Difference between revisions of "Team:Purdue/Parts"
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<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2696000</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K2696000">BBa_K2696000</a></td> |
<td>Gal 1 Promoter</td> | <td>Gal 1 Promoter</td> | ||
<td>Regulatory</td> | <td>Regulatory</td> | ||
| Line 29: | Line 29: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2696001</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K2696001">BBa_K2696001</a></td> |
<td>Full Length Horseradish Peroxidase (HRP-FL)</td> | <td>Full Length Horseradish Peroxidase (HRP-FL)</td> | ||
<td>Coding</td> | <td>Coding</td> | ||
| Line 36: | Line 36: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2696002</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K2696002">BBa_K2696002</a></td> |
<td>Terminator 8 </td> | <td>Terminator 8 </td> | ||
<td>Terminator</td> | <td>Terminator</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2696003</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K2696003">BBa_K2696003</a></td> |
<td>HRP Yeast Operon</td> | <td>HRP Yeast Operon</td> | ||
<td>Composite</td> | <td>Composite</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2696004</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K2696004">BBa_K2696004</a></td> |
<td>Gyp 7 Terminator</td> | <td>Gyp 7 Terminator</td> | ||
<td>Terminator</td> | <td>Terminator</td> | ||
| Line 57: | Line 57: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2696005</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K2696005">BBa_K2696005</a></td> |
<td>His 5 Promoter</td> | <td>His 5 Promoter</td> | ||
<td>Promoter</td> | <td>Promoter</td> | ||
| Line 64: | Line 64: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2696006</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K2696006">BBa_K2696006</a></td> |
<td>HRP (59-308) </td> | <td>HRP (59-308) </td> | ||
<td>Coding</td> | <td>Coding</td> | ||
| Line 71: | Line 71: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2696007</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K2696007">BBa_K2696007</a></td> |
<td>HRP (start + 1-58)</td> | <td>HRP (start + 1-58)</td> | ||
<td>Coding</td> | <td>Coding</td> | ||
| Line 78: | Line 78: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2696008</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K2696008">BBa_K2696008</a></td> |
<td>Igk-flag-FRP-HRP(1-58)</td> | <td>Igk-flag-FRP-HRP(1-58)</td> | ||
<td>Coding</td> | <td>Coding</td> | ||
| Line 85: | Line 85: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2696009</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K2696009">BBa_K2696009</a></td> |
<td>Igk-myc-FKB12-HRP(59-308)</td> | <td>Igk-myc-FKB12-HRP(59-308)</td> | ||
<td>Coding</td> | <td>Coding</td> | ||
| Line 91: | Line 91: | ||
<td>1209</td> | <td>1209</td> | ||
</tr> | </tr> | ||
| + | |||
<tr> | <tr> | ||
| − | <td> | + | <td><a href="http://parts.igem.org/Part:BBa_K2696010">BBa_K2696010</a></td> |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
<td>pqsR</td> | <td>pqsR</td> | ||
<td>Coding</td> | <td>Coding</td> | ||
| Line 106: | Line 100: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2696011</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K2696011">BBa_K2696011</a></td> |
<td>Terminator 6</td> | <td>Terminator 6</td> | ||
<td>Terminator</td> | <td>Terminator</td> | ||
| − | <td> | + | <td>A eukaryotic terminator derived from a native terminator in Saccharomyces cerevisiae. Curran, Kathleen A., et al. “Short Synthetic Terminators for Improved Heterologous Gene Expression in Yeast.” ACS Synthetic Biology, vol. 4, no. 7, 2015, pp. 824–832., doi:10.1021/sb5003357.</td> |
<td>32</td> | <td>32</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2696012</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K2696012">BBa_K2696012</a></td> |
<td>TetO-Cyc1 promoter (Tet-Off)</td> | <td>TetO-Cyc1 promoter (Tet-Off)</td> | ||
<td>Regulatory</td> | <td>Regulatory</td> | ||
| Line 120: | Line 114: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2696013</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K2696013">BBa_K2696013</a></td> |
<td>TetR-VP16</td> | <td>TetR-VP16</td> | ||
<td>Signaling</td> | <td>Signaling</td> | ||
| − | <td>TetR-VP16 | + | <td>TetR receptor fused with the C-domain of VP16 (viral protein 16), which is a transcriptional activation domain from HSV (herpes simplex virus).</td> |
<td>1008</td> | <td>1008</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2696014</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K2696014">BBa_K2696014</a></td> |
<td>Tyrosinase</td> | <td>Tyrosinase</td> | ||
<td>Coding</td> | <td>Coding</td> | ||
| Line 138: | Line 132: | ||
</div> | </div> | ||
</div> | </div> | ||
| − | |||
</body> | </body> | ||
</html> | </html> | ||
{{:Team:Purdue/Footer}} | {{:Team:Purdue/Footer}} | ||
Latest revision as of 02:21, 18 October 2018
Parts
| Part Name | Description | Type | Function | Length (bp) |
|---|---|---|---|---|
| BBa_K2696000 | Gal 1 Promoter | Regulatory | Galactose inducible promoter. Curran, Kathleen A., et al. “Short Synthetic Terminators for Improved Heterologous Gene Expression in Yeast.” ACS Synthetic Biology, vol. 4, no. 7, 2015, pp. 824–832., doi:10.1021/sb5003357. | 442 |
| BBa_K2696001 | Full Length Horseradish Peroxidase (HRP-FL) | Coding | A peroxidase commonly used in ELISA and related assays for its ability to catalyze reactions which convert colorless substrates like tetramethylbenzidine (TMB) to colored products. Martell, Jeffrey D, et al. “A Split Horseradish Peroxidase for the Detection of Intercellular Protein–Protein Interactions and Sensitive Visualization of Synapses.” Nature Biotechnology, vol. 34, no. 7, 2016, pp. 774–780., doi:10.1038/nbt.3563. | 927 |
| BBa_K2696002 | Terminator 8 | Terminator | A eukaryotic terminator derived from a native terminator in Saccharomyces cerevisiae. Curran, Kathleen A., et al. “Short Synthetic Terminators for Improved Heterologous Gene Expression in Yeast.” ACS Synthetic Biology, vol. 4, no. 7, 2015, pp. 824–832., doi:10.1021/sb5003357. | 49 |
| BBa_K2696003 | HRP Yeast Operon | Composite | Composed of a Gal1 Promoter (Induced by galactose in absence of other energy sources), Horseradish peroxidase, and a synthetic terminator originally derived from S. cerevisiae. | 1434 |
| BBa_K2696004 | Gyp 7 Terminator | Terminator | Terminator derived from the gyp 7 gene. | 112 |
| BBa_K2696005 | His 5 Promoter | Promoter | Constitutive promoter | 500 |
| BBa_K2696006 | HRP (59-308) | Coding | Codes for the later half of horseradish peroxidase from amino acids 59 through 308. Selected for its ability to reconstitute at room temperature. Martell, Jeffrey D, et al. “A Split Horseradish Peroxidase for the Detection of Intercellular Protein–Protein Interactions and Sensitive Visualization of Synapses.” Nature Biotechnology, vol. 34, no. 7, 2016, pp. 774–780., doi:10.1038/nbt.3563. | 753 |
| BBa_K2696007 | HRP (start + 1-58) | Coding | Codes for the first half of horseradish peroxidase from amino acids 1 through 58. It also includes a start codon. Selected for its ability to reconstitute at room temperature. Martell, Jeffrey D, et al. “A Split Horseradish Peroxidase for the Detection of Intercellular Protein–Protein Interactions and Sensitive Visualization of Synapses.” Nature Biotechnology, vol. 34, no. 7, 2016, pp. 774–780., doi:10.1038/nbt.3563. | 117 |
| BBa_K2696008 | Igk-flag-FRP-HRP(1-58) | Coding | First part of the locking mechanism. "Igk-flag" represents the tag on the protein. Martell, Jeffrey D, et al. “A Split Horseradish Peroxidase for the Detection of Intercellular Protein–Protein Interactions and Sensitive Visualization of Synapses.” Nature Biotechnology, vol. 34, no. 7, 2016, pp. 774–780., doi:10.1038/nbt.3563. | 585 |
| BBa_K2696009 | Igk-myc-FKB12-HRP(59-308) | Coding | Second part of locking mechanism. “Igk-myc” represents the tag on the protein. Martell, Jeffrey D, et al. “A Split Horseradish Peroxidase for the Detection of Intercellular Protein–Protein Interactions and Sensitive Visualization of Synapses.” Nature Biotechnology, vol. 34, no. 7, 2016, pp. 774–780., doi:10.1038/nbt.3563. | 1209 |
| BBa_K2696010 | pqsR | Coding | pqsR (Pseudomonas quinolone signal Receptor) is a protein capable of binding to farnesol. | 999 |
| BBa_K2696011 | Terminator 6 | Terminator | A eukaryotic terminator derived from a native terminator in Saccharomyces cerevisiae. Curran, Kathleen A., et al. “Short Synthetic Terminators for Improved Heterologous Gene Expression in Yeast.” ACS Synthetic Biology, vol. 4, no. 7, 2015, pp. 824–832., doi:10.1021/sb5003357. | 32 |
| BBa_K2696012 | TetO-Cyc1 promoter (Tet-Off) | Regulatory | When tetracycline is present, it inhibits the transcription of the gene regulated by this promoter. | 160 |
| BBa_K2696013 | TetR-VP16 | Signaling | TetR receptor fused with the C-domain of VP16 (viral protein 16), which is a transcriptional activation domain from HSV (herpes simplex virus). | 1008 |
| BBa_K2696014 | Tyrosinase | Coding | Tyrosinase is a protein capable of binding tyrosol. | 1836 |
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