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| − | <div class="contentbox">
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| − | <h1 class="box-heading">Description</h1>
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| − | <p>We insert fragment of Cecropin AD into vector pET-28a(Figure 1) </p>
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| − | <figure>
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| − | <figure class="makeresponsive floatleft" style="width: 70%;margin-left:15%;margin-right:30%;">
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| − | <img src="https://static.igem.org/mediawiki/2018/b/b2/T--ECUST--BiocideF1.png" class="zoom">
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| − | <figcaption><b>Figure 1. The vector pET-28a Vector is cut by NcoI and BamHI. Sequence of AD was chemically synthesized and amplified by PCR, then ligated with linearized vector by Ezmax.</b></figcaption>
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| − | </figure>
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| − | <h1 class="box-heading">Design</h1>
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| − | <p>The plasmid was transformed to E. coli BL21 and cultured at 37 °C for 12 h. positive monoclonal bacteria were cultured and verified by PCR(Figure 2). </p>
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| − | <figure>
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| − | <figure class="makeresponsive" style="width: 50%;margin-left:23%;margin-right:30%;">
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| − | <img src="https://static.igem.org/mediawiki/2018/4/47/T--ECUST--result--biocide_2.jpg" class="f1">
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| − | <figcaption><b>Figure 2. 1% Agarose Gel Electrophoresis of PCR, which shows that our vector was successfully constructed.</b></figcaption>
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| − | </figure>
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| − | </figure>
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| − | </div>
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| − | <div class="contentbox">
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| − | <h1 class="box-heading">Result</h1>
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| − | <p>We verified the expression of Cecropin AD by SDS-PAGE(Figure 3). </p>
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| − | <figure>
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| − | <figure class="makeresponsive" style="width: 50%;margin-left:42%;margin-right:30%;">
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| − | <img src="https://static.igem.org/mediawiki/2018/4/46/T--ECUST--result--biocide_3.jpg" class="f3">
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| − | <figcaption><b>Figure 3. The SDS-PAGE of Cecropin AD
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| − | Lane 1 :before induction
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| − | Lane 2,3:after induction
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| − | </b></figcaption>
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| − | </figure>
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| − | </figure>
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| − |
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| − | <p>To test the effect of cecropin AD, we measured the growth curve of iron bacteria adding with different concentration of cecropin AD.</p>
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| − | <figure>
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| − | <figure class="makeresponsive" style="width: 50%;margin-left:42%;margin-right:30%;">
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| − | <img src="https://static.igem.org/mediawiki/2018/2/2a/T--ECUST--B4.jpg>
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| − | <figcaption><b>Figure 4. Growth curve of iron bacteria adding with different concentration of cecropin AD
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| − | </b></figcaption>
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| − | </figure>
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| − | </figure>
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| − | <p>The figure shows that cecropin AD has powerful bacterividal activity. Only concentration of cecropin AD is low enough, can iron bacteria grow.</p>
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| − | </div>
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| | </main> | | </main> |
