Difference between revisions of "Team:ECUST/Safety"

(Prototype team page)
 
 
(4 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
{{ECUST}}
+
{{ECUST/Header}}
 
<html>
 
<html>
 +
<div class="stars"></div>
 +
<div id="pagebanner" style="background-image:url(https://static.igem.org/mediawiki/2018/d/d0/T--ECUST--biosafetybanner.png);">
 +
<div id="bannerspace">
 +
   
 +
    <div id="bannerquote">Overview</div>
 +
   
 +
 +
</div>
 +
<main>
  
  
−
<div class="column full_size">
+
<div class="contentbox">
 +
<h1 class="box-heading">Lab</h1>
 +
<figure>
 +
<figure class="makeresponsive floatleft" style="width: 50%;">
 +
<img src="https://static.igem.org/mediawiki/2018/e/e2/T--ECUST--Safety1.1.png" alt="Figure1.1 OD600 reference point tab" class="zoom">
 +
</figure>
 +
<figure class="makeresponsive floatleft" style="width: 50%;">
 +
<img src="https://static.igem.org/mediawiki/2018/3/3f/T--ECUST--Safety1.2.png" alt="Figure1.1 OD600 reference point tab" class="zoom">
 +
</figure>
 +
<figure class="makeresponsive floatleft" style="width: 50%;">
 +
<img src="https://static.igem.org/mediawiki/2018/f/f8/T--ECUST--Safety1.3.jpg" alt="Figure1.1 OD600 reference point tab" class="zoom">
 +
</figure>
 +
<figure class="makeresponsive floatleft" style="width: 50%;">
 +
<img src="https://static.igem.org/mediawiki/2018/2/25/T--ECUST--Safety1.4.jpg" alt="Figure1.1 OD600 reference point tab" class="zoom">
 +
</figure>
 +
</figure>
 +
<p>During the whole process of our project, we do experiments in a low-risk-level lab (Level 1), and experiments are carried out in the open bench and biosafety cabinet. Before and during the experiments, we are strictly trained by our supervisors on safety rules to ensure we will obey the rules. We choose E.coli as our chassis, which is one of the most-widely used safe strains in synthetic biology, and it will not do any harm to human or environment.</p>
 +
</div>
  
−
<h1> Safety </h1>
 
−
<p>Please visit the <a href="https://2018.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
 
  
−
<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
+
<div class="contentbox">
 +
<h1 class="box-heading">Hardware</h1>
 +
<figure>
 +
<figure class="makeresponsive floatleft" style="width: 50%;">
 +
<img src="https://static.igem.org/mediawiki/2018/1/11/T--ECUST--Safety2.1.jpg" class="zoom">
 +
</figure>
  
 +
<p>With 4 different sensors, the device can collect related data on temperature, pH, oxidization-reduction potential (ORP) and electrical conductivity of the cooling water. The implemented blue LED light trigger the expression of suicide gene, which can be turned on or off manually. That is to say, if people think it’s time to renew the recycle cooling water or the risks of blockage and corrosion are removed, they can kill the engineered E. coli by turning on the blue light manually to ensure the highest level of biosafety.</p>
 
</div>
 
</div>
  
  
−
<div class="column two_thirds_size">
+
<div class="contentbox">
−
<h3>Safe Project Design</h3>
+
<h1 class="box-heading">Discussion </h1>
−
 
+
<figure>
−
<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
+
<figure class="makeresponsive floatleft" style="width: 50%;">
−
 
+
<img src="https://static.igem.org/mediawiki/2018/1/11/T--ECUST--Safety3.1.jpg" alt="Figure1.1 OD600 reference point tab" class="zoom">
−
<ul>
+
</figure>
−
<li>Choosing a non-pathogenic chassis</li>
+
−
<li>Choosing parts that will not harm humans / animals / plants</li>
+
−
<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
+
−
<li>Including an "induced lethality" or "kill-switch" device</li>
+
−
</ul>
+
  
 +
<p>On top of being trained by supervisors on biosafety rules, Iron Guardian will also gather together to have discussion on biosafety issues. Based on the Safety Form we filled before, when we have problems or make progress in the lab, we will seriously reflect from the view of biosafety, in order that we can keep improving ourselves.</p>
 
</div>
 
</div>
  
−
<div class="column third_size">
 
−
<h3>Safe Lab Work</h3>
 
  
−
<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
+
</main>
−
 
+
−
 
+
−
<h3>Safe Shipment</h3>
+
−
 
+
−
<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
+
 
</div>
 
</div>
−
 
  
 
</html>
 
</html>
 +
{{ECUST/SubFooter}}

Latest revision as of 02:58, 18 October 2018

Overview

Lab

Figure1.1 OD600 reference point tab
Figure1.1 OD600 reference point tab
Figure1.1 OD600 reference point tab
Figure1.1 OD600 reference point tab

During the whole process of our project, we do experiments in a low-risk-level lab (Level 1), and experiments are carried out in the open bench and biosafety cabinet. Before and during the experiments, we are strictly trained by our supervisors on safety rules to ensure we will obey the rules. We choose E.coli as our chassis, which is one of the most-widely used safe strains in synthetic biology, and it will not do any harm to human or environment.

Hardware

With 4 different sensors, the device can collect related data on temperature, pH, oxidization-reduction potential (ORP) and electrical conductivity of the cooling water. The implemented blue LED light trigger the expression of suicide gene, which can be turned on or off manually. That is to say, if people think it’s time to renew the recycle cooling water or the risks of blockage and corrosion are removed, they can kill the engineered E. coli by turning on the blue light manually to ensure the highest level of biosafety.

Discussion

Figure1.1 OD600 reference point tab

On top of being trained by supervisors on biosafety rules, Iron Guardian will also gather together to have discussion on biosafety issues. Based on the Safety Form we filled before, when we have problems or make progress in the lab, we will seriously reflect from the view of biosafety, in order that we can keep improving ourselves.