Difference between revisions of "Team:Kyoto/Parts Collection"

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<div style='padding-top: 100px;'><h1 id="wrapper"><img src="https://static.igem.org/mediawiki/2018/1/1d/T--Kyoto--basicparts.png" width="30%"></div></h1>
+
<div style='padding-top: 100px;'><h1 id="wrapper"><img src="https://static.igem.org/mediawiki/2018/a/a8/T--Kyoto--partscollection.png" width="30%"></div></h1>
  
 +
<div class="box27">
 +
    <span class="box-title"><font face="Segoe UI">The function of our Part Collection</font></span>
 +
          <ul class="index1">
 +
            <li>This Part Collection enables to create a biological desalination yeast with aggregation system.
 +
BBa_K2665018, BBa_K2665019 and BBa_K2665020 work for yeast aggregation. BBa_K2665011 and BBa_K2665012 can be used for raising yeasts' halotorelance, and parts of BBa_K2665013 to BBa_K2665016 enhance yeasts' ability of Na+ uptake. BBa_K2665011 to BBa_K2665016 include a constitutive yeast promoter and a common terminator so iGEMers can use them without assembly to express these proteins in yeast.</ul>
 +
          </div>
  
 
<!-- Table Generated by TableMaker.py Author: OHAD -->
 
<!-- Table Generated by TableMaker.py Author: OHAD -->
−
<h5 id="Basic_Parts">Basic Parts</h5>
+
<h5 id="Basic_Parts">Parts collection</h5>
  
  
Line 136: Line 145:
 
</th></tr>
 
</th></tr>
  
−
<tr><td><a href="http://parts.igem.org/Part:BBa_K2665000"><font color="#007081"><b>BBa_K2665000
+
<tr><td><a href="http://parts.igem.org/Part:BBa_K2665011"><font color="#007081"><b>BBa_K2665011
−
</b></font></a></td><td>TDH3 promoter
+
</b></font></a></td><td>TDH3-Mangrin-6xHis-CYC
 
</td><td>－
 
</td><td>－
 
</td><td>Tong
 
</td><td>Tong
−
</td><td>－
+
</td><td>Spot Assay<br> and Boil method
−
</td><td>not submitted
+
</td><td>"submitted １A"
  
−
</td></tr><tr><td><a href=" http://parts.igem.org/Part:BBa_K2665001"><font color="#007081"><b>BBa_K2665001
+
</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665012"><font color="#007081"><b>BBa_K2665012
−
</b></font></a></td><td>CYCterm fused with 6xHis tag and stop codon
+
</b></font></a></td><td>TDH3-ZrGPD1-6xHis-CYC
 
</td><td>－
 
</td><td>－
 
</td><td>Tong
 
</td><td>Tong
−
</td><td>－
+
</td><td>〃
−
</td><td>not submitted
+
</td><td>"submitted １B"
  
−
</td></tr><tr><td><a href=" http://parts.igem.org/Part:BBa_K2665002"><font color="#007081"><b>BBa_K2665002
+
</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665013"><font color="#007081"><b>BBa_K2665013
−
</b></font></a></td><td>Mangrin ORF
+
</b></font></a></td><td>TDH3-AtNHXS1-6xHis-CYC
 
</td><td>－
 
</td><td>－
 
</td><td>Tong
 
</td><td>Tong
−
</td><td>－
+
</td><td>〃
−
</td><td>not submitted
+
</td><td>"submitted １C"
  
−
</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665003"><font color="#007081"><b>BBa_K2665003
+
</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665014"><font color="#007081"><b>BBa_K2665014
−
</b></font></a></td><td>ZrGPD1 ORF
+
</b></font></a></td><td>TDH3-SseNHX1-6xHis-CYC
−
</td><td>－
+
</td><td>codon optimized
 
</td><td>Tong
 
</td><td>Tong
−
</td><td>－
+
</td><td>〃
−
</td><td>not submitted
+
</td><td>"submitted １E"
  
−
</td></tr><tr><td><a href=""><font color="#007081"><b>BBa_K2665004
+
</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665015"><font color="#007081"><b>BBa_K2665015
−
</b></font></a></td><td>AtNHXS1 ORF
+
</b></font></a></td><td>TDH3-AVP1-6xHis-CYC
−
</td><td>－
+
</td><td>not excluded the stop codon
 
</td><td>Tong
 
</td><td>Tong
−
</td><td>－
+
</td><td>〃
−
</td><td>not submitted
+
</td><td>"submitted １D"
  
−
</td></tr><tr><td><a href=""><font color="#007081"><b>BBa_K2665005
+
</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665016"><font color="#007081"><b>BBa_K2665016
−
</b></font></a></td><td>SseNHX1 ORF
+
</b></font></a></td><td>TDH3-AtHKT1-6xHis-CYC
 
</td><td>－
 
</td><td>－
 
</td><td>Tong
 
</td><td>Tong
−
</td><td>－
+
</td><td>〃
−
</td><td>not submitted
+
</td><td>"submitted１F"
−
 
+
−
</td></tr><tr><td><a href=""><font color="#007081"><b>BBa_K2665006
+
−
</b></font></a></td><td>AVP1 ORF
+
−
</td><td>including the stop codon
+
−
</td><td>Tong
+
−
</td><td>－
+
−
</td><td>not submitted
+
−
 
+
−
</td></tr><tr><td><a href=""><font color="#007081"><b>BBa_K2665007
+
−
</b></font></a></td><td>AtHKT1 ORF
+
−
</td><td>－
+
−
</td><td>Tong
+
−
</td><td>－
+
−
</td><td>not submitted
+
−
 
+
−
</td></tr><tr><td><a href=""><font color="#007081"><b>BBa_K2665008
+
−
</b></font></a></td><td>ZrFPS1 ORF
+
−
</td><td>－
+
−
</td><td>Tong
+
−
</td><td>－
+
−
</td><td>not submitted
+
−
 
+
  
 
</td></tr><tr><td><a href=" http://parts.igem.org/Part:BBa_K2665018"><font color="#007081"><b>BBa_K2665018
 
</td></tr><tr><td><a href=" http://parts.igem.org/Part:BBa_K2665018"><font color="#007081"><b>BBa_K2665018
Line 207: Line 194:
 
</td><td>"submitted 2A"
 
</td><td>"submitted 2A"
  
−
</td></tr><tr><td><a href=""><font color="#007081"><b>BBa_K2665019
+
</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665019"><font color="#007081"><b>BBa_K2665019
 
</b></font></a></td><td>SdrG
 
</b></font></a></td><td>SdrG
 
</td><td>－
 
</td><td>－
Line 214: Line 201:
 
</td><td>"submitted 2B"
 
</td><td>"submitted 2B"
  
−
</td></tr><tr><td><a href=""><font color="#007081"><b>BBa_K2665022
+
</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665020"><font color="#007081"><b>BBa_K2665020
−
</b></font></a></td><td>Stalk
+
</b></font></a></td><td>Fgb-stalk
 
</td><td>－
 
</td><td>－
 
</td><td>Tong
 
</td><td>Tong
−
</td><td>Fluorescence Microscope
+
</td><td>〃
−
</td><td>not submitted
+
</td><td>"submitted 2C"
−
 
+
−
</td></tr><tr><td><a href=""><font color="#007081"><b>BBa_K2665023
+
−
</b></font></a></td><td>ybbr-tag
+
−
</td><td>Refer to the following<br>
+
−
<a href="http://parts.igem.org/Part:BBa_K2665018"><font color="#007081">K2665018</font></a>, <a href="http://parts.igem.org/Part:BBa_K2665019"><font color="#007081">K2665019
+
−
</font></a>
+
−
</td><td>Tong
+
−
</td><td>－
+
−
</td><td>not submitted
+
−
 
+
−
</td></tr><tr><td><a href=""><font color="#007081"><b>BBa_K2665024
+
−
</b></font></a></td><td>ddFLN4
+
−
</td><td>Refer to the following<br>
+
−
<a href="http://parts.igem.org/Part:BBa_K2665018"><font color="#007081">K2665018</font></a>, <a href="http://parts.igem.org/Part:BBa_K2665020"><font color="#007081">K2665020
+
−
</font></a>
+
−
</td><td>Tong
+
−
</td><td>－
+
−
</td><td>not submitted
+
−
 
+
−
 
+
−
</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665025"><font color="#007081"><b>BBa_K2665025
+
−
</b></font></a></td><td>SED1 secretion signal
+
−
</td><td>－
+
−
</td><td>Tong
+
−
</td><td>－
+
−
</td><td>not submitted
+
−
 
+
−
</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665026"><font color="#007081"><b>BBa_K2665026
+
−
</b></font></a></td><td>SED1 anchoring domain
+
−
</td><td>－
+
−
</td><td>Tong
+
−
</td><td>－
+
−
</td><td>not submitted
+
 
</td></tr>
 
</td></tr>
  
 +
</table>
  
−
 
−
</table>
 
 
<!-- Table end -->
 
<!-- Table end -->
  

Latest revision as of 00:54, 18 October 2018

The function of our Part Collection
  • This Part Collection enables to create a biological desalination yeast with aggregation system. BBa_K2665018, BBa_K2665019 and BBa_K2665020 work for yeast aggregation. BBa_K2665011 and BBa_K2665012 can be used for raising yeasts' halotorelance, and parts of BBa_K2665013 to BBa_K2665016 enhance yeasts' ability of Na+ uptake. BBa_K2665011 to BBa_K2665016 include a constitutive yeast promoter and a common terminator so iGEMers can use them without assembly to express these proteins in yeast.
Parts collection
Number parts Remark Designer Remark2 Subimittion
BBa_K2665011 TDH3-Mangrin-6xHis-CYC － Tong Spot Assay
and Boil method 		"submitted １A"
BBa_K2665012 TDH3-ZrGPD1-6xHis-CYC － Tong 〃 "submitted １B"
BBa_K2665013 TDH3-AtNHXS1-6xHis-CYC － Tong 〃 "submitted １C"
BBa_K2665014 TDH3-SseNHX1-6xHis-CYC codon optimized Tong 〃 "submitted １E"
BBa_K2665015 TDH3-AVP1-6xHis-CYC not excluded the stop codon Tong 〃 "submitted １D"
BBa_K2665016 TDH3-AtHKT1-6xHis-CYC － Tong 〃 "submitted１F"
BBa_K2665018 Fgb － Tong Fluorescence Microscope "submitted 2A"
BBa_K2665019 SdrG － Tong Fluorescence Microscope "submitted 2B"
BBa_K2665020 Fgb-stalk － Tong 〃 "submitted 2C"