Difference between revisions of "Team:HZAU-China/Attributions"

 
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             <div id="float01" class="cur">
 
             <div id="float01" class="cur">
 
                 <div class="h1">Attributions</div>
 
                 <div class="h1">Attributions</div>
−
                 <p>Our project is designed by <b>Zhujun Xia</b> and all circuits is designed by <b>Zhujun Xia</b>, <b>YinQing
+
                 <p>Our project was designed by <b>Zhujun Xia</b> and all circuits were designed by <b>Zhujun Xia</b>, <b>YinQing
 
                         Zeng</b> and
 
                         Zeng</b> and
 
                     <b>Mo Qiqin</b>.</p>
 
                     <b>Mo Qiqin</b>.</p>
 
                 <div class="h2">Wet Lab</div>
 
                 <div class="h2">Wet Lab</div>
 
                 <div class="h3">Gene Editing</div>
 
                 <div class="h3">Gene Editing</div>
−
                 <p><i>sifA</i> and <i>sipD</i> knock out is completed by <b>Zhujun Xia</b>, <b>Zhuoqi Huang</b> and <b>Wenxin Hu</b>
+
                 <p><i>sifA</i> was knocked out by <b>Zhujun Xia</b>, <b>Zhuoqi Huang</b> and <b>Wenxin Hu</b>
 
                     also gave
 
                     also gave
 
                     assistance.</p>
 
                     assistance.</p>
 
                 <div class="h3">Plasmid Construction</div>
 
                 <div class="h3">Plasmid Construction</div>
 
                 <p><b>Intracellular Environment-Dependent Circuits:</b></p>
 
                 <p><b>Intracellular Environment-Dependent Circuits:</b></p>
−
                 <p>The intracellular environment-dependent circuits is designed by <b>Mo Qiqin</b> and <b>Zhujun Xia</b>.</p>
+
                 <p>The intracellular environment-dependent circuits were designed by <b>Mo Qiqin</b> and <b>Zhujun Xia</b>.</p>
−
                 <p>The intracellular environment-dependent circuits is constructed by <b>Mo Qiqin</b>. <b>Ruonan Tian</b>
+
                 <p>The intracellular environment-dependent circuits were constructed by <b>Mo Qiqin</b>. <b>Ruonan Tian</b>
 
                     also gave
 
                     also gave
 
                     assistance.</p>
 
                     assistance.</p>
 
                 <br>
 
                 <br>
 
                 <p><b>Chemical Control System:</b></p>
 
                 <p><b>Chemical Control System:</b></p>
−
                 <p>The ATc induced chemical control system is constructed by <b>He Yang</b>, <b>Zhuoqi Huang</b> also
+
                 <p>The ATc induction chemical control system was constructed by <b>He Yang</b> and <b>Zhuoqi Huang</b> also
 
                     gave
 
                     gave
 
                     assistance.</p>
 
                     assistance.</p>
−
                 <p>The TetR-eGFP fusion protein expression system for TetR expression measuring is constructed by <b>He
+
                 <p>The TetR-eGFP fusion protein expression system for TetR expression measurement was constructed by <b>He
−
                         Yang</b>, <b>Zhuoqi Huang</b> also gave assistance.</p>
+
                         Yang</b> and <b>Zhuoqi Huang</b> also gave assistance.</p>
 
                 <br>
 
                 <br>
 
                 <p><b>Targeting Specificity and Surface-Displaying:</b></p>
 
                 <p><b>Targeting Specificity and Surface-Displaying:</b></p>
 
                 <p>RGD-inserted OmpA circuit clone and construction of <i>Salmonella</i> SL1344_RS05255 (OmpA coding
 
                 <p>RGD-inserted OmpA circuit clone and construction of <i>Salmonella</i> SL1344_RS05255 (OmpA coding
−
                     sequence) are completed by <b>YinQing Zeng</b> and <b>Lingyu Zhong</b>.</p>
+
                     sequence) were completed by <b>YinQing Zeng</b> and <b>Lingyu Zhong</b>.</p>
−
                 <p>The circuit of surface-display system is constructed by <b>Lingyu Zhong</b>.
+
                 <p>The circuit of surface-display system was constructed by <b>Lingyu Zhong</b>.
−
                     Optimation of OmpA in <i>E. Coli</i> is completed by <b>Zhujun Xia</b>.</p>
+
                     Optimation of OmpA in <i>E. Coli</i> was completed by <b>Zhujun Xia</b>.</p>
 
                 <br>
 
                 <br>
 
                 <p><b>Plasmid Standardization:</b></p>
 
                 <p><b>Plasmid Standardization:</b></p>
−
                 <p>Plasmid standardization is completed by <b>Zhiqing Guo</b>, <b>Zhuoqi Huang</b> by using 3A
+
                 <p>Plasmid standardization was completed by <b>Zhiqing Guo</b>, <b>Hangxi Fu</b> and <b>Zhuoqi Huang</b> by using 3A
 
                     Assembly.
 
                     Assembly.
 
                 </p>
 
                 </p>
 
                 <br>
 
                 <br>
 
                 <p><b>Plasmid Submission:</b></p>
 
                 <p><b>Plasmid Submission:</b></p>
−
                 <p>Submitted plasmids (Pcs2-eGFP-GSDMD FL, Pcs2-eGFP-GSDMD-N275 and three GSDMD full length mutations)
+
                 <p>Submitted plasmids (Pcs2-eGFP-GSDMD FL, Pcs2-eGFP-GSDMD-N275 and two GSDMD full length mutations)
−
                     are constructed by <b>Zhiqing Guo</b> and <b>Wenxin Hu</b>.</p>
+
                     were constructed by <b>Zhiqing Guo</b> and <b>Wenxin Hu</b>.</p>
−
                 <p>The description of submitted plasmids by ATP assay and cell phenotype via transfection is designed
+
                 <p>The description of submitted plasmids by ATP assay and cell phenotype via transfection was designed
 
                     and completed by <b>Zhujun Xia</b>.
 
                     and completed by <b>Zhujun Xia</b>.
 
                 </p>
 
                 </p>
 
                 <div class="h3">Bacterial Phenotype Verification</div>
 
                 <div class="h3">Bacterial Phenotype Verification</div>
−
                 <p>Bacterial phenotype verification are designed and completed by <b>He Yang</b>, including the OD
+
                 <p>Bacterial phenotype verifications were designed and completed by <b>He Yang</b>, including the OD
 
                     measurement
 
                     measurement
 
                     and CFU measurement of chemical control system.</p>
 
                     and CFU measurement of chemical control system.</p>
 
                 <div class="h3">Cell Phenotype Verification</div>
 
                 <div class="h3">Cell Phenotype Verification</div>
−
                 <p>Cell phenotype verifications are designed and completed by <b>Zhujun Xia</b>, including the
+
                 <p>Cell phenotype verifications were designed and completed by <b>Zhujun Xia</b>, including the
 
                     verification
 
                     verification
−
                     of chemical control system, and targeting specificity, all these circuits and systems work so well.</p>
+
                     of chemical control system, and targeting specificity, all the related circuits and the demonstration of the final work system.</p>
−
                 <div class="h3">Bio-Safety Verification</div>
+
                 <div class="h3">Biosafety Verification</div>
−
                 <p>Safety verification is designed and completed by <b>Zhujun Xia</b>, which demonstrated our project
+
                 <p>Safety verification was designed and completed by <b>Zhujun Xia</b>, which demonstrated our project
 
                     is safe
 
                     is safe
 
                     enough to be used.</p>
 
                     enough to be used.</p>
 
                 <div class="h3">InterLab</div>
 
                 <div class="h3">InterLab</div>
−
                 <p>InterLab is conducted by <b>Xichen Rao</b>, <b>Zhujun Xia</b>, <b>Ruonan Tian</b> and <b>Heng Heng</b>
+
                 <p>InterLab was conducted by <b>Xichen Rao</b>, <b>Zhujun Xia</b>, <b>Ruonan Tian</b> and <b>Heng Heng</b>
−
                     also gave assistance,
+
                     also gave assistance.
−
                     our data passed the check of the Measurement Committee successfully.</p>
+
                     Our data passed the check of the Measurement Committee successfully.</p>
 
                 <div class="h2">Dry Lab</div>
 
                 <div class="h2">Dry Lab</div>
−
                 <p>Mathematical model and app of salmonella infection and the releasing of GSDMD are built by <b>Songtao
+
                 <p>Mathematical model and app about salmonella infection and GSDMD release were built by <b>Songtao
−
                         Chen</b>.
+
                         Chen</b>.</p>
−
                     ATc model which predicted the relationship between concentration of ATc and concentration of GSDMD
+
                     <p>ATc model which predicted the relationship between concentration of ATc and concentration of GSDMD
−
                     is built by <b>Xichen Rao</b>.
+
                     was built by <b>Xichen Rao</b>.</p>
−
                     The modeling of adjusting the strength of RBS and ATc with fixed GSDMD-N concentration is completed
+
                     <p>The modeling on adjusting the strength of RBS and ATc with fixed GSDMD-N concentration was completed
−
                     by <b>Yini Miao</b>. The future work is confirming the threshold of GSDMD-N275.
+
                     by <b>Yini Miao</b>. </p>
−
                     The modeling of the promoter sifA and promoter entC is completed by <b>Ruonan Tian</b>.
+
                     <p>The modeling of the promoter sifA and promoter ent was completed by <b>Ruonan Tian</b>.
 
                 </p>
 
                 </p>
 
                 <div class="h2">Software</div>
 
                 <div class="h2">Software</div>
−
                 <p>The applet of "Bacterial Colony Counter" is designed and programmed by <b>Shuguang Wang</b>.</p>
+
                 <p>The applet of "Bacterial Colony Counter" was designed and programmed by <b>Shuguang Wang</b>.</p>
 
                 <div class="h2">Human Practice</div>
 
                 <div class="h2">Human Practice</div>
−
                 <p>Collaboration and communication with other teams is completed by <b>YinQing Zeng</b>.
+
                 <p>Collaboration and communication with other teams were completed by <b>Yinqing Zeng</b> and all the other team members.</p>
−
                     Handicraft manufacture is designed and completed by <b>YinQing Zeng</b>.
+
                     <p>Handicraft manufacture was designed and completed by <b>Yinqing Zeng</b>.</p>
−
                     Questionnaire preparation, the advertisement of synthetic biology and popular science articles
+
                     <p>Questionnaire preparation, the advertisement of synthetic biology and preparation of popular science articles
−
                     written is completed by <b>Heng Heng</b>.
+
                     were completed by <b>Heng Heng</b>.</p>
−
                     Two posters for Eurasian exchange meetings and CCIC is completed by <b>Ruonan Tian</b>.
+
                     <p>Two posters for Eurasian exchange meetings and CCIC were completed by <b>Ruonan Tian</b>.
 
                 </p>
 
                 </p>
 
                 <div class="h2">Website</div>
 
                 <div class="h2">Website</div>
−
                 <p>Our intergrative wiki is designed and programmed by <b>Shuguang Wang</b> and the artist is designed
+
                 <p>Our wiki framework was designed and programmed by <b>Shuguang Wang</b> and our artist were
−
                     by <b>Yuying Xiang</b>.</p>
+
                     <b>Yuying Xiang</b> and <b>Kening Chen</b>.</p>
−
                 <p>Our <a href="https://wang-shuguang.github.io/">blog</a> is built by <b>Shuguang Wang</b>.</p>
+
                 <p>Our <a href="https://wang-shuguang.github.io/">blog</a> was built by <b>Shuguang Wang</b>.</p>
 
                 <div class="h2">Others</div>
 
                 <div class="h2">Others</div>
−
                 <p>The plate reader machine operation is conducted by <b>Mo Qiqin</b>.
+
                 <p>The plate reader machine operation was conducted by <b>Mo Qiqin</b>.</p>
−
                    Chief cleaner of the laboratory is <b>Xichen Rao</b>.
+
                <p>Chief cleaner of the laboratory was <b>Xichen Rao</b>.
 
                 </p>
 
                 </p>
 
             </div>
 
             </div>
 
             <div id="float02">
 
             <div id="float02">
−
                 <div class="h1">Acknowledgement</div>
+
                 <div class="h1">Acknowledgements</div>
 
                 <p>For each parts of experiments, our appreciations are as follow:</p>
 
                 <p>For each parts of experiments, our appreciations are as follow:</p>
 
                 <div class="h2">Cell lines</div>
 
                 <div class="h2">Cell lines</div>
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                     Thanks to Prof. <b>Shan Li</b>’s laboratory, Huazhong Agricultural University, for sharing the cell
 
                     Thanks to Prof. <b>Shan Li</b>’s laboratory, Huazhong Agricultural University, for sharing the cell
 
                     lines
 
                     lines
−
                     of HEK293T, HELA, MCF7, iBMDM, our experiment can process successfully.
+
                     of HEK293T, HELA, MCF7, iBMDM, so that our experiment can process successfully.
 
                 </p>
 
                 </p>
−
                 <p> Thanks to Dr. <b>Feng Shao</b>’s laboratory, for sharing the cell line of GSDMD<i>-/-</i> HELA cells, it
+
                 <p> Thanks to Dr. <b>Feng Shao</b>’s laboratory, for sharing the cell line of GSDMD<i>-/-</i> HELA cells, which
 
                     provides
 
                     provides
 
                     us great support.
 
                     us great support.
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                     <i>Salmonella enterica</i> var. Typhimurium SL1344.
 
                     <i>Salmonella enterica</i> var. Typhimurium SL1344.
 
                 </p>
 
                 </p>
−
                 <p> Thanks to Dr. Chenli Liu from SIAT CSynBER’s generous share (Strain: <i>E.coli</i> K-12 MG1655 with
+
                 <p> Thanks to Dr. <b>Chenli Liu</b> from SIAT CSynBER for generously sharing the material (Strain: <i>E.coli</i> K-12 MG1655 with
−
                     mCherry in the genome), our experiment can process successfully.
+
                     mCherry in the genome).
 
                 </p>
 
                 </p>
 
                 <div class="h2">Gene Knock In and Knock Out</div>
 
                 <div class="h2">Gene Knock In and Knock Out</div>
 
                 <p>Thanks to Prof. <b>Shan Li</b>’s laboratory, Huazhong Agricultural University, for providing the
 
                 <p>Thanks to Prof. <b>Shan Li</b>’s laboratory, Huazhong Agricultural University, for providing the
 
                     protocol
 
                     protocol
−
                     and material of gene editing based on two-step allelic exchange, it provides us great help.
+
                     and material of gene editing based on two-step allelic exchange, which provides us with great help.
−
                </p>
+
−
                <p> Thanks to Pan Chu, team leader of iGEM-2016, Huazhong Agricultural University, for teaching us
+
−
                    multigene editing based on CRISPR and λ-RED.
+
 
                 </p>
 
                 </p>
 +
               
 
                 <div class="h2">Plasmid Backbone</div>
 
                 <div class="h2">Plasmid Backbone</div>
 
                 <p>Thanks to Prof. <b>Shan Li</b>’s laboratory, Huazhong Agricultural University, for sharing the
 
                 <p>Thanks to Prof. <b>Shan Li</b>’s laboratory, Huazhong Agricultural University, for sharing the
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                     backbone (Pcs2-eGFP) to us.</p>
 
                     backbone (Pcs2-eGFP) to us.</p>
 
                 <div class="h2">Sponsors</div>
 
                 <div class="h2">Sponsors</div>
−
                 <p>Thanks to <b>GeneScript</b> and <b>IDT</b> for DNA sequence synthesis service</p>
+
                 <p>Thanks to <b>GeneScript</b> and <b>IDT</b> for DNA sequence synthesis service.</p>
−
                 <p>Thanks to <b>SnapGene</b> and <b>MathWorks</b> for free software supporting</p>
+
                 <p>Thanks to <b>SnapGene</b> and <b>MathWorks</b> for free software supporting.</p>
 
                 <div class="row">
 
                 <div class="row">
 
                     <div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
 
                     <div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
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                 <br>
 
                 <br>
 
                 <br>
 
                 <br>
−
                 <p>Thanks to College of Life Science and technology of Huazhong Agricultural University for providing
+
                 <p>Thanks to <b>College of Life Science and Technology and Huazhong Agricultural University</b> for providing
−
                     the site and fund for us.
+
                     the working site and fund for us.
 
                 </p>
 
                 </p>
 
                 <div style="width: 50%; margin: 10px auto">
 
                 <div style="width: 50%; margin: 10px auto">
 
                     <img src="https://static.igem.org/mediawiki/2018/1/1e/T--HZAU-China--huanong.png" width=100% alt="">
 
                     <img src="https://static.igem.org/mediawiki/2018/1/1e/T--HZAU-China--huanong.png" width=100% alt="">
 
                 </div>
 
                 </div>
−
                 <p> Thanks to Prof. <b>Binguang Ma</b> for instructing our modeling.
+
                 <p> Thanks to Prof. <b>Binguang Ma</b> as our Primary PI and for supervising the whole project and instructing our modeling.
 
                 </p>
 
                 </p>
 
                 <p> Thanks to Prof. <b>Jin He</b> as our Secondary PI and Prof. <b>Shan Li</b> for instructing our
 
                 <p> Thanks to Prof. <b>Jin He</b> as our Secondary PI and Prof. <b>Shan Li</b> for instructing our
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                     for giving us advices.
 
                     for giving us advices.
 
                 </p>
 
                 </p>
 +
                <p> Thanks to <b>Weitong Guo</b> from iGEM-2017, Huazhong Agricultural University, for the training of
 +
                    new dry-labers during winter vacation.
 
                 <p style="margin-bottom:30px"> We really appreciate your support!
 
                 <p style="margin-bottom:30px"> We really appreciate your support!
 
                 </p>
 
                 </p>

Latest revision as of 02:00, 18 October 2018

Attributions

Our project was designed by Zhujun Xia and all circuits were designed by Zhujun Xia, YinQing Zeng and Mo Qiqin.

Wet Lab
Gene Editing

sifA was knocked out by Zhujun Xia, Zhuoqi Huang and Wenxin Hu also gave assistance.

Plasmid Construction

Intracellular Environment-Dependent Circuits:

The intracellular environment-dependent circuits were designed by Mo Qiqin and Zhujun Xia.

The intracellular environment-dependent circuits were constructed by Mo Qiqin. Ruonan Tian also gave assistance.


Chemical Control System:

The ATc induction chemical control system was constructed by He Yang and Zhuoqi Huang also gave assistance.

The TetR-eGFP fusion protein expression system for TetR expression measurement was constructed by He Yang and Zhuoqi Huang also gave assistance.


Targeting Specificity and Surface-Displaying:

RGD-inserted OmpA circuit clone and construction of Salmonella SL1344_RS05255 (OmpA coding sequence) were completed by YinQing Zeng and Lingyu Zhong.

The circuit of surface-display system was constructed by Lingyu Zhong. Optimation of OmpA in E. Coli was completed by Zhujun Xia.


Plasmid Standardization:

Plasmid standardization was completed by Zhiqing Guo, Hangxi Fu and Zhuoqi Huang by using 3A Assembly.


Plasmid Submission:

Submitted plasmids (Pcs2-eGFP-GSDMD FL, Pcs2-eGFP-GSDMD-N275 and two GSDMD full length mutations) were constructed by Zhiqing Guo and Wenxin Hu.

The description of submitted plasmids by ATP assay and cell phenotype via transfection was designed and completed by Zhujun Xia.

Bacterial Phenotype Verification

Bacterial phenotype verifications were designed and completed by He Yang, including the OD measurement and CFU measurement of chemical control system.

Cell Phenotype Verification

Cell phenotype verifications were designed and completed by Zhujun Xia, including the verification of chemical control system, and targeting specificity, all the related circuits and the demonstration of the final work system.

Biosafety Verification

Safety verification was designed and completed by Zhujun Xia, which demonstrated our project is safe enough to be used.

InterLab

InterLab was conducted by Xichen Rao, Zhujun Xia, Ruonan Tian and Heng Heng also gave assistance. Our data passed the check of the Measurement Committee successfully.

Dry Lab

Mathematical model and app about salmonella infection and GSDMD release were built by Songtao Chen.

ATc model which predicted the relationship between concentration of ATc and concentration of GSDMD was built by Xichen Rao.

The modeling on adjusting the strength of RBS and ATc with fixed GSDMD-N concentration was completed by Yini Miao.

The modeling of the promoter sifA and promoter ent was completed by Ruonan Tian.

Software

The applet of "Bacterial Colony Counter" was designed and programmed by Shuguang Wang.

Human Practice

Collaboration and communication with other teams were completed by Yinqing Zeng and all the other team members.

Handicraft manufacture was designed and completed by Yinqing Zeng.

Questionnaire preparation, the advertisement of synthetic biology and preparation of popular science articles were completed by Heng Heng.

Two posters for Eurasian exchange meetings and CCIC were completed by Ruonan Tian.

Website

Our wiki framework was designed and programmed by Shuguang Wang and our artist were Yuying Xiang and Kening Chen.

Our blog was built by Shuguang Wang.

Others

The plate reader machine operation was conducted by Mo Qiqin.

Chief cleaner of the laboratory was Xichen Rao.

Acknowledgements

For each parts of experiments, our appreciations are as follow:

Cell lines

Thanks to Prof. Shan Li’s laboratory, Huazhong Agricultural University, for sharing the cell lines of HEK293T, HELA, MCF7, iBMDM, so that our experiment can process successfully.

Thanks to Dr. Feng Shao’s laboratory, for sharing the cell line of GSDMD-/- HELA cells, which provides us great support.

Thanks to Dr. Yi Zeng and Dr. Ms Hong Fan, Shanghai Institute of Biochemistry and Cell Biology, CAS, for sharing the cell line of MDA-MB-231.

Bacteria

Thanks to Prof. Shan Li’s laboratory, Huazhong Agricultural University, for sharing the strain of Salmonella enterica var. Typhimurium SL1344.

Thanks to Dr. Chenli Liu from SIAT CSynBER for generously sharing the material (Strain: E.coli K-12 MG1655 with mCherry in the genome).

Gene Knock In and Knock Out

Thanks to Prof. Shan Li’s laboratory, Huazhong Agricultural University, for providing the protocol and material of gene editing based on two-step allelic exchange, which provides us with great help.

Plasmid Backbone

Thanks to Prof. Shan Li’s laboratory, Huazhong Agricultural University, for sharing the plasmid backbone (Pcs2-eGFP) to us.

Sponsors

Thanks to GeneScript and IDT for DNA sequence synthesis service.

Thanks to SnapGene and MathWorks for free software supporting.




Thanks to College of Life Science and Technology and Huazhong Agricultural University for providing the working site and fund for us.

Thanks to Prof. Binguang Ma as our Primary PI and for supervising the whole project and instructing our modeling.

Thanks to Prof. Jin He as our Secondary PI and Prof. Shan Li for instructing our experiment.

Thanks to Prof. Jin He, Prof. Shan Li, Prof. Gang Cao, Prof. Donghai Peng and Prof. Ming Sun for giving us advices at the proposal presentation.

Thanks to Kening Chen, Wenqi Huang and Haimeng Li from iGEM-2017, Huazhong Agricultural University, for giving us advices.

Thanks to Weitong Guo from iGEM-2017, Huazhong Agricultural University, for the training of new dry-labers during winter vacation.

We really appreciate your support!

Team
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