Difference between revisions of "Team:HZAU-China/Attributions"

 
(48 intermediate revisions by 5 users not shown)
Line 91: Line 91:
 
             z-index: 1;
 
             z-index: 1;
 
             background-color: #EEEEEE;
 
             background-color: #EEEEEE;
−
            opacity: 0.96;
 
−
            filter: alpha(opacity=96);
 
 
             box-shadow: 0 5px 15px #CCCCCC;
 
             box-shadow: 0 5px 15px #CCCCCC;
 
             display: none;
 
             display: none;
Line 154: Line 152:
 
         }
 
         }
  
−
         .daohang a:hover span{
+
         .daohang a:hover span {
−
             transform: rotateY(180deg);          
+
             transform: rotateY(180deg);
−
             -webkit-transform: rotateY(180deg);          
+
             -webkit-transform: rotateY(180deg);
−
             -moz-transform: rotateY(180deg);          
+
             -moz-transform: rotateY(180deg);
−
             -o-transform: rotateY(180deg);          
+
             -o-transform: rotateY(180deg);
 
             -ms-transform: rotateY(180deg);
 
             -ms-transform: rotateY(180deg);
−
             transition: all 0.5s ease-in-out;          
+
             transition: all 0.5s ease-in-out;
−
             -webkit-transition: all 0.5s ease-in-out;          
+
             -webkit-transition: all 0.5s ease-in-out;
−
             -moz-transition: all 0.5s ease-in-out;          
+
             -moz-transition: all 0.5s ease-in-out;
 
             -o-transition: all 0.5s ease-in-out;
 
             -o-transition: all 0.5s ease-in-out;
 
         }
 
         }
Line 238: Line 236:
 
         }
 
         }
  
−
         @media screen and (max-width: 1000px) {
+
         @media screen and (max-width: 1200px) {
 
             .logo-daohang {
 
             .logo-daohang {
−
                 left: 5%;
+
                 left: 3%;
−
            }
+
−
 
+
−
            .daohang .caidan {
+
−
                right: 0;
+
 
             }
 
             }
  
 
             .daohangyi {
 
             .daohangyi {
−
                 font-size: 14px;
+
                 font-size: 12px;
 
             }
 
             }
 
         }
 
         }
Line 255: Line 249:
  
 
             .daohang .caidan {
 
             .daohang .caidan {
 +
                right: 0;
 
                 width: 100%;
 
                 width: 100%;
 
             }
 
             }
Line 272: Line 267:
 
             .daohangyi img {
 
             .daohangyi img {
 
                 display: none;
 
                 display: none;
 +
            }
 +
 +
            .daohang .longName .item:before {
 +
                width: 150px;
 +
                left: 20px;
 +
            }
 +
 +
            .daohang .longName .item:hover:after {
 +
                width: 150px;
 +
                left: 20px;
 
             }
 
             }
 
         }
 
         }
Line 295: Line 300:
 
             width: 80%;
 
             width: 80%;
 
             /* height: auto; */
 
             /* height: auto; */
−
             margin: 20px 20px 0 0;
+
             margin: 20px 40px 0 0;
 
             /* right: 2%; */
 
             /* right: 2%; */
 
             /* top: 90px; */
 
             /* top: 90px; */
Line 311: Line 316:
  
 
         .cebian {
 
         .cebian {
−
             width: 15%;
+
             width: 200px;
 
             /* height: 80vh; */
 
             /* height: 80vh; */
 
             float: left;
 
             float: left;
Line 320: Line 325:
 
             /* border:2px solid black */
 
             /* border:2px solid black */
 
             /* background-color: #323643 */
 
             /* background-color: #323643 */
 +
        }
 +
 +
        .zhengwen p{
 +
            text-align: justify !important;
 +
            font-family:  'Times New Roman',Helvetica,'Open Sans',  Arial, sans-serif !important;
 +
            color: #3B3B3B;
 +
            font-size: 26px !important;
 +
            padding-right: 20px;
 
         }
 
         }
  
Line 330: Line 343:
  
 
         .h1 {
 
         .h1 {
−
             height: 100px;
+
             height: 80px;
−
             line-height: 100px;
+
             line-height: 80px;
 
             font-weight: bold;
 
             font-weight: bold;
 +
            height:auto;
 
             font-family: 'Product Sans', sans-serif;
 
             font-family: 'Product Sans', sans-serif;
−
             font-size: 50px;
+
             font-size: 40px;
−
             color: black;
+
             color: #3B3B3B;
−
             border-bottom: 3px solid black;
+
             border-bottom: 2px solid #676767;
−
             /* margin: 0 2.4%; */
+
            margin-bottom: 20px;
 +
        }
 +
 
 +
        .h2 {
 +
            height: 40px;
 +
            line-height: 40px;
 +
            font-weight: bold;
 +
            height:auto;
 +
            /* font-weight: bold; */
 +
            font-family: 'Product Sans', sans-serif;
 +
            font-size: 30px;
 +
            color: #484848;
 +
             /* margin-bottom: 20px; */
 +
        }
 +
 
 +
        .h3 {
 +
            height: 30px;
 +
            line-height: 30px;
 +
            font-weight: bold;
 +
            height:auto;
 +
            /* font-weight: bold; */
 +
            font-family: 'Product Sans', sans-serif;
 +
            font-size: 24px;
 +
            color: #484848;
 +
            /* margin-bottom: 20px; */
 +
        }
 +
       
 +
        table {
 +
            color: #3B3B3B;
 
         }
 
         }
  
Line 453: Line 495:
 
     <style>
 
     <style>
 
         #float01 {}
 
         #float01 {}
−
 
+
   
−
        #float02 {}
+
            #float02 {}
−
 
+
   
−
        #float03 {}
+
            div.floatCtro {
−
 
+
                width: 100%;
−
        div.floatCtro {
+
                /* height: auto; */
−
            width: 100%;
+
                margin: 0;
−
            height: 250px;
+
                position: relative;
−
            margin: 0;
+
                background: #fff;
−
            position: relative;
+
   
−
            background: #fff;
+
             }
−
 
+
   
−
        }
+
−
 
+
−
        .daohangyi {
+
−
            display: block;
+
−
            width: 100%;
+
−
            height: 40px;
+
−
            color: #ffffff !important;
+
−
            font-size: 16px;
+
−
            background-color: #323643;
+
−
            border-bottom: 1px solid black;
+
−
            /* border-radius: 5px; */
+
−
            text-decoration: none !important;
+
−
        }
+
−
 
+
−
        div.floatCtro .daohanger {
+
−
            width: 100%;
+
−
            text-align: justify !important;
+
−
            height: 45px;
+
−
            line-height: 45px;
+
−
            font-family: Arial;
+
−
            font-size: 14px;
+
−
            color: #676767;
+
−
            margin: 0;
+
−
            padding-left: 8%;
+
−
            cursor: pointer;
+
−
            background: #fff;
+
−
        }
+
−
 
+
−
        div.floatCtro a {
+
−
             display: inline-block;
+
−
            display: none;
+
−
            width: 100%;
+
−
            height: 40px;
+
−
            /* margin: 10px 0 0 0; */
+
−
            background: #FFF;
+
−
            color: #000 !important;
+
−
            vertical-align: middle;
+
−
            cursor: pointer;
+
−
        }
+
−
 
+
−
        div.floatCtro a span {
+
−
            display: block;
+
−
            height: 44px;
+
−
            line-height: 44px;
+
−
            font-family: Arial;
+
−
            font-size: 16px;
+
−
            padding-left: 8%;
+
−
        }
+
−
 
+
−
        div.floatCtro a:hover {
+
−
            /* background: #76b39d; */
+
−
            color: #EA0D04 !important;
+
−
            zoom: 1;
+
−
        }
+
−
 
+
−
        /* .cebian a:hover {
+
−
            color: #f6eee0 !important;
+
−
        } */
+
−
 
+
−
        div.floatCtro .daohanger:hover {
+
−
            /* background: #76b39d; */
+
−
            color: #1fa67a;
+
−
            text-decoration: underline !important;
+
−
        }
+
−
 
+
−
        div.floatCtro .daohanger.cur {
+
−
            /* background: #1fa67a; */
+
−
            color: #1fa67a;
+
−
        }
+
−
 
+
−
        .biaoti {
+
−
            line-height: 40px;
+
−
            width: 50%;
+
−
            margin-left: 6%;
+
−
            text-align: center;
+
−
            text-decoration: none !important;
+
−
        }
+
−
 
+
−
        .daohangyi img {
+
−
            display: inline-block;
+
−
            width: 12px;
+
−
            height: 12px;
+
−
            vertical-align: middle;
+
−
            background-size: 100% 100%;
+
−
 
+
−
        }
+
−
        .cebian div:hover img{
+
−
            transform: rotateY(180deg);           
+
−
            -webkit-transform: rotateY(180deg);           
+
−
            -moz-transform: rotateY(180deg);           
+
−
            -o-transform: rotateY(180deg);           
+
−
            -ms-transform: rotateY(180deg);
+
−
            transition: all 0.5s ease-in-out;           
+
−
            -webkit-transition: all 0.5s ease-in-out;           
+
−
            -moz-transition: all 0.5s ease-in-out;           
+
−
            -o-transition: all 0.5s ease-in-out;
+
−
        }
+
−
        .cebian a:hover img{
+
−
            transform: rotate(360deg);           
+
−
            -webkit-transform: rotate(360deg);           
+
−
            -moz-transform: rotate(360deg);           
+
−
            -o-transform: rotate(360deg);           
+
−
            -ms-transform: rotate(360deg);
+
−
            transition: all 0.5s ease-in-out;           
+
−
            -webkit-transition: all 0.5s ease-in-out;           
+
−
            -moz-transition: all 0.5s ease-in-out;           
+
−
            -o-transition: all 0.5s ease-in-out;
+
−
        }
+
−
 
+
−
        @media screen and (max-width: 1000px) {           
+
−
         
+
 
             .daohangyi {
 
             .daohangyi {
 +
                display: block;
 +
                width: 100%;
 +
                /* height: auto; */
 +
                /* text-align: left !important; */
 +
                color: #ffffff !important;
 +
                font-size: 16px;
 +
                padding:0;
 +
                background-color: #323643;
 +
                border-bottom: 1px solid black;
 +
                padding:0 3%;
 +
                text-decoration: none !important;
 +
            }
 +
   
 +
            div.floatCtro .daohanger {
 +
                width: 100%;
 +
                text-align: left !important;
 +
                height: auto;
 +
                line-height: 25px;
 +
                font-family: Arial;
 
                 font-size: 14px;
 
                 font-size: 14px;
 +
                color: #676767;
 +
                margin: 0;
 +
                padding:10px 8%;
 +
                cursor: pointer;
 +
                background: #fff;
 
             }
 
             }
−
        }
+
   
−
 
+
             div.floatCtro a {
−
        @media screen and (max-width: 880px) {
+
                display: inline-block;
−
 
+
−
             .daohangyi img {
+
 
                 display: none;
 
                 display: none;
−
            } 
+
                width: 100%;
−
           
+
                height: 40px;
−
            .laoshi img {
+
                padding:10px 8%;
−
                 height: 30vh;
+
                 /* margin: 10px 0 0 0; */
 +
                background: #FFF;
 +
                color: #000 !important;
 +
                vertical-align: middle;
 +
                cursor: pointer;
 
             }
 
             }
−
        }
+
   
−
     </style>
+
            div.floatCtro a span {
 +
                display: block;
 +
                height: auto;
 +
                text-align: left !important;
 +
                line-height: 20px;
 +
                font-family: Arial;
 +
                font-size: 16px;
 +
            }
 +
      
 +
            div.floatCtro a:hover {
 +
                /* background: #76b39d; */
 +
                color: #EA0D04 !important;
 +
                zoom: 1;
 +
            }
 +
   
 +
            /* .cebian a:hover {
 +
                color: #f6eee0 !important;
 +
            } */
 +
   
 +
            div.floatCtro .daohanger:hover {
 +
                /* background: #76b39d; */
 +
                color: #1fa67a;
 +
                text-decoration: underline !important;
 +
            }
 +
   
 +
            div.floatCtro .daohanger.cur {
 +
                /* background: #1fa67a; */
 +
                color: #1fa67a;
 +
            }
 +
   
 +
            .biaoti {
 +
                line-height: 40px;
 +
                width: 50%;
 +
                /* margin-left: 6%; */
 +
                text-align: center;
 +
                text-decoration: none !important;
 +
            }
 +
   
 +
            .daohangyi img {
 +
                display: inline-block;
 +
                width: 12px;
 +
                height: 12px;
 +
                vertical-align: middle;
 +
                background-size: 100% 100%;
 +
   
 +
            }
 +
            .cebian div:hover img{
 +
                transform: rotateY(180deg);           
 +
                -webkit-transform: rotateY(180deg);           
 +
                -moz-transform: rotateY(180deg);           
 +
                -o-transform: rotateY(180deg);           
 +
                -ms-transform: rotateY(180deg);
 +
                transition: all 0.5s ease-in-out;           
 +
                -webkit-transition: all 0.5s ease-in-out;           
 +
                -moz-transition: all 0.5s ease-in-out;           
 +
                -o-transition: all 0.5s ease-in-out;
 +
            }
 +
            .cebian a:hover img{
 +
                transform: rotate(360deg);           
 +
                -webkit-transform: rotate(360deg);           
 +
                -moz-transform: rotate(360deg);           
 +
                -o-transform: rotate(360deg);           
 +
                -ms-transform: rotate(360deg);
 +
                transition: all 0.5s ease-in-out;           
 +
                -webkit-transition: all 0.5s ease-in-out;           
 +
                -moz-transition: all 0.5s ease-in-out;           
 +
                -o-transition: all 0.5s ease-in-out;
 +
            }
 +
   
 +
            @media screen and (max-width: 1200px) {           
 +
             
 +
                .cebian {
 +
                width: 15%;
 +
                }
 +
   
 +
                .zhengwen {
 +
                margin: 20px 20px 0 0;
 +
                }
 +
   
 +
            }
 +
   
 +
            @media screen and (max-width: 900px) {
 +
   
 +
                .daohangyi img {
 +
                    display: none;
 +
                } 
 +
               
 +
                .daohanger {
 +
                font-size: 12px !important;
 +
                }
 +
 
 +
                .laoshi img {
 +
                    height: 30vh;
 +
                }
 +
            }
 +
        </style>
  
  
Line 603: Line 655:
 
         <ul class="caidan">
 
         <ul class="caidan">
 
             <li class="hiLight shortName">
 
             <li class="hiLight shortName">
−
                 <a class="nav_head" href="#">
+
                 <a class="nav_head" href="https://2018.igem.org/Team:HZAU-China">
 
                     <span>Project</span>
 
                     <span>Project</span>
−
                     <span class="xjtPic"></span>
+
                     <span class="xsjPic"></span>
 
                 </a>
 
                 </a>
 
                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/Description">Description</a>
 
                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/Description">Description</a>
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                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/Experiments">Experiments</a>
 
                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/Experiments">Experiments</a>
 
                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/Improve">Improve</a>
 
                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/Improve">Improve</a>
−
                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/InterLab">Interlab</a>
+
                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/InterLab">InterLab</a>
 +
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Notebook">Notebook</a>
 +
 
 
             </li>
 
             </li>
 
             <li class="hiLight shortName">
 
             <li class="hiLight shortName">
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                     <span class="xjtPic"></span>
 
                     <span class="xjtPic"></span>
 
                 </a>
 
                 </a>
−
                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/Safety">Safty</a>
+
                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/Safety">Safety</a>
 
                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/Human_Practices">Human Practices</a>
 
                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/Human_Practices">Human Practices</a>
 
                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/Public_Engagement">Public Engagement</a>
 
                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/Public_Engagement">Public Engagement</a>
Line 647: Line 701:
 
                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/Collaborations">Collaborations</a>
 
                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/Collaborations">Collaborations</a>
 
             </li>
 
             </li>
−
             <li class="shortName">
+
             <li class="hiLight shortName">
−
                 <a class="nav_head" href="https://2018.igem.org/Team:HZAU-China/Parts">
+
                 <a class="nav_head" href="#">
 
                     <span>Parts</span>
 
                     <span>Parts</span>
 +
                    <span class="xjtPic"></span>
 
                 </a>
 
                 </a>
 +
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Basic_Part">Basic</a>
 +
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Composite_Part">Composite</a>
 
             </li>
 
             </li>
 
         </ul>
 
         </ul>
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         <!-- 正文 -->
 
         <!-- 正文 -->
 
         <div class="zhengwen">
 
         <div class="zhengwen">
−
            <img class="daimg" src="https://static.igem.org/mediawiki/2018/7/70/T--HZAU-China--hezhao1.png" alt="">
 
 
             <div id="float01" class="cur">
 
             <div id="float01" class="cur">
−
                 <div class="h1">biaotiyi</div>
+
                 <div class="h1">Attributions</div>
−
 
+
                <p>Our project was designed by <b>Zhujun Xia</b> and all circuits were designed by <b>Zhujun Xia</b>, <b>YinQing
 +
                        Zeng</b> and
 +
                    <b>Mo Qiqin</b>.</p>
 +
                <div class="h2">Wet Lab</div>
 +
                <div class="h3">Gene Editing</div>
 +
                <p><i>sifA</i> was knocked out by <b>Zhujun Xia</b>, <b>Zhuoqi Huang</b> and <b>Wenxin Hu</b>
 +
                    also gave
 +
                    assistance.</p>
 +
                <div class="h3">Plasmid Construction</div>
 +
                <p><b>Intracellular Environment-Dependent Circuits:</b></p>
 +
                <p>The intracellular environment-dependent circuits were designed by <b>Mo Qiqin</b> and <b>Zhujun Xia</b>.</p>
 +
                <p>The intracellular environment-dependent circuits were constructed by <b>Mo Qiqin</b>. <b>Ruonan Tian</b>
 +
                    also gave
 +
                    assistance.</p>
 +
                <br>
 +
                <p><b>Chemical Control System:</b></p>
 +
                <p>The ATc induction chemical control system was constructed by <b>He Yang</b> and <b>Zhuoqi Huang</b> also
 +
                    gave
 +
                    assistance.</p>
 +
                <p>The TetR-eGFP fusion protein expression system for TetR expression measurement was constructed by <b>He
 +
                        Yang</b> and <b>Zhuoqi Huang</b> also gave assistance.</p>
 +
                <br>
 +
                <p><b>Targeting Specificity and Surface-Displaying:</b></p>
 +
                <p>RGD-inserted OmpA circuit clone and construction of <i>Salmonella</i> SL1344_RS05255 (OmpA coding
 +
                    sequence) were completed by <b>YinQing Zeng</b> and <b>Lingyu Zhong</b>.</p>
 +
                <p>The circuit of surface-display system was constructed by <b>Lingyu Zhong</b>.
 +
                    Optimation of OmpA in <i>E. Coli</i> was completed by <b>Zhujun Xia</b>.</p>
 +
                <br>
 +
                <p><b>Plasmid Standardization:</b></p>
 +
                <p>Plasmid standardization was completed by <b>Zhiqing Guo</b>, <b>Hangxi Fu</b> and <b>Zhuoqi Huang</b> by using 3A
 +
                    Assembly.
 +
                </p>
 +
                <br>
 +
                <p><b>Plasmid Submission:</b></p>
 +
                <p>Submitted plasmids (Pcs2-eGFP-GSDMD FL, Pcs2-eGFP-GSDMD-N275 and two GSDMD full length mutations)
 +
                    were constructed by <b>Zhiqing Guo</b> and <b>Wenxin Hu</b>.</p>
 +
                <p>The description of submitted plasmids by ATP assay and cell phenotype via transfection was designed
 +
                    and completed by <b>Zhujun Xia</b>.
 +
                </p>
 +
                <div class="h3">Bacterial Phenotype Verification</div>
 +
                <p>Bacterial phenotype verifications were designed and completed by <b>He Yang</b>, including the OD
 +
                    measurement
 +
                    and CFU measurement of chemical control system.</p>
 +
                <div class="h3">Cell Phenotype Verification</div>
 +
                <p>Cell phenotype verifications were designed and completed by <b>Zhujun Xia</b>, including the
 +
                    verification
 +
                    of chemical control system, and targeting specificity, all the related circuits and the demonstration of the final work system.</p>
 +
                <div class="h3">Biosafety Verification</div>
 +
                <p>Safety verification was designed and completed by <b>Zhujun Xia</b>, which demonstrated our project
 +
                    is safe
 +
                    enough to be used.</p>
 +
                <div class="h3">InterLab</div>
 +
                <p>InterLab was conducted by <b>Xichen Rao</b>, <b>Zhujun Xia</b>, <b>Ruonan Tian</b> and <b>Heng Heng</b>
 +
                    also gave assistance.
 +
                    Our data passed the check of the Measurement Committee successfully.</p>
 +
                <div class="h2">Dry Lab</div>
 +
                <p>Mathematical model and app about salmonella infection and GSDMD release were built by <b>Songtao
 +
                        Chen</b>.</p>
 +
                    <p>ATc model which predicted the relationship between concentration of ATc and concentration of GSDMD
 +
                    was built by <b>Xichen Rao</b>.</p>
 +
                    <p>The modeling on adjusting the strength of RBS and ATc with fixed GSDMD-N concentration was completed
 +
                    by <b>Yini Miao</b>. </p>
 +
                    <p>The modeling of the promoter sifA and promoter ent was completed by <b>Ruonan Tian</b>.
 +
                </p>
 +
                <div class="h2">Software</div>
 +
                <p>The applet of "Bacterial Colony Counter" was designed and programmed by <b>Shuguang Wang</b>.</p>
 +
                <div class="h2">Human Practice</div>
 +
                <p>Collaboration and communication with other teams were completed by <b>Yinqing Zeng</b> and all the other team members.</p>
 +
                    <p>Handicraft manufacture was designed and completed by <b>Yinqing Zeng</b>.</p>
 +
                    <p>Questionnaire preparation, the advertisement of synthetic biology and preparation of popular science articles
 +
                    were completed by <b>Heng Heng</b>.</p>
 +
                    <p>Two posters for Eurasian exchange meetings and CCIC were completed by <b>Ruonan Tian</b>.
 +
                </p>
 +
                <div class="h2">Website</div>
 +
                <p>Our wiki framework was designed and programmed by <b>Shuguang Wang</b> and our artist were
 +
                    <b>Yuying Xiang</b> and <b>Kening Chen</b>.</p>
 +
                <p>Our <a href="https://wang-shuguang.github.io/">blog</a> was built by <b>Shuguang Wang</b>.</p>
 +
                <div class="h2">Others</div>
 +
                <p>The plate reader machine operation was conducted by <b>Mo Qiqin</b>.</p>
 +
                <p>Chief cleaner of the laboratory was <b>Xichen Rao</b>.
 +
                </p>
 
             </div>
 
             </div>
 
             <div id="float02">
 
             <div id="float02">
−
                 <div class="h1">biaotier</div>
+
                 <div class="h1">Acknowledgements</div>
−
 
+
                <p>For each parts of experiments, our appreciations are as follow:</p>
−
            </div>
+
                <div class="h2">Cell lines</div>
−
            <div id="float03">
+
                <p>
−
                 <div class="h1">biaotisan</div>
+
                    Thanks to Prof. <b>Shan Li</b>’s laboratory, Huazhong Agricultural University, for sharing the cell
 +
                    lines
 +
                    of HEK293T, HELA, MCF7, iBMDM, so that our experiment can process successfully.
 +
                </p>
 +
                <p> Thanks to Dr. <b>Feng Shao</b>’s laboratory, for sharing the cell line of GSDMD<i>-/-</i> HELA cells, which
 +
                    provides
 +
                    us great support.
 +
                </p>
 +
                <p> Thanks to Dr. <b>Yi Zeng</b> and Dr. Ms <b>Hong Fan</b>, Shanghai Institute of Biochemistry and
 +
                    Cell Biology,
 +
                    CAS, for sharing the cell line of MDA-MB-231.
 +
                </p>
 +
                <div class="h2">Bacteria</div>
 +
                <p>Thanks to Prof. <b>Shan Li</b>’s laboratory, Huazhong Agricultural University, for sharing the
 +
                    strain of
 +
                    <i>Salmonella enterica</i> var. Typhimurium SL1344.
 +
                </p>
 +
                <p> Thanks to Dr. <b>Chenli Liu</b> from SIAT CSynBER for generously sharing the material (Strain: <i>E.coli</i> K-12 MG1655 with
 +
                    mCherry in the genome).
 +
                </p>
 +
                 <div class="h2">Gene Knock In and Knock Out</div>
 +
                <p>Thanks to Prof. <b>Shan Li</b>’s laboratory, Huazhong Agricultural University, for providing the
 +
                    protocol
 +
                    and material of gene editing based on two-step allelic exchange, which provides us with great help.
 +
                </p>
 
                  
 
                  
 +
                <div class="h2">Plasmid Backbone</div>
 +
                <p>Thanks to Prof. <b>Shan Li</b>’s laboratory, Huazhong Agricultural University, for sharing the
 +
                    plasmid
 +
                    backbone (Pcs2-eGFP) to us.</p>
 +
                <div class="h2">Sponsors</div>
 +
                <p>Thanks to <b>GeneScript</b> and <b>IDT</b> for DNA sequence synthesis service.</p>
 +
                <p>Thanks to <b>SnapGene</b> and <b>MathWorks</b> for free software supporting.</p>
 +
                <div class="row">
 +
                    <div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
 +
                        <img src="https://static.igem.org/mediawiki/2018/c/cd/T--HZAU-China--GenScript.png" width=100% alt="">
 +
                    </div>
 +
                    <div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
 +
                        <img src="https://static.igem.org/mediawiki/2018/6/6a/T--HZAU-China--IDT.png" width=100% alt="">
 +
                    </div>
 +
                </div>
 +
                <div class="row">
 +
                    <div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
 +
                        <img src="https://static.igem.org/mediawiki/2018/0/0e/T--HZAU-China--SnapGene.png" width=100% alt="">
 +
                    </div>
 +
                    <div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
 +
                        <img src="https://static.igem.org/mediawiki/2018/5/5d/T--HZAU-China--MathWorks.png" width=100% alt="">
 +
                    </div>
 +
                </div>
 +
                <br>
 +
                <br>
 +
                <br>
 +
                <p>Thanks to <b>College of Life Science and Technology and Huazhong Agricultural University</b> for providing
 +
                    the working site and fund for us.
 +
                </p>
 +
                <div style="width: 50%; margin: 10px auto">
 +
                    <img src="https://static.igem.org/mediawiki/2018/1/1e/T--HZAU-China--huanong.png" width=100% alt="">
 +
                </div>
 +
                <p> Thanks to Prof. <b>Binguang Ma</b> as our Primary PI and for supervising the whole project and instructing our modeling.
 +
                </p>
 +
                <p> Thanks to Prof. <b>Jin He</b> as our Secondary PI and Prof. <b>Shan Li</b> for instructing our
 +
                    experiment.
 +
                </p>
 +
                <p> Thanks to Prof. <b>Jin He</b>, Prof. <b>Shan Li</b>, Prof. <b>Gang Cao</b>, Prof. <b>Donghai Peng</b>
 +
                    and Prof. <b>Ming Sun</b> for
 +
                    giving us advices at the proposal presentation.
 +
                </p>
 +
                <p> Thanks to <b>Kening Chen</b>, <b>Wenqi Huang</b> and <b>Haimeng Li</b> from iGEM-2017, Huazhong
 +
                    Agricultural University,
 +
                    for giving us advices.
 +
                </p>
 +
                <p> Thanks to <b>Weitong Guo</b> from iGEM-2017, Huazhong Agricultural University, for the training of
 +
                    new dry-labers during winter vacation.
 +
                <p style="margin-bottom:30px"> We really appreciate your support!
 +
                </p>
 +
 
             </div>
 
             </div>
 
         </div>
 
         </div>
Line 681: Line 891:
 
             </a>
 
             </a>
 
             <div class="daohangyi">
 
             <div class="daohangyi">
−
                    <span class="biaoti">Attributions</span>
+
                <span class="biaoti">Attributions</span>
−
                    <span class="xsjPic"><img src="https://static.igem.org/mediawiki/2018/8/8d/T--HZAU-China--xjt.svg" alt=""></span>
+
                <span class="xsjPic"><img src="https://static.igem.org/mediawiki/2018/8/8d/T--HZAU-China--xjt.svg" alt=""></span>
−
                </div>
+
            </div>
 
             <div class="floatCtro">
 
             <div class="floatCtro">
−
                 <p class="daohanger">neirongyi</p>
+
                 <p class="daohanger">Attributions</p>
−
                 <p class="daohanger">neironger</p>
+
                 <p class="daohanger">Acknowledgement</p>
−
                <p class="daohanger">neirongsan</p>
+
 
                 <a>
 
                 <a>
 
                     <span>Back&nbsp;to&nbsp;Top</span>
 
                     <span>Back&nbsp;to&nbsp;Top</span>
Line 721: Line 930:
 
             scrolls()
 
             scrolls()
 
             function scrolls() {
 
             function scrolls() {
−
                 var f1, f2, f3, bck;
+
                 var f1, f2, bck;
 
                 var fixRight = $('div.floatCtro p');
 
                 var fixRight = $('div.floatCtro p');
 
                 var blackTop = $('div.floatCtro a')
 
                 var blackTop = $('div.floatCtro a')
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                 fl = $('#float01').offset().top;
 
                 fl = $('#float01').offset().top;
 
                 f2 = $('#float02').offset().top;
 
                 f2 = $('#float02').offset().top;
−
                f3 = $('#float03').offset().top;
 
  
 
                 if (sTop <= f2 - 100) {
 
                 if (sTop <= f2 - 100) {
Line 741: Line 949:
 
                 if (sTop >= f2 - 100) {
 
                 if (sTop >= f2 - 100) {
 
                     fixRight.eq(1).addClass('cur').siblings().removeClass('cur');
 
                     fixRight.eq(1).addClass('cur').siblings().removeClass('cur');
−
                }
 
−
                if (sTop >= f3 - 100) {
 
−
                    fixRight.eq(2).addClass('cur').siblings().removeClass('cur');
 
 
                 }
 
                 }
  

Latest revision as of 02:00, 18 October 2018

Attributions

Our project was designed by Zhujun Xia and all circuits were designed by Zhujun Xia, YinQing Zeng and Mo Qiqin.

Wet Lab
Gene Editing

sifA was knocked out by Zhujun Xia, Zhuoqi Huang and Wenxin Hu also gave assistance.

Plasmid Construction

Intracellular Environment-Dependent Circuits:

The intracellular environment-dependent circuits were designed by Mo Qiqin and Zhujun Xia.

The intracellular environment-dependent circuits were constructed by Mo Qiqin. Ruonan Tian also gave assistance.


Chemical Control System:

The ATc induction chemical control system was constructed by He Yang and Zhuoqi Huang also gave assistance.

The TetR-eGFP fusion protein expression system for TetR expression measurement was constructed by He Yang and Zhuoqi Huang also gave assistance.


Targeting Specificity and Surface-Displaying:

RGD-inserted OmpA circuit clone and construction of Salmonella SL1344_RS05255 (OmpA coding sequence) were completed by YinQing Zeng and Lingyu Zhong.

The circuit of surface-display system was constructed by Lingyu Zhong. Optimation of OmpA in E. Coli was completed by Zhujun Xia.


Plasmid Standardization:

Plasmid standardization was completed by Zhiqing Guo, Hangxi Fu and Zhuoqi Huang by using 3A Assembly.


Plasmid Submission:

Submitted plasmids (Pcs2-eGFP-GSDMD FL, Pcs2-eGFP-GSDMD-N275 and two GSDMD full length mutations) were constructed by Zhiqing Guo and Wenxin Hu.

The description of submitted plasmids by ATP assay and cell phenotype via transfection was designed and completed by Zhujun Xia.

Bacterial Phenotype Verification

Bacterial phenotype verifications were designed and completed by He Yang, including the OD measurement and CFU measurement of chemical control system.

Cell Phenotype Verification

Cell phenotype verifications were designed and completed by Zhujun Xia, including the verification of chemical control system, and targeting specificity, all the related circuits and the demonstration of the final work system.

Biosafety Verification

Safety verification was designed and completed by Zhujun Xia, which demonstrated our project is safe enough to be used.

InterLab

InterLab was conducted by Xichen Rao, Zhujun Xia, Ruonan Tian and Heng Heng also gave assistance. Our data passed the check of the Measurement Committee successfully.

Dry Lab

Mathematical model and app about salmonella infection and GSDMD release were built by Songtao Chen.

ATc model which predicted the relationship between concentration of ATc and concentration of GSDMD was built by Xichen Rao.

The modeling on adjusting the strength of RBS and ATc with fixed GSDMD-N concentration was completed by Yini Miao.

The modeling of the promoter sifA and promoter ent was completed by Ruonan Tian.

Software

The applet of "Bacterial Colony Counter" was designed and programmed by Shuguang Wang.

Human Practice

Collaboration and communication with other teams were completed by Yinqing Zeng and all the other team members.

Handicraft manufacture was designed and completed by Yinqing Zeng.

Questionnaire preparation, the advertisement of synthetic biology and preparation of popular science articles were completed by Heng Heng.

Two posters for Eurasian exchange meetings and CCIC were completed by Ruonan Tian.

Website

Our wiki framework was designed and programmed by Shuguang Wang and our artist were Yuying Xiang and Kening Chen.

Our blog was built by Shuguang Wang.

Others

The plate reader machine operation was conducted by Mo Qiqin.

Chief cleaner of the laboratory was Xichen Rao.

Acknowledgements

For each parts of experiments, our appreciations are as follow:

Cell lines

Thanks to Prof. Shan Li’s laboratory, Huazhong Agricultural University, for sharing the cell lines of HEK293T, HELA, MCF7, iBMDM, so that our experiment can process successfully.

Thanks to Dr. Feng Shao’s laboratory, for sharing the cell line of GSDMD-/- HELA cells, which provides us great support.

Thanks to Dr. Yi Zeng and Dr. Ms Hong Fan, Shanghai Institute of Biochemistry and Cell Biology, CAS, for sharing the cell line of MDA-MB-231.

Bacteria

Thanks to Prof. Shan Li’s laboratory, Huazhong Agricultural University, for sharing the strain of Salmonella enterica var. Typhimurium SL1344.

Thanks to Dr. Chenli Liu from SIAT CSynBER for generously sharing the material (Strain: E.coli K-12 MG1655 with mCherry in the genome).

Gene Knock In and Knock Out

Thanks to Prof. Shan Li’s laboratory, Huazhong Agricultural University, for providing the protocol and material of gene editing based on two-step allelic exchange, which provides us with great help.

Plasmid Backbone

Thanks to Prof. Shan Li’s laboratory, Huazhong Agricultural University, for sharing the plasmid backbone (Pcs2-eGFP) to us.

Sponsors

Thanks to GeneScript and IDT for DNA sequence synthesis service.

Thanks to SnapGene and MathWorks for free software supporting.




Thanks to College of Life Science and Technology and Huazhong Agricultural University for providing the working site and fund for us.

Thanks to Prof. Binguang Ma as our Primary PI and for supervising the whole project and instructing our modeling.

Thanks to Prof. Jin He as our Secondary PI and Prof. Shan Li for instructing our experiment.

Thanks to Prof. Jin He, Prof. Shan Li, Prof. Gang Cao, Prof. Donghai Peng and Prof. Ming Sun for giving us advices at the proposal presentation.

Thanks to Kening Chen, Wenqi Huang and Haimeng Li from iGEM-2017, Huazhong Agricultural University, for giving us advices.

Thanks to Weitong Guo from iGEM-2017, Huazhong Agricultural University, for the training of new dry-labers during winter vacation.

We really appreciate your support!

Team
Attributions

Attributions

Acknowledgement

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