Difference between revisions of "Team:H14Z1 Hangzhou/Design"

 
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                     <!------------------------Liver function and protection------------------------------ -->
 
                     <!------------------------Liver function and protection------------------------------ -->
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                     <h3 class="content_subtitle">Liver function and protection</h3>
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                     <h6 style=" margin-top: 15px;
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                    <p class="content_context"  style="text-indent:2em; text-align:justify">
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                        <a href="http://www.discoverymedicine.com/category/species-and-cell-types/bacterium/lactococcus-lactis/">Lactococcus lactis</a>
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                        is one of the most common organisms used in bacterial drug delivery system.
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                    font-family: 'Open Sans', sans-serif;">Design
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                        <a href="http://www.discoverymedicine.com/category/species-and-cell-types/bacterium/lactococcus-lactis/">Lactococcus lactis</a>
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                         and integration of three different modules (gshF, met K and
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                        is a food-grade bacterium that is widely used in the dairy industry. It is a
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                         cwaA)</h6>
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                        Gram-positive bacterium and therefore lacks endotoxic lipopolysaccharides (LPS) which are
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                    <p><img style="width: 70%; margin-top: 3em" src="https://static.igem.org/mediawiki/2018/4/41/T--H14Z1_Hangzhou--project_design_fig1.png"></p>
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                        associated with commonly used
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                        <a href="http://www.discoverymedicine.com/category/species-and-cell-types/bacterium/gram-negative-bacteria/">Gram-negative bacteria</a>
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                         . It is also non-invasive and non-commensal
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                        and thus has less potential to trigger immunotolerance or side effects upon prolonged use. The
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                        bacterium has an established safety profile through its long use in fermented dairy products
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                        and is considered as a GRAS (generally recognized as safe) microorganism. During the last two
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                        decades, significant advances have been made in the field of lactococcal genetics and protein
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                         expression systems. The most commonly used system is the nisin-controlled gene expression
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                        (NICE) system, containing the nisin promoter. Consequently, we have seen the emergence of new
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                        areas for the application of engineered L. lactis for protein expression,
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                        <a href="http://www.discoverymedicine.com/category/research-technology/nucleic-acid/gene-delivery/">gene delivery</a>,
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                        vaccine delivery, and therapeutic
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                        <a href="http://www.discoverymedicine.com/category/research-technology/drug-delivery-research-technology/">drug delivery.</a>
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                    </p>
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                     <h6 style=" margin-top: 15px;
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                        In the present project, since the oral table administration of GSH and SAM have some
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                        disadvantages, such as low stability and short life span, here we tried to develop a novel
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                        in-vivo strategy of produce and deliver them simultaneously by using NICE system. In the
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                    font-family: 'Open Sans', sans-serif;">Functional characterization of pNZ-SGC in <i>L.lactis</i> NZ9000</h6>
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                        experiment, two-functional GSH synthetase gene (gshF) and SAM synthetase gene (metK) were in
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                     <p><img style="width: 70%; margin-top: 3em" src="https://static.igem.org/mediawiki/2018/e/e8/T--H14Z1_Hangzhou--project_design_fig2.png"></p>
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                        tandem inserted into the expression vector (pNZ8148), and the resulted plasmid (pNZ8148-SG) was
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                        employed to construct the target vector pNZ8148-SGC by introducing adhesion factor gene (cwaA).
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                        This target vector was transformed to get recombinant Lactococcus lacti, which was employed to
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                        produce our “smart yogurt”.
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                     </p>
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                    <h3 class="content_subtitle">
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                        The project is outlined with two stages：
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                    </h3>
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                    <!-- ------------------------Stage A---------------------------- -->
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                        <span style="width:120px; font: bold; font-size: 25px">Stage A</span><span>Construction of Lactococcus lactis NZ9000/pNZ-SGC</span>
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                    </p>
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                    <p><img style="width: 50%; margin-top: 1em" src="https://static.igem.org/mediawiki/2018/b/be/T--H14Z1_Hangzhou--description_projectOverview_fig1.png"></p>
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                    <p><img style="width: 50%; margin-top: 1em" src="https://static.igem.org/mediawiki/2018/c/cd/T--H14Z1_Hangzhou--description_projectOverview_fig12.png"></p>
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                     <p><img style="width: 50%; margin-top: 1em" src="https://static.igem.org/mediawiki/2018/8/87/T--H14Z1_Hangzhou--description_projectOverview_fig3.png"></p>
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                     <!-- ------------------------Stage B---------------------------- -->
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                     <h6 style=" margin-top: 15px;
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                     <p class="mt8 content_context">
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                         <span style="width:120px; font: bold; font-size: 25px">Stage B</span><span>Preparation of smart yogurt with our constructed Lactococcus lactis</span>
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                    font-size: 18px;
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                    font-family: 'Open Sans', sans-serif;">Production of “smart yogurt” with our constructed <i>L.lactis</i>
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                        NZ9000/pNZ-GMcA</h6>
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                    <p><img style="width: 70%; margin-top: 3em" src="https://static.igem.org/mediawiki/2018/6/65/T--H14Z1_Hangzhou--project_design_fig3.png"></p>
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                     <p class="content_context" style="text-align:center; font-size:18px">
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                         <a style="text-decoration: none; font-size: 18px" href="https://www.nyas.org/ebriefings/cytokine-therapies/">https://www.nyas.org/ebriefings/cytokine-therapies/</a>
 
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                    <p><img style="width: 50%; margin-top: 1em" src="https://static.igem.org/mediawiki/2018/6/66/T--H14Z1_Hangzhou--description_projectOverview_fig4.png"></p>
 
 
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Latest revision as of 01:02, 18 October 2018

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Design

Design and integration of three different modules (gshF, met K and cwaA)

Functional characterization of pNZ-SGC in L.lactis NZ9000

Production of “smart yogurt” with our constructed L.lactis NZ9000/pNZ-GMcA

https://www.nyas.org/ebriefings/cytokine-therapies/