Difference between revisions of "Team:Purdue/Parts"

 
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<h1>Parts</h1>
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<div class='title'>Parts</div>
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<h3>Note</h3>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<th>Part Name</th>
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<th>Description</th>
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<th>Type</th>
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<th>Function</th>
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<th style="width:120px;">Length (bp)</th>
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<td><a href="http://parts.igem.org/Part:BBa_K2696000">BBa_K2696000</a></td>
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<td>Gal 1 Promoter</td>
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<td>Regulatory</td>
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<td>Galactose inducible promoter. Curran, Kathleen A., et al. “Short Synthetic Terminators for Improved Heterologous Gene Expression in Yeast.” ACS Synthetic Biology, vol. 4, no. 7, 2015, pp. 824–832., doi:10.1021/sb5003357.</td>
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<td>442</td>
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<h3>Adding parts to the registry</h3>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<td><a href="http://parts.igem.org/Part:BBa_K2696001">BBa_K2696001</a></td>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<td>Full Length Horseradish Peroxidase (HRP-FL)</td>
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<td>Coding</td>
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<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
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<td>A peroxidase commonly used in ELISA and related assays for its ability to catalyze reactions which convert colorless substrates like tetramethylbenzidine (TMB) to colored products. Martell, Jeffrey D, et al. “A Split Horseradish Peroxidase for the Detection of Intercellular Protein–Protein Interactions and Sensitive Visualization of Synapses.” Nature Biotechnology, vol. 34, no. 7, 2016, pp. 774–780., doi:10.1038/nbt.3563.</td>
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ADD PARTS
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<td>927</td>
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<td><a href="http://parts.igem.org/Part:BBa_K2696002">BBa_K2696002</a></td>
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</div>
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<td>Terminator 8 </td>
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<td>Terminator</td>
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<td>A eukaryotic terminator derived from a native terminator in Saccharomyces cerevisiae. Curran, Kathleen A., et al. “Short Synthetic Terminators for Improved Heterologous Gene Expression in Yeast.” ACS Synthetic Biology, vol. 4, no. 7, 2015, pp. 824–832., doi:10.1021/sb5003357.</td>
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<td>49</td>
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<td><a href="http://parts.igem.org/Part:BBa_K2696003">BBa_K2696003</a></td>
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<h3>Inspiration</h3>
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<td>HRP Yeast Operon</td>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<td>Composite</td>
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<td>Composed of a Gal1 Promoter (Induced by galactose in absence of other energy sources), Horseradish peroxidase, and a synthetic terminator originally derived from S. cerevisiae.</td>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<td>1434</td>
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<ul>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<td><a href="http://parts.igem.org/Part:BBa_K2696004">BBa_K2696004</a></td>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<td>Gyp 7 Terminator</td>
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</ul>
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<td>Terminator</td>
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<td>Terminator derived from the gyp 7 gene.</td>
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</div>
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<td>112</td>
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<div class="clear extra_space"></div>
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<td><a href="http://parts.igem.org/Part:BBa_K2696005">BBa_K2696005</a></td>
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<td>His 5 Promoter</td>
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<td>Promoter</td>
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<td>Constitutive promoter</td>
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<td>500</td>
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<h3>What information do I need to start putting my parts on the Registry?</h3>
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<td><a href="http://parts.igem.org/Part:BBa_K2696006">BBa_K2696006</a></td>
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<p>The information needed to initially create a part on the Registry is:</p>
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<td>HRP (59-308) </td>
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<ul>
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<td>Coding</td>
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<li>Part Name</li>
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<td>Codes for the later half of horseradish peroxidase from amino acids 59 through 308. Selected for its ability to reconstitute at room temperature. Martell, Jeffrey D, et al. “A Split Horseradish Peroxidase for the Detection of Intercellular Protein–Protein Interactions and Sensitive Visualization of Synapses.” Nature Biotechnology, vol. 34, no. 7, 2016, pp. 774–780., doi:10.1038/nbt.3563.</td>
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<li>Part type</li>
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<td>753</td>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<td><a href="http://parts.igem.org/Part:BBa_K2696007">BBa_K2696007</a></td>
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<li>Long Description (Longer description of what the DNA does)</li>
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<td>HRP (start + 1-58)</td>
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<li>Design considerations</li>
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<td>Coding</td>
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</ul>
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<td>Codes for the first half of horseradish peroxidase from amino acids 1 through 58. It also includes a start codon. Selected for its ability to reconstitute at room temperature.  Martell, Jeffrey D, et al. “A Split Horseradish Peroxidase for the Detection of Intercellular Protein–Protein Interactions and Sensitive Visualization of Synapses.” Nature Biotechnology, vol. 34, no. 7, 2016, pp. 774–780., doi:10.1038/nbt.3563.</td>
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<td>117</td>
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<p>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<td><a href="http://parts.igem.org/Part:BBa_K2696008">BBa_K2696008</a></td>
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</div>
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<td>Igk-flag-FRP-HRP(1-58)</td>
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<td>Coding</td>
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<td>First part of the locking mechanism. "Igk-flag" represents the tag on the protein. Martell, Jeffrey D, et al. “A Split Horseradish Peroxidase for the Detection of Intercellular Protein–Protein Interactions and Sensitive Visualization of Synapses.” Nature Biotechnology, vol. 34, no. 7, 2016, pp. 774–780., doi:10.1038/nbt.3563.</td>
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<td>585</td>
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<td><a href="http://parts.igem.org/Part:BBa_K2696009">BBa_K2696009</a></td>
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<td>Igk-myc-FKB12-HRP(59-308)</td>
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<h3>Part Table </h3>
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<td>Coding</td>
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<td>Second part of locking mechanism. “Igk-myc” represents the tag on the protein.  Martell, Jeffrey D, et al. “A Split Horseradish Peroxidase for the Detection of Intercellular Protein–Protein Interactions and Sensitive Visualization of Synapses.” Nature Biotechnology, vol. 34, no. 7, 2016, pp. 774–780., doi:10.1038/nbt.3563.</td>
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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<td>1209</td>
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<groupparts>iGEM18 Purdue</groupparts>
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<td><a href="http://parts.igem.org/Part:BBa_K2696010">BBa_K2696010</a></td>
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<td>pqsR</td>
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<td>Coding</td>
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<td>pqsR (<i>Pseudomonas quinolone</i> signal Receptor) is a protein capable of binding to farnesol.</td>
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<td>999</td>
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<td><a href="http://parts.igem.org/Part:BBa_K2696011">BBa_K2696011</a></td>
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<td>Terminator 6</td>
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<td>Terminator</td>
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<td>A eukaryotic terminator derived from a native terminator in Saccharomyces cerevisiae. Curran, Kathleen A., et al. “Short Synthetic Terminators for Improved Heterologous Gene Expression in Yeast.” ACS Synthetic Biology, vol. 4, no. 7, 2015, pp. 824–832., doi:10.1021/sb5003357.</td>
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<td>32</td>
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</tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2696012">BBa_K2696012</a></td>
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<td>TetO-Cyc1 promoter (Tet-Off)</td>
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<td>Regulatory</td>
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<td>When tetracycline is present, it inhibits the transcription of the gene regulated by this promoter.</td>
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<td>160</td>
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</tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2696013">BBa_K2696013</a></td>
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<td>TetR-VP16</td>
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<td>Signaling</td>
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<td>TetR receptor fused with the C-domain of VP16 (viral protein 16), which is a transcriptional activation domain from HSV (herpes simplex virus).</td>
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<td>1008</td>
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</tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2696014">BBa_K2696014</a></td>
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<td>Tyrosinase</td>
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<td>Coding</td>
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<td>Tyrosinase is a protein capable of binding tyrosol.</td>
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<td>1836</td>
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{{:Team:Purdue/Footer}}

Latest revision as of 02:21, 18 October 2018

Bootstrap Example

Parts
Part Name Description Type Function Length (bp)
BBa_K2696000 Gal 1 Promoter Regulatory Galactose inducible promoter. Curran, Kathleen A., et al. “Short Synthetic Terminators for Improved Heterologous Gene Expression in Yeast.” ACS Synthetic Biology, vol. 4, no. 7, 2015, pp. 824–832., doi:10.1021/sb5003357. 442
BBa_K2696001 Full Length Horseradish Peroxidase (HRP-FL) Coding A peroxidase commonly used in ELISA and related assays for its ability to catalyze reactions which convert colorless substrates like tetramethylbenzidine (TMB) to colored products. Martell, Jeffrey D, et al. “A Split Horseradish Peroxidase for the Detection of Intercellular Protein–Protein Interactions and Sensitive Visualization of Synapses.” Nature Biotechnology, vol. 34, no. 7, 2016, pp. 774–780., doi:10.1038/nbt.3563. 927
BBa_K2696002 Terminator 8 Terminator A eukaryotic terminator derived from a native terminator in Saccharomyces cerevisiae. Curran, Kathleen A., et al. “Short Synthetic Terminators for Improved Heterologous Gene Expression in Yeast.” ACS Synthetic Biology, vol. 4, no. 7, 2015, pp. 824–832., doi:10.1021/sb5003357. 49
BBa_K2696003 HRP Yeast Operon Composite Composed of a Gal1 Promoter (Induced by galactose in absence of other energy sources), Horseradish peroxidase, and a synthetic terminator originally derived from S. cerevisiae. 1434
BBa_K2696004 Gyp 7 Terminator Terminator Terminator derived from the gyp 7 gene. 112
BBa_K2696005 His 5 Promoter Promoter Constitutive promoter 500
BBa_K2696006 HRP (59-308) Coding Codes for the later half of horseradish peroxidase from amino acids 59 through 308. Selected for its ability to reconstitute at room temperature. Martell, Jeffrey D, et al. “A Split Horseradish Peroxidase for the Detection of Intercellular Protein–Protein Interactions and Sensitive Visualization of Synapses.” Nature Biotechnology, vol. 34, no. 7, 2016, pp. 774–780., doi:10.1038/nbt.3563. 753
BBa_K2696007 HRP (start + 1-58) Coding Codes for the first half of horseradish peroxidase from amino acids 1 through 58. It also includes a start codon. Selected for its ability to reconstitute at room temperature. Martell, Jeffrey D, et al. “A Split Horseradish Peroxidase for the Detection of Intercellular Protein–Protein Interactions and Sensitive Visualization of Synapses.” Nature Biotechnology, vol. 34, no. 7, 2016, pp. 774–780., doi:10.1038/nbt.3563. 117
BBa_K2696008 Igk-flag-FRP-HRP(1-58) Coding First part of the locking mechanism. "Igk-flag" represents the tag on the protein. Martell, Jeffrey D, et al. “A Split Horseradish Peroxidase for the Detection of Intercellular Protein–Protein Interactions and Sensitive Visualization of Synapses.” Nature Biotechnology, vol. 34, no. 7, 2016, pp. 774–780., doi:10.1038/nbt.3563. 585
BBa_K2696009 Igk-myc-FKB12-HRP(59-308) Coding Second part of locking mechanism. “Igk-myc” represents the tag on the protein. Martell, Jeffrey D, et al. “A Split Horseradish Peroxidase for the Detection of Intercellular Protein–Protein Interactions and Sensitive Visualization of Synapses.” Nature Biotechnology, vol. 34, no. 7, 2016, pp. 774–780., doi:10.1038/nbt.3563. 1209
BBa_K2696010 pqsR Coding pqsR (Pseudomonas quinolone signal Receptor) is a protein capable of binding to farnesol. 999
BBa_K2696011 Terminator 6 Terminator A eukaryotic terminator derived from a native terminator in Saccharomyces cerevisiae. Curran, Kathleen A., et al. “Short Synthetic Terminators for Improved Heterologous Gene Expression in Yeast.” ACS Synthetic Biology, vol. 4, no. 7, 2015, pp. 824–832., doi:10.1021/sb5003357. 32
BBa_K2696012 TetO-Cyc1 promoter (Tet-Off) Regulatory When tetracycline is present, it inhibits the transcription of the gene regulated by this promoter. 160
BBa_K2696013 TetR-VP16 Signaling TetR receptor fused with the C-domain of VP16 (viral protein 16), which is a transcriptional activation domain from HSV (herpes simplex virus). 1008
BBa_K2696014 Tyrosinase Coding Tyrosinase is a protein capable of binding tyrosol. 1836

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