Difference between revisions of "Team:Munich/cfp gRNA.html"
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| − | Generating | + | Generating New gRNA |
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| − | gRNA | + | gRNA Generation and Urea Page Gel |
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Latest revision as of 01:22, 18 October 2018
Generation of gRNA (the DNA for the Transcription)
2018/05/25| Participants: | Keno Eilers |
| Protocol: | gRNA production | Notes: | Work at RNAse free bench. | Results: | Coding and non-coding part of digestion site 1, 2 and 3 are annealed. |
Generating MS2 RNA and gRNA
2018/05/28| Participants: | Keno Eilers |
| Protocol: | gRNA production, Phenol Chloroform precipitation | Notes: | No notes. | Results: | DNA has been used for MS2production and gRNA for cpf digest. |
Cpf1 Digest -gRNA Production
2018/05/29| Participants: | Keno Eilers |
| Protocol: | Cpf1 digest, Urea gel | Notes: | No notes. | Results: | No gRNA visible. |
Cpf1 Digest
2018/06/06| Participants: | Keno Eilers |
| Protocol: | Cpf1 digest, Agarose gel | Notes: | No notes. | Results: | No bands visible. |
Generating New gRNA
2018/06/14| Participants: | Keno Eilers |
| Protocol: | gRNA production, Phenol Chloroform precipitation | Notes: | Used DNAse I | Results: | DNA has been used for Urea Page gel |
gRNA Generation and Urea Page Gel
2018/06/18| Participants: | Keno Eilers |
| Protocol: | gRNA production, Urea gel | Notes: | No notes. | Results: |
gRNA1 620 ng/µl gRNA2 591 ng/µl |
Cpf1 Digest with gRNA
2018/06/26| Participants: | Keno Eilers |
| Protocol: | Cpf1 digest, Agarose gel | Notes: | No notes. | Results: | No bands, T7 DNA not clean enough. |