Difference between revisions of "Team:Munich"

 
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         </div>
 
         <div class="col-12 col-md-6 slide-in pt-3" style="padding-right: 6vw;">
 
         <div class="col-12 col-md-6 slide-in pt-3" style="padding-right: 6vw;">
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             <p class="text-justify text-phactory2 mobileText">Phages are capable of specifically and reliably infecting and killing bacteria but are harmless to human cells. They can therefore be administered to patients to cure bacterial infections. This process is called:</p>
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             <p class="text-justify text-phactory2 mobileText">Phages are capable of specifically and reliably infecting and killing bacteria but are harmless to human cells. They can therefore be administered to patients as a cure for bacterial infections. This process is called:</p>
 
             <h2 class="text-phactory2 display-4 mobileTitle">Phage Therapy</h2>
 
             <h2 class="text-phactory2 display-4 mobileTitle">Phage Therapy</h2>
 
         </div>
 
         </div>
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         <div class="row p-5" style="padding-left: 10vw; padding-right: 10vw;">
 
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             <div class="col-12 col-md-6 slide-in">
 
             <div class="col-12 col-md-6 slide-in">
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                 <img class="img-fluid" src="https://static.igem.org/mediawiki/2018/a/a6/T--munich--oldmethod_icon%282%29.png" alt="meetUp" style="padding-left: 6vw;">
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                 <img class="img-fluid" src="https://static.igem.org/mediawiki/2018/7/72/T--Munich--Home_traditional_new.png" alt="meetUp" style="padding-left: 6vw;">
 
             </div>
 
             </div>
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             <div class="col-12 col-md-6 mt-5 slide-in">
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                <h2 class="text-phactory2 display-4 mobileTitle">Traditional Method</h2>
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             <div class="col-12 col-md-6 slide-in pt-3" style="padding-right: 6vw;">
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                 <h4 class="text-phactory2">hazardous<br>impure<br>host restricted</h4>
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<h2 class="text-phactory2 display-4 mobileTitle">Traditional Methods</h2>
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                 <p class="text-justify text-phactory2 mobileText">...are unsafe for manufacturer and patient. Giant flasks of pathogenic bacteria are cultivated in order to isolate therapeutic bacteriophages.</p>  
 
             </div>
 
             </div>
 
         </div>
 
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     <div id="conceptFlow" class="text-dark bg-whites">
 
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         <div class="container">
 
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             <div id="conceptFlowTitle" class="pb-3 pt-5 mb-3 text-center" style="">How does <span class="text-phactory1 display-2 mobileTitle h2">Phactory</span> work?</div>
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             <div id="conceptFlowTitle" class="pb-3 pt-5 mb-3 text-center slide-in">How does <span class="text-phactory1 display-2 mobileTitle h2">Phactory</span> work?</div>
 
              
 
              
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                 <div class="row bg-white text-dark pb-5 align-items-center" style="">
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                 <div class="row bg-white text-dark pb-5 align-items-center slide-in">
 
                 <div class="col-12 text-center">
 
                 <div class="col-12 text-center">
 
                 <img class="img-fluid" src="https://static.igem.org/mediawiki/2018/6/6a/T--munich--newmethod_icon.png" alt="meetUp" style="">
 
                 <img class="img-fluid" src="https://static.igem.org/mediawiki/2018/6/6a/T--munich--newmethod_icon.png" alt="meetUp" style="">
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                 <div class="col-8 col-md-6 text-center">
 
                 <div class="col-8 col-md-6 text-center">
 
                     <h5>Diagnosis</h5>
 
                     <h5>Diagnosis</h5>
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                     <p class="text-justify">Next generation diagnostic systems like SHERLOCK can identify pathogens with high precision and accuracy. This enables rapid identification of an individualized therapeutic strategy even in the case of multiresistant superbugs. Using Phactory, we overcome the last remaining obstacles for an efficient bacteriophage therapy.</p>
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                     <p class="text-justify">Next generation diagnostic systems like SHERLOCK can identify pathogens with high precision and accuracy. This enables rapid identification of an individualized therapeutic strategy even in the case of multiresistant superbugs. Using Phactory, we overcome the last remaining obstacles for efficient bacteriophage therapy.</p>
 
                 </div>
 
                 </div>
 
             </div>
 
             </div>
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             <div class="row align-items-center justify-content-end how-it-works d-flex">
 
             <div class="row align-items-center justify-content-end how-it-works d-flex">
 
                 <div class="col-8 col-md-6 text-center">
 
                 <div class="col-8 col-md-6 text-center">
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                     <h5>Phage Library Selection</h5>
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                     <h5>Modular Phage Assembly</h5>
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                     <p class="text-justify">By using a cell-free production system, we utilize one of the most revolutionary concepts of synthetic biology to produce therapeutic bacteriophages. Based on a personalized diagnosis, bacteriophage DNA is chosen from a library and is subjected to extensive quality assessment procedures to ensure a safe and effective application.</p>
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                     <p class="text-justify">With a cell-free production system we utilize one of the most revolutionary concepts of synthetic biology to produce therapeutic bacteriophages. Based on personalized diagnostics, bacteriophage DNA is chosen from a library and subjected to extensive quality assessment procedures to ensure a safe and effective application.</p>
 
                     <div class="labelBox">
 
                     <div class="labelBox">
 
                     <button class="reg">homogeneous</button>
 
                     <button class="reg">homogeneous</button>
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                         <span>✖</span>
 
                         <span>✖</span>
 
                         <h1>high quality</h1>
 
                         <h1>high quality</h1>
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                         <p>The phage genome DNA is produced using extensive purification protocols, resulting in high quality raw materials with minimal contamination.</p>
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                         <p>The phage genome DNA is extensively purified, resulting in a high quality template with minimal contamination.</p>
 
                         </div>
 
                         </div>
 
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                 <div class="col-8 col-md-6 text-center">
 
                 <div class="col-8 col-md-6 text-center">
 
                     <h5>Synthetic Phage Manufacturing</h5>
 
                     <h5>Synthetic Phage Manufacturing</h5>
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                     <p class="text-justify">Production of our bacteriophages in a cell-free system ensures a fast and dependable application. Furthermore, working in a cell-free environment enables host independent production of phages in only 2.5% of the traditional production volume while demanding no biosafety regulations.</p>
+
                     <p class="text-justify">Production of bacteriophages in a cell-free system ensures a fast and reliable application. Furthermore, working in a cell-free environment enables host independent production of phages in only 2.5% of the conventional production volume while demanding no biosafety regulations.</p>
 
                     <div class="labelBox">
 
                     <div class="labelBox">
 
                     <button class="reg"> affordable </button>
 
                     <button class="reg"> affordable </button>
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                         <span>✖</span>
 
                         <span>✖</span>
 
                         <h1>affordable</h1>
 
                         <h1>affordable</h1>
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                         <p>Below $10 per treatment, Phactory has a very competitive market value.</p>
+
                         <p>At less than $10 per treatment, Phactory allows for a highly competitive production price.</p>
 
                         </div>
 
                         </div>
 
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                         <span>✖</span>
 
                         <span>✖</span>
 
                         <h1>scalable</h1>
 
                         <h1>scalable</h1>
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                         <p>Manufacturing in Phactory can be scaled up for mass production.</p>
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                         <p>Manufacturing in Phactory can be scaled up for bulk production.</p>
 
                         </div>
 
                         </div>
 
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                         <span>✖</span>
 
                         <span>✖</span>
 
                         <h1>modular</h1>
 
                         <h1>modular</h1>
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                         <p>Phactory is an open system. This allows for the incorporation of modified proteins while leaving the genome unmodified, thereby achieving modularity without GMOs.</p>
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                         <p>Phactory is an open system. This allows for the incorporation of modified proteins while leaving the genome unmodified, thereby achieving modularity without genetic modifications.</p>
 
                         </div>
 
                         </div>
 
                     </div>
 
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                 <div class="col-8 col-md-6 text-center">
 
                 <div class="col-8 col-md-6 text-center">
 
                     <h5>Quality Control</h5>
 
                     <h5>Quality Control</h5>
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                     <p class="text-justify">Extensive quality controls are employed to make sure that the produced bacteriophages are pure and effective. Potential complications due to toxins and contaminants are eliminated.</p>
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                     <p class="text-justify">A thorough quality control structure assures that the produced bacteriophages are pure and effective. Risk of toxic contamination is eliminated.</p>
 
                     <div class="labelBox">
 
                     <div class="labelBox">
 
                     <button class="reg">pure</button>
 
                     <button class="reg">pure</button>
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                         <span>✖</span>
 
                         <span>✖</span>
 
                         <h1>pure</h1>
 
                         <h1>pure</h1>
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                         <p>Purity is essential for any therapeutic application. We use next-generation sequencing to ensure the absence of any contaminating DNA in the manufacturing process.</p>
+
                         <p>Purity is essential for any therapeutic application. We use third generation sequencing to guarantee absence of DNA contamination in the manufacturing process.</p>
 
                         </div>
 
                         </div>
 
                     </div>
 
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                         <span>✖</span>
 
                         <span>✖</span>
 
                         <h1>toxin-free</h1>
 
                         <h1>toxin-free</h1>
−
                         <p>Phactory assembles phages outside of living cells and is therefore free of most biological contaminants. We engineered it to reduce immunogenicity in humans by five orders of magnitude.</p>
+
                         <p>Phactory assembles phages outside of living cells and is therefore free of most biological contaminants. We engineered the manufacturing system to reduce immunogenicity in humans by several orders of magnitude.</p>
 
                         </div>
 
                         </div>
 
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                         <span>✖</span>
 
                         <span>✖</span>
 
                         <h1>GMO-free</h1>
 
                         <h1>GMO-free</h1>
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                         <p>The phages assembled by Phactory are purified to remove any genetically modified material before ever leaving a controlled lab environment.</p>
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                         <p>The phages assembled by Phactory are purified to remove any genetically modified material before leaving a controlled lab environment.</p>
 
                         </div>
 
                         </div>
 
                     </div>
 
                     </div>
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                 <div class="col-8 col-md-6 text-center">
 
                 <div class="col-8 col-md-6 text-center">
 
                     <h5>Packaging</h5>
 
                     <h5>Packaging</h5>
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                     <p class="text-justify">Bacteriophage therapy covers a broad range of potential applications. We engineered custom hardware to package bacteriophages for oral treatment. Our packaging procedure is the last step of a personalized on-site treatment process.</p>
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                     <p class="text-justify">Bacteriophage therapy covers a broad range of potential applications. We engineered custom hardware to package bacteriophages for oral treatment. Our packaging procedure is the last step of a personalized on-site manufacturing process.</p>
 
                     <div class="labelBox">
 
                     <div class="labelBox">
 
                     <button class="reg">long shelf-life</button>
 
                     <button class="reg">long shelf-life</button>
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                         <span>✖</span>
 
                         <span>✖</span>
 
                         <h1>on-site</h1>
 
                         <h1>on-site</h1>
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                         <p>Producing phages in Phactory is so easy that it allows any doctor to apply phage therapy on-site.</p>
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                         <p>Producing phages in Phactory is so simple that it allows any doctor to apply phage therapy on-site.</p>
 
                         </div>
 
                         </div>
 
                     </div>
 
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                     <span> | </span>   
 
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                     <div class="labelBox">
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                     <button class="reg">integrated human practice</button>
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                     <button class="reg">integrated human practices</button>
 
                         <div class="pop text-left">
 
                         <div class="pop text-left">
 
                         <span>✖</span>
 
                         <span>✖</span>
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                         <span>✖</span>
 
                         <span>✖</span>
 
                         <h1>ready</h1>
 
                         <h1>ready</h1>
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                         <p>In cooperation with phage therapy pioneers in Belgium, we safely produced functional phages against EHEC – a severe intestinal infection.</p>
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                         <p>In cooperation with phage therapy pioneers in Belgium, we safely produced functional phages against EHEC – a deadly pathogen.</p>
 
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                 <img class="img-fluid border rounded" src="https://static.igem.org/mediawiki/2018/b/ba/T--Munich--europeanmeetup_teaser.jpeg" alt="meetUp" style="">
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                 <video controls poster="https://static.igem.org/mediawiki/2018/c/c8/T--Munich--Doctor_preview.jpg" style="width:100%;">
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<source src="https://static.igem.org/mediawiki/2018/f/ff/T--Munich--Intervie_RDI.mp4">
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</video>
 
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            <div class="col-12 col-md-6 p-5">
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                <h1>Human Practices</h1>
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                <span class="d-block mt-5">
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                <p>
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                    To get an overview of how serious antimicrobial resistance really is, we visited the hospital <i>Rechts der Isar</i> of the Technical University of Munich to meet Dr. Friedemann Gebhardt, the head of the hygiene department. Following his suggestions, we integrated storage protocols into Phactory.  </p></span>
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 +
                <a href="https://2018.igem.org/Team:Munich/Human_Practices" class="readButton btn btn-outline-info mt-5">Read More</a>
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             <div class="col-12 col-md-6">
 
             <div class="col-12 col-md-6">
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                <figure class="figure">
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    <img src="https://static.igem.org/mediawiki/2018/c/c7/T--munich--afterigem.png" class="figure-img img-fluid rounded" alt="A generic square placeholder image with rounded corners in a figure.">
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    </figure>
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            </div>
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            <div class="col-12 col-md-6 p-5">
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                <h1>Entrepreneurship</h1>
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                <span class="d-block mt-5">
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                <p>
 +
                    We set a timeline including the steps that have to be taken to further advance our project. Taking the disruptive potential of Phactory into account, we evaluated the penetrable market of Phactory. Parts of our project are patent-pending.</p></span>
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 +
                <a href="https://2018.igem.org/Team:Munich/Entrepreneurship" class="readButton btn btn-outline-info mt-5">Read More</a>
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                <figure class="figure">
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    <img src="https://static.igem.org/mediawiki/2018/8/88/T--Munich--Software_Sequ-Into-white.png" class="figure-img img-fluid rounded" alt="A generic square placeholder image with rounded corners in a figure.">
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                <h1>Sequ-Into</h1>
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                <span class="d-block mt-5">
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                <p>
 +
                    Our application combines powerful bioinformatic algorithms with third generation sequencing techniques, allowing us to reach DNA purity levels of up to 96%.</p></span>
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                <a href="https://2018.igem.org/Team:Munich/Software" class="readButton btn btn-outline-info mt-5">Read More</a>
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                <img class="img-fluid border rounded" src="https://static.igem.org/mediawiki/2018/b/ba/T--Munich--europeanmeetup_teaser.jpeg" alt="meetUp" style="">
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            </div>
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            <div class="col-12 col-md-6 p-5">
 
                 <h1>European Meetup</h1>
 
                 <h1>European Meetup</h1>
 
                 <h3>Munich 2018</h3>
 
                 <h3>Munich 2018</h3>
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Latest revision as of 02:45, 18 October 2018

Phactory

Phactory
Manufacturing Bacteriophages for Precision Medicine
Pathogenic Bacteria Turn Drug-Resistant at an Alarming Rate
By 2050, we project 10 million deaths per year caused by bacterial infections

UK Government (2015)
New superbug breaks through last line of antibiotic defense

Nature Microbiology (2018)
An alternative should be quality-assured, safe and effective

WHO (2015)
Phages Are the Natural Enemies of Bacteria
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Phages are capable of specifically and reliably infecting and killing bacteria but are harmless to human cells. They can therefore be administered to patients as a cure for bacterial infections. This process is called:

Phage Therapy

but...
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Traditional Methods

...are unsafe for manufacturer and patient. Giant flasks of pathogenic bacteria are cultivated in order to isolate therapeutic bacteriophages.

How does Phactory work?
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Diagnosis

Next generation diagnostic systems like SHERLOCK can identify pathogens with high precision and accuracy. This enables rapid identification of an individualized therapeutic strategy even in the case of multiresistant superbugs. Using Phactory, we overcome the last remaining obstacles for efficient bacteriophage therapy.

Modular Phage Assembly

With a cell-free production system we utilize one of the most revolutionary concepts of synthetic biology to produce therapeutic bacteriophages. Based on personalized diagnostics, bacteriophage DNA is chosen from a library and subjected to extensive quality assessment procedures to ensure a safe and effective application.

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homogeneous

Because our raw material is DNA, phage therapy based on well-defined phages is no longer a fantasy.

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software-aided

State of the art DNA sequencing tools are combined with bioinformatics to precisely determine the amount of contaminants in a sample.

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high quality

The phage genome DNA is extensively purified, resulting in a high quality template with minimal contamination.

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Synthetic Phage Manufacturing

Production of bacteriophages in a cell-free system ensures a fast and reliable application. Furthermore, working in a cell-free environment enables host independent production of phages in only 2.5% of the conventional production volume while demanding no biosafety regulations.

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affordable

At less than $10 per treatment, Phactory allows for a highly competitive production price.

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scalable

Manufacturing in Phactory can be scaled up for bulk production.

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modular

Phactory is an open system. This allows for the incorporation of modified proteins while leaving the genome unmodified, thereby achieving modularity without genetic modifications.

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safe

Host independence eliminates the need to cultivate pathogens to produce bacteriophages.

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Quality Control

A thorough quality control structure assures that the produced bacteriophages are pure and effective. Risk of toxic contamination is eliminated.

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pure

Purity is essential for any therapeutic application. We use third generation sequencing to guarantee absence of DNA contamination in the manufacturing process.

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toxin-free

Phactory assembles phages outside of living cells and is therefore free of most biological contaminants. We engineered the manufacturing system to reduce immunogenicity in humans by several orders of magnitude.

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GMO-free

The phages assembled by Phactory are purified to remove any genetically modified material before leaving a controlled lab environment.

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Packaging

Bacteriophage therapy covers a broad range of potential applications. We engineered custom hardware to package bacteriophages for oral treatment. Our packaging procedure is the last step of a personalized on-site manufacturing process.

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long shelf-life

Phactory and phages are fragile components. We combined various techniques to enable phage assembly outside of a lab.

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portable

We demonstrated lyophilization of Phactory components in order to enable long range transport.

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on-site

Producing phages in Phactory is so simple that it allows any doctor to apply phage therapy on-site.

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Therapy

Our bacteriophages are now ready for therapy. Phactory is fast and reliable enough to be an oral bedside application.

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cooperation

We were able to produce genetically modified phages we received from the iGEM Team in Grenoble.

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integrated human practice

We reached out to local doctors to demonstrate just how easy bacteriophage therapy using Phactory is and to gain valuable input for our project.

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ready

In cooperation with phage therapy pioneers in Belgium, we safely produced functional phages against EHEC – a deadly pathogen.

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Human Practices

To get an overview of how serious antimicrobial resistance really is, we visited the hospital Rechts der Isar of the Technical University of Munich to meet Dr. Friedemann Gebhardt, the head of the hygiene department. Following his suggestions, we integrated storage protocols into Phactory.

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Entrepreneurship

We set a timeline including the steps that have to be taken to further advance our project. Taking the disruptive potential of Phactory into account, we evaluated the penetrable market of Phactory. Parts of our project are patent-pending.

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Sequ-Into

Our application combines powerful bioinformatic algorithms with third generation sequencing techniques, allowing us to reach DNA purity levels of up to 96%.

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European Meetup

Munich 2018

One of the largest iGEM Events in Europe
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37
Teams
244
Participants
9
International speakers
11
Workshops