Difference between revisions of "Team:TecMonterrey GDL/Parts"

 
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        <h1><img class="img-decorator2" alt="decoration2" src="https://static.igem.org/mediawiki/2018/3/3a/T--TecMonterrey_GDL--decoration1.png">Mathematical Model <img class="img-decorator" alt="decoration1" src="https://static.igem.org/mediawiki/2018/3/3a/T--TecMonterrey_GDL--decoration1.png"></h1>
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                    <li><a href="https://2018.igem.org/Team:TecMonterrey_GDL/GeneticE">Genetic engineering</a></li>
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<!--End Main Header -->
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<section class="page-title text-center" style="background-image:url(https://static.igem.org/mediawiki/2018/0/0c/T--TecMonterrey_GDL--Parts--ENCABEZADO.png);">
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    <div class="container">
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        <div class="title-text">
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            <h1>Parts</h1>
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                        <h1 align="center"> <span>Parts</span> </h1>
 
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<h3 class="writing">In the following table we present the parts we have used for our plasmid construction, detailed in Constructs, the parts we used are classified as they appear in our composite constructs:
 
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</h3>
 
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<h4 class="writing"> For E: Coli </h4>
 
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<h3 align="right"> Strategy A:  E. coli
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<section class="service-overview section">
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    <div class="container">
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<img src="https://static.igem.org/mediawiki/2018/f/f1/T--TecMonterrey_GDL--Construct3.png" align="right">
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<h1></h1>
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<h3 align="left"> Simbology </h3>
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<img src="https://static.igem.org/mediawiki/2018/4/48/T--TecMonterrey_GDL--Construct5.png" align="left">
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<h1></h1>
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<h2 style="color:#00008B" align="center" > Table A. Strategy A:  E. coli  </h2>
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<h3 style="color:#00008B" align="center"> (all optimized for E. coli)</h3>
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<img src="https://static.igem.org/mediawiki/2018/c/cb/T--TecMonterrey_GDL--Table1.png">
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<h1></h1>
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          <div class="col-md-12">
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<h4 class="writing"> For E: Coli </h4>
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            <div class="contents">
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<h2 style="color:#00008B" align="right"> Strategy B: E. coli
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                <div class="section-title">
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</h2>
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                    <h3>For <i>E.coli</i></h3>
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<img src="https://static.igem.org/mediawiki/2018/c/c1/T--TecMonterrey_GDL--ConstructReal2.png" align="right">
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                </div>
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<h1></h1>
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                <div class="text">
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<h3 align="left"> Simbology </h3>
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                    <p style="text-align:center; font-size: 18px;">
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<img src="https://static.igem.org/mediawiki/2018/4/48/T--TecMonterrey_GDL--Construct5.png" align="left">
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                      In the following table we present the parts we have used for our plasmid construction, detailed in
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<h1></h1>
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                      <a href="https://2018.igem.org/Team:TecMonterrey_GDL/Composite_Part">Construct design</a>, the parts we used are classified as they appear in our composite constructs:
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<h1></h1>
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                    </p>
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    <div>
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                </div>
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<h2 style="color:#00008B" align="center" > Table B. Strategy B: E. coli </h2>
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              </div>
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<h3 style="color:#00008B" align="center"> (all optimized for E. coli)</h3>
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              <div class="row">
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<img src="https://static.igem.org/mediawiki/2018/2/2d/T--TecMonterrey_GDL--Table2.png">
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                <center>
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                  <br>
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</div>
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                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/b/b6/T--TecMonterrey_GDL--Parts--2.png" alt="service-image">
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                </center>
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              </div>
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              <div class="row">
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                <div class="col-md-6">
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                  <br>
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                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/3/31/T--TecMonterrey_GDL--Parts--Tabla1.png" alt="service-image">
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                </div>
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                <div class="col-md-6">
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                  <br><br><br><br><br><br><br>
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                  <center>
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                  <h4><a href="http://parts.igem.org/Part:BBa_K2760010:Design#Source">Strategy A : Part:BBa_K2760010 - Lactobachill Plan A (E. coli)</a></h4>
 +
                </center><br>
 +
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/c/c2/T--TecMonterrey_GDL--Parts--1.png" alt="service-image">
  
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    <div>
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                  <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K206000">BBa_K206000</a>, <a href="http://parts.igem.org/Part:BBa_J34801">BBa_J34801</a>, <a href="http://parts.igem.org/Part:BBa_K2760023">BBa_K2760023</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>,
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<h3 align="center"> For L. rhamnosus GG:</h3>
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                    <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K216005">BBa_K216005</a>, <a href="http://parts.igem.org/Part:BBa_K2760002">BBa_K2760002</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760029">BBa_K2760029</a></p>
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<h3 align ="right" style="color:#00008B"> Strategy A:  L. rhamnosus GG  </h3>
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                </div>
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<img src="https://static.igem.org/mediawiki/2018/e/e3/T--TecMonterrey_GDL--ConstructReal3.png" align="right">
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              </div>
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<h3 align="left" style="color:#00008B"> Simbology </h3>
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<img src="https://static.igem.org/mediawiki/2018/4/48/T--TecMonterrey_GDL--Construct5.png" align="left">
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<h1></h1>
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<h1></h1>
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</div>
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    <div>
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<h2 style="color:#00008B" align="center" > Table C. Strategy A:  L. rhamnosus GG </h2>
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<h3 style="color:#00008B" align="center"> (all optimized for E. coli)</h3>
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−
<img src="https://static.igem.org/mediawiki/2018/e/ee/T--TecMonterrey_GDL--Table3.png">
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</div>
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  <div>
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              <div class="row">
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<h3 align="center"> For L. rhamnosus GG:</h3>
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                <div class="col-md-6">
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<h3 align ="right" style="color:#00008B"> Strategy B:  L. rhamnosus GG  </h3>
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                  <br><br><br><br><br><br><br><br><br>
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<img src="https://static.igem.org/mediawiki/2018/d/d7/T--TecMonterrey_GDL--ConstructReal4.png" align="right">
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                  <center>
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<h3 align="left" style="color:#00008B"> Simbology </h3>
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                  <h4><a href="http://parts.igem.org/Part:BBa_K2760011">Strategy B: Part:BBa_K2760011 - Plan B for Lactobachill</a></h4>
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<img src="https://static.igem.org/mediawiki/2018/4/48/T--TecMonterrey_GDL--Construct5.png" align="left">
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                </center><br>
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<h1></h1>
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                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/e/e0/T--TecMonterrey_GDL--Parts--3.png" alt="service-image">
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<h1></h1>
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</div>
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    <div>
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<h2 style="color:#00008B" align="center" > Table D. Strategy B: L. rhamnosus GG </h2>
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<h3 style="color:#00008B" align="center"> (all optimized for E. coli)</h3>
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<img src="https://static.igem.org/mediawiki/2018/a/a5/T--TecMonterrey_GDL--Table4.png">
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</div>
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  <div>
+
                  <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K206000">BBa_K206000</a>, <a href="http://parts.igem.org/Part:BBa_J34801">BBa_J34801</a>, <a href="http://parts.igem.org/Part:BBa_K2760023">BBa_K2760023</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>,
−
<h3 align="center"> Signaling peptides</h3>
+
                    <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K216005">BBa_K216005</a>, <a href="http://parts.igem.org/Part:BBa_K2760003">BBa_K2760003</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760029">BBa_K2760029</a></p>
−
<h3 align ="right" style="color:#00008B"> For E. Coli</h3>
+
                </div>
−
<img src="https://static.igem.org/mediawiki/2018/e/e0/T--TecMonterrey_GDL--ConstructReal5.png" align="right">
+
                <div class="col-md-6">
−
<h3 align="left" style="color:#00008B"> Simbology </h3>
+
                  <br>
−
<img src="https://static.igem.org/mediawiki/2018/4/48/T--TecMonterrey_GDL--Construct5.png" align="left">
+
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/d/de/T--TecMonterrey_GDL--Parts--Tabla2.png" alt="service-image">
−
<h1></h1>
+
                </div>
−
<h1></h1>
+
              </div>
−
<h1></h1>
+
−
<h1></h1>
+
−
</div>
+
−
    <div>
+
−
<h2 style="color:#00008B" align="center" > Table E. Sp1-Nsp4:  E. coli </h2>
+
−
<h3 style="color:#00008B" align="center"> (all optimized for E. coli)</h3>
+
−
<img src="https://static.igem.org/mediawiki/2018/9/93/T--TecMonterrey_GDL--Table5.png">
+
−
</div>
+
  
−
  <div>
+
              <br><br>
−
<h4 align="center" style="color:#00008B"> Signaling Peptides</h4>
+
                  <div class="section-title">
−
<h3 align="right"> Sp2-Nsp2 (E. coli):</h3>
+
                      <h3>For <i>L. rhamnosus GG</i></h3>
−
<img src="https://static.igem.org/mediawiki/2018/f/f0/T--TecMonterrey_GDL--ConstructReal6.png" align="right">
+
                  </div>
−
<h3 align="left" style="color:#00008B"> Simbology </h3>
+
              <div class="row">
−
<img src="https://static.igem.org/mediawiki/2018/4/48/T--TecMonterrey_GDL--Construct5.png" align="left">
+
                <div class="col-md-6">
−
<h1></h1>
+
                  <br>
−
<h1></h1>
+
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/84/T--TecMonterrey_GDL--Parts--Tabla3.png" alt="service-image">
−
</div>
+
                </div>
−
    <div>
+
                <div class="col-md-6">
−
<h2 style="color:#00008B" align="center" > Table F. Sp2-Nsp2:  E. coli </h2>
+
                  <br><br><br><br><br><br><br><br><br><br><br>
−
<h3 style="color:#00008B" align="center"> (all optimized for E. coli)</h3>
+
                  <center>
−
<img src="https://static.igem.org/mediawiki/2018/6/61/T--TecMonterrey_GDL--Table6.png">
+
                  <h4><a href="http://parts.igem.org/Part:BBa_K2760033">Strategy A:  Part: BBa_K2760033</a></h4>
−
</div>
+
                </center><br>
 +
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/b/bc/T--TecMonterrey_GDL--Parts--4.png" alt="service-image">
  
−
  <div>
+
                  <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760005">BBa_K2760005</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>, <a href="http://parts.igem.org/Part:BBa_K2760023">BBa_K2760023</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>,
−
<h3 align="center">Sp negative control (E. coli): </h3>
+
                    <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760007">BBa_K2760007</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K216005">BBa_K216005</a>, <a href="http://parts.igem.org/Part:BBa_K2760000">BBa_K2760000</a>,<a href="http://parts.igem.org/Part:BBa_K2760002">BBa_K2760002</a> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760029">BBa_K2760029</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760008">BBa_K2760008</a> </p>
−
<img src="https://static.igem.org/mediawiki/2018/a/a9/T--TecMonterrey_GDL--ConstructReal7.png" align="right">
+
                </div>
−
<h3 align="left" style="color:#00008B"> Simbology </h3>
+
              </div>
−
<img src="https://static.igem.org/mediawiki/2018/4/48/T--TecMonterrey_GDL--Construct5.png" align="left">
+
−
<h1></h1>
+
−
<h1></h1>
+
−
</div>
+
−
    <div>
+
−
<h2 style="color:#00008B" align="center" >Table G. Sp-negative control:  E. coli </h2>
+
−
<h3 style="color:#00008B" align="center"> (all optimized for E. coli)</h3>
+
−
<img src="https://static.igem.org/mediawiki/2018/5/56/T--TecMonterrey_GDL--Table7.png">
+
−
</div>
+
  
−
  <div>
+
              <div class="row">
−
<h3 align="center">Sp positive control (E. coli): </h3>
+
                <div class="col-md-6">
−
<img src="https://static.igem.org/mediawiki/2018/d/dd/T--TecMonterrey_GDL--ConstructReal8.png" align="right">
+
                  <br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
−
<h3 align="left" style="color:#00008B"> Simbology </h3>
+
                  <center>
−
<img src="https://static.igem.org/mediawiki/2018/4/48/T--TecMonterrey_GDL--Construct5.png" align="left">
+
                  <h4><a href="http://parts.igem.org/Part:BBa_K2760034">Plan B (L. rhamnosus): Part: BBa_K276004</a></h4>
−
<h1></h1>
+
                </center><br>
−
<h1></h1>
+
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/4/42/T--TecMonterrey_GDL--Parts--5.png" alt="service-image">
−
</div>
+
−
    <div>
+
−
<h2 style="color:#00008B" align="center" >Table H. Sp positive control: E. coli </h2>
+
−
<h3 style="color:#00008B" align="center"> (all optimized for E. coli)</h3>
+
−
<img src="https://static.igem.org/mediawiki/2018/2/22/T--TecMonterrey_GDL--Table8.png">
+
−
</div>
+
  
−
<div>
+
                  <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760005">BBa_K2760005</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>, <a href="http://parts.igem.org/Part:BBa_K2760023">BBa_K2760023</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>,
−
<h4 style="color:#00008B" align="center" > For L. rhamnosus: </h4>
+
                    <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760007">BBa_K2760007</a>, <a href="http://parts.igem.org/Part:BBa_K216005">BBa_K216005</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760000">BBa_K2760000</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760003">BBa_K2760003</a>,
−
<h3 align="right">Sp1-Nsp4 (L.rhamnosus): </h3>
+
                    <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760029">BBa_K2760029</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760008">BBa_K2760008</a></p>
−
<img src="https://static.igem.org/mediawiki/2018/6/67/T--TecMonterrey_GDL--ConstructReal9.png" align="right">
+
                </div>
−
<h3 align="left" style="color:#00008B"> Simbology </h3>
+
                <div class="col-md-6">
−
<img src="https://static.igem.org/mediawiki/2018/4/48/T--TecMonterrey_GDL--Construct5.png" align="left">
+
                  <br>
−
<h1></h1>
+
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/7/7d/T--TecMonterrey_GDL--Parts--Tabla4.png" alt="service-image">
−
<h1></h1>
+
                </div>
−
</div>
+
              </div>
−
    <div>
+
−
<h2 style="color:#00008B" align="center" >Table I. Sp1-Nsp4:  L. rhamnosus GG </h2>
+
−
<h3 style="color:#00008B" align="center"> (all optimized for E. coli)</h3>
+
−
<img src="https://static.igem.org/mediawiki/2018/c/cd/T--TecMonterrey_GDL--Table9.png">
+
−
</div>
+
  
−
<div>
+
              <br><br><br>
−
<h4 style="color:#00008B" align="center" > For L. rhamnosus: </h4>
+
              <div class="service-box section-title text-center">
−
<h3 align="right">Sp2-Nsp2 (L.rhamnosus): </h3>
+
                  <h3> <u>Signaling peptides </u>
−
<img src="https://static.igem.org/mediawiki/2018/a/ae/T--TecMonterrey_GDL--ConstructReal10.png" align="right">
+
                  </h3>
−
<h3 align="left" style="color:#00008B"> Simbology </h3>
+
                  <p style="text-align:center">For the excretion of recombinant proteins</p>
−
<img src="https://static.igem.org/mediawiki/2018/4/48/T--TecMonterrey_GDL--Construct5.png" align="left">
+
              </div>
−
<h1></h1>
+
              <br><br><br>
−
<h1></h1>
+
              <div class="section-title">
−
</div>
+
                  <h3>For <i>E.coli</i></h3>
−
    <div>
+
              </div>
−
<h2 style="color:#00008B" align="center" >Table J. Sp2-Nsp2:  L. rhamnosus GG </h2>
+
−
<h3 style="color:#00008B" align="center"> (all optimized for E. coli)</h3>
+
−
<img src="https://static.igem.org/mediawiki/2018/7/7e/T--TecMonterrey_GDL--Table10.png">
+
−
</div>
+
  
−
<div>
+
              <div class="row">
−
<h4 style="color:#00008B" align="center" > For L. rhamnosus: </h4>
+
                <div class="col-md-6">
−
<h3 align="right">Sp negative control (L.rhamnosus): </h3>
+
                  <br>
−
<img src="https://static.igem.org/mediawiki/2018/3/3f/T--TecMonterrey_GDL--ConstructReal11.png" align="right">
+
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/e/e6/T--TecMonterrey_GDL--Parts--Tabla5.png" alt="service-image">
−
<h3 align="left" style="color:#00008B"> Simbology </h3>
+
                </div>
−
<img src="https://static.igem.org/mediawiki/2018/4/48/T--TecMonterrey_GDL--Construct5.png" align="left">
+
                <div class="col-md-6">
−
<h1></h1>
+
<br><br>
−
<h1></h1>
+
                  <h4><a style="text-align:center" href="http://parts.igem.org/Part:BBa_K2760019:Design#References">Sp1-Nsp4 construct: BBa_K2760019 -
 +
Characterization construct for SGP1-NSP4 -</a></h4>
 +
                <br>
 +
<center>
 +
                  <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/3/33/T--TecMonterrey_GDL--Parts--6.png" alt="service-image">
 +
</center>
 +
 
 +
                  <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">BBa_I712074</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_Z0262">BBa_Z0262</a>, <a href="http://parts.igem.org/Part:BBa_K2760000">BBa_K2760000</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a></p>
 +
                </div>
 +
              </div>
 +
              <br><br>
 +
              <div class="row">
 +
                <div class="col-md-6">
 +
                  <br>
 +
                  <h4><a href="http://parts.igem.org/Part:BBa_K2760021:Design">BBa_K2760021 - SP2-Nsp2 Characterization construct</a></h4><br>
 +
<center>
 +
                  <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/a/a2/T--TecMonterrey_GDL--Parts--7.png" alt="service-image">
 +
</center>
 +
 
 +
                  <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">BBa_I712074</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_Z0262">BBa_Z0262</a>, <a href="http://parts.igem.org/Part:BBa_K2760004">BBa_K2760004</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a></p>
 +
                </div>
 +
                <div class="col-md-6">
 +
                  <br>
 +
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/0/05/T--TecMonterrey_GDL--Parts--Tabla6.png" alt="service-image">
 +
                </div>
 +
              </div>
 +
              <br><br>
 +
              <div class="row">
 +
                <div class="col-md-6">
 +
                  <br><br><br><br><br>
 +
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/b/b5/T--TecMonterrey_GDL--Parts--Tabla7.png" alt="service-image">
 +
                </div>
 +
                <div class="col-md-6">
 +
                  <br><br>
 +
                  <h4><a href="http://parts.igem.org/partsdb/edit_seq.cgi?part=BBa_K2760020">BBa_K2760020 -  Negative control for signal peptide characterization</a></h4>
 +
                <br>
 +
<center>
 +
                  <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/0/04/T--TecMonterrey_GDL--Parts--8.png" alt="service-image">
 +
</center>
 +
 
 +
                  <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">BBa_I712074</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_Z0262">BBa_Z0262</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a></p>
 +
                </div>
 +
              </div>
 +
              <br><br><br>
 +
              <div class="row">
 +
                <div class="col-md-6">
 +
                  <h4><a href="http://parts.igem.org/partsdb/edit_seq.cgi?part=BBa_K2760022">BBa_K2760022 -  Positive Control for Characterization of Signal Peptides (DsbAss)</a></h4>
 +
                <br>
 +
<center>
 +
                  <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/0/08/T--TecMonterrey_GDL--Parts--9.png" alt="service-image">
 +
</center>
 +
 
 +
                  <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">BBa_I712074</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_Z0262">BBa_Z0262</a>, <a href="http://parts.igem.org/Part:BBa_K2760001">BBa_K2760001</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a></p>
 +
                </div>
 +
                <div class="col-md-6">
 +
                  <br>
 +
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/3/31/T--TecMonterrey_GDL--Parts--Tabla8.png" alt="service-image">
 +
                </div>
 +
              </div>
 +
 
 +
              <br><br><br>
 +
              <div class="section-title">
 +
                  <h3>For <i>L.rhamnosus</i></h3>
 +
              </div>
 +
 
 +
              <div class="row">
 +
                <div class="col-md-6">
 +
                  <br>
 +
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/1/1a/T--TecMonterrey_GDL--Parts--Tabla9.png" alt="service-image">
 +
                </div>
 +
                <div class="col-md-6">
 +
                  <br><br><br><br><br><br>
 +
                  <h4><a href="http://parts.igem.org/Part:BBa_K2760030">Sp1-Nsp4- Characterization construct Part: BBa_K2760030</a></h4>
 +
                <br>
 +
<center>
 +
                  <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/a/a1/T--TecMonterrey_GDL--Parts--10.png" alt="service-image">
 +
</center>
 +
 
 +
                  <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760009">BBa_K2760009</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760005">BBa_K2760005</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760000">BBa_K2760000</a>,
 +
                    <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760029">BBa_K2760029</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760007">BBa_K2760007</a></p>
 +
                </div>
 +
              </div>
 +
              <br><br>
 +
              <div class="row">
 +
                <div class="col-md-6">
 +
                  <br><br><br><br><br><br>
 +
                  <h4><a href="http://parts.igem.org/Part:BBa_K2760031:Design#References">Sp2-Nsp2- Characterization Construct Part: BBa_K2760031</a></h4>
 +
                <br>
 +
<center>
 +
                  <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/0/09/T--TecMonterrey_GDL--Parts--11.png" alt="service-image">
 +
</center>
 +
 
 +
                  <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760009">BBa_K2760009</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760005">BBa_K2760005</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>, <a href="http://parts.igem.org/Part:BBa_K2760004">BBa_K2760004</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760029">BBa_K2760029</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760007">BBa_K2760007</a></p>
 +
                </div>
 +
                <div class="col-md-6">
 +
                  <br>
 +
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/6/66/T--TecMonterrey_GDL--Parts--Tabla10.png" alt="service-image">
 +
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 +
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 +
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 +
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 +
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 +
                  <br>
 +
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/c/cf/T--TecMonterrey_GDL--Parts--Tabla11.png" alt="service-image">
 +
                </div>
 +
                <div class="col-md-6">
 +
                  <br><br><br>
 +
                  <h4><a href="http://parts.igem.org/Part:BBa_K2760032">Sp negative control construct: Part BBa_2760032</a></h4>
 +
                <br>
 +
<center>
 +
                  <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/3/37/T--TecMonterrey_GDL--Parts--12.png" alt="service-image">
 +
</center>
 +
 
 +
                  <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760009">BBa_K2760009</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760005">BBa_K2760005</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>,
 +
                    <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760029">BBa_K2760029</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760007">BBa_K2760007</a></p>
 +
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−
    <div>
+
 
−
<h2 style="color:#00008B" align="center" >Table K. Sp negative control:  L. rhamnosus GG</h2>
+
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<h3 style="color:#00008B" align="center"> (all optimized for E. coli)</h3>
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Latest revision as of 02:34, 18 October 2018

TecMonterrey_GDL

Parts

For E.coli

In the following table we present the parts we have used for our plasmid construction, detailed in Construct design, the parts we used are classified as they appear in our composite constructs:


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Strategy A : Part:BBa_K2760010 - Lactobachill Plan A (E. coli)


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Sub parts: BBa_K206000, BBa_J34801, BBa_K2760023, BBa_K2760024, BBa_B0015, BBa_K216005, BBa_K2760002, BBa_K2760012, BBa_K2760029










Strategy B: Part:BBa_K2760011 - Plan B for Lactobachill


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Sub parts: BBa_K206000, BBa_J34801, BBa_K2760023, BBa_K2760024, BBa_B0015, BBa_K216005, BBa_K2760003, BBa_K2760012, BBa_K2760029


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For L. rhamnosus GG


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Strategy A: Part: BBa_K2760033


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Sub parts: BBa_K2760005, BBa_K2760006, BBa_K2760023, BBa_K2760024, BBa_K2760007, BBa_K216005, BBa_K2760000,BBa_K2760002 BBa_K2760012, BBa_K2760029, BBa_K2760008
















Plan B (L. rhamnosus): Part: BBa_K276004


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Sub parts: BBa_K2760005, BBa_K2760006, BBa_K2760023, BBa_K2760006, BBa_K2760024, BBa_K2760007, BBa_K216005, BBa_K2760000, BBa_K2760003, BBa_K2760012, BBa_K2760029, BBa_K2760008


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Signaling peptides

For the excretion of recombinant proteins




For E.coli


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Sp1-Nsp4 construct: BBa_K2760019 - Characterization construct for SGP1-NSP4 -


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Sub parts: BBa_I712074, BBa_Z0262, BBa_K2760000, BBa_K2760024, BBa_K2760012, BBa_B0015




BBa_K2760021 - SP2-Nsp2 Characterization construct


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Sub parts: BBa_I712074, BBa_Z0262, BBa_K2760004, BBa_K2760024, BBa_K2760012, BBa_B0015


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BBa_K2760020 - Negative control for signal peptide characterization


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Sub parts: BBa_I712074, BBa_Z0262, BBa_K2760024, BBa_K2760012, BBa_B0015




BBa_K2760022 - Positive Control for Characterization of Signal Peptides (DsbAss)


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Sub parts: BBa_I712074, BBa_Z0262, BBa_K2760001, BBa_K2760024, BBa_K2760012, BBa_B0015


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For L.rhamnosus


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Sp1-Nsp4- Characterization construct Part: BBa_K2760030


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Sub parts: BBa_K2760009, BBa_K2760005, BBa_K2760006, BBa_K2760000, BBa_K2760029, BBa_K2760007









Sp2-Nsp2- Characterization Construct Part: BBa_K2760031


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Sub parts: BBa_K2760009, BBa_K2760005, BBa_K2760006, BBa_K2760004, BBa_K2760029, BBa_K2760007


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Sp negative control construct: Part BBa_2760032


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Sub parts: BBa_K2760009, BBa_K2760005, BBa_K2760006, BBa_K2760029, BBa_K2760007

TecMonterrey_GDL