igem.org/wiki/index.php?title=Team:BOKU-Vienna&action=edit">  
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EDIT PAGE
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                What
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                <p>The system is based on a dCas9 toggle switch, which can be activated or shut-down by a single dose of a signal molecule and stays in its state until a different signal is added. The system relies on dCas9 binding in complex with a specific guide-RNA to the respective site in the promoter of a gene and thereby blocking its transcription. This means that the expression of any gene behind this blocked site is being shut down. As signal molecules, we first created receptors for ethanol, estradiol, and copper. Since these receptors are rather complicated to create and represent a critical element in our system we decided to implement another way of inducing the system, which allows us to test the switch without the need of a functioning receptor.  We came up with the idea of using guide-RNA, contained in liposomes to activate the toggle switch.</p>
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<h3>Tips</h3>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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    </div>        <div class="card-group">
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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            <a class="cardelement" href="https://2018.igem.org/Team:BOKU-Vienna/Lab_Moments">
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
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                <img class="card-img-top" src="https://static.igem.org/mediawiki/2018/3/34/T--BOKU-Vienna--2018_grouppic.jpg" alt="Group Picture">
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<li>Have lots of fun! </li>
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                <div class="card-body">
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                    <h5 class="card-title">Our best Lab moments</h5>
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</div>
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                    <p class="card-text">Take a look at our lab work and other activities during our work at these project!</p>
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            </a>
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<h3>Inspiration</h3>
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                <a class="cardelement" href="https://2018.igem.org/Team:BOKU-Vienna/Best_Videos">
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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                <img class="card-img-top" src="https://static.igem.org/mediawiki/2018/4/45/T--BOKU-Vienna--2018_bestfilm_landing.jpg" alt="Bestiflm Landing">
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<ul>
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                <div class="card-body">
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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                    <h5 class="card-title">Our best videos</h5>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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                    <p class="card-text">Take a look at our video wall.</p>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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                </div>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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                </a>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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            </div>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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            <div class="card col-md-3 fly-in">
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</ul>
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                <a class="cardelement" href="https://2018.igem.org/Team:BOKU-Vienna/Newsletter">
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</div>
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                    <img class="card-img-top" src="https://static.igem.org/mediawiki/2018/f/fd/T--BOKU-Vienna--2018_newsletter-landing.png" alt="Newsletter Landing Picture">
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                    <div class="card-body">
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                        <h5 class="card-title">Our Newsletter Database</h5>
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                        <p class="card-text">Find our newsletter that we send to our sponsors during the work to inform about updates to our project.</p>
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                    </div>
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                </a>
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 +
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Latest revision as of 00:53, 18 October 2018


Team Vienna 2018

We are presenting Robocrop

We want to establish a way of communicating with eukaryotes and creating a human-organism interaction. We would like to tell an organism when it should perform a certain task, be it the production of a pesticide or the induction of the gene which induces flowering. What all these applications have in common is that we are not forced to change the organism’s environment to get the desired reaction, but instead we tell it how and when to do a certain task. Such a communication platform can create countless new applications in agriculture, research and many other fields.

The goal of our project is to design a genetic mechanism which enables us to switch a gene of interest ON or OFF with a single dose of a signal molecule, similar to switching a lightbulb ON or OFF.

This enables us to define which genes should be expressed at what point in time. Our toggle switch is designed in a way, that any gene of interest can be cloned into the system and toggled with the corresponding signal molecules. There are countless possibilities for genes that could be cloned into such a system, starting from genes which induce the flowering of plants or the production of a certain resistance gene. In our example, the production of a short lived species of GFP is induced or repressed as soon as either the ON or OFF signal molecule enters the cell.

Design

The system is based on a dCas9 toggle switch, which can be activated or shut-down by a single dose of a signal molecule and stays in its state until a different signal is added. The system relies on dCas9 binding in complex with a specific guide-RNA to the respective site in the promoter of a gene and thereby blocking its transcription. This means that the expression of any gene behind this blocked site is being shut down. As signal molecules, we first created receptors for ethanol, estradiol, and copper. Since these receptors are rather complicated to create and represent a critical element in our system we decided to implement another way of inducing the system, which allows us to test the switch without the need of a functioning receptor. We came up with the idea of using guide-RNA, contained in liposomes to activate the toggle switch.

Description
Group Picture
Our best Lab moments

Take a look at our lab work and other activities during our work at these project!
Bestiflm Landing
Our best videos

Take a look at our video wall.
Newsletter Landing Picture
Our Newsletter Database

Find our newsletter that we send to our sponsors during the work to inform about updates to our project.