Difference between revisions of "Team:HZAU-China/Notebook"
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| − | + | <div class="h1">Processing of experiment results</div> | |
| − | + | <h2><b>1.</b> Verification of Poisson distribution</h2> | |
| − | + | <p> | |
| − | + | We infect tumor cells (HeLa GSDMD KO cell line) with <i>Salmonella</i> carrying the GFP gene for a period of time and | |
| − | + | then count them, and the result is that the distribution of <i>Salmonella</i> in the tumor cells | |
| − | + | is consistent with a Poisson distribution. | |
| − | + | </p> | |
| − | + | <br><br> | |
| − | + | <div style="width: 80%; margin: 20px auto"> | |
| − | + | <img src="https://static.igem.org/mediawiki/2018/8/86/T--HZAU-China--Notebook-fig1.png" width="100%" alt=""> | |
| − | + | </div> | |
| − | + | <p style="width: 100%; text-align: center !important;"><b>Figure 1.</b> Infection of HeLa GSDMD KO cells by <i>Salmonella</i> and the result of analysis.</p><br> | |
| − | + | <p>The amounts of host cells and bacteria were measured in experiments. | |
| − | + | </p> | |
| − | + | </div> | |
| − | + | <div id="float02"> | |
| − | + | <p>The concentration of bacteria was calculated according to MOI (multiplicity of infection) and cell density.<br><br> | |
| − | + | We adjusted the parameter As to match the curve with the results of the Salmonella infection experiment. When As is | |
| + | equal to 0.0003, the curve simulated by the mathematical model matches the experimental measurements. | ||
| + | </p> | ||
| + | <div style="width: 80%; margin: 20px auto"> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/8/84/T--HZAU-China--Notebook-fig2.png" width="100%" alt=""> | ||
| + | </div> | ||
| + | <p style="width: 100%; text-align: center !important;"><b>Figure 2.</b> Parameter fitting of the tumor | ||
| + | cells infection experiments. Red points are experimental results. | ||
| + | </p><br> | ||
| + | <p> | ||
| + | The parameters ‘Nsal’ and ‘Tumor’ are already known before the experiment. When the parameter ‘As’ is 0.0003, | ||
| + | the model results best fit the experimental results. </p> | ||
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<span class="biaoti">Improve</span> | <span class="biaoti">Improve</span> | ||
| + | <span class="xsjPic"><img src="https://static.igem.org/mediawiki/2018/7/7c/T--HZAU-China--ysj.svg" alt=""></span> | ||
| + | </div> | ||
| + | <a class="daohangyi" href="https://2018.igem.org/Team:HZAU-China/InterLab"> | ||
| + | <span class="biaoti">InterLab</span> | ||
| + | <span class="xsjPic"><img src="https://static.igem.org/mediawiki/2018/7/7c/T--HZAU-China--ysj.svg" alt=""></span> | ||
| + | </a> | ||
| + | <div class="daohangyi" href="#"> | ||
| + | <span class="biaoti">Notebook</span> | ||
<span class="xsjPic"><img src="https://static.igem.org/mediawiki/2018/8/8d/T--HZAU-China--xjt.svg" alt=""></span> | <span class="xsjPic"><img src="https://static.igem.org/mediawiki/2018/8/8d/T--HZAU-China--xjt.svg" alt=""></span> | ||
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Latest revision as of 01:40, 18 October 2018
Processing of experiment results
1. Verification of Poisson distribution
We infect tumor cells (HeLa GSDMD KO cell line) with Salmonella carrying the GFP gene for a period of time and then count them, and the result is that the distribution of Salmonella in the tumor cells is consistent with a Poisson distribution.
Figure 1. Infection of HeLa GSDMD KO cells by Salmonella and the result of analysis.
The amounts of host cells and bacteria were measured in experiments.
The concentration of bacteria was calculated according to MOI (multiplicity of infection) and cell density.
We adjusted the parameter As to match the curve with the results of the Salmonella infection experiment. When As is
equal to 0.0003, the curve simulated by the mathematical model matches the experimental measurements.
Figure 2. Parameter fitting of the tumor cells infection experiments. Red points are experimental results.
The parameters ‘Nsal’ and ‘Tumor’ are already known before the experiment. When the parameter ‘As’ is 0.0003, the model results best fit the experimental results.
