Difference between revisions of "Team:Jiangnan China/Description"

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{{Jiangnan_China}}
 
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            <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Description">Description</a>
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            <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Design">Design</a>
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            <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Experiments">Experiments</a>
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<h1>Description</h1>
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      <img src="https://static.igem.org/mediawiki/2018/9/9b/T--jiangnan_china--description--1.jpg" width="100%">
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      <div class="dcpt3" style="font-size:20px;line-height:1.5;font-family: 'spr';">
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
 
  
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      <div class="dcpt3" style="font-size:20px;line-height:1.5;font-family: 'spr';margin-top: -50px;">
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    <div align="left" style="font-family: 'spr';font-size:40px;border-bottom:2px solid #584b4f;"><strong>Background</strong></div>
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    <br><br>
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      &nbsp;&nbsp;&nbsp;&nbsp;Over the past two decades, <span class="font-italic">Lactococcus lactis</span> has vastly extended its application from food to being a successful <a href="https://en.wikipedia.org/wiki/Microbial_cell_factory" target="_blank">microbial cell factory</a>.Not only can it produce lactic acid, vitamins, bacteriocins and antimicrobial peptide (nisin) in traditional fermentation, it can also express heterologous antigens, thus allowing the bacteria to act as live vaccines. Using <span class="font-italic">L. lactis</span> as vaccine carriers is appealing as it is able to induce both mucosal and systemic immune responses, has adjuvant properties, and is free from risks associated with the use of conventional attenuated live pathogens. With lyophilization, <span class="font-italic">L. lactis</span> does not require storage at low temperature, and administration of the preparation does not require specialized personnel.<br>
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      &nbsp;&nbsp;&nbsp;&nbsp;However, <span class="font-italic">L. lactis</span> suffers from various stress conditions including acid, osmotic and oxygen during fermentation processing, among which acid stress and cold stress are great survival challenges. During fermentation, the decrease of environmental pH will inhibit the activity of intracellular enzymes, which will also affect the normal growth and metabolism of microbial cells. Furthermore, as live vaccines, <span class="font-italic">L.lactis</span> has an advantage in the low pH condition of gastric juice compared with other chassis microorganism. During the process of lyophilization, it suffers from severe cold stress, which rapidly reduce their survival rate. Therefore, the strain resistant to acid and cold needs to be constructed as soon as possible.
  
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      <br><br>
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<h3>What should this page contain?</h3>
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<ul>
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<li> A clear and concise description of your project.</li>
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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<li>References and sources to document your research.</li>
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<li>Use illustrations and other visual resources to explain your project.</li>
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<h3>Inspiration</h3>
 
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<p>See how other teams have described and presented their projects: </p>
 
  
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<ul>
 
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<li><a href="https://2016.igem.org/Team:Imperial_College/Description">2016 Imperial College</a></li>
 
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<li><a href="https://2016.igem.org/Team:Wageningen_UR/Description">2016 Wageningen UR</a></li>
 
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> 2014 UC Davis</a></li>
 
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">2014 SYSU Software</a></li>
 
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</div>
 
  
  
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      <div id="dcpt4" style="font-size:20px;line-height:1.5;font-family: 'spr';">
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    <div align="left" style="font-family: 'spr';font-size:40px;border-bottom:2px solid #584b4f;"><strong>What are we doing</strong></div>
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    <br>
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    <br>
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      &nbsp;&nbsp;&nbsp;&nbsp;Therefore, we aim to create an ideal chassis microorganism with good resistance and food-safe characteristics by synthetic biology methods. We added genes <span class="font-italic">msmk</span>, <span class="font-italic">cspD2</span>, promoter <span class="font-italic">ppepN</span>, <span class="font-italic">gfp</span> and a kill-switch (→<a href="https://2018.igem.org/Team:Jiangnan_China/Parts" >Basic parts</a>) respectively to model strain <span class="font-italic">Lactococcus lactis</span> NZ3900 to give it the anti-acid, anti-cold characteristics.
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      <br><br>
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      <p style="text-align: center;"><a href="https://2018.igem.org/Team:Jiangnan_China/Design" class="btn btn-info">Click here for more details. </a></p>
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      <br>
  
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      </div>
  
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<h3>Advice on writing your Project Description</h3>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be concise, accurate, and unambiguous in your achievements.
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<h3>References</h3>
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
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        <a href="http://www.jiangnan.edu.cn/" title="江南大学"><img src="https://static.igem.org/mediawiki/2018/1/1d/T--jiangnan_china--jiangnanlogo.png"></a>
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        <a href="http://biotech.jiangnan.edu.cn/" title="江南大学生物工程学院"><img src="https://static.igem.org/mediawiki/2018/a/a6/T--jiangnan_china--shenggonglogo.png"></a>
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Latest revision as of 02:44, 18 October 2018

Background


    Over the past two decades, Lactococcus lactis has vastly extended its application from food to being a successful microbial cell factory.Not only can it produce lactic acid, vitamins, bacteriocins and antimicrobial peptide (nisin) in traditional fermentation, it can also express heterologous antigens, thus allowing the bacteria to act as live vaccines. Using L. lactis as vaccine carriers is appealing as it is able to induce both mucosal and systemic immune responses, has adjuvant properties, and is free from risks associated with the use of conventional attenuated live pathogens. With lyophilization, L. lactis does not require storage at low temperature, and administration of the preparation does not require specialized personnel.
    However, L. lactis suffers from various stress conditions including acid, osmotic and oxygen during fermentation processing, among which acid stress and cold stress are great survival challenges. During fermentation, the decrease of environmental pH will inhibit the activity of intracellular enzymes, which will also affect the normal growth and metabolism of microbial cells. Furthermore, as live vaccines, L.lactis has an advantage in the low pH condition of gastric juice compared with other chassis microorganism. During the process of lyophilization, it suffers from severe cold stress, which rapidly reduce their survival rate. Therefore, the strain resistant to acid and cold needs to be constructed as soon as possible.

What are we doing


    Therefore, we aim to create an ideal chassis microorganism with good resistance and food-safe characteristics by synthetic biology methods. We added genes msmk, cspD2, promoter ppepN, gfp and a kill-switch (→Basic parts) respectively to model strain Lactococcus lactis NZ3900 to give it the anti-acid, anti-cold characteristics.

Click here for more details.


Copyright © jiangnan_China 2018