Difference between revisions of "Team:iTesla-SoundBio/Safety"

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<h1> Safety </h1>
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<p>Please visit the <a href="https://2018.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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Laboratory equipment can be dangerous if users are unaware of the potential safety hazard. Thus, we strive to make sure our team members have a solid understanding before we operate.
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Safety Considerations of our Project Design
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<h3>Safe Project Design</h3>
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<u><strong>Organisms:</strong></u>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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We did not use any living horseshoe crabs for our project as our work has been completed using solely bacteria: <i>E. Coli</i> and <i>Bacillus subtilis 3NA</i>.
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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Some strains of <i>E. Coli</i> can sometimes cause food poisoning. Considering the potential detrimental health effect, we used the <i>E. Coli </i>strain <i>DH5-alpha</i>, which is unharmful for humans.
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The other strain of bacteria that we frequently work with is <i>Bacillus Subtilis 3NA</i>, which is also not considered pathogenic to humans. 
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All of our used plates are disposed in appropriate bio-hazard containers, and our cell cultures are bleached to avoid the spread of laboratory anti-biotics and bacteria.
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<u><strong>Lab Safety:</strong></u>
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Although we do not wear lab coats and goggles for most of the time, we have strict rules on attire. We wear closed-toed shoes, wear gloves, and wash our hands thoroughly before we leave the lab. In addition, we never eat/drink on the bench, and ensure that we clean up our lab space after each day.
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<h3>Safe Lab Work</h3>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<h3>Safe Shipment</h3>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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Latest revision as of 00:55, 18 October 2018

Safety Protocols
InterLab
Team Members Attributions
Human Practices Collaborations
Project Description Design Results Biobrick Parts Lab Notebook
iT-SB 2018 iT-SB 2017

Laboratory equipment can be dangerous if users are unaware of the potential safety hazard. Thus, we strive to make sure our team members have a solid understanding before we operate.

Safety Considerations of our Project Design

Organisms:

  • We did not use any living horseshoe crabs for our project as our work has been completed using solely bacteria: E. Coli and Bacillus subtilis 3NA.
  • Some strains of E. Coli can sometimes cause food poisoning. Considering the potential detrimental health effect, we used the E. Coli strain DH5-alpha, which is unharmful for humans.
  • The other strain of bacteria that we frequently work with is Bacillus Subtilis 3NA, which is also not considered pathogenic to humans.
  • All of our used plates are disposed in appropriate bio-hazard containers, and our cell cultures are bleached to avoid the spread of laboratory anti-biotics and bacteria.

Lab Safety:
Although we do not wear lab coats and goggles for most of the time, we have strict rules on attire. We wear closed-toed shoes, wear gloves, and wash our hands thoroughly before we leave the lab. In addition, we never eat/drink on the bench, and ensure that we clean up our lab space after each day.