Difference between revisions of "Team:Warwick"

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<h3>Project Description</h3>
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<p>The theme of our iGEM entry this year is water safety. In particular, we will focus on using synthetic biology to achieve two goals. The first of these goals is to develop a novel method for detecting Legionella spp. in water systems, with the ability to distinguish between live and dead bacteria. Legionella is the bacteria that is responsible for causing Legionnaire’s disease in humans – a disease that causes pneumonia. It lives only in aquatic environments, usually as an intracellular parasite of protozoa such as amoebas. Legionnaire’s disease currently has no vaccine, so prevention relies on maintenance of Legionella spp. free water supplies. Currently we are looking at detecting Legionella mRNA using a complementary guide RNA that activates dCas9 when binding, to express GFP on a plasmid.
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<p>Our second goal is to develop and sequence a strain of E. coli that can utilise oestrogen as a source of carbon. Modern practises of society including agriculture and pharmaceuticals have increased the level of oestrogen in natural water sources such as rivers and lakes. This poses a large risk to aquatic species, especially fish, and has been shown to cause collapse of fish populations. We aim to investigate genetically modified bacteria as a potential starting point for a solution to this problem. By using directed evolution, we aim to synthesise a strain of E. coli that has the ability to use oestrogen as a carbon source, and then use sequencing to observe and study the genetic changes that allow it to do so.
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<span id='WDT0'>Biological</span>
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    <br><br><br><br><br>
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    <span id='WDT1'>Organic</span>
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    <span id='WDT2'>Inorganic</span>
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    <br><br><br><br><br>
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    <span id='WDT3'>Solutions to clean water</span>
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<div class='contentDivs' id='CDiv1'>
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<h1>Why water safety?</h1>
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<p>Access to safe water is among the many crises we face as a growing population. The WHO estimates that 2.1 billion people lack access to safely managed drinking water services (http://www.un.org/en/sections/issues-depth/water/) , and it is estimated that $260B lost globally each year due to lack of basic water. <a href='https://water.org/our-impact/water-crisis/'>source</a><br><br>When we tried to break down this problem, we were overwhelmed by the many different types of contaminants in water, each of which require different approaches to their removal. We decided to look into how to remove on of each in three main sub-categories of contaminants: Biological, Organic and Inorganic.<br><br>Looking into this event, we realised there were many different types of contaminants in water, each of which require different approaches to their removal. We decided to look into how to remove on of each in three main sub-categories of contaminants: Biological, Organic and Inorganic.</p>
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<h1>Three very different problems: Lead, Legionella and Oestrogen.</h1>
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<p>We are tackling the bioremediation of waters, focusing on three water pollutants in particular: the organic (oestrogen), inorganic (lead) and biological (legionella). Core concepts of synthetic biology were used create circuits which degrade, remove and detect these pollutants respectively.<br><br>Our projects are ambitious and attempt to solve a wide range of problems that aren’t connected to each other by a unified solution, and that’s not what we are trying to come up with. Each of these areas we have invested in were chosen based on the resources we had as a university in terms of expertise, equipment and experience.</p>
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<span style='font-weight: 400; color: white; line-height: normal;'>Making water <span style='font-weight: 700;'>safe</span> again,</span>
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<span style='font-weight: 300; color: white;'> one step at a time</span>
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</h1><br>
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Here on the Ripple iGEM team we are dedicating ourselves to making water safe again; not just for the ourselves, but for the surrounding environments and wildlife too.
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</p>
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<br>
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Latest revision as of 02:10, 18 October 2018

Ripple

Ripple




Biological




Organic




Inorganic




Solutions to clean water

Why water safety?

Access to safe water is among the many crises we face as a growing population. The WHO estimates that 2.1 billion people lack access to safely managed drinking water services (http://www.un.org/en/sections/issues-depth/water/) , and it is estimated that $260B lost globally each year due to lack of basic water. source

When we tried to break down this problem, we were overwhelmed by the many different types of contaminants in water, each of which require different approaches to their removal. We decided to look into how to remove on of each in three main sub-categories of contaminants: Biological, Organic and Inorganic.

Looking into this event, we realised there were many different types of contaminants in water, each of which require different approaches to their removal. We decided to look into how to remove on of each in three main sub-categories of contaminants: Biological, Organic and Inorganic.

Three very different problems: Lead, Legionella and Oestrogen.

We are tackling the bioremediation of waters, focusing on three water pollutants in particular: the organic (oestrogen), inorganic (lead) and biological (legionella). Core concepts of synthetic biology were used create circuits which degrade, remove and detect these pollutants respectively.

Our projects are ambitious and attempt to solve a wide range of problems that aren’t connected to each other by a unified solution, and that’s not what we are trying to come up with. Each of these areas we have invested in were chosen based on the resources we had as a university in terms of expertise, equipment and experience.

Click one

Ripple is

Making water safe again, one step at a time


Here on the Ripple iGEM team we are dedicating ourselves to making water safe again; not just for the ourselves, but for the surrounding environments and wildlife too.