Difference between revisions of "Team:Jiangnan"

 
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{{Jiangnan}}
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<link rel="stylesheet" href="https://2018.igem.org/Team:Jiangnan/CSSmaterializecss?action=raw&ctype=text/css">
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<style type="text/css">
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.JTp{
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    display: block;
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<h1> Welcome to iGEM 2018! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<h3>Before you start</h3>
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<p> Please read the following pages:</p>
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<ul>
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.rubberBand{
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<li> <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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animation-duration: 2s;
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<li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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        <style type="text/css">
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.Jnav{position: fixed;top: 17px;opacity: 1;background-color: rgba(255, 255, 255, 0.2); width: 100%;z-index:999;}
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<div class="Jnav">
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<div class="Jnavtitle">
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<a href="https://2018.igem.org/Team:Jiangnan/Safety">Safety</a>
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</div>
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<div class="Jnavtitle">
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<a href="https://2018.igem.org/Team:Jiangnan/Hardware">Hardware</a>
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</div>
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<div class="Jnavtitle" onmouseover="Jnavshow(this)" onmouseleave="Jnavhide(this)">
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<a href="https://2018.igem.org/Team:Jiangnan/Team">Team</a>
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<div class="Jnavdrag">
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<ul>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Team">Team Members</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Attributions">Attribution</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Collaborations">Collaboration</a></li>
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</ul>
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</div>
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</div>
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<div class="Jnavtitle" onmouseover="Jnavshow(this)" onmouseleave="Jnavhide(this)">
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<a href="https://2018.igem.org/Team:Jiangnan/Human_Practices">Human Practice</a>
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<div class="Jnavdrag">
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<ul>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Human_Practices">Overview</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Silver">Silver</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Entrepreneurship">Gold</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Public_Engagement">Public Engagement</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Entrepreneurship">Entrepreneurship</a></li>
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</ul>
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</div>
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</div>
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<div class="Jnavtitle">
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<a href="https://2018.igem.org/Team:Jiangnan/Model">Model</a>
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</div>
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<div class="Jnavtitle" onmouseover="Jnavshow(this)" onmouseleave="Jnavhide(this)">
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<a href="https://2018.igem.org/Team:Jiangnan/Notebook">Notebook</a>
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<div class="Jnavdrag">
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<ul>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Notebook">Lab Book</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Protocol">Protocol</a></li>
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</ul>
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</div>
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</div>
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<div class="Jnavtitle" onmouseover="Jnavshow(this)" onmouseleave="Jnavhide(this)">
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<a href="https://2018.igem.org/Team:Jiangnan">Project</a>
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<div class="Jnavdrag">
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<ul>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Background">Background</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Design">Design</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Demonstrate">Demonstration</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Results">Result</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Parts">Part</a></li>
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</ul>
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</div>
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</div>
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<div class="navlogo" style="float: left;width: 20%;text-align: center;">
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<a href="https://2018.igem.org/Team:Jiangnan"><img src="https://static.igem.org/mediawiki/2018/d/d7/T--Jiangnan--igemJN_logo.png" style="width: 3em;"></a>
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</div>
 
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<h3> Styling your wiki </h3>
 
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
 
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
 
  
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<h3> Uploading pictures and files </h3>
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<img src="https://static.igem.org/mediawiki/2018/0/0a/T--Jiangnan--main_top.png" width="100%">
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<p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
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<div style="position: absolute;top: 4em;width: 100%;">
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<div style="text-align: center;">
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<img src="https://static.igem.org/mediawiki/2018/a/a1/T--Jiangnan--main_logo.png" width="10%">
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<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<h4 style="color: white;">Project Description</h4>
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<div style="margin-bottom: 0; width: 60%; display: inline; position: relative;">
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<div class="button_link">
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<p style="text-align: center; color: white;">The limiting factors of most vaccine companies, according to our previous investigation and survey, are
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<a href="https://2018.igem.org/Special:Upload">
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mulitifaceted costs in actual production process and long product lift cycle and other issues due
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UPLOAD FILES
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to complex application process of new vaccines(cell line).<br/>
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</a>
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The goal of our project is to enable three features of our chassis cell, i.e.,high titer, suspension cultivation, and
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broad spectrum to reduce the production cost increase the yield of viruses for vaccine production. The chassis cell we used here is
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MDBK cell.</p>
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</div>
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<div style="width: 33.3%;float: left;">
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<a href="#broad"><img class="animated infinite pulse" src="https://static.igem.org/mediawiki/2018/0/06/T--JiangNan--broad.png" width="50%"></a>
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<div><h4 style="color: white">Broad Spectrum</h4></div>
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</div>
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<div style="width: 33.3%;float: left;">
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<a href="#sus"><img class="animated infinite swing" src="https://static.igem.org/mediawiki/2018/c/cf/T--Jiangnan--main--sus.png" width="50%"></a>
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<div><h4 style="color: white">Suspension Culture</h4></div>
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</div>
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<div style="width: 33.3%;float: left;">
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<a href="#high"><img class="animated infinite rubberBand" src="https://static.igem.org/mediawiki/2018/9/96/T--Jiangnan--main--high.png" width="50%"></a>
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<div><h4 style="color: white">High Titer</h4></div>
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</div>
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</div>
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</div>
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</div>
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<div id="broad" class="row">
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<div class="col s7 offset-s1">
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<h5 style="color: #2e7d32">Broad Spectrum</h5>
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<p>Through text mining, we classified cell receptors according to the Baltimore subtyping of viruses and summarized the primary receptors mediating the entry of different types of viruses. After systematic analysis, we aim to express Nectin 4 and Tfr on our chassis cells to make them possible to a broad spectrum of viruses.</p>
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</div>
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<div class="col s3">
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<img src="https://static.igem.org/mediawiki/2018/4/42/T--Jiangnan--main--broadmove.png" width="100%">
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</div>
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</div>
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<div id="sus" class="row">
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<div class="col s3 offset-s1">
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<img src="https://static.igem.org/mediawiki/2018/0/00/T--Jiangnan--main--susmove.png" width="100%">
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</div>
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<div class="col s7">
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<h5 style="text-align: right;color: #ff9100">Suspension Culture</h5>
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<p>Through high throughput sequencing of two pairs of cells with and without the suspension feature, we found a panel of genes responsible for the suspension feature of cells following network construction using computational approach. The top gene was functionally validated before applied to the chassis cell for genomic modulation.</p>
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</div>
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</div>
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<div id="high" class="row">
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<div class="col s7 offset-s1">
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<h5 style="color: #03a9f4">High Titer</h5>
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<p>Through bioinformatics analysis and mathematical modeling, we constructed the network regulating virus titer of cells. The top gene was selected, functionally validated in vitro and used for genomic modulation in the chassis cells to enable them the feature of increased titer. On the other hand, we manufactured a device to generate cold atmospheric plasma, using which cell titer was further increased in response to plasma irradiation.</p>
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</div>
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<div class="col s3">
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<img src="https://static.igem.org/mediawiki/2018/1/17/T--Jiangnan--main--highmove.png" width="100%">
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</div>
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</div>
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<img src="https://static.igem.org/mediawiki/2018/b/b6/T--Jiangnan--thanks.png" width="100%" style="margin-bottom: -1em;">
 
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<h3> Wiki template information </h3>
 
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
 
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
 
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<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
 
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<div class="button_link">
 
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<a href="https://2018.igem.org/wiki/index.php?title=Team:Jiangnan&action=edit">
 
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EDIT PAGE
 
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<h3>Tips</h3>
 
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
 
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<ul>
 
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
 
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<li>Be clear about what you are doing and how you plan to do this.</li>
 
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
 
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
 
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
 
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
 
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<li>Have lots of fun! </li>
 
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<h3>Inspiration</h3>
 
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
 
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<ul>
 
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
 
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
 
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
 
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
 
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
 
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
 
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Latest revision as of 03:01, 18 October 2018

Safety
Hardware
Team
Human Practice
Model
Notebook
Project

Project Description

The limiting factors of most vaccine companies, according to our previous investigation and survey, are mulitifaceted costs in actual production process and long product lift cycle and other issues due to complex application process of new vaccines(cell line).
The goal of our project is to enable three features of our chassis cell, i.e.,high titer, suspension cultivation, and broad spectrum to reduce the production cost increase the yield of viruses for vaccine production. The chassis cell we used here is MDBK cell.

Broad Spectrum

Suspension Culture

High Titer

Broad Spectrum

Through text mining, we classified cell receptors according to the Baltimore subtyping of viruses and summarized the primary receptors mediating the entry of different types of viruses. After systematic analysis, we aim to express Nectin 4 and Tfr on our chassis cells to make them possible to a broad spectrum of viruses.

Suspension Culture

Through high throughput sequencing of two pairs of cells with and without the suspension feature, we found a panel of genes responsible for the suspension feature of cells following network construction using computational approach. The top gene was functionally validated before applied to the chassis cell for genomic modulation.

High Titer

Through bioinformatics analysis and mathematical modeling, we constructed the network regulating virus titer of cells. The top gene was selected, functionally validated in vitro and used for genomic modulation in the chassis cells to enable them the feature of increased titer. On the other hand, we manufactured a device to generate cold atmospheric plasma, using which cell titer was further increased in response to plasma irradiation.