Difference between revisions of "Team:East Chapel Hill"

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{{East_Chapel_Hill}}
 
{{East_Chapel_Hill}}
 
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<title>Home</title>
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    <!-- Bootstrap -->
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<link href="https://2018.igem.org/Template:East_Chapel_Hill rel="stylesheet">
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/*h2 {
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<h1> Welcome to iGEM 2018! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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font-weight: 300;*/
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<h3>Before you start</h3>
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<!-- CONTENT BEGINS HERE -->
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<p> Please read the following pages:</p>
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<body>
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<ul>
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<li>  <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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<li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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<h3> Styling your wiki </h3>
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        <img src="https://static.igem.org/mediawiki/2018/9/90/T--East_Chapel_Hill--slide2.png"  style="width:100%;">
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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<p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
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<h1><p2>Improving the Efficacy of Riboswitch Based Sensor for Visual Detection of Excess Fluoride in Water</h1></p2>
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<p2> Scientists around the world have recognized fluoride as a markedly beneficial resource for protecting tooth enamel from decay. However, excess fluoride can also have an adverse effect on human health. A significant problem arises when excess amounts of fluoride infiltrate drinking water. High fluoride concentrations can have devastating impacts on dental health, especially those of children, and eventually lead to hypomineralization and dental fluorosis.
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<br></br>
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To address this challenge, we aim to first develop an efficient, user-friendly, and cost-sensitive fluoride biosensor using previously characterized fluoride riboswitches. Last year, we developed an operon whose riboswitch was only activated when bound to fluoride. This fluoride-specific activation allowed for selective transcription of the chloramphenicol acetyltransferase (CAT) gene. Bacteria that can express the CAT gene have a resistance to the chloramphenicol antibiotic, and can survive in the presence of chloramphenicol. Consequently, the presence of fluoride allows for bacterial growth in the presence of chloramphenicol. However, this system was not able to detect concentrations low enough to prove useful in realistic applications. This summer, we focused on testing various promoters and riboswitch sequences to reduce the detection threshold and improve the efficacy of our previous operon. 
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<h3> Wiki template information </h3>
 
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
 
  
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<h3> Editing your wiki </h3>
 
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
 
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<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
 
  
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<a href="https://2018.igem.org/wiki/index.php?title=Team:East_Chapel_Hill&action=edit">
 
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EDIT PAGE
 
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
 
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<ul>
 
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
 
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<li>Be clear about what you are doing and how you plan to do this.</li>
 
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
 
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
 
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
 
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
 
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<li>Have lots of fun! </li>
 
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<div class="highlight decoration_A_full">
 
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<h3>Inspiration</h3>
 
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
 
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<ul>
 
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
 
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
 
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
 
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
 
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
 
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
 
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</ul>
 
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</body>
 
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Latest revision as of 02:27, 18 October 2018

East Chapel Hill
Home

Improving the Efficacy of Riboswitch Based Sensor for Visual Detection of Excess Fluoride in Water

Scientists around the world have recognized fluoride as a markedly beneficial resource for protecting tooth enamel from decay. However, excess fluoride can also have an adverse effect on human health. A significant problem arises when excess amounts of fluoride infiltrate drinking water. High fluoride concentrations can have devastating impacts on dental health, especially those of children, and eventually lead to hypomineralization and dental fluorosis.

To address this challenge, we aim to first develop an efficient, user-friendly, and cost-sensitive fluoride biosensor using previously characterized fluoride riboswitches. Last year, we developed an operon whose riboswitch was only activated when bound to fluoride. This fluoride-specific activation allowed for selective transcription of the chloramphenicol acetyltransferase (CAT) gene. Bacteria that can express the CAT gene have a resistance to the chloramphenicol antibiotic, and can survive in the presence of chloramphenicol. Consequently, the presence of fluoride allows for bacterial growth in the presence of chloramphenicol. However, this system was not able to detect concentrations low enough to prove useful in realistic applications. This summer, we focused on testing various promoters and riboswitch sequences to reduce the detection threshold and improve the efficacy of our previous operon.