Difference between revisions of "Team:HZAU-China/Notebook"
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<h2><b>1.</b> Verification of Poisson distribution</h2> | <h2><b>1.</b> Verification of Poisson distribution</h2> | ||
<p> | <p> | ||
| − | We infect tumor cells with <i>Salmonella</i> carrying the GFP gene for a period of time and | + | We infect tumor cells (HeLa GSDMD KO cell line) with <i>Salmonella</i> carrying the GFP gene for a period of time and |
| − | count, and the result is that the distribution of <i>Salmonella</i> in the tumor cells | + | then count them, and the result is that the distribution of <i>Salmonella</i> in the tumor cells |
| − | is consistent with | + | is consistent with a Poisson distribution. |
</p> | </p> | ||
<br><br> | <br><br> | ||
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<img src="https://static.igem.org/mediawiki/2018/8/86/T--HZAU-China--Notebook-fig1.png" width="100%" alt=""> | <img src="https://static.igem.org/mediawiki/2018/8/86/T--HZAU-China--Notebook-fig1.png" width="100%" alt=""> | ||
</div> | </div> | ||
| − | <p style="width: 100%; text-align: center !important;"><b>Figure 1.</b> Infection of <i>Salmonella</i> | + | <p style="width: 100%; text-align: center !important;"><b>Figure 1.</b> Infection of HeLa GSDMD KO cells by <i>Salmonella</i> and the result of analysis.</p><br> |
| − | + | <p>The amounts of host cells and bacteria were measured in experiments. | |
| − | <p>The | + | |
</p><br> | </p><br> | ||
</div> | </div> | ||
<div id="float02"> | <div id="float02"> | ||
| − | <p>The concentration of bacteria is converted according to MOI and cell density.<br> | + | <p>The concentration of bacteria is converted according to MOI (multiplicity of infection) and cell density.<br> |
| − | We adjusted As to match the curve | + | We adjusted the parameter As to match the curve with the results of the Salmonella infection experiment. When As is |
| − | equal to 0.0003, the curve simulated by the mathematical model matches the experimental | + | equal to 0.0003, the curve simulated by the mathematical model matches the experimental measurements. |
</p> | </p> | ||
<div style="width: 80%; margin: 20px auto"> | <div style="width: 80%; margin: 20px auto"> | ||
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</div> | </div> | ||
<p style="width: 100%; text-align: center !important;"><b>Figure 2.</b> Parameter fitting of the tumor | <p style="width: 100%; text-align: center !important;"><b>Figure 2.</b> Parameter fitting of the tumor | ||
| − | cells infection experiments. | + | cells infection experiments.Red points are experimental results. |
</p><br> | </p><br> | ||
<p> | <p> | ||
| − | The ‘Nsal’ and ‘Tumor’ | + | The parameters ‘Nsal’ and ‘Tumor’ are already known before the experiment. When the parameter ‘As’ is 0.0003, |
| − | the model results fit the experimental results. | + | the model results fit the experimental results. </p> |
| − | + | ||
</div> | </div> | ||
Revision as of 01:33, 18 October 2018
1. Verification of Poisson distribution
We infect tumor cells (HeLa GSDMD KO cell line) with Salmonella carrying the GFP gene for a period of time and then count them, and the result is that the distribution of Salmonella in the tumor cells is consistent with a Poisson distribution.
Figure 1. Infection of HeLa GSDMD KO cells by Salmonella and the result of analysis.
The amounts of host cells and bacteria were measured in experiments.
The concentration of bacteria is converted according to MOI (multiplicity of infection) and cell density.
We adjusted the parameter As to match the curve with the results of the Salmonella infection experiment. When As is
equal to 0.0003, the curve simulated by the mathematical model matches the experimental measurements.
Figure 2. Parameter fitting of the tumor cells infection experiments.Red points are experimental results.
The parameters ‘Nsal’ and ‘Tumor’ are already known before the experiment. When the parameter ‘As’ is 0.0003, the model results fit the experimental results.
